LOCUS       NM_205128               1975 bp    mRNA    linear   VRT 27-APR-2025
DEFINITION  Gallus gallus occludin (OCLN), mRNA.
ACCESSION   NM_205128
VERSION     NM_205128.1
KEYWORDS    RefSeq.
SOURCE      Gallus gallus (chicken)
  ORGANISM  Gallus gallus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
            Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes;
            Phasianidae; Phasianinae; Gallus.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Tang,H., Finn,R.D. and Thomas,P.D.
  TITLE     TreeGrafter: phylogenetic tree-based annotation of proteins with
            Gene Ontology terms and other annotations
  JOURNAL   Bioinformatics 35 (3), 518-520 (2019)
   PUBMED   30032202
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Stephens,C.S. and Johnson,P.A.
  TITLE     Occludin expression and regulation in small follicles of the layer
            and broiler breeder hen
  JOURNAL   Gen Comp Endocrinol 248, 106-113 (2017)
   PUBMED   28238709
  REMARK    GeneRIF: the level of feed intake is related to or may directly
            regulate OCLN mRNA expression or may have an indirect effect
            through paracrine or autocrine factors in the ovary.
REFERENCE   3  (bases 1 to 1975)
  AUTHORS   Burge,S., Kelly,E., Lonsdale,D., Mutowo-Muellenet,P., McAnulla,C.,
            Mitchell,A., Sangrador-Vegas,A., Yong,S.Y., Mulder,N. and Hunter,S.
  TITLE     Manual GO annotation of predictive protein signatures: the InterPro
            approach to GO curation
  JOURNAL   Database (Oxford) 2012, bar068 (2012)
   PUBMED   22301074
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1975)
  AUTHORS   Zhang,B., Shao,Y., Liu,D., Yin,P., Guo,Y. and Yuan,J.
  TITLE     Zinc prevents Salmonella enterica serovar Typhimurium-induced loss
            of intestinal mucosal barrier function in broiler chickens
  JOURNAL   Avian Pathol 41 (4), 361-367 (2012)
   PUBMED   22834550
  REMARK    GeneRIF: Dietary Zn supplementation appeared to alleviate the loss
            of intestinal mucosal barrier function induced by S. Typhimurium
            challenge and the partial mechanism might be related to the
            increased expression of occludin and claudin-1 in broiler chickens.
REFERENCE   5  (bases 1 to 1975)
  AUTHORS   Elias,B.C., Suzuki,T., Seth,A., Giorgianni,F., Kale,G., Shen,L.,
            Turner,J.R., Naren,A., Desiderio,D.M. and Rao,R.
  TITLE     Phosphorylation of Tyr-398 and Tyr-402 in occludin prevents its
            interaction with ZO-1 and destabilizes its assembly at the tight
            junctions
  JOURNAL   J Biol Chem 284 (3), 1559-1569 (2009)
   PUBMED   19017651
  REMARK    GeneRIF: a unique motif in the occludin sequence that is involved
            in the regulation of ZO-1 binding by reversible phosphorylation of
            specific Tyr residues.
REFERENCE   6  (bases 1 to 1975)
  AUTHORS   Cordenonsi,M., Turco,F., D'atri,F., Hammar,E., Martinucci,G.,
            Meggio,F. and Citi,S.
  TITLE     Xenopus laevis occludin. Identification of in vitro phosphorylation
            sites by protein kinase CK2 and association with cingulin
  JOURNAL   Eur J Biochem 264 (2), 374-384 (1999)
   PUBMED   10491082
REFERENCE   7  (bases 1 to 1975)
  AUTHORS   Furuse,M., Fujita,K., Hiiragi,T., Fujimoto,K. and Tsukita,S.
  TITLE     Claudin-1 and -2: novel integral membrane proteins localizing at
            tight junctions with no sequence similarity to occludin
  JOURNAL   J Cell Biol 141 (7), 1539-1550 (1998)
   PUBMED   9647647
REFERENCE   8  (bases 1 to 1975)
  AUTHORS   Haskins,J., Gu,L., Wittchen,E.S., Hibbard,J. and Stevenson,B.R.
  TITLE     ZO-3, a novel member of the MAGUK protein family found at the tight
            junction, interacts with ZO-1 and occludin
  JOURNAL   J Cell Biol 141 (1), 199-208 (1998)
   PUBMED   9531559
REFERENCE   9  (bases 1 to 1975)
  AUTHORS   Furuse,M., Hirase,T., Itoh,M., Nagafuchi,A., Yonemura,S.,
            Tsukita,S. and Tsukita,S.
  TITLE     Occludin: a novel integral membrane protein localizing at tight
            junctions
  JOURNAL   J Cell Biol 123 (6 Pt 2), 1777-1788 (1993)
   PUBMED   8276896
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from D21837.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D21837.1, SRR13267649.10767.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA103992290, SAMEA103992323
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Gallus gallus"
                     /mol_type="mRNA"
                     /db_xref="taxon:9031"
                     /chromosome="28"
                     /map="28"
     gene            1..1975
                     /gene="OCLN"
                     /note="occludin"
                     /db_xref="CGNC:49614"
                     /db_xref="GeneID:396026"
     exon            1..13
                     /gene="OCLN"
                     /inference="alignment:Splign:2.1.0"
     exon            14..701
                     /gene="OCLN"
                     /inference="alignment:Splign:2.1.0"
     CDS             21..1535
                     /gene="OCLN"
                     /EC_number="2.1.1.67"
                     /codon_start=1
                     /product="occludin"
                     /protein_id="NP_990459.1"
                     /db_xref="CGNC:49614"
                     /db_xref="GeneID:396026"
                     /translation="

MFSKKSYDGPPAGYGPPTGYGAPTADYGYGSPPPGSYYVDDAPQLFYKWTSPPGAVRGLQAGVLVLCIAIFACVASTLAWDYGYGLGGAYGTGLGGFYGSNYYGSGLSYSYGYGGYYGGVNQRTANGFMIAMAVLCFLAQLGLLVAALSKSGATRSRRFYLAVLVLSAVLAFVMLIASIVYIMGVNPQAQMSSGYYYSPLLAMCSQAYGSTYLNQYIYHYCTVDPQEAVAAVCGFLIVILLCLICFFAQKTRSKIWRYGKANIYWDRAPVVQEGPDVEEWVKNVADGASVQDETATLAYSEKPTSPVAAPPYSYVPPPSAGYYPSGTYSSRGDQPDRALSASPVHGEEEEEKGKDQPSRPPARRGRRRRRNPELDESQYETDYTTAVESSDERDQEQWASLYPPITSDGARQRYKQEFDTDLKRYKQLCAEMDSINDRLNQLSRRLDSITEDSPQYQDVAEEYNQLKDLKRSPDYQSKKQESKVLRNKLFHIKRMVSAYDKVRG"

     misc_feature    162..761
                     /gene="OCLN"
                     /note="Membrane-associating domain; Region: MARVEL;
                     pfam01284"
                     /db_xref="CDD:366555"
     misc_feature    192..260
                     /gene="OCLN"
                     /note="propagated from UniProtKB/Swiss-Prot (Q91049.1);
                     transmembrane region"
     misc_feature    390..464
                     /gene="OCLN"
                     /note="propagated from UniProtKB/Swiss-Prot (Q91049.1);
                     transmembrane region"
     misc_feature    495..569
                     /gene="OCLN"
                     /note="propagated from UniProtKB/Swiss-Prot (Q91049.1);
                     transmembrane region"
     misc_feature    702..767
                     /gene="OCLN"
                     /note="propagated from UniProtKB/Swiss-Prot (Q91049.1);
                     transmembrane region"
     misc_feature    990..1208
                     /gene="OCLN"
                     /note="propagated from UniProtKB/Swiss-Prot (Q91049.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    1155..1175
                     /gene="OCLN"
                     /note="propagated from UniProtKB/Swiss-Prot (Q91049.1);
                     Region: Interaction with TJP1.
                     /evidence=ECO:0000269|PubMed:19017651"
     misc_feature    1155..1157
                     /gene="OCLN"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0000269|PubMed:19017651; propagated from
                     UniProtKB/Swiss-Prot (Q91049.1); phosphorylation site"
     misc_feature    1167..1169
                     /gene="OCLN"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0000269|PubMed:19017651; propagated from
                     UniProtKB/Swiss-Prot (Q91049.1); phosphorylation site"
     misc_feature    1224..1523
                     /gene="OCLN"
                     /note="Occludin homology domain; Region: Occludin_ELL;
                     pfam07303"
                     /db_xref="CDD:462140"
     exon            702..860
                     /gene="OCLN"
                     /inference="alignment:Splign:2.1.0"
     exon            861..1015
                     /gene="OCLN"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1219
                     /gene="OCLN"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..1391
                     /gene="OCLN"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1433
                     /gene="OCLN"
                     /inference="alignment:Splign:2.1.0"
     exon            1434..1975
                     /gene="OCLN"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

caaagcggcggagggccaccatgttcagcaagaagtcctacgacggcccccccgcggggtacggcccccccacggggtacggcgcccccacggctgattacggctacgggtctccgccgccgggctcctactacgtggacgacgctccgcagctcttctacaagtggacgtcgccgcccggcgcggtgcgggggctgcaggcgggggtcctcgtgctgtgcatcgccatcttcgcctgcgtcgcttccacgctcgcctgggattacggctacggcctggggggggcgtacggcaccgggctgggggggttctacggctccaactactacggcagcgggctgagctacagctacggctacgggggctactacggaggggtgaaccagcgcacggccaacggcttcatgatcgccatggccgtgctgtgcttcctggcccagctggggctgctggtggcggcgctcagcaaatccggggccacgcgctcgcggcgcttctacctggccgtgctggtgctgagcgccgtgctggccttcgtcatgctcatcgcctccatcgtctacatcatgggcgtcaacccgcaggcgcagatgtccagcggttactactacagccccttgttggccatgtgcagccaggcctacggcagcacctacctcaaccagtacatctaccactactgcaccgtggacccccaggaggctgtggctgctgtctgtgggttcctcatcgtcatcctgctctgcctcatctgcttcttcgcccagaagacgcgcagtaagatctggcgctacggcaaagccaacatctactgggaccgcgcgcccgtggtgcaggaggggcctgacgtggaggagtgggtgaagaacgtggcggatggggccagcgtgcaggacgagacggccacgcttgcctactcggagaagcccaccagccctgtcgccgcccccccctacagctacgtgcccccccccagcgctgggtactacccctcgggcacctacagcagccggggcgaccagccggaccgggccctcagtgccagccctgtgcatggggaggaggaggaggagaaggggaaggatcagcccagcagaccgcccgcccgccggggccgccgccgccgccgtaaccccgagttggatgagtcccagtatgagaccgactacaccacggccgtggagtccagtgatgagcgggaccaggagcagtgggccagtctgtacccccccatcacgtcggacggcgcccgccagcgctacaagcaggagttcgacaccgacctgaagcgctacaagcagctctgtgctgagatggacagcatcaacgaccgcctcaatcagctcagccgacggctcgacagcatcaccgaggacagccctcaataccaggatgtggcagaggagtacaatcagctcaaagacctgaagcggagcccagactaccaaagcaagaagcaggagagcaaagtgctgcgcaacaagctcttccacatcaagcgcatggtgagcgcctacgacaaggtgcgggggtaaccagcaccagcaggaggggggggcacccccacccccccacccaaagactgttgtgcattttgtaccttcttcttaaaagaaaaaacacactcaatcacatccaccccccccccaaaggggtcaccccccccaacccccccttaacccctcccactccatccactgtgaatgcacccactgagtgttgggggctgccccccccccccgtgctgttattatggtagtggggggggggggggagctgtgtaaggcccacacctctgggaagggctgaggtctgcaacagcatcacagccgccctggcactgtgggtaccagaatggtaccctgagcacacagctcaaggtgggggggggagcatcccttgggatggggggggggggcagtgcctttgggggagagggggggtcagggctttttgggaaggcattttgtaaggc

//