LOCUS       NM_001396481            3659 bp    mRNA    linear   VRT 23-SEP-2023
DEFINITION  Gallus gallus argonaute 4, RISC catalytic component (AGO4),
            transcript variant 3, mRNA.
ACCESSION   NM_001396481
VERSION     NM_001396481.1
KEYWORDS    RefSeq.
SOURCE      Gallus gallus (chicken)
  ORGANISM  Gallus gallus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
            Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes;
            Phasianidae; Phasianinae; Gallus.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Burge S, Kelly E, Lonsdale D, Mutowo-Muellenet P, McAnulla C,
            Mitchell A, Sangrador-Vegas A, Yong SY, Mulder N and Hunter S.
  TITLE     Manual GO annotation of predictive protein signatures: the InterPro
            approach to GO curation
  JOURNAL   Database (Oxford) 2012, bar068 (2012)
   PUBMED   22301074
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caldwell RB, Kierzek AM, Arakawa H, Bezzubov Y, Zaim J, Fiedler P,
            Kutter S, Blagodatski A, Kostovska D, Koter M, Plachy J, Carninci
            P, Hayashizaki Y and Buerstedde JM.
  TITLE     Full-length cDNAs from chicken bursal lymphocytes to facilitate
            gene function analysis
  JOURNAL   Genome Biol 6 (1), R6 (2005)
   PUBMED   15642098
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAENSK010000339.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13267661.2873.1,
                                           SRR13267654.121578.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA103992290,
                                           SAMEA103992323 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-250               JAENSK010000339.1  4255376-4255625     c
            251-416             JAENSK010000339.1  4250705-4250870     c
            417-537             JAENSK010000339.1  4250117-4250237     c
            538-719             JAENSK010000339.1  4249749-4249930     c
            720-856             JAENSK010000339.1  4249238-4249374     c
            857-991             JAENSK010000339.1  4249020-4249154     c
            992-1079            JAENSK010000339.1  4248755-4248842     c
            1080-1227           JAENSK010000339.1  4248226-4248373     c
            1228-1347           JAENSK010000339.1  4248028-4248147     c
            1348-1476           JAENSK010000339.1  4247677-4247805     c
            1477-1610           JAENSK010000339.1  4247384-4247517     c
            1611-1795           JAENSK010000339.1  4246792-4246976     c
            1796-1955           JAENSK010000339.1  4246005-4246164     c
            1956-2271           JAENSK010000339.1  4245244-4245559     c
            2272-2406           JAENSK010000339.1  4244916-4245050     c
            2407-2508           JAENSK010000339.1  4244014-4244115     c
            2509-2708           JAENSK010000339.1  4243583-4243782     c
            2709-3659           JAENSK010000339.1  4242534-4243484     c
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Gallus gallus"
                     /mol_type="mRNA"
                     /db_xref="taxon:9031"
                     /chromosome="23"
                     /map="23"
     gene            1..3659
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /note="argonaute 4, RISC catalytic component"
                     /db_xref="CGNC:66346"
                     /db_xref="GeneID:419629"
     exon            1..250
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    187..189
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /note="upstream in-frame stop codon"
     exon            251..416
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            417..537
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     CDS             433..2817
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /note="isoform 2 is encoded by transcript variant 3;
                     protein argonaute-4; argonaute RISC catalytic component 4;
                     eukaryotic translation initiation factor 2C, 4"
                     /codon_start=1
                     /product="protein argonaute-4 isoform 2"
                     /protein_id="NP_001383410.1"
                     /db_xref="CGNC:66346"
                     /db_xref="GeneID:419629"
                     /translation="

MVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDMEVTLPGEGKDQTFKVSIQWVSVVSLQLLLEALAGHLNEVPEDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKLTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQETSQELLYSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTQHTMYFA"

     misc_feature    442..696
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:435368"
     misc_feature    727..879
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:430160"
     misc_feature    880..1242
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1036..1038,1081..1083,1123..1125,1135..1137,
                     1189..1191,1210..1212,1216..1218)
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1381..2688
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1792..1794,1804..1806,1840..1851,1858..1860,
                     1882..1884,1891..1893,1903..1905,1915..1917)
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1996..1998,2002..2004,2242..2244,2656..2658)
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /note="active site"
                     /db_xref="CDD:240015"
     misc_feature    2704..2769
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /note="propagated from UniProtKB/Swiss-Prot (Q5ZMW0.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     exon            538..719
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            720..856
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            857..991
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            992..1079
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            1080..1227
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            1228..1347
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            1348..1476
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1610
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            1611..1795
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            1796..1955
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            1956..2271
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            2272..2406
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            2407..2508
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            2509..2708
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
     exon            2709..3659
                     /gene="AGO4"
                     /gene_synonym="EIF2C4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

acgcgccgccgcttggcatgctgggaagtgcagtcctgaggcaaggcgggaggacggcggtgaggggctcacgggcagctgaggacgcgccgccgtctcgctcggtgctcatcccaaccctcctccgcaccgcggtcaccggagctgcaagacccccggaagccgccagccgtgtgcgcggagcggtaggactcacctcaacgggagccgccgccatggcccgggccggacgcagtcacagcgctgccgcggcccccagcaagccttttccagcccccccgtcgcccaggcctgggaactgttgggaaacccatccgcctcctagccaaccactttcaggttcagatccccaagattgatgtttatcactatgatgtggatatcaaaccagaaaaacgaccccgaagagtgaacagagaagtggtggatactatggtgagacacttcaagatgcagatatttggtgaccggcagcctggatacgatgggaagcggaacatgtacactgcacacccattacccattggcagggacagagtggatatggaggtgacacttccaggagaggggaaggaccagactttcaaagtgtccattcagtgggtgtcagttgtcagccttcagttgctgttggaagctctggcagggcatttgaatgaagttcctgaagattctgtacaagcacttgatgtaattacacggcaccttccgtccatgaggtatactcctgtgggtcgctcctttttttccccccctgaaggctattaccaccctctgggtggtggcagggaggtctggtttggtttccaccagtctgtcaggcctgccatgtggaacatgatgctcaacattgatgtatcagcaactgctttctatcgtgctcagcctatcattgagttcatgtgtgaggtcttggacattcagaacatcaatgaacaaaccaagcctctgacagactcccaacgtgtcaagtttaccaaagaaatcagaggtctaaaagtagaggttactcactgtggccagatgaagagaaagtatcgagtttgcaatgttactcgtcgaccagccagccatcagacgtttcctctgcagttggaaaatgggcaggctatggagtgtacagtagctcagtattttaagcagaagtacagtctgcagctaaaatatcctcatcttccctgtctccaagtaggacaggagcagaaacacacatacttaccgcttgaggtgtgtaacatagtggcaggccagaggtgtatcaagaagttaacagacaatcagacatcaactatgataaaagcgactgcaagatctgcacccgaccgacaggaagaaatcagcagacttgtgaaaagtaacagtatggttggtggacctgatccatatctgaaggagtttggtattgttgtccataatgaaatgacagagttgacaggcagagttttgccagcgccaatgctgcaatatggaggcaggaacaagactgtggccacaccaaaccaaggtgtgtgggacatgagagggaaacagttctatgctggcattgagattaaagtttgggctgttgcctgttttgctcctcaaaaacaatgcagggaagacttactaaagagttttacagaccagctgcgcaagatctccaaggacgcagggatgccgatccaaggccagccctgtttctgtaaatacgcccagggtgcagacagtgtggagcccatgttcaaacatttaaaactgacttatgttggtctgcagctgattgtggtgattctacctggaaagacacccgtgtatgctgaagtaaagcgagttggcgacactcttcttggcatggccactcagtgtgttcaggtaaagaatgtggtgaaaacctcaccacaaacactgtccaacctgtgtctgaaaataaatgcaaagcttggaggaatcaacaacgtgctcgtacctcatcaaaggccctcggtgttccagcagccagtgatctttttgggagcagatgtcactcaccctcctgctggggatggaaagaagccttccatagctgctgtggtcggcagcatggatggccatcctagccgttactgtgccacagtgcgagtgcagacctcccgtcaggagacctcccaggaactgctgtacagccaggaggtgatacaggacctgacaaacatggtgcgagaactgctgatacagttttacaaatccacacgtttcaagcctacaagaatcatttactacagaggaggagtatcagaagggcagatgaagcaggtagcttggccagaacttatagcaatcagaaaggcctgtattagtttggaagaagactacagaccaggaataacctacatcgttgtgcagaaaagacaccacaccaggctgttctgtgccgacaaaactgaaagggtgggtaagagtggcaacgtaccagcgggcactactgtggacagcacgatcacgcacccttctgaatttgacttttacctctgtagccacgcaggaattcagggaaccagccggccctcccactatcaggtcctgtgggatgacaactgtttcactgcagatgagctacagctgctgacctatcagctgtgtcacacgtatgtgcgctgtacgcgatcagtctctattccagcacctgcatactatgccagactggtagcatttagggctagataccatcttgtggacaaggatcacgacagtgctgaaggcagccacgtgtcaggacagagcaatggccgtgatcctcaggctctggcaaaggcagtgcagatccaccatgatactcaacatacaatgtattttgcttgagagcatctgggaagatgaggcaaagccaacaactcgttcagtcctgacatgttttcaatgcctactgctatagctagggcccggtttctttcagactgcccactaccagcagctctgaacagtcgcactgaatctgtacttcaaacaatgtctgatgcaccgacacaattgagccattcgtttcgttattttacgtcatagaaattcatagactgtcttttctgatgcattggactaaatttttacctcaaagcgcaaaaggccgatcatcccgacgtttttaaaggacttggcacgaaaggtttctattgaatactttgctagctctggtctttaggttcccttctcatcccattagctgatggtgacgactgattctccgtcctcacgcgctgcttagtgagtatgcgtagtatggcgaagttctcctccagttcaatgtaatcgttctcttgtgtggggccatagatttttttaatggagattttagacttcactactgtaaatgccttttaacagacgtaactttcacaggtcttgcagtttttgtcggttttactaatgacctttttaacctgcagactcgggctgcaaagcaattgcactattctgcgagttcagatcggtggttcctgttgtgaatgtgtgacgtgcccaggtggtggtgcctggcagtagctttcaattgtaacgcctacagataatgctgccatggcgtcgtgacaaacactgaaccatcgttttcccccctctaaaaaaacaaaacaaaaaaaaaaacgaaaaaaaaaaaaacaaaaaaaggatttaaaaaaaaaatagaaaaaaaaatacaaaaaaaaaaa

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