LOCUS       NM_001389549           12501 bp    mRNA    linear   VRT 23-SEP-2023
DEFINITION  Gallus gallus argonaute 2, RISC catalytic component (AGO2), mRNA.
ACCESSION   NM_001389549
VERSION     NM_001389549.2
KEYWORDS    RefSeq.
SOURCE      Gallus gallus (chicken)
  ORGANISM  Gallus gallus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
            Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes;
            Phasianidae; Phasianinae; Gallus.
REFERENCE   1  (bases 1 to 12501)
  AUTHORS   Burge S, Kelly E, Lonsdale D, Mutowo-Muellenet P, McAnulla C,
            Mitchell A, Sangrador-Vegas A, Yong SY, Mulder N and Hunter S.
  TITLE     Manual GO annotation of predictive protein signatures: the InterPro
            approach to GO curation
  JOURNAL   Database (Oxford) 2012, bar068 (2012)
   PUBMED   22301074
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAENSK010000044.1.
            
            On Dec 1, 2021 this sequence version replaced NM_001389549.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13267659.218747.1,
                                           SRR13267656.6950.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA103992290,
                                           SAMEA103992323 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-135               JAENSK010000044.1  24432055-24432189   c
            136-331             JAENSK010000044.1  24414758-24414953   c
            332-452             JAENSK010000044.1  24410420-24410540   c
            453-634             JAENSK010000044.1  24405165-24405346   c
            635-771             JAENSK010000044.1  24403730-24403866   c
            772-906             JAENSK010000044.1  24403459-24403593   c
            907-994             JAENSK010000044.1  24400543-24400630   c
            995-1142            JAENSK010000044.1  24399334-24399481   c
            1143-1262           JAENSK010000044.1  24399033-24399152   c
            1263-1385           JAENSK010000044.1  24398723-24398845   c
            1386-1519           JAENSK010000044.1  24397408-24397541   c
            1520-1704           JAENSK010000044.1  24396368-24396552   c
            1705-1864           JAENSK010000044.1  24394609-24394768   c
            1865-1955           JAENSK010000044.1  24392780-24392870   c
            1956-2150           JAENSK010000044.1  24391920-24392114   c
            2151-2285           JAENSK010000044.1  24391248-24391382   c
            2286-2387           JAENSK010000044.1  24389531-24389632   c
            2388-2587           JAENSK010000044.1  24389026-24389225   c
            2588-12501          JAENSK010000044.1  24378772-24388685   c
FEATURES             Location/Qualifiers
     source          1..12501
                     /organism="Gallus gallus"
                     /mol_type="mRNA"
                     /db_xref="taxon:9031"
                     /chromosome="2"
                     /map="2"
     gene            1..12501
                     /gene="AGO2"
                     /note="argonaute 2, RISC catalytic component"
                     /db_xref="CGNC:64972"
                     /db_xref="GeneID:100858102"
     exon            1..135
                     /gene="AGO2"
                     /inference="alignment:Splign:2.1.0"
     CDS             105..2696
                     /gene="AGO2"
                     /EC_number="3.1.26.n2"
                     /note="argonaute RISC catalytic component 2"
                     /codon_start=1
                     /product="protein argonaute-2"
                     /protein_id="NP_001376478.1"
                     /db_xref="CGNC:64972"
                     /db_xref="GeneID:100858102"
                     /translation="

MYPGASTAGPVLAPPPPLPPPVQGYAFKPPPRPDFGTSGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVAIKWMSCVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQENGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKTFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYTGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA"

     misc_feature    390..614
                     /gene="AGO2"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:435368"
     misc_feature    642..794
                     /gene="AGO2"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:430160"
     misc_feature    795..1157
                     /gene="AGO2"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(951..953,996..998,1038..1040,1050..1052,1104..1106,
                     1125..1127,1131..1133)
                     /gene="AGO2"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1290..2567
                     /gene="AGO2"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1701..1703,1713..1715,1749..1760,1767..1769,
                     1791..1793,1800..1802,1812..1814,1824..1826)
                     /gene="AGO2"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1905..1907,1911..1913,2121..2123,2535..2537)
                     /gene="AGO2"
                     /note="active site"
                     /db_xref="CDD:240015"
     exon            136..331
                     /gene="AGO2"
                     /inference="alignment:Splign:2.1.0"
     exon            332..452
                     /gene="AGO2"
                     /inference="alignment:Splign:2.1.0"
     exon            453..634
                     /gene="AGO2"
                     /inference="alignment:Splign:2.1.0"
     exon            635..771
                     /gene="AGO2"
                     /inference="alignment:Splign:2.1.0"
     exon            772..906
                     /gene="AGO2"
                     /inference="alignment:Splign:2.1.0"
     exon            907..994
                     /gene="AGO2"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1142
                     /gene="AGO2"
                     /inference="alignment:Splign:2.1.0"
     exon            1143..1262
                     /gene="AGO2"
                     /inference="alignment:Splign:2.1.0"
     exon            1263..1385
                     /gene="AGO2"
                     /inference="alignment:Splign:2.1.0"
     exon            1386..1519
                     /gene="AGO2"
                     /inference="alignment:Splign:2.1.0"
     exon            1520..1704
                     /gene="AGO2"
                     /inference="alignment:Splign:2.1.0"
     exon            1705..1864
                     /gene="AGO2"
                     /inference="alignment:Splign:2.1.0"
     exon            1865..1955
                     /gene="AGO2"
                     /inference="alignment:Splign:2.1.0"
     exon            1956..2150
                     /gene="AGO2"
                     /inference="alignment:Splign:2.1.0"
     exon            2151..2285
                     /gene="AGO2"
                     /inference="alignment:Splign:2.1.0"
     exon            2286..2387
                     /gene="AGO2"
                     /inference="alignment:Splign:2.1.0"
     exon            2388..2587
                     /gene="AGO2"
                     /inference="alignment:Splign:2.1.0"
     exon            2588..12501
                     /gene="AGO2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

gctcgggcttctctccctcccttccctccctttccctccccttcggccgccgccgcggtagggacctggagcgaggcgccgcctccgccgccgcacacggagccatgtacccgggagccagcaccgccggacccgtgcttgcacctcctccaccgttgccaccaccagtacagggatatgccttcaaacctcctcctcgcccagattttgggacttctgggagaacaatcaaactgcaggccaacttctttgaaatggacattcctaaaatagatatctaccattacgagttggatataaagccagaaaaatgtcctagaagagttaatagagaaatagtggagcatatggttcagcactttaaaacacagatttttggggatcgcaagccagtgtttgatggaagaaaaaacctttatacagctatgccacttcccattggaagagataaagtggagttggaggtcacgttgccaggagaagggaaggacagaatatttaaggtggctataaagtggatgtcctgtgtgagtttgcaggcgttacatgatgctctttctggccggctgccgagcgtcccatttgaaaccatccaggcactggatgtagtgatgaggcacttgccttccatgagatatactccagtggggcgatctttttttacagcatcagaagggtgctcaaatccattaggtggtggcagagaggtttggtttggattccatcaatcagtcagaccttccttatggaaaatgatgttaaacattgatgtatcggcaactgcattttacaaagcacagccagttattgagtttgtatgtgaagttttggatttcaaaagcattgaagaacaacaaaaacctctgacagattcccaaagggtaaagtttaccaaagaaataaaaggtctaaaggtggagataacacactgtggacaaatgaaaagaaagtacagagtgtgcaacgtcaccagacgaccagcaagtcaccaaacattcccacttcagcaggagaatggacaaacagttgaatgcactgtagctcagtattttaaggacaggcacaaattagttttacgctatcctcaccttccatgtttacaagttgggcaggagcagaagcacacgtaccttcctcttgaggtgtgcaacatagtggcaggacaaagatgcataaaaaaactcactgacaatcagacttccactatgattcgagcaacagccagatcagcacctgaccgtcaggaagagattagcaaattgatgcggagtgcgagttttaacactgacccttatgttcgtgaatttgggataatggtgaaggatgaaatgacagatgtgactggtagagtcctacagcctccctcaatcctctatggaggcaggaataaagcaattgctacaccagttcaaggagtttgggacatgaggaataaacagtttcacactggaattgaaataaaggtttgggcaattgcatgctttgctccccagcgccaatgcactgaagtgcatcttaagaccttcacagagcaactgaggaagatttccagagatgcaggaatgccaatccaagggcagccatgcttctgtaaatatgcccagggagctgacagcgtggagcccatgttcaggcatctcaagaacacttacaccggactgcagctcgttgtagtaattttaccaggaaaaacaccagtctatgcggaggtgaagcgtgttggtgacacagtgctgggaatggctacgcagtgtgttcagatgaagaatgtgcagaggacaacacctcagactctgtctaacctctgcttaaaaataaacgtcaaattaggaggtgtaaataatattttactgccacagggaagaccaccagtattccaacagcctgtcatattccttggtgcagatgtaacccatcctccagctggagatggaaaaaagccctccattgctgcagttgtaggaagtatggatgctcatcccaatcgctactgtgccactgttcgggtccagcagcaccgccaagaaatcatccaagatttggctgccatggtcagagagctgctgattcagttctacaaatcaaccagatttaaaccaactcgtatcattttctacagagatggagtttccgaggggcagtttcagcaggttctccatcatgaattgctggctatcagagaagcatgtattaaactagagaaggattaccagcctggaattacatttatagttgtgcagaaaaggcatcacaccagactcttctgtacagataaaaatgagcgggttggaaaaagtgggaacattccagctggtaccacagtggacacaaaaatcacccatccatcagagtttgacttctacctgtgtagtcacgctggtattcagggaacaagcagaccttcgcattaccacgtcctctgggatgacaatcgtttctcttcagatgagctgcagattcttacctaccagctgtgtcatacatacgtacgctgcactcgttctgtttccatccctgcaccagcatattacgcgcacctggttgccttcagagccaggtatcatctggtggataaagaacacgacagtgctgaaggcagccacacttcgggtcagagtaatggcagagatcatcaagcacttgcaaaagcagttcaggttcaccaggacacgttgcgcactatgtactttgcttgacatgttttaatgtttagcgactcagtacagtaccgagttggattcacatgagaccagctgcacttagaccaacagttgcccaagctccagtgacagtcagcaatgagtgatgtatcttcatttttttttcccccctttttttgcaagaaagccttccgtccagaatttcagacttggttatgaactgcattcttctacaaagccccacgattgttggaaatgtggtttgccaaaatctctaagctgcctgttataaaacagacccatattttataattaaatcaagagctgtgatctcagggcttaggggaaaaaacaaaacacaaaactctccattgtttgctattcgtttaattgcttttaataatttggagagatctaattcattctaaagttaatgggctaaaatgctgcgaattagcttttaatacaaaggaaagcaaagcgtttttttgactatttatgaagccttgatttcattctggatgtatttccaatagttttcttctcaaaagaaaattgtgacaaaattctgctttccaactgtcttacaaagatttttctctccgtgtctaagggcaaatgtattttctgaagcttgttcttgaatgttttttatcgtgctgcatttaaaaatgtttttcatagacaaaaagaagaattttaaagcagaacgaacgaacttgtacctgaacttttagtaggtgcagtcagattggcaaaagagaaggtgccatttttaagaaaagaaaaagaatttaaagattttttttttttttttaatcatactgacagctaactttagaatttgtcatacaggactgtgacactctccaaagtctctaaggaatacgggtctgtggtgataaatacagtacaatcctctttcactgttatgtttgaattaatgtaaattttatatgtgtttcacagtattaatgtgatagactagatgctattatttatttttatttactaaggagtgctcattatacttctgttaacatatcaagaatgctttggatttagaaattaaaaccttatcgtgaggacgttactattcaaaagcaaaaatatgcaattgactttgatgcgggagttcagagctgcgtgcagaggacattgtgtagcccgtcctttggagaaaggcttttcgaagcgtacacacaacgtagcaggctttacctgtgcagtcaaagacgcacacgagaccaacgttctgcatccattcaagcacaagcaattgtatttaatttatgtgttggcggtcggtgaagctggagctctaagagagggaagtggtttgatgtttgtcgcgtggacagaagtcagagaatctatgcagtaaaggtaaatcttaggaaactgaagtttacttaggaagggaggtgaaaccttcattatgcgtgagagtttggccgttctcttaggagaagcattaattaactgataattattagacagacacttctgttagtttgcacactgggttcataaaaaaccgtagtacatatgaaaaccattgcactaatgatgatgccacaagcaggtgtagaagaaaaagaaaaaagcccaggaaatgtgtttatgctgactttgtttttgtttattttcataggaaaacttaaaatctgccctctcagtgtgtatgtaaggatgtcgttcttttccattttgctttgtcttttcccttttctcttagcagatgctcagtcactgttgtgcgtccatatacctctagctgctgagctgggccaggagctgtaaccaaggcagaggtctgtgccacgcagcagggagcctgtagctggctgcttagtaagctaggaagtgcccagcctttgctgcatgaagagttctaggtacgtaactcagtgcagattccaagcatcagcttgtagcagcgtgttggtgttgtttaggtgaaacaacgatgtcaatcctattagcagatgaagtgtgtttgtgttgttgtcatttgttggttagcctgccaggtcagacctttgtctcatgattgaatctagcttttcagtgatagacagtataacagaaagcaaactccgcgtcacgttgtctgtcttcccattttccagcagccaggctgtacgtacctctctcacatgtaccgctcacctgcaaaagcttttccacagttgacatgttgcacttctgtgtgtttgcttaatgcgtttcggatttcagattttaattcggcagagttttatatatgaatttatttttatatgaattactcttgacaaatacaaggaagatcaagcattttaaaactccagttatgtt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