LOCUS       XM_066503764            2532 bp    mRNA    linear   PLN 17-JUL-2024
DEFINITION  PREDICTED: Miscanthus floridulus protein argonaute 1B-like
            (LOC136508765), transcript variant X4, mRNA.
ACCESSION   XM_066503764
VERSION     XM_066503764.1
DBLINK      BioProject: PRJNA1133069
KEYWORDS    RefSeq.
SOURCE      Miscanthus floridulus
  ORGANISM  Miscanthus floridulus
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD
            clade; Panicoideae; Andropogonodae; Andropogoneae; Saccharinae;
            Miscanthus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_089580) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_019320115.1-RS_2024_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/10/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Miscanthus floridulus"
                     /mol_type="mRNA"
                     /cultivar="M001"
                     /db_xref="taxon:154761"
                     /chromosome="1"
                     /tissue_type="leaf"
                     /dev_stage="adult plants"
                     /geo_loc_name="China: Hunan"
     gene            1..2532
                     /gene="LOC136508765"
                     /note="protein argonaute 1B-like; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 6
                     Proteins"
                     /db_xref="GeneID:136508765"
     CDS             198..2396
                     /gene="LOC136508765"
                     /codon_start=1
                     /product="protein argonaute 1B-like isoform X4"
                     /protein_id="XP_066359861.1"
                     /db_xref="GeneID:136508765"
                     /translation="

MFLAGRQPDAPQGVIQLHNIVLRELSTARYCPVGRSFYSPNLGRRQQLGDGLETWRGFYQSIRPTQMGLSLNIDMSSTAFIEPLPVIEFVAQLLNRDISVRPLSDSDRVKIKKALRGVKVEVTHRGNMRRKYRISGLTSRATRELSCPIDDRGTVKTVVQHFLGTYGFSIQHTTLPCLQVGNQQRPNYLPMEVCKIVEGQRYSNRLTKKQITALLKVRPHEREKDISQTVHHNAYSEDPYAQEFGIRIDERLASVEARVLPPPKLKYHDSGRERDVWPRVGQWNMMNKKMVNGGRVSSWACINFSRNVQDGAARSFCHELALMCQASGVDFALEPVLPPRYARPEHVERALKGCYQDAMNILEPQGRELDLLIVILPDNNGSLYGDLKRICETDLGLVSQCCLTKYVLKANKQQYLSNISLKINVKVGGRNTVLVDALTRRIPLVSDVPTIIFGADVTHPHPGDDSSPSIAAVVASQDWPEVTKYPGLVSAQARRQELIQDLGGMVRELLISFWRATGQKPKRIIFYRDGVGEGQFCQVLLYELDAISRACASLESDYQPPVTFVVVQKRHHTRLFANNHNDPRAVDKSGNILPGTVVDSKICHPTQFDFYLCSHAGIQGTSRPAHYHVLWDENKFTADGLQTLTNNLCYTYARCTRSLSIVPPAYYAHLAAFRARYYLEPDTTDSGSMASGATPSRGPPPEARNTRAGVGNVAVRPLPALKENVKRVMFYC"

     misc_feature    291..443
                     /gene="LOC136508765"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    444..788
                     /gene="LOC136508765"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(591..593,636..638,669..671,681..683,735..737,
                     756..758,762..764)
                     /gene="LOC136508765"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    912..2234
                     /gene="LOC136508765"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1347..1349,1359..1361,1395..1406,1413..1415,
                     1440..1442,1449..1451,1461..1463,1473..1475)
                     /gene="LOC136508765"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1563..1565,1569..1571,1782..1784,2202..2204)
                     /gene="LOC136508765"
                     /note="active site"
                     /db_xref="CDD:240015"
     polyA_site      2532
                     /gene="LOC136508765"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

catttggatgggcgtctgcctgcatacgatggtaggaagaatctttatacagctggagcattgccatttacttcgaagacattcgtaattactctgcaagatgaggaagacagtcttggtggaggccaaggaggccaaagagggtatttcgggtggtgatcaaatttgctgctcacgctgatctccaccatctggctatgtttctagctgggaggcaaccagatgctcctcaaggagtaattcaattacataacattgtgctacgtgaattgtctactgccaggtattgtcctgttggtagatcattttattctcccaacttagggagacgtcagcaacttggtgatggtttggaaacttggcgtggtttctaccaaagcataaggcccacacagatgggtctttctctgaatattgatatgtcctctactgcatttattgagcccctccctgtgattgaatttgttgctcagcttcttaacagagatatctcagttagaccattgtctgattctgatcgtgtgaagattaaaaaagccctacgaggtgtgaaagtcgaggtcacacaccgtggaaacatgcgtaggaaatatcggatatctggcctcacttcacgagcaacaagggagttatcatgccctattgatgatcgtggtactgttaagactgtggtgcaacacttcctggggacttatggcttcagtattcagcacaccactttaccttgcttgcaagtgggcaatcagcaaagaccaaattatctgcctatggaggtctgtaagatagttgagggacagcgttactcaaatcgtcttactaagaaacagatcactgctctactgaaggtgcgtccccatgagcgtgagaaagacatctcgcagactgttcatcacaatgcctactctgaggatccttatgcccaggaatttggtataaggattgatgagcgtcttgcatccgttgaagctcgtgttctgcctcccccaaagctgaaataccatgatagtggcagagagagggatgtatggccaagagttggccagtggaatatgatgaataagaaaatggtcaatggtggtagagttagcagctgggcatgcattaacttctcacgaaatgtgcaagatggtgctgccaggagtttctgtcatgaactggctttgatgtgccaagcatcaggagtggattttgcacttgaacctgtgctgccccctcgctatgcgaggcctgaacatgttgaaagagcattaaagggatgctaccaagatgccatgaacatactcgagcctcagggccgagaacttgacttgctgattgtaatactgcctgacaataatggttctctttacggggatctcaaaaggatctgtgagactgatcttggattggtctcccaatgctgtttgactaaatatgttctcaaggcgaacaagcagcagtatctttcaaatatctccctgaaaataaatgtgaaggttgggggaaggaatacggtacttgttgatgctttgacaaggagaatcccccttgtcagtgacgtaccaactattatctttggtgctgatgtgacccatccccatcctggggacgattctagtccttccattgcagctgttgttgcttctcaggactggcctgaggttacaaagtatccaggattagtgagtgctcaagcccgtcgccaagaattgatacaggatcttggtggcatggtcagggaacttctcatttctttctggcgggcaactggacagaaaccaaagaggatcatattctacagggatggtgtcggtgagggacagttctgccaagttctgttgtatgaacttgatgccattagtagggcctgtgcatcattggagtcggattaccagcctccagttacttttgttgtggtccagaagcgtcatcacactaggttgtttgctaataatcacaatgacccacgtgctgttgataaaagtggaaacatactgcctggtactgtggtggactcaaagatctgccatccaactcaatttgatttctacctctgtagccatgctggcattcagggaacaagccgccctgcccattatcatgttctgtgggatgaaaacaaatttaccgctgatgggttgcaaactctcaccaacaacctgtgttacacgtatgctaggtgcactcgctcattatcaatcgtacctcctgcgtactatgctcacctggcagccttccgagctcgctactacctggagccggataccactgacagtgggtctatggcgagcggtgctacgccaagccgtggccctccaccagaggcacgcaacaccagggctggtgttgggaatgttgctgtgaggccattacctgccctcaaggaaaacgtgaagcgcgtcatgttctactgctaagattgatgctggcacacggttatgcaccgccgaagttaataatcagagctgtgaattggagctaccttttatattgaactatatgtatggtattgtaaactggttgcggtaactttgtatgccgctcttcttaaga

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