LOCUS       XM_012958238            3241 bp    mRNA    linear   VRT 18-DEC-2019
DEFINITION  PREDICTED: Xenopus tropicalis piwi-like RNA-mediated gene silencing
            4 (piwil4), mRNA.
ACCESSION   XM_012958238
VERSION     XM_012958238.2
DBLINK      BioProject: PRJNA205740
KEYWORDS    RefSeq.
SOURCE      Xenopus tropicalis (tropical clawed frog)
  ORGANISM  Xenopus tropicalis
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae;
            Xenopus; Silurana.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_030678.2) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Dec 18, 2019 this sequence version replaced XM_012958238.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Name             :: Xenopus tropicalis Annotation
                                           Release 104
            Annotation Version          :: 104
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 8.3
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Xenopus tropicalis"
                     /mol_type="mRNA"
                     /strain="Nigerian"
                     /db_xref="taxon:8364"
                     /chromosome="2"
                     /sex="female"
                     /tissue_type="liver and blood"
                     /dev_stage="adult"
                     /note="F17 inbred"
     gene            1..3241
                     /gene="piwil4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 6 ESTs, 10 Proteins, and 100%
                     coverage of the annotated genomic feature by RNAseq
                     alignments, including 2 samples with support for all
                     annotated introns"
                     /db_xref="GeneID:100486962"
                     /db_xref="Xenbase:XB-GENE-993661"
     CDS             63..2624
                     /gene="piwil4"
                     /codon_start=1
                     /product="piwi-like protein 4"
                     /protein_id="XP_012813692.2"
                     /db_xref="GeneID:100486962"
                     /db_xref="Xenbase:XB-GENE-993661"
                     /translation="

MALQEKPIDFSLKNKEPGLARDSPGQASKMSNPRPDAGSSKDLQGIGSGNGSGRDSGIGTISAGENRYVLAERGGRNGRNLQDFGINTRQTMAHVKDHKTGTSGAPVKLMTNLFGLTLPTQWKLYQYRVDFSPEFASRKLKTALLYSHGETLGRARAFHGEILFLSRNIGKLNHLESTTRKGETVKITITLTNQLPADSPVCIQFFGILFKKVFKSLNMYQVGRNYYNPADPVIIPQHRLMLWPGFVLSIMKLESQLALCADVSHKVLRNETVLDSMKALYSKVPKERFTDICEKELLGLVVLTRYNNKTYRVDDIDWSTKPTDSFPRKDGSEITYTDYYSQQYSINISDLQQPMLVSTLKAKKSDNPDAPRAVHLIPELCYLTGLSSRALSDFRLMKDLSMETQLDPERRQQRLQRLADSIQRTPEASAELEKWGLRMDKQIPVTGRIAPPERIFMRDNVCTPGSPADWARDTRNEKVICVAPLQNWLMLFSYRNADVAENLLQCLMKVASGMGFHMDMPRIVQVEDNPVSFLRTLQKNVTPDLQLVFCVIPTNQKDSYDSIKKFLSLDQPVPSQCVQNRTLGRQQMMMSVANKIAMQMICKLGGELWAVEIPLKALMVVGIDINKDAVNKSMSVVGFVASTNARLTRWHSRCIIQKTTSDFADCLKMCMKGAIQNWQRCNNDLPNRIVVYRDGVGDGQLKMVVDYEIPQLLSSFRETKTSYCPRLSVIVVRKKCISRFYTQTGRGIQNPVLGTIVDSEATRPEWYDFYLISQNARQGTVNPTYYNVLLDENGLKPDHMQRLTYKLCHLYYNWPGVIRVPAPCQYAHKLTFLVGQSIHREPSPELSHKLFYL"

     misc_feature    726..869
                     /gene="piwil4"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    873..1289
                     /gene="piwil4"
                     /note="This domain is named PAZ after the proteins Piwi
                     Argonaut and Zwille; Region: PAZ; smart00949"
                     /db_xref="CDD:198017"
     misc_feature    order(1005..1007,1044..1046,1068..1070,1080..1082,
                     1134..1136,1182..1184,1188..1190)
                     /gene="piwil4"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239211"
     misc_feature    1245..2570
                     /gene="piwil4"
                     /note="PIWI domain, Piwi-like subfamily found in
                     eukaryotes. This domain is found in Piwi and closely
                     related proteins, where it is believed to perform a
                     crucial role in germline cells, via RNA silencing. RNA
                     silencing refers to a group of related...; Region:
                     Piwi_piwi-like_Euk; cd04658"
                     /db_xref="CDD:240016"
     misc_feature    order(1740..1742,1752..1754,1788..1799,1806..1808,
                     1836..1838,1845..1847,1857..1859,1869..1871)
                     /gene="piwil4"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240016"
     misc_feature    order(1932..1934,1938..1940,2142..2144,2544..2546)
                     /gene="piwil4"
                     /note="active site"
                     /db_xref="CDD:240016"
ORIGIN      

cggagcttgagcgagagacgcagttgctggcgctaaagtgaaggaacatcgtatcaggacaaatggctctccaagaaaagccaattgacttctctctgaagaacaaagaaccaggcttggcacgtgattcccctggccaggcttcaaaaatgagcaatcctcgtccagacgcaggcagctccaaagatctacagggaatcggcagtggcaatggcagcggaagagacagcggaattggtaccatctcagctggagagaataggtatgtccttgcagagagaggaggtcggaatggccggaacctacaagattttgggatcaacaccaggcagactatggctcacgttaaggaccacaaaacaggtaccagcggggcgccggtgaaactcatgaccaatctgttcggtttgactttgccgactcagtggaaactgtaccagtaccgtgtggatttctcccctgaatttgcttctaggaaactgaagaccgcactgctctattcacacggggagaccttggggagagccagagcctttcatggggagattctctttctttctcgtaatataggcaagctaaatcatctggaaagtacgactagaaaaggcgaaactgtgaaaataacaatcactctgaccaaccagctgccggccgactcccccgtgtgcatacagttttttggcatcctcttcaagaaggtctttaaaagccttaatatgtaccaagtggggcgcaattactataatcctgctgacccagtgatcattcctcagcacaggttaatgctgtggcctgggtttgtgctctccataatgaaactggaatcccagctggccctgtgcgctgatgtgagccacaaagtcctgcggaatgagactgttcttgactccatgaaagctctgtactccaaagtccccaaggagagattcacagacatatgtgagaaagagctgcttgggcttgttgtgttaaccagatacaacaataaaacctacagagttgacgatattgactggtccaccaaaccgacagattcctttccaaggaaggatggctcagagattacgtacacagactactatagtcagcaatacagcataaacatctccgacctacagcaacccatgttggtgagcacactaaaagctaagaagagtgacaatcccgatgcccctcgtgccgtccacctcattcctgaactgtgctatctcacaggtttgagcagccgtgcactttcggatttccgtttgatgaaggatctgtcaatggaaacccagttggaccctgagcggaggcagcagaggctgcagagactggcggacagcattcagagaaccccagaagccagtgcagagttggagaaatggggcttgaggatggataagcagatacctgtcacgggccgtattgccccaccagagagaatcttcatgagggacaatgtgtgcacccctggatcccctgctgactgggccagggacaccagaaacgagaaggtgatttgtgttgcgcccttgcagaactggctgatgctctttagctaccgtaatgctgacgttgctgagaatctcctgcagtgcctgatgaaagtcgcttctggaatgggattccatatggacatgccaagaatagtgcaggtggaagacaatccggtttcattcctacgcaccctgcagaaaaacgtaacgccagatcttcagctggtgttttgtgtgattcccaccaatcagaaagacagctatgactccatcaagaagttcctgagcctggaccagcctgtccccagccaatgtgtgcagaaccgcactctgggcaggcagcaaatgatgatgagcgttgccaacaagatcgccatgcagatgatctgtaagctcggaggggagctgtgggccgtggagatccctctgaaagccctgatggtggttggaatagacatcaacaaagatgcagtaaacaagtccatgtccgtcgttggatttgtagccagtaccaacgccaggcttactaggtggcactctcgctgcattattcagaaaacaacatcggattttgccgattgcctgaaaatgtgcatgaaaggagccatacagaattggcaaagatgcaacaatgacctcccgaaccgtatagttgtgtacagggacggcgtaggagacgggcagctgaagatggtggtggactatgagattcctcagcttctcagtagcttcagagagaccaagacaagctactgccccagactgtcggtcattgtggtgcggaagaagtgcatctctagattctacacacagaccggcaggggcatccagaacccggtgttggggacaatcgtggactccgaggccacaagacccgaatggtacgatttctacctgatcagccaaaatgcacgacaaggaaccgtgaatccaacctactataatgtgctgctagatgagaacggcctgaaaccggaccatatgcagagactgacctacaaactgtgccatctctattacaattggcccggagtgatccgagtgccagcgccctgccagtacgcacacaaactcaccttccttgtcggccagagtatccacagggagccgagccctgagctctcccacaagctgttctacctgtgagaaattcagatatatcttcggtcattagtgagggaagtctggcctttccttatggatattttattgtattcttccttgttaatatagagaatttatatagtatttataggttaccttagagctggcctacaggcctataggttaccttagagctggcctacaggcctataggttaccttagagctggcctacaggcctatgggttaccttagagctggcctacatgcctatgggttaccttagagctggcctacatgcctatgggttaccttagagctggcctacatgcctatgggttaccttagagctggcctacaggcctatgggttaccttagagctggcctacaggcctatgggttaccttagagctggcctacaggcctatgggttaccttagagctggcctaaaggaatatagcaggctatttttttttatttttttatttttttttatttatttgtatttataagaaaaccgtagctgaaataagtgattaatggtcaagtgaatgtacaatgtacaaaagccttattattatggtataatggccgccttatactctttttactcttttgttgttttaacatttggaataaaagtgatgttttgatgagt

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