LOCUS       NM_001112999            2968 bp    mRNA    linear   VRT 03-APR-2024
DEFINITION  Xenopus tropicalis piwi-like RNA-mediated gene silencing 2
            (piwil2), mRNA.
ACCESSION   NM_001112999
VERSION     NM_001112999.1
KEYWORDS    RefSeq.
SOURCE      Xenopus tropicalis (tropical clawed frog)
  ORGANISM  Xenopus tropicalis
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae;
            Xenopus; Silurana.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Lau,N.C., Ohsumi,T., Borowsky,M., Kingston,R.E. and Blower,M.D.
  TITLE     Systematic and single cell analysis of Xenopus Piwi-interacting
            RNAs and Xiwi
  JOURNAL   EMBO J 28 (19), 2945-2958 (2009)
   PUBMED   19713941
  REMARK    GeneRIF: Xiwi associates with the meiotic microtubules and with
            translational regulators in an RNA-dependent manner, but Xenopus
            tudor interacts with Xiwi independently of RNA.
            Erratum:[EMBO J. 2009 Nov 4;28(21):3458]
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Klein,S.L., Strausberg,R.L., Wagner,L., Pontius,J., Clifton,S.W.
            and Richardson,P.
  TITLE     Genetic and genomic tools for Xenopus research: The NIH Xenopus
            initiative
  JOURNAL   Dev Dyn 225 (4), 384-391 (2002)
   PUBMED   12454917
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC154090.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC154090.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00028290, SAMD00028291
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Xenopus tropicalis"
                     /mol_type="mRNA"
                     /db_xref="taxon:8364"
                     /chromosome="3"
                     /map="3"
     gene            1..2968
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /note="piwi-like RNA-mediated gene silencing 2"
                     /db_xref="GeneID:100127654"
                     /db_xref="Xenbase:XB-GENE-982236"
     exon            1..75
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /inference="alignment:Splign:2.1.0"
     exon            76..247
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /inference="alignment:Splign:2.1.0"
     CDS             77..2926
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /note="piwi-like 2"
                     /codon_start=1
                     /product="piwi-like protein 2"
                     /protein_id="NP_001106470.1"
                     /db_xref="GeneID:100127654"
                     /db_xref="Xenbase:XB-GENE-982236"
                     /translation="

MDPTRPPFRGSPFHTPLGVRPPVLETKEEGPHGRAVLLPRGRALLGASAPSSDTTQRDPSDSRNVLPALFRGMGIETKPSGIPGRGGPVFGRGFLSTMSSGDGPDQPLSEPSIRPSLSTRVQQASDFSTERVALGRARFPIPPVSMEKPHVPTGRGLLFPSVLPTLTSDPVPKESPIATLQIEKEEKWEPLPKKGSKGSPCQLGLNLIKINFQNEAVYQYHVTFTPIVECRSMRFGMMKDHRSVTGPVTAFDGSILYLPVKLAQTVELESERRTDGQKIKITIQMTKILDPSSDLCLPFYNVVMRRVFKILDLKLVGRNFYDPASSTVLQQYRLQVWPGYAANIRKTDGGLFLLVDITHKIIRSDSVLDIMNILYQQSPENFQDEVTKQLVGSIVITRYNNRTYRIDDIEWNMSPKDSFSMSDGSKISFIDYYSKNYGITVKELDQPLLLHRPSERKAPKGKALDIVLLLPELAFMTGIPEKMRKDFRAMKDLTQQIHLSPKQHHISLGKLLKRIESSADAKNELQRWGLFLDTDIHMTTGRILPIEKINLRNNSFPAGEDLNWNREVTREACRSSVHLLYWAMIYPKRASAQAQELSSMLERIGGPIGIRVNHPNCVELRDDRVETYARSIKSLLEGEGKVQLLVCLISGTRDDLYGAIKKLCCVQNPVPSQVINTRTISQPQKLRSIAQKILLQINCKLGGELWGVDIPLKSVMVIGMDVYHDPSRGMRSVLGFVASINSCLTAWYSRVVFQLPNQEIMDSLKLCLVAALQKFFEVNHSLPEKIVVYRDGVSDGQLNTVENYEIPQLQTCFQTFDNYNPRMVVIVVQKRVSTNLYSTATGQFLTPQPGTVIDHTVTNRKWIDFFLMSHHVRQGCGIPTHYICVMNTANLGPDHLQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGQFLHHEPSIKLCDKLFFL"

     misc_feature    77..451
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /note="propagated from UniProtKB/Swiss-Prot (A8KBF3.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    <83..613
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /note="large tegument protein UL36; Provisional; Region:
                     PHA03247"
                     /db_xref="CDD:223021"
     misc_feature    1022..1165
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    1169..1582
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /note="This domain is named PAZ after the proteins Piwi
                     Argonaut and Zwille; Region: PAZ; smart00949"
                     /db_xref="CDD:198017"
     misc_feature    order(1298..1300,1337..1339,1361..1363,1373..1375,
                     1427..1429,1472..1474,1481..1483)
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239211"
     misc_feature    1538..2872
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /note="PIWI domain, Piwi-like subfamily found in
                     eukaryotes. This domain is found in Piwi and closely
                     related proteins, where it is believed to perform a
                     crucial role in germline cells, via RNA silencing. RNA
                     silencing refers to a group of related...; Region:
                     Piwi_piwi-like_Euk; cd04658"
                     /db_xref="CDD:240016"
     misc_feature    order(2045..2047,2057..2059,2093..2104,2111..2113,
                     2141..2143,2150..2152,2162..2164,2174..2176)
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240016"
     misc_feature    order(2237..2239,2243..2245,2447..2449,2846..2848)
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /note="active site"
                     /db_xref="CDD:240016"
     exon            248..347
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /inference="alignment:Splign:2.1.0"
     exon            348..489
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /inference="alignment:Splign:2.1.0"
     exon            490..639
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /inference="alignment:Splign:2.1.0"
     exon            640..750
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /inference="alignment:Splign:2.1.0"
     exon            751..868
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /inference="alignment:Splign:2.1.0"
     exon            869..993
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1074
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /inference="alignment:Splign:2.1.0"
     exon            1075..1188
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /inference="alignment:Splign:2.1.0"
     exon            1189..1377
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..1462
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /inference="alignment:Splign:2.1.0"
     exon            1463..1549
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /inference="alignment:Splign:2.1.0"
     exon            1550..1690
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /inference="alignment:Splign:2.1.0"
     exon            1691..1804
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /inference="alignment:Splign:2.1.0"
     exon            1805..1993
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /inference="alignment:Splign:2.1.0"
     exon            1994..2095
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /inference="alignment:Splign:2.1.0"
     exon            2096..2212
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /inference="alignment:Splign:2.1.0"
     exon            2213..2301
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /inference="alignment:Splign:2.1.0"
     exon            2302..2407
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /inference="alignment:Splign:2.1.0"
     exon            2408..2661
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /inference="alignment:Splign:2.1.0"
     exon            2662..2769
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /inference="alignment:Splign:2.1.0"
     exon            2770..2962
                     /gene="piwil2"
                     /gene_synonym="ct80; hili; mili; piwil1l; xili"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

ccgcccaaaagtgagtgaacctgagcagtgggattaccctgagtgtgagagagagtcgagtcgcgcctcgttgagaatggatcctaccagacctccattcaggggttccccctttcatacaccattgggagtgcgtcctccagttctagaaactaaggaagaagggcctcatggaagagcagtcctattaccacggggccgtgcattattgggtgcatcagcaccttcttctgatactacccaaagagatccatctgactctaggaatgttctccctgctctatttcgtggtatgggaattgaaacaaagcccagtgggattcctggccgtggtgggccagtctttggcagggggtttctttctactatgtcttcaggagatggccctgatcagccactttcagaaccctctataagaccctccctctcaacccgtgttcagcaagcaagtgattttagcacagagagggttgcacttgggagagccaggtttccaatacctccggtgagtatggaaaagccacatgtacccactggaaggggtttgttatttccctctgttctacccactttgacatctgacccagtacctaaagaaagccctatcgctacacttcaaattgagaaggaagagaaatgggagcctttaccaaagaaaggcagcaaaggtagcccctgtcagcttggactgaacctcattaaaattaacttccagaatgaagccgtatatcagtaccatgtcacatttactcccatagtggagtgccgtagtatgcgttttggtatgatgaaggaccaccgctctgtgacaggaccagtaactgcattcgacggttctatactttaccttcctgtcaaacttgcacagacagttgagctggagagtgaacgccggacagatggacaaaaaataaagataaccatacagatgacaaagattctggatcccagttcagacttgtgccttcctttttataatgtagtcatgaggagggtgttcaaaattctggacctgaaacttgttgggagaaatttttatgacccagcaagttcaacagtgctgcagcagtacagactgcaggtgtggccaggctatgctgcaaatatacgcaaaactgacggtgggctatttcttttggttgacatcacccacaagattatccgcagtgattctgtgctggacatcatgaacattctatatcagcagagccctgagaactttcaggatgaggttaccaagcagctggttggcagcattgtgatcaccagatacaacaaccgtacctaccgaatagatgacattgaatggaacatgagccccaaggactccttctctatgtcagatggaagcaaaatcagtttcattgattattacagcaagaactatggcataactgttaaggagctggaccaacccttgctgcttcacagacctagtgaaaggaaggcaccaaagggcaaggcacttgatattgtcctcctcttaccagaacttgcttttatgactggcatccctgaaaaaatgagaaaagactttcgggccatgaaggatctgacacagcagattcatttaagcccaaaacagcatcatatctctttggggaaattgctaaaacgtattgaaagcagtgcggatgcaaagaatgaactgcagcgttggggcttattcctagacactgatattcacatgaccactggtaggattttaccaatagagaaaataaatctgcgtaataactcatttcctgcaggagaagatctcaattggaacagggaagtgacacgtgaggcatgccgttcctcagtgcatcttctttattgggcaatgatatatcctaaacgggcttcagcacaggcacaagaattgtctagtatgctggagaggattggaggacctattggaatacgagtaaaccatcctaactgtgtggagctccgggatgaccgtgtcgaaacgtacgcgcgcagcatcaaatcactgttagagggtgagggcaaggttcagctactagtgtgtcttatatctgggaccagagatgacctgtacggtgctattaagaaattgtgttgtgtgcagaacccagttccatcacaggtgattaataccagaaccatctcccagccccagaagctcaggagcattgcacagaaaattctgcttcagattaactgcaagctgggaggggaactgtggggagtagacatccctctgaaaagtgtgatggtgattgggatggatgtttatcacgaccccagcagaggcatgcgctctgtattaggctttgttgctagcatcaacagctgcctgactgcttggtattctcgtgtggtctttcagcttcctaaccaggagatcatggatagcctaaagctttgccttgtggcagccctgcagaaattctttgaggtgaatcacagtcttccagagaagattgtggtgtatcgagatggcgtatctgatgggcaacttaacacggtggaaaactatgagatcccacagcttcaaacttgcttccagacatttgataactataatccacgtatggtggttattgtagtacagaaaagggttagcactaatctgtactctaccgccactgggcagtttcttacccctcaacctgggactgttatagaccatacagtcaccaaccgcaagtggatagatttcttcctgatgtctcatcatgtgagacaaggctgcgggatccccacccactacatttgtgtcatgaatacagcaaatttgggtcctgaccatttacagaggttgaccttcaagctttgccacatgtactggaactggcctgggaccatccgagtgccagctccatgtaaatacgcacacaagctggccttcctttccggtcagtttctgcaccacgagccttccataaagctgtgtgacaagcttttcttcctttgaaacagagtcacaaatggagcccaatacaaaaaaaaaaaaaaa

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