LOCUS       NM_001180574            1920 bp    mRNA    linear   PLN 17-DEC-2024
DEFINITION  Saccharomyces cerevisiae S288C E3 ubiquitin-protein ligase HEL2
            (HEL2), partial mRNA.
ACCESSION   NM_001180574
VERSION     NM_001180574.3
DBLINK      BioProject: PRJNA128
KEYWORDS    RefSeq.
SOURCE      Saccharomyces cerevisiae S288C
  ORGANISM  Saccharomyces cerevisiae S288C
            Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
            Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
            Saccharomyces.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
            Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
            Weng,S. and Cherry,J.M.
  TITLE     New data and collaborations at the Saccharomyces Genome Database:
            updated reference genome, alleles, and the Alliance of Genome
            Resources
  JOURNAL   Genetics 220 (4) (2022)
   PUBMED   34897464
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Jacq,C., Alt-Morbe,J., Andre,B., Arnold,W., Bahr,A., Ballesta,J.P.,
            Bargues,M., Baron,L., Becker,A., Biteau,N., Blocker,H., Blugeon,C.,
            Boskovic,J., Brandt,P., Bruckner,M., Buitrago,M.J., Coster,F.,
            Delaveau,T., del Rey,F., Dujon,B., Eide,L.G.,
            Garcia-Cantalejo,J.M., Goffeau,A., Gomez-Peris,A., Zaccaria,P. et
            al.
  TITLE     The nucleotide sequence of Saccharomyces cerevisiae chromosome IV
  JOURNAL   Nature 387 (6632 SUPPL), 75-78 (1997)
   PUBMED   9169867
REFERENCE   3  (bases 1 to 1920)
  AUTHORS   Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
            Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
            Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
            Oliver,S.G.
  TITLE     Life with 6000 genes
  JOURNAL   Science 274 (5287), 546 (1996)
   PUBMED   8849441
REFERENCE   4  (bases 1 to 1920)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2024) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   5  (bases 1 to 1920)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2015) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Protein update by submitter
REFERENCE   6  (bases 1 to 1920)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2013) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Protein update by submitter
REFERENCE   7  (bases 1 to 1920)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2012) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Protein update by submitter
REFERENCE   8  (bases 1 to 1920)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2011) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Sequence update by submitter
REFERENCE   9  (bases 1 to 1920)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2009) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
COMMENT     REVIEWED REFSEQ: This record has been curated by SGD. This record
            is derived from an annotated genomic sequence (NC_001136).
            
            On Jul 30, 2012 this sequence version replaced NM_001180574.2.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider :: SGD
            Annotation Status   :: Full Annotation
            Annotation Version  :: R64-4-1
            URL                 :: http://www.yeastgenome.org/
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on both ends.
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Saccharomyces cerevisiae S288C"
                     /mol_type="mRNA"
                     /strain="S288C"
                     /db_xref="taxon:559292"
                     /chromosome="IV"
     gene            <1..>1920
                     /gene="HEL2"
                     /locus_tag="YDR266C"
                     /gene_synonym="RQT1"
                     /db_xref="GeneID:851859"
     CDS             1..1920
                     /gene="HEL2"
                     /locus_tag="YDR266C"
                     /gene_synonym="RQT1"
                     /EC_number="2.3.2.27"
                     /experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
                     [PMID:14562095]"
                     /experiment="EXISTENCE:direct assay:GO:0022626 cytosolic
                     ribosome
                     [PMID:33338396|PMID:32615089|PMID:28757607|PMID:30609991|P
                     MID:30893611]"
                     /experiment="EXISTENCE:direct assay:GO:0055106
                     ubiquitin-protein transferase regulator activity
                     [PMID:32615089]"
                     /experiment="EXISTENCE:direct assay:GO:0061630 ubiquitin
                     protein ligase activity
                     [PMID:30609991|PMID:28757607|PMID:30893611|PMID:22570702]"
                     /experiment="EXISTENCE:direct assay:GO:0070534 protein
                     K63-linked ubiquitination
                     [PMID:28757607|PMID:30609991|PMID:30893611]"
                     /experiment="EXISTENCE:direct assay:GO:0070651
                     nonfunctional rRNA decay [PMID:30893611]"
                     /experiment="EXISTENCE:direct assay:GO:0070966
                     nuclear-transcribed mRNA catabolic process, no-go decay
                     [PMID:30609991]"
                     /experiment="EXISTENCE:direct assay:GO:0072344 rescue of
                     stalled ribosome
                     [PMID:33338396|PMID:32615089|PMID:30609991]"
                     /experiment="EXISTENCE:direct assay:GO:0170011 stalled
                     ribosome sensor activity [PMID:32615089|PMID:33338396]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0010629
                     negative regulation of gene expression [PMID:31532761]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0036205 histone
                     catabolic process [PMID:22570702]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0061157 mRNA
                     destabilization [PMID:31532761]"
                     /note="RING finger ubiquitin ligase (E3); subunit of
                     ribosome-associated quality control trigger complex (RQT);
                     involved in ubiquitination and degradation of excess
                     histones; interacts with Ubc4p and Rad53p; required to
                     trigger ribosome-associated quality control (RQC) and
                     canonical no-go decay; ubiquitinates Rps20 and Rps3; binds
                     to ribosomal RNA, mRNA and tRNA"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase HEL2"
                     /protein_id="NP_010552.3"
                     /db_xref="GeneID:851859"
                     /db_xref="SGD:S000002674"
                     /translation="

MSESVKENVTPTRNFRRTQGPQNNTKPHNDRKNFRRKQKKNNLSAEPNLTTSSADDTDEENELCVICARKLTYVSLTPCHHKTCHICGFRQRALYNKKSCLICRTENEEVMFTDRIDGDISDKYNFCEKNEKYGINFTSEEVATETLNLLKFFCPLSKDEQVCDFGSFKKYNEHLKSEHNRMICLICATHKHAFPCELEIFTQNQLRNHQTKGNSEGFKGHPMCAFCSGKRFYSDDELYIHMRNQHEKCHICDKMNPASPQYFKDYNQLFDHFKHSHYVCTVQTCLDNKFVVFKDELELQAHILQEHGNILKGKPKFFQSELSTFISAPSRVIRERDDYDLPSISSLPGSSSGSRTDVRSASSPEESRLRLAERAKYYLENSKEDFNKFSSYNEDYSKGRLSAEKLLESYKLLFTKPNADVYLLIHNLAETFPKNSSKYNNLNAIYEQREQTLARQTSLPSLSSDSSLSMSIGRGHWGGTNDGGSAGAALGVRNIKNLPTLKSPSASYDPFATTVKKNTLRPVQNIKRTTPQSVSYRTSTNTVAFSPTYLESKKGSSSTSLNNSKDKLKSLNLPQLPPPKPKVQIPGLNRPQIADPKQWGKKSSTQDTNVHDNLRELNTTSGGNKKKGKQKQLLFHIGV"

     misc_feature    1..1521
                     /gene="HEL2"
                     /locus_tag="YDR266C"
                     /gene_synonym="RQT1"
                     /note="Uncharacterized conserved protein, contains RING
                     Zn-finger [General function prediction only]; Region:
                     COG5236"
                     /db_xref="CDD:227561"
ORIGIN      

atgagcgaatcagtgaaagaaaacgttactccgacacgtaacttccgtcgtactcaagggccccaaaataatacaaaacctcacaacgacaggaagaatttcagaagaaagcaaaagaaaaataacctttctgctgaacccaacctcaccacttcaagcgcagatgatactgatgaagaaaatgaattatgtgtaatttgtgcgcgcaagttaacatacgtatctctaacaccatgccatcataagacatgccatatctgtggtttcagacaacgagctttgtacaacaagaaaagctgtttgatttgcagaacggagaatgaagaagtcatgtttaccgatcgcatagatggtgatatttcagataaatataacttttgcgaaaaaaatgaaaaatatggaataaatttcactagcgaagaggttgctaccgaaacactaaatcttttaaaatttttttgtcccctatcgaaggatgaacaagtttgtgactttggtagtttcaagaaatataatgaacacttgaaatctgaacataacagaatgatctgtttaatatgtgcaacccataagcatgcattcccttgtgagcttgaaatattcacccaaaatcaattacgcaaccatcaaactaagggtaattcagaaggattcaaaggccaccctatgtgtgccttctgttccggaaaacgattttattcagacgacgaattgtacattcacatgagaaatcagcatgaaaagtgccatatttgtgataagatgaaccccgcttctcctcaatactttaaggattataatcagctttttgaccactttaaacattcgcattacgtttgcaccgtccaaacttgcttagataacaagtttgtcgtatttaaagacgagttggagttacaagcccacattttgcaggagcatggtaatatcctgaagggaaagccaaagtttttccagtcagaactttctacttttatctcggctccatctagagtgatcagggaaagagatgattacgaccttccatctatatcttccctaccgggttcatcatccggctctagaactgatgtgagatctgctagttctcccgaggaaagtagactaagattggcagaaagagcaaaatactatctggaaaattccaaagaagacttcaataagttttcttcttataacgaagattattctaaaggtcgcttaagtgctgaaaaattattggaatcatataaattgttattcacaaaaccaaatgcggatgtatatttattgattcataacttagctgaaacctttcccaaaaactcctccaaatacaataatctgaacgccatatatgaacaaagagagcaaacccttgcacgacaaacttcactaccttctttatctagtgattcttccctttcaatgtcaataggcaggggccattggggtggaacaaacgatgggggcagcgctggtgcagcattaggcgttaggaatatcaaaaatcttccaaccctgaaaagtccttcagcgtcgtatgatccctttgcaaccaccgtaaaaaaaaacactctgagacccgtgcaaaacattaaaagaactacacctcaatcagttagttatcgaacttccactaatacagtcgccttctctccaacctatttggaaagtaaaaagggttcctcttctacttctctaaataatagcaaagataaattgaaaagtctcaacctacctcaactacctccaccaaagccaaaggtgcaaattccaggtctgaacagaccccaaattgctgatcctaagcagtggggtaaaaagtcaagcacacaagatactaacgtgcacgataatttgagggaattgaacacaactagtggaggaaataaaaagaaaggcaaacaaaagcagctgttattccacattggtgtatag

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