LOCUS       XM_039089632            4659 bp    mRNA    linear   ROD 22-FEB-2024
DEFINITION  PREDICTED: Rattus norvegicus piwi-like RNA-mediated gene silencing
            1 (Piwil1), transcript variant X2, mRNA.
ACCESSION   XM_039089632
VERSION     XM_039089632.2
DBLINK      BioProject: PRJNA1074393
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_086030) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Feb 22, 2024 this sequence version replaced XM_039089632.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_036323735.1-RS_2024_02
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.2
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 02/09/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /bio_material="RGD 61498"
                     /db_xref="taxon:10116"
                     /chromosome="12"
                     /sex="male"
                     /tissue_type="kidney, spleen, liver"
                     /geo_loc_name="USA: Wisconsin, Milwaukee"
                     /lat_lon="43.05 N 88.04 W"
                     /collected_by="Rebecca Schilling, Melinda Dwinell"
     gene            1..4659
                     /gene="Piwil1"
                     /note="piwi-like RNA-mediated gene silencing 1; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 59 long SRA reads"
                     /db_xref="GeneID:363912"
                     /db_xref="RGD:1306980"
     CDS             802..3393
                     /gene="Piwil1"
                     /codon_start=1
                     /product="piwi-like protein 1 isoform X1"
                     /protein_id="XP_038945560.1"
                     /db_xref="GeneID:363912"
                     /db_xref="RGD:1306980"
                     /translation="

MTGRARARARGRARGQETVQHVGAAAQSQQPGYIPPRPQQSPTEGDLVGRGRQRGMVVGATSKSQELQISAGFQELSLAERGGRRRDFHDLGVNTRQNLDHVKESKTGSSGIIVKLSTNHFRLTSRPQWALYQYHIDYNPLMEARRLRSALLFQHEDLIGRCHAFDGTILFLPKRLQHKVTEVFSQTRNGEHVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPSDPIDIPNHRLVIWPGFTTSILQYENNIMLCTDVSHKVLRSETVLDFMFNLYQQTEEHKFQEQVSKELIGLIVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVSQPKRRRGPGGTLPGPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKDDNVQRELRDWGLSFDSNLLSFSGRILQSEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGIQMKKAIMIEVDDRTEAYLRALQQKVTSDTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDMPLKLAMIVGIDCYHDTTAGRRSIAGFVASINEGMTRWFSRCVFQDRGQELVDGLKVCLQAALRAWSGCNEYMPSRVIVYRDGVGDGQLKTLVNYEVPQFLDCLKSVGRGYNPRLTVIVVKKRVNARFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDSSGLKPDHIQRLTYKLCHVYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPNLSLSNRLYYL"

     misc_feature    847..1125
                     /gene="Piwil1"
                     /note="GAGE protein; Region: GAGE; pfam05831"
                     /db_xref="CDD:461753"
     misc_feature    1489..1632
                     /gene="Piwil1"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    1636..2052
                     /gene="Piwil1"
                     /note="This domain is named PAZ after the proteins Piwi
                     Argonaut and Zwille; Region: PAZ; smart00949"
                     /db_xref="CDD:198017"
     misc_feature    order(1768..1770,1807..1809,1831..1833,1843..1845,
                     1897..1899,1945..1947,1951..1953)
                     /gene="Piwil1"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239211"
     misc_feature    2008..3339
                     /gene="Piwil1"
                     /note="PIWI domain, Piwi-like subfamily found in
                     eukaryotes. This domain is found in Piwi and closely
                     related proteins, where it is believed to perform a
                     crucial role in germline cells, via RNA silencing. RNA
                     silencing refers to a group of related...; Region:
                     Piwi_piwi-like_Euk; cd04658"
                     /db_xref="CDD:240016"
     misc_feature    order(2509..2511,2521..2523,2557..2568,2575..2577,
                     2605..2607,2614..2616,2626..2628,2638..2640)
                     /gene="Piwil1"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240016"
     misc_feature    order(2701..2703,2707..2709,2911..2913,3313..3315)
                     /gene="Piwil1"
                     /note="active site"
                     /db_xref="CDD:240016"
     polyA_site      4659
                     /gene="Piwil1"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

aacccagaggtctgcggaggtagctcccggaggcctgtgcggaggggcggagggatctggcagttggggctgttagcaggtgcccgcgggaggtgcagcagcagtgcgaggtggtggcagccctgcgcccgcgagaagcctgctgcagcgacccggcgtgaggagcctcaggggctgcggacctaagcgtgctgagcacggggccaagataggaccagacgctgctggaccacgacgatcagggtctggaagcaagctatgcccacctgtgccttgacctcaacagacctgtacccaagcccaggcttggtcccgacctctctctatgctcgaactttatctatacctctttctctgtctggaacttgtcctctcctcgttgttctgggcctatagcccactctaacctgtttcctttctctccttgtgcccaagcctggcccacatccctcctcctgcctgtcctcacctctctattgttcctggacctcttccacacgtatccctgtgtctgagtctggtccccggttcccccagtccccacctctccatgccaaagcacataatagcatctctctaactatatccccagtctgctacagcctccagctctatccgtatcctcactggttttccacctgcctccctatgctttcacatccgaatcccagtcgttatcacagctgtgttacaatgcagagagacctggctcacttacatggcgcgaccacaccggcatccagcctgcgacacacgtaggattggaaggaggccaagctgaacacctgagctgactggaaaatgactggacgagcccgagcccgggcccgtggcagggcccgaggtcaggagacggtgcagcatgttggggctgctgcacagagccagcaacctgggtacatcccgccgagaccccagcagtcgcccacagagggggacttggttggccgaggacgacagaggggaatggtagtcggagccacgtccaagtcacaagaactgcagatctcagccgggtttcaggagctgtcactggcagagagaggagggcgtcgccgagacttccatgaccttggtgtgaacaccagacagaaccttgaccatgtcaaagagtcaaagacaggctcctctggcatcattgtgaagctgagcaccaaccacttccggctgacctcgcgcccacagtgggccctgtaccagtaccacattgactataaccccctgatggaggcccgcaggctgcgctctgccctgctcttccagcacgaagacctcattggaaggtgtcatgctttcgacgggacaatattgtttttacctaagagactacagcacaaggtcacggaagtattcagtcagactcggaatggggaacacgtgcggattaccatcaccctgaccaacgagctgccgcccacctcacccacctgcctgcagttctataacatcatcttcaggaggctcttgaaaatcatgaatttgcaacaaattggacggaattattacaacccaagtgacccgattgatattccaaatcacaggttggtgatctggcccggcttcaccacctccatacttcaatatgagaacaacatcatgctttgcacagacgtcagccacaaggtcctccgcagcgagaccgttctagacttcatgttcaatctgtaccagcagacggaggagcacaagttccaggagcaagtgtccaaggagctcataggcctcatcgtcctcaccaagtacaacaacaagacctacagggtggatgacatcgactgggaccagaatccaaagagcaccttcaagaaggcagatggctcggaggtcagcttcctggagtactacaggaagcaatacaaccaggagatcacagacttgaagcagccagtgctagtgagccagcccaagcggaggagaggccctggcggcaccctgcctggcccagccatgctcatcccagagctctgctacctcacaggcctgactgataaaatgcgcaatgatttcaacgtgatgaaggacctggcggtgcacacacggctgacccctgagcagcggcagcgggaggtgggccgcctcatcgactacatccacaaggatgacaatgtgcagagagagcttcgagactggggcctaagcttcgattcgaacttgctgtccttctcaggaagaatcttacaatccgagaagatccaccagggtggaaagacgtttgattacaacccacaatttgcagactggtccaaagaaacgagaggcgcgccgctgatcagcgtgaagccgttggataactggctgctgatctatacccgcaggaattatgaagcggccaactcgctgatacagaacctgttcaaagtgactccggccatgggcatccagatgaaaaaggccatcatgatcgaggtggacgacagaacagaagcttatctgagagccttgcagcagaaggtgacatcagacactcagatagttgtctgtctcttgtcaagtaatcggaaggacaagtatgatgccatcaagaagtatttgtgcacagactgccccaccccaagtcagtgtgtggtggcccggacgctgggcaagcagcaaacagtcatggccattgccaccaagatcgccctgcagatgaactgcaagatgggaggcgagctctggcgggtggacatgcctctgaagctggcaatgatcgtgggcattgactgttaccatgacaccacagctgggcggaggtccatcgcaggattcgttgccagcatcaatgaagggatgacccgttggttctcccgctgcgtctttcaggaccgtggacaggagctggtagacggtctgaaggtgtgcttgcaagctgccctgagggcttggagtggctgcaatgaatatatgcccagccgagtcattgtgtaccgagacggtgtgggggacgggcagctgaagaccctggtcaattatgaggtcccacagttcctagactgcctcaagtcagtcgggagaggttacaacccaagactgaccgtgatcgtagtgaagaagcgagtcaatgccaggttttttgctcagtctgggggaagacttcagaaccctcttccggggacagtcatcgatgtggaagtcaccagaccagagtggtatgactttttcatcgtgagccaggcagtgagaagtgggagtgtgtccccaacacactacaatgtcatctacgacagcagtggcctgaagcccgaccacatccagcggctgacgtacaagctgtgtcacgtgtactacaattggcctggagtcatccgagtccctgcaccttgccagtatgcacacaagctggccttcctcgtgggccagagcatccacagagagccgaacctctccctgtccaaccgtctctactacctctaaccagcagcgagggcctacatgctggtttcctccagagctctgccaacctttagttctgtttctcatatgatgttgttctagcgggatgctgggggtgggggttgggggccgcggtttcgttttttttttttttttttagttggtttgtagtggttatagagttaactaaggttcactgtttgctttgtttctcatagagcgttgtgacaatttgcttggcagagtggtggcagggggccgtggtaccacagatagactttcctaaagcagccgagtcggcaggttctcggaggtgctcctgggagcacgtcctactgtacgctgcgctctctctctctctctctctctctctctctctctctctctctctctctctgcatgtttgaatttaaaggaggaaaagacgcaaggcaaaaatacccagagcatcctaggctgatgctatgtgggactctgaccctctgacacctcagatcctaaagataaaaaggccgggggtgcctctctgaatttcttttaagagttaggttatagggaatgaaaagaattgttgagggttggggatttagctcagtggtagagcgcttgcctagcaagcgcaaggccctgggttcggtccccagctccgaaaaaaaagaaaaaagaaaaaaaaataataattgttgaaagttttaaacttttctccatgaaagtgttttaagactgatgaggccggtggtggaacgagtgttgttttctttcgcaattttctcaccgagtgaaattagcgaccctcactttatttaaacagtttttagttttcgaaggcccgtgttttctttgtttttaaactgcagaagaccactctgggataggattccactcccgaaagtaaggtgtcgggcgtggtggcacacacctgtgatctcagcacttgggaggtgaagcccggggaaccccaagttcccggtcagcctaggctacatagtgagaccttgtcttaacaaacaggaaaataaaagaaaaatggagcagttatgagttatgggtgtcagtttctgcaggagactctgggagtaagagctgcccctgtccccagaggcagctggtgggccaattcaggtgccaggcaaggtatggccacatatgtttgtctggctctttggcagtgaaggaggcttggagttatgtcacgagagcagcttgaagtggctacagtgtgatgagttttacacaatggcagtgtgtgcccccggcacagctcaataaagtttctgtctcacttta

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