LOCUS       XM_008765980            3265 bp    mRNA    linear   ROD 22-FEB-2024
DEFINITION  PREDICTED: Rattus norvegicus piwi-like RNA-mediated gene silencing
            4 (Piwil4), transcript variant X1, mRNA.
ACCESSION   XM_008765980
VERSION     XM_008765980.4
DBLINK      BioProject: PRJNA1074393
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_086026) annotated using gene prediction method: Gnomon,
            supported by mRNA and EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Feb 22, 2024 this sequence version replaced XM_008765980.3.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_036323735.1-RS_2024_02
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.2
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 02/09/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /bio_material="RGD 61498"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="kidney, spleen, liver"
                     /geo_loc_name="USA: Wisconsin, Milwaukee"
                     /lat_lon="43.05 N 88.04 W"
                     /collected_by="Rebecca Schilling, Melinda Dwinell"
     gene            1..3265
                     /gene="Piwil4"
                     /note="piwi-like RNA-mediated gene silencing 4; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 1 mRNA, 4 ESTs, 9 long SRA reads, 4 Proteins"
                     /db_xref="GeneID:689972"
                     /db_xref="RGD:1311015"
     CDS             108..2654
                     /gene="Piwil4"
                     /codon_start=1
                     /product="piwi-like protein 4 isoform X1"
                     /protein_id="XP_008764202.1"
                     /db_xref="GeneID:689972"
                     /db_xref="RGD:1311015"
                     /translation="

MSGRARVRARGITTGHSAREVERAPRDLMVTSVSPGDNEASSRTSVISQPHELGVSSGDGRRTLVEKRGNGRQDFVDLGVCTREKLTHVKDSKTGSSGIPVQLATNLFHLDLPQDWQLYQYHVTYSPDLASRRLRTALLYNHSILSDKAKAFDGTSLFLSEKLDHKVTELTSETQRGETVKITLTLTSELFPNSPMCIQFFNVIFRKIFKKLSMYQIGRNYYKPSEPVEIPQYKLSLWPGFAISVSRFESKLLFNADVNYKVLRNETVLEFMTDLCLRTDMSCFTETCHKQLVGLIVLTRYNNKTYRIDDIDWSVKPTHAFQKRDGSETTYVDYYKQQYDITLSDLNQPVLVSLLKRRRNDNSEPQMVHLIPELCFLTGLTSQATSDFRLMKAVAEETRLSPMGRQQQLARLADDIQRNQVTRFELETWGLHFGSQLSLTGRVVPSEKILLQDHTCQPAFAADWSKDMRSCKVLSSEPLNTWLIVCCSRAEHLIESFLSCLRRVGGSTGFSVGHPKIIKVDENPAAFLRAIQLHVNPDVQLVMCILPSNQKNYYDSIKRYLSSDCPVPSQCVLTRTLNKQGMMLSVATKIAMQMTCKLGGELWAVEIPLKSLMVVGIDICRDALSKDVAVVGFVASINSRITRWFSRCVLQRTAADIADCLKVCMTGALNKWYKHNHDLPARIIAYRDGVGNGQLKAVLEYEVPQLLSSVTECGSDARSCRLSVVVVRKRCLLRLFAESGHTLQNPPLGTVVDSEATRPEWYDFYLTSQTANRGTVSPTYYNVIYDDNALKPDHMQRLTFKLCHLYYNWQGVISVPAPCQYAHKLTFLVAQSIHKEPSLELANKLFYL"

     misc_feature    900..1250
                     /gene="Piwil4"
                     /note="PAZ domain, Piwi_like subfamily. In multi-cellular
                     organisms, the Piwi protein appears to be essential for
                     the maintenance of germline stem cells. In the Drosophila
                     male germline, Piwi was shown to be involved in the
                     silencing of retrotransposons in the...; Region:
                     PAZ_piwi_like; cd02845"
                     /db_xref="CDD:239211"
     misc_feature    order(1035..1037,1074..1076,1098..1100,1110..1112,
                     1164..1166,1209..1211,1215..1217)
                     /gene="Piwil4"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239211"
     misc_feature    1272..2600
                     /gene="Piwil4"
                     /note="PIWI domain, Piwi-like subfamily found in
                     eukaryotes. This domain is found in Piwi and closely
                     related proteins, where it is believed to perform a
                     crucial role in germline cells, via RNA silencing. RNA
                     silencing refers to a group of related...; Region:
                     Piwi_piwi-like_Euk; cd04658"
                     /db_xref="CDD:240016"
     misc_feature    order(1767..1769,1779..1781,1815..1826,1833..1835,
                     1863..1865,1872..1874,1884..1886,1896..1898)
                     /gene="Piwil4"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240016"
     misc_feature    order(1959..1961,1965..1967,2169..2171,2574..2576)
                     /gene="Piwil4"
                     /note="active site"
                     /db_xref="CDD:240016"
     polyA_site      3265
                     /gene="Piwil4"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

aaccatactcccaatctccgagtcacagcctcaccctctccctgcggaagaggaaatcctcctgtagaggtcctgcagccttggaaagccgctgggaaaccaggaacatgagtggacgggcccgtgtgagggcgcgaggcatcaccactggccacagtgccagggaagtggagcgggccccaagagacctcatggtcacatctgtcagtcccggggacaacgaggccagcagtaggacaagcgtcatttcacagccacatgagcttggcgtctcttctggtgatggtagacgtactctcgtggaaaaaagaggcaatggcagacaggacttcgtcgatttgggtgtctgtaccagggagaagttgactcacgtaaaagattctaaaacaggttctagcggaatacctgtgcagctggctacaaatctcttccatttagatttgccccaggattggcagctataccagtaccatgtcacatacagcccggatttagcctctaggaggctgaggactgctctgctgtataaccacagcatactctccgacaaggcaaaggcattcgacggcaccagccttttcctctcagaaaagctggaccacaaagtcacagaactgacaagtgaaacccaaagaggggagactgtgaagataactctcaccctgaccagtgagctgttcccaaactccccgatgtgcattcagtttttcaatgtcatcttcagaaagatctttaaaaagttgagcatgtaccagattggacggaactactataagccttcagagccagtggagatcccccagtacaaattgtccctgtggcctgggtttgccatttctgtgtctcgctttgaaagcaaactcctgttcaatgctgacgtaaactataaagtcctccgcaatgagactgttctggaattcatgactgatctctgcctcagaaccgacatgtcatgcttcactgagacgtgccacaaacagctagtggggctcattgtccttaccaggtacaacaacaaaacatatcgtatcgatgacattgactggtctgtgaagcccacacatgcctttcagaagcgggatggctcagagaccacgtatgtggattactacaagcagcaatatgatatcaccttatctgacctaaatcagccagtgcttgtcagtctgttgaaaagaaggagaaatgataactccgagcctcagatggtccacctgatcccggagctctgctttctcacgggcctgaccagccaagcaacctcagacttccgcttgatgaaggcagtcgctgaagaaactcggctcagtcctatgggaaggcagcagcaactagcccgactggcagatgacatccagagaaaccaagtgactcggtttgagcttgagacctggggattgcattttggaagccagctatccctgaccggccgggttgttccctctgaaaagatcctgctgcaggaccacacatgtcaacctgcatttgctgctgattggtccaaagatatgcgatcttgcaaggttctgagttctgagcctttgaatacatggttgatcgtgtgctgtagcagggctgagcatttgattgaatccttcctgagctgtctgaggagagttggaggttccacgggattcagtgtgggccacccaaaaatcataaaagtggatgaaaaccctgcagcgttccttcgagccatccagctgcacgtcaaccccgatgttcagttggtgatgtgcatcctgccttctaatcagaagaactattacgactccattaaaagatatttgagctccgactgcccggtcccaagccagtgtgtgctgacccggaccttgaacaaacagggaatgatgctgagtgtggccaccaagatcgccatgcaaatgacctgcaaactcgggggcgagctgtgggctgtggagatcccattgaagtcactgatggtcgtgggcatcgacatctgtagagacgccctcagcaaggatgtagcggtcgtcgggtttgtcgccagcattaactccaggatcaccaggtggttttcccgctgtgtcctccagagaacggcagctgatattgcagattgcctgaaagtctgcatgactggtgctctcaacaagtggtacaaacacaaccacgatttgccggcacggataatcgcgtaccgggatggtgtgggcaacggccagctaaaggcagttttggaatacgaagtcccacagctgctgagcagtgtgacagaatgtggctccgacgccaggagctgtagactgtcagtggtcgtggtcaggaagaggtgtctgctgcgtctctttgccgagtctggccatacgctacagaaccccccacttggcactgtcgtggactcagaagcaacacgtccagagtggtatgacttctacctgaccagccagactgctaaccgggggactgttagtcccacctactacaatgtcatctatgacgacaacgctctgaagcctgaccatatgcagcggctgaccttcaaactgtgccacctctactacaactggcagggcgtaatcagtgtccctgctccgtgccagtatgcacacaagctgaccttcctggtggcacaaagcatccacaaggaaccaagtctggaattagccaataagctcttctacctgtgacaagtgggccaatagcatggctcgatggagacgccaaaaagcggttgctatgctttgttcaaatctgcctaatgctcaaggctcctgctgcttaaaggagcttcagcctgattttaagtattgggaaggagagggttttttggtttttgtttttttgttttgttttgttttgttttttgttttctgggttttttttttgttgttgttgttgttgtttttaaagtcatctgaatcagttgaggacaaacctatggggatttttgtctcccctgtgtctaaaaacaacaatgacaaacaaacaaaaacccacagagtgatgggttttcttgtggctctgtcaggcgacattttactttgtcctggtttatcctcgtatttttttttttttaagtaaatgcttgaggtgaattctcacatggtatatacgtgatgatattgggatatatcatcacagctaactggattctaaaacaatattcgtgtggtattgtgatggtgtttaagaggggtttaacttctttcctgacattaccagggttgttctaagtatcgtagagcattttgtagagtgggttaagtaaatactgaaaataaagactccctgagcatcg

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