LOCUS       NM_001012219            1907 bp    mRNA    linear   ROD 04-FEB-2025
DEFINITION  Rattus norvegicus LIM homeobox 8 (Lhx8), transcript variant 2,
            mRNA.
ACCESSION   NM_001012219 XM_345303
VERSION     NM_001012219.3
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Crews,F.T., Fisher,R.P., Qin,L. and Vetreno,R.P.
  TITLE     HMGB1 neuroimmune signaling and REST-G9a gene repression contribute
            to ethanol-induced reversible suppression of the cholinergic neuron
            phenotype
  JOURNAL   Mol Psychiatry 28 (12), 5159-5172 (2023)
   PUBMED   37402853
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Li,H., Qin,J., Jin,G., Zou,L., Shi,J., Han,X., Cheng,X. and
            Zhang,X.
  TITLE     Overexpression of Lhx8 inhibits cell proliferation and induces cell
            cycle arrest in PC12 cell line
  JOURNAL   In Vitro Cell Dev Biol Anim 51 (4), 329-335 (2015)
   PUBMED   25475040
  REMARK    GeneRIF: Lhx8 plays role as a suppressor gene of cell
            proliferation.
REFERENCE   3  (bases 1 to 1907)
  AUTHORS   Tomioka,T., Shimazaki,T., Yamauchi,T., Oki,T., Ohgoh,M. and
            Okano,H.
  TITLE     LIM homeobox 8 (Lhx8) is a key regulator of the cholinergic
            neuronal function via a tropomyosin receptor kinase A
            (TrkA)-mediated positive feedback loop
  JOURNAL   J Biol Chem 289 (2), 1000-1010 (2014)
   PUBMED   24265310
  REMARK    GeneRIF: Data indicate that LIM homeobox 8 (Lhx8) regulates
            functions of cholinergic neurons by modulating tropomyosin receptor
            kinase A (TrkA) expression.
REFERENCE   4  (bases 1 to 1907)
  AUTHORS   Zhu,P., Li,H., Jin,G., Tian,M., Tan,X., Shi,J., Zou,L. and Qin,J.
  TITLE     LIM-homeobox gene Lhx8 promote the differentiation of hippocampal
            newborn neurons into cholinergic neurons in vitro
  JOURNAL   In Vitro Cell Dev Biol Anim 49 (2), 103-107 (2013)
   PUBMED   23385486
  REMARK    GeneRIF: Although the potential interaction of Lhx8 with other
            factors remains unknown, our results suggested that neuronal
            subtype specification can be directly manipulated in the stage of
            immature newborn neurons by a specific transcription factor in
            vitro.
REFERENCE   5  (bases 1 to 1907)
  AUTHORS   Shi,J., Li,H., Jin,G., Zhu,P., Tian,M., Qin,J., Tan,X., Zhao,S.,
            Wang,F., Hua,Y. and Xiao,Y.
  TITLE     Lhx8 promote differentiation of hippocampal neural stem/progenitor
            cells into cholinergic neurons in vitro
  JOURNAL   In Vitro Cell Dev Biol Anim 48 (10), 603-609 (2012)
   PUBMED   23150137
  REMARK    GeneRIF: Lhx8 was not associated with the MAP2-positive neurons
            differentiation on adherent conditions, but played a role in the
            specification of cholinergic neurons derived from hippocampal
            neural stem/progenitor cells in vitro
REFERENCE   6  (bases 1 to 1907)
  AUTHORS   Pangas,S.A., Choi,Y., Ballow,D.J., Zhao,Y., Westphal,H.,
            Matzuk,M.M. and Rajkovic,A.
  TITLE     Oogenesis requires germ cell-specific transcriptional regulators
            Sohlh1 and Lhx8
  JOURNAL   Proc Natl Acad Sci U S A 103 (21), 8090-8095 (2006)
   PUBMED   16690745
REFERENCE   7  (bases 1 to 1907)
  AUTHORS   Fragkouli,A., Hearn,C., Errington,M., Cooke,S., Grigoriou,M.,
            Bliss,T., Stylianopoulou,F. and Pachnis,V.
  TITLE     Loss of forebrain cholinergic neurons and impairment in spatial
            learning and memory in LHX7-deficient mice
  JOURNAL   Eur J Neurosci 21 (11), 2923-2938 (2005)
   PUBMED   15978004
REFERENCE   8  (bases 1 to 1907)
  AUTHORS   Mori,T., Yuxing,Z., Takaki,H., Takeuchi,M., Iseki,K., Hagino,S.,
            Kitanaka,J., Takemura,M., Misawa,H., Ikawa,M., Okabe,M. and
            Wanaka,A.
  TITLE     The LIM homeobox gene, L3/Lhx8, is necessary for proper development
            of basal forebrain cholinergic neurons
  JOURNAL   Eur J Neurosci 19 (12), 3129-3141 (2004)
   PUBMED   15217369
REFERENCE   9  (bases 1 to 1907)
  AUTHORS   Shibaguchi,T., Kato,J., Abe,M., Tamamura,Y., Tabata,M.J., Liu,J.G.,
            Iwamoto,M., Wakisaka,S., Wanaka,A. and Kurisu,K.
  TITLE     Expression and role of Lhx8 in murine tooth development
  JOURNAL   Arch Histol Cytol 66 (1), 95-108 (2003)
   PUBMED   12703558
REFERENCE   10 (bases 1 to 1907)
  AUTHORS   Asbreuk,C.H., van Schaick,H.S., Cox,J.J., Kromkamp,M., Smidt,M.P.
            and Burbach,J.P.
  TITLE     The homeobox genes Lhx7 and Gbx1 are expressed in the basal
            forebrain cholinergic system
  JOURNAL   Neuroscience 109 (2), 287-298 (2002)
   PUBMED   11801365
  REMARK    GeneRIF: Combined expression of Lhx7 and Gbx1 plays a role in the
            development of the cholinergic system of the basal forebrain.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000002.1.
            
            On Dec 21, 2022 this sequence version replaced NM_001012219.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC090011.1, SRR21887620.1010993.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760476, SAMN16093042
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-294               JAXUCZ010000002.1  245927185-245927478 c
            295-456             JAXUCZ010000002.1  245926826-245926987 c
            457-578             JAXUCZ010000002.1  245923291-245923412 c
            579-799             JAXUCZ010000002.1  245921024-245921244 c
            800-903             JAXUCZ010000002.1  245920441-245920544 c
            904-999             JAXUCZ010000002.1  245915307-245915402 c
            1000-1183           JAXUCZ010000002.1  245911125-245911308 c
            1184-1907           JAXUCZ010000002.1  245904712-245905435 c
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q45"
     gene            1..1907
                     /gene="Lhx8"
                     /gene_synonym="Lhx7"
                     /note="LIM homeobox 8"
                     /db_xref="GeneID:365963"
                     /db_xref="RGD:1308749"
     exon            1..294
                     /gene="Lhx8"
                     /gene_synonym="Lhx7"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    169..171
                     /gene="Lhx8"
                     /gene_synonym="Lhx7"
                     /note="upstream in-frame stop codon"
     CDS             220..1230
                     /gene="Lhx8"
                     /gene_synonym="Lhx7"
                     /note="isoform 2 is encoded by transcript variant 2;
                     LIM/homeobox protein Lhx8; LIM homeobox protein 8"
                     /codon_start=1
                     /product="LIM/homeobox protein Lhx8 isoform 2"
                     /protein_id="NP_001012219.1"
                     /db_xref="GeneID:365963"
                     /db_xref="RGD:1308749"
                     /translation="

MSEECGRPAALAAGRTRKGAGEEGLVNPEGAGDEDSCSSSAPLSPSSSPQSMASGSVCPPGKCVCSSCGLEIVDKYLLKVNDLCWHVRCLSCSVCRTSLGRHTSCYIKDKDIFCKLDYFRRYGTRCSRCGRHIHSTDWVRRAKGNVYHLACFACFSCKRQLSTGEEFALVEEKVLCRVHFDCMLDNLKREVENGNGISVEGALLTEQDVNHPKPAKRARTSFTADQLQVMQAQFAQDNNPDAQTLQKLAERTGLSRRVIQVWFQNCRARHKKHVSPNHSSSAPVTAVPSSRLSPPMLEEMAYSAYVPQDGTMLTALHSYMDAHQQLLDSSPCYPIQ"

     misc_feature    409..576
                     /gene="Lhx8"
                     /gene_synonym="Lhx7"
                     /note="The first LIM domain of Lhx7 and Lhx8; Region:
                     LIM1_Lhx7_Lhx8; cd09381"
                     /db_xref="CDD:188767"
     misc_feature    order(412..414,421..423,475..477,484..486,493..495,
                     502..504,559..561,568..570)
                     /gene="Lhx8"
                     /gene_synonym="Lhx7"
                     /note="Zn binding site [ion binding]; other site"
                     /db_xref="CDD:188767"
     misc_feature    595..759
                     /gene="Lhx8"
                     /gene_synonym="Lhx7"
                     /note="The second LIM domain of Lhx7 and Lhx8; Region:
                     LIM2_Lhx7_Lhx8; cd09383"
                     /db_xref="CDD:188769"
     misc_feature    order(595..597,604..606,661..663,670..672,679..681,
                     688..690,745..747,754..756)
                     /gene="Lhx8"
                     /gene_synonym="Lhx7"
                     /note="Zn binding site [ion binding]; other site"
                     /db_xref="CDD:188769"
     misc_feature    865..1035
                     /gene="Lhx8"
                     /gene_synonym="Lhx7"
                     /note="Region: Homeodomain; pfam00046"
                     /db_xref="CDD:459649"
     exon            295..456
                     /gene="Lhx8"
                     /gene_synonym="Lhx7"
                     /inference="alignment:Splign:2.1.0"
     exon            457..578
                     /gene="Lhx8"
                     /gene_synonym="Lhx7"
                     /inference="alignment:Splign:2.1.0"
     exon            579..799
                     /gene="Lhx8"
                     /gene_synonym="Lhx7"
                     /inference="alignment:Splign:2.1.0"
     exon            800..903
                     /gene="Lhx8"
                     /gene_synonym="Lhx7"
                     /inference="alignment:Splign:2.1.0"
     exon            904..999
                     /gene="Lhx8"
                     /gene_synonym="Lhx7"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..1183
                     /gene="Lhx8"
                     /gene_synonym="Lhx7"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1907
                     /gene="Lhx8"
                     /gene_synonym="Lhx7"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

agtcctcgagtcgcccgctggcggcggcggcccagggaggctgcgcgctggccggccagctgcgcccggcctcaggtgcccgcggttgcacgcgccgccctcgtgcgcgcccttcaagagcgccctagggccgggactgcggggcgccgcgtgctcgccccgggctcctgacccgcgtcttcctgccttcccgctctcgcggcgcagtgccccgggctcatgtcggaggagtgcgggcggcctgcagccctggcggccgggaggacccgcaaaggcgctggggaagaaggactggtgaatcccgagggagccggggacgaggactcctgctcctcctcggccccgctttccccgtcgtcctcgccccagtccatggcctcgggctccgtgtgcccaccaggcaagtgtgtgtgcagcagctgcggcctggagattgtggacaaatacctcctcaaggtgaatgacttatgctggcatgtccgctgtctctcctgcagtgtctgcagaacgtccctgggaaggcacacaagctgttacattaaggataaagacattttctgtaaactcgattacttcagacggtatgggacacgctgctcccgctgtggaaggcacatccattctactgactgggtccgcagggccaagggcaacgtgtatcacttggcttgctttgcctgcttttcttgcaagaggcaactgtccacgggagaggagtttgccttggtagaggagaaggtcctctgtagagtgcactttgactgcatgctggacaatctgaagagagaagtggagaacggtaatgggattagtgtggaaggagcccttctcacagaacaagacgtcaatcatccaaagccagccaaaagagctcggaccagcttcacagccgatcagctccaggttatgcaagcacagtttgctcaggacaacaacccagacgcacagaccctccagaaactggcagaaaggacaggcttaagccggcgagtcatacaggtgtggtttcagaactgcagggctcgtcataagaaacatgtcagtcccaaccactcctcctctgccccggtcacagcagtcccctcatccaggctgtctccacccatgttggaagaaatggcttattccgcctacgtgccccaggacgggacgatgctgacggcgctgcattcatacatggacgctcaccaacaactcctggactccagcccttgctaccccatacaatgacccaactgccaataagtcatacttaattccttctttcagggatagaaatgattgaggatataaacctgtcatttattatgtataaaataccattgaaaagatattaatgttaattttttatttaacactcaaaggcatttcgaacatcctttcgccgtccacgtatgtacctagagttggcctgcaagacacttttattaattcttcattttggtttttttttttttgggtaaaacttatgtttacaagaagaaaacaagtcaaaacaattttttttgtattgtctggaaatagttggctccagtgtgtgtctattaacttatttgtcatcaaaagagcactttgcctaaaaggcaggactgatagtgtgcaaaaacgtttacaatcctttgtgaaaccgtagtttatcattagtttgtacctgtaagttattgttataaatattatctgtattttttgtcatatacaactttataccttgaagcttgtatctgtgaatttgcaactgaaatttattttgccagtgttttctggacgagcttgagtgcacgtccgttgtagcatgccgcacacacccacggcggtgtctgcacttggggagttctagctgtaaataacactctagtttacgaagcctaccatcctaagttcattaaacatttcgcatccttgtgaaa

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