2024-11-23 15:59:24, GGRNA.v2 : RefSeq release 226 (Sep, 2024)
LOCUS XM_066303918 4694 bp mRNA linear PLN 10-JUL-2024 DEFINITION PREDICTED: Oryza sativa Japonica Group endoribonuclease Dicer homolog 2b (LOC136351659), transcript variant X1, mRNA. ACCESSION XM_066303918 VERSION XM_066303918.1 DBLINK BioProject: PRJNA1123306 KEYWORDS RefSeq. SOURCE Oryza sativa Japonica Group (Japanese rice) ORGANISM Oryza sativa Japonica Group Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa. COMMENT MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_089043) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI RefSeq Annotation Status :: Full annotation Annotation Name :: GCF_034140825.1-RS_2024_06 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 10.3 Annotation Method :: Best-placed RefSeq; Gnomon; cmsearch; tRNAscan-SE Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Date :: 06/21/2024 ##Genome-Annotation-Data-END## FEATURES Location/Qualifiers source 1..4694 /organism="Oryza sativa Japonica Group" /mol_type="mRNA" /db_xref="taxon:39947" /chromosome="9" gene 1..4694 /gene="LOC136351659" /note="endoribonuclease Dicer homolog 2b; Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 1 mRNA, 12 ESTs, 736 long SRA reads, 3 Proteins, and 100% coverage of the annotated genomic feature by RNAseq alignments, including 48 samples with support for all annotated introns" /db_xref="GeneID:136351659" misc_feature 1 /gene="LOC136351659" /experiment="COORDINATES: cap analysis [ECO:0007248]" /note="transcription start site" CDS 183..4316 /gene="LOC136351659" /codon_start=1 /product="endoribonuclease Dicer homolog 2b isoform X1" /protein_id="XP_066160015.1" /db_xref="GeneID:136351659" /translation="
MGGPLTAAGGRGDGGAKAVEPLRPPPPPDPKTMARWYQLEALERAVRGNTLAFLETGSGKTLIAVMLLRAYAHRVRRPDSRRFAVFLVPTVVLVGQQARVVEQHTDLVVKQFCGEMGVDFWDAATWRSQLEDGEVLVMTPQILLDNLRHSFFRLQDIALLIFDECHHARGNTPYACIFKEFYHPQLNSSASDPLPRIFGMSASLIYSKDLNQHNYSKQISEIENLMNSKVYTVDSESALSEYIPFASTKIVHFDDSNISSELHANILSCLNRLTKKHIEALDRKLHGSSLENAKQRISKLHRTFVYCLYNLGVWLAAKAAEVQSYEENSLSFWGETLDKNVEGFIRNYSEEVHRELSCFLKNGHIGEKFPADSQDGILTPKVHCLIRTLLQYRHMQDLRCIVFVQRVITSIVLEPLLSSIHQMSGWNVKHMAGSRPGLLSQSRKNHTEIVESFRKGKVHIIIATQILEEGLDVPSCNLVIRFDPSATVCSFIQSRGRARMENSDYLLLVGRGDVEAHTNAKKFLASGQIMREESLRLGSISCQPLENTLCEDTYYRVESTPAFDIDKASGTCTLHLPKSSPVQTVNVEGEGSILKETVCLKACQELHAIGALTDSLLPELDVPCDEEPDIVVENKIEQPSYFPEEFVDNWRSFSRLGIYYCYKISLEGCPKTASPTDILLALKCDLGSDFTSSSFKLPGGQDNASVTMKYVGIIHLNQEQVIIARRFQTTILSFLIGDDHLEVSNGIKYFHEMQVPIGVVYLLLPLVSGRIDWCSMKFSSSPIYEANNKHMTHCHSCRDIDLLQTKDGPFCRCILKNSIVCTPHNNIFYVISGFLDLDANSRLPQHDGTVVTYKDYFKTRHGLTLTFENQPLLAGSKHVKVRNFLHNYYYKKEKEPGDRYSVELPPELCRIIMSPVSANNLHIFSYVPSIMFRIQCMLLSVKLKVQLGPTVQQFDVPVLKILEALTTKKCQEEFSQESLETLGDSFLKYVTTRHLFSEYRLQHEGILTKMKKNLISNAALCQLACSSNLVGYIHAEEFNPRDWIIPCLDYDERGNKKISFLAPNGMYSQRKMSIKSKRIADSVEALIGAYLSTAGEKAAFLLMKSLGMNIEFHTEIPVERKISMKAEEFIDVRSLEGMLGYKFNDSLLLLEALTHGSYQTSGPTSCYQRLEFLGDAILDHLFTEYYYSKYPDCTPELLTDLRSASVNNNCYAHAAVKSGLNKHILHSSSELHRKMSYYLGQSFTGPSYGWEAGIGLPKVLGDVIESIAGAIYLDSKCDKEVVWRSMKRLLEPLATPETIEPDPVKGLQEFCDRRSFKITYEKNHVDGVSSVIARVKAGETTYSATKSGPCKLAKKLASKAVLKDLIAGHKDTEAAAV"
misc_feature 282..887 /gene="LOC136351659" /note="DEXH-box helicase domain of endoribonuclease Dicer; Region: DEXHc_dicer; cd18034" /db_xref="CDD:350792" misc_feature order(282..287,294..296,345..368,483..485,672..674, 786..788) /gene="LOC136351659" /note="ATP binding site [chemical binding]; other site" /db_xref="CDD:350792" misc_feature order(447..452,519..524,597..599,603..608,615..617, 690..698) /gene="LOC136351659" /note="nucleic acid binding site [nucleotide binding]; other site" /db_xref="CDD:350792" misc_feature 1311..1709 /gene="LOC136351659" /note="Members of the P-loop NTPase domain superfamily are characterized by a conserved nucleotide phosphate-binding motif, also referred to as the Walker A motif (GxxxxGK[S/T], where x is any residue), and the Walker B motif (hhhh[D/E], where h is a...; Region: P-loop containing Nucleoside Triphosphate Hydrolases; cl38936" /db_xref="CDD:476819" misc_feature 1671..1673 /gene="LOC136351659" /note="ATP binding site [chemical binding]; other site" /db_xref="CDD:350189" misc_feature 1824..2036 /gene="LOC136351659" /note="Dicer dimerization domain; Region: Dicer_dimer; pfam03368" /db_xref="CDD:460900" misc_feature 2538..2927 /gene="LOC136351659" /note="PAZ domain, CAF_like subfamily. CAF (for carpel factory) is a plant homolog of Dicer. CAF has been implicated in flower morphogenesis and in early Arabidopsis development and might function through posttranscriptional regulation of specific mRNA...; Region: PAZ_CAF_like; cd02844" /db_xref="CDD:239210" misc_feature order(2667..2669,2739..2741,2751..2753,2805..2807, 2886..2888,2892..2894) /gene="LOC136351659" /note="nucleic acid-binding interface [nucleotide binding]; other site" /db_xref="CDD:239210" misc_feature 3063..3527 /gene="LOC136351659" /note="Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in post-transcriptional...; Region: RIBOc; cd00593" /db_xref="CDD:238333" misc_feature order(3111..3116,3123..3128,3135..3137,3144..3149, 3156..3161,3165..3173,3201..3203,3453..3455,3480..3482) /gene="LOC136351659" /note="dimerization interface [polypeptide binding]; other site" /db_xref="CDD:238333" misc_feature order(3111..3113,3120..3122,3132..3134,3423..3425, 3432..3434) /gene="LOC136351659" /note="active site" /db_xref="CDD:238333" misc_feature order(3120..3122,3423..3425,3432..3434) /gene="LOC136351659" /note="metal binding site [ion binding]; metal-binding site" /db_xref="CDD:238333" misc_feature 3624..4076 /gene="LOC136351659" /note="Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in post-transcriptional...; Region: RIBOc; cd00593" /db_xref="CDD:238333" misc_feature order(3684..3689,3696..3701,3708..3710,3717..3722, 3729..3734,3738..3746,3774..3776,3996..3998,4029..4031) /gene="LOC136351659" /note="dimerization interface [polypeptide binding]; other site" /db_xref="CDD:238333" misc_feature order(3684..3686,3693..3695,3705..3707,3966..3968, 3975..3977) /gene="LOC136351659" /note="active site" /db_xref="CDD:238333" misc_feature order(3693..3695,3966..3968,3975..3977) /gene="LOC136351659" /note="metal binding site [ion binding]; metal-binding site" /db_xref="CDD:238333" misc_feature 4089..4286 /gene="LOC136351659" /note="double-stranded RNA binding motif (DSRM) superfamily; Region: DSRM_SF; cl00054" /db_xref="CDD:444671" misc_feature order(4104..4109,4113..4115,4173..4175,4230..4235, 4242..4244) /gene="LOC136351659" /note="RNA binding site [nucleotide binding]; other site" /db_xref="CDD:380679" polyA_site 4694 /gene="LOC136351659" /experiment="COORDINATES: polyA evidence [ECO:0006239]" ORIGIN
gagcccaacacgcgcgcgcgcgactcgtaggaattcggttcggattcgtgcgtgcaccgaaggaggcggaggcggaggcggcccaagcgtcggcgccacctcactctcccacccccatcccgccgcctcccccgattcctcccccaaacccctcctcctcctccgccgccgctgccgtcgccatgggtggccccctcaccgccgccggaggtcgaggagatgggggcgccaaggcggtggaaccgctccggcctccgccgccgcccgacccgaagacaatggccagatggtaccagctggaggcgctggagcgggcggtgcgggggaacaccctggcgttcctggagaccgggagcgggaagacgctcatcgcggtgatgctgctccgggcgtacgcgcaccgcgtgcggaggccggactcgcgccgcttcgccgtcttcctcgtccccaccgtcgtcctcgtgggccagcaggcgcgcgtcgtcgagcagcacaccgacctcgtcgtcaagcagttctgcggcgagatgggggtcgacttctgggacgccgccacctggcgatcccaactggaggacggcgaggtgctcgtcatgacgccgcagatcctgctcgacaacctccgccacagcttcttccgcctccaggacatcgcgctgctcatcttcgacgagtgtcaccacgccaggggcaatacaccgtacgcctgcattttcaaggaattttatcacccccagttgaattctagtgcgtcagaccctctgccaaggatatttgggatgtctgcttcgctcatttattcaaaagatttgaatcagcacaattattcaaagcaaatttctgagattgagaatctcatgaattccaaggtgtacactgttgatagtgaatcagctttgtcggagtacataccatttgcttctacaaaaatagtgcactttgacgattccaacatttcatctgagctgcatgccaatattctcagttgtttgaatagattgacaaaaaagcatatagaagctctggacagaaaactacatggttcatctctggaaaacgctaagcaaaggatatcaaaattgcatcgtacatttgtatattgtttatataatctaggagtatggctggcagcaaaggctgctgaggtacaatcatacgaggaaaacagcctatccttctggggtgaaacattggataaaaatgttgagggcttcattagaaactacagcgaagaagtacatagagaactttcatgtttcttgaagaatggtcatattggtgaaaaatttccagctgattcgcaagatgggatattgacacccaaagttcattgccttattcgaactcttctgcagtacaggcatatgcaagacttaagatgcattgtgtttgttcagagagttatcacaagcatagtattagagcccctactttcatctattcatcaaatgtctgggtggaatgttaagcacatggcaggaagccgtcctggtttgctatctcagagcaggaaaaatcatacagaaattgttgaatcttttcgaaaaggaaaggtacatataattattgccacacaaattttggaggagggtttagatgtgccaagttgtaacttggtaattcgctttgatccatcagcaacagtatgcagctttatacagtcccgtggtcgagctaggatggaaaactctgactatcttttacttgtcggaaggggggatgtggaagctcatactaatgcaaagaagtttctcgcaagtgggcaaataatgcgggaggaatctctgagattgggatcaatttcttgtcaaccacttgagaacaccttgtgcgaggatacctattaccgtgtcgagtcaactccagcgttcgatatagacaaggcatctggcacatgcactctgcatcttcctaagagcagtcctgtgcagacagttaatgtagaaggagaaggttctattctcaaggagacagtttgtcttaaggcctgccaagaactacatgccattggtgcactcacagattctcttttaccagaattagatgttccatgtgatgaagaacctgacatcgttgttgagaacaaaattgagcagccttcttactttccagaagaatttgtggataattggcgctccttttctcgtcttggcatatattattgctataagatctcattggaaggatgtcccaaaacagcttctccaactgacatactgttagctttgaagtgtgatttgggatctgactttacttctagctcatttaaattgccaggtgggcaagacaatgctagtgttaccatgaaatatgttggcatcatccatctcaatcaagaacaggtaattattgctagaagatttcagacaactattctttcgtttcttattggtgatgaccatttggaggttagcaatggtattaaatacttccatgagatgcaagtgcctattggagtagtctatctacttctgcctttagtttctggcagaattgattggtgcagtatgaagttctcatcctcaccaatatatgaagctaacaacaaacatatgacacattgccattcttgtagagatattgatttactgcagacaaaagatggtcctttctgcaggtgcatccttaaaaattccattgtgtgtacccctcataataacatcttttatgttattagtgggtttcttgacttagatgcaaattcccgtttgcctcaacatgatgggactgttgttacctacaaagattattttaagactaggcatggtcttactttgacttttgaaaatcagccgctcttggctggcagtaaacatgtgaaagtgcggaacttcctccacaactactactataaaaaagaaaaagaacctggtgatagatatagtgttgagttgccacctgaactctgcagaattatcatgtcaccagtgtccgccaataatttgcacatcttctcatatgttccttccataatgttccgcatccagtgcatgcttctttctgtgaaattaaaggtccaattaggcccaacagtgcagcagtttgacgtaccggttttgaagatcctggaagcattaacaacaaagaaatgccaagaagaattttcacaggaatcattagaaacacttggggattctttcctcaaatatgttacaactcggcacctttttagtgaatacaggcttcaacatgaaggcatattaacaaaaatgaagaaaaacttgatctccaatgcagctctgtgccagctggcctgcagcagtaatcttgtgggttacattcatgctgaggagtttaatccaagagattggatcatcccttgcttggactatgatgagcgtggtaacaaaaaaatttcctttctggcacccaatggtatgtacagccaaaggaaaatgtctattaaaagtaagaggattgcggattcagttgaggccttaattggggcttacctcagtactgctggggaaaaggcagcatttcttttgatgaaatcattagggatgaatatagaatttcacactgaaattccagttgaaaggaaaatttcaatgaaagctgaagagttcattgatgtgaggagcttagagggaatgcttggttacaagttcaatgattctttattattgctcgaagcattgacacatggctcatatcagacttctggccctacttcatgctatcagaggctcgagtttcttggagatgctattttggatcatcttttcactgaatattactacagcaaataccctgactgtactccagaattgttgacagatcttagatctgcctctgtgaacaacaattgttacgcgcatgcagctgttaaatcaggattaaacaagcatatccttcactcatcatcagaactgcataggaagatgagctactaccttgggcagtcatttacaggtccatcttatggctgggaagcgggcattggtctaccaaaggttctcggggatgtgattgaatcaatagctggtgcaatttatctcgacagcaagtgtgacaaggaggttgtttggagaagcatgaagcggctattggaacctcttgccacccccgaaaccatcgagcctgacccggtgaaagggctgcaagaattttgtgatcgtagatcattcaaaataacctacgaaaaaaaccatgttgatggagtctcatcggtcattgctcgggtgaaggctggagagactacatactccgcaacaaaatcaggtccctgcaagttggcgaaaaagttggcatcaaaggctgtgcttaaagatctgattgctggtcacaaagatacagaggctgctgcagtatagtgatgcttaactatttaagcgtgcaacagtagctaacaattgatatagatagttgcgcacaatagaaaagttttggttggattcatacatgtaatttggaaatgctcctttttgtgtgctcgcaaacgatgcgtgtgaacggcctcatgaattctggtaggttttcatgcttcaaacttacaagtatcgcaattttgtaaattacaaatacgggcatagcacacctgcccatgccatgcagtagtttttttttcctcgaaatagtagcattagttgtatattactcatagcatcagattgtacagaaagcatgccagcaaaaacgaaaaaatgtacagacagcattttgttcgcatcaagggaaaacttgttttaaga
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If you use GGRNA in your work, please cite:
Naito Y, Bono H. (2012)
GGRNA: an ultrafast, transcript-oriented search engine for genes and transcripts.
Nucleic Acids Res., 40, W592-W596.
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