LOCUS       NM_001193659            3710 bp    mRNA    linear   ROD 17-JUL-2024
DEFINITION  Mus musculus claudin 12 (Cldn12), transcript variant 2, mRNA.
ACCESSION   NM_001193659
VERSION     NM_001193659.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Mak,S. and Hammes,A.
  TITLE     Canonical and Non-Canonical Localization of Tight Junction Proteins
            during Early Murine Cranial Development
  JOURNAL   Int J Mol Sci 25 (3), 1426 (2024)
   PUBMED   38338705
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3710)
  AUTHORS   Chen,J.T., Hu,X., Otto,I.U.C., Schurger,C., von Bieberstein,B.R.,
            Doppler,K., Krug,S.M., Hankir,M.K., Blasig,R., Sommer,C., Brack,A.,
            Blasig,I.E. and Rittner,H.L.
  TITLE     Myelin barrier breakdown, mechanical hypersensitivity, and
            painfulness in polyneuropathy with claudin-12 deficiency
  JOURNAL   Neurobiol Dis 185, 106246 (2023)
   PUBMED   37527762
  REMARK    GeneRIF: Myelin barrier breakdown, mechanical hypersensitivity, and
            painfulness in polyneuropathy with claudin-12 deficiency.
REFERENCE   4  (bases 1 to 3710)
  AUTHORS   Beggs,M.R., Young,K., Plain,A., O'Neill,D.D., Raza,A.,
            Flockerzi,V., Dimke,H. and Alexander,R.T.
  TITLE     Maternal Epidermal Growth Factor Promotes Neonatal Claudin-2
            Dependent Increases in Small Intestinal Calcium Permeability
  JOURNAL   Function (Oxf) 4 (5), zqad033 (2023)
   PUBMED   37575484
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3710)
  AUTHORS   Xing,W., Pourteymoor,S., Chen,Y. and Mohan,S.
  TITLE     Targeted Deletion of the Claudin12 Gene in Mice Increases Articular
            Cartilage and Inhibits Chondrocyte Differentiation
  JOURNAL   Front Endocrinol (Lausanne) 13, 931318 (2022)
   PUBMED   35937800
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3710)
  AUTHORS   Hashizume,A., Ueno,T., Furuse,M., Tsukita,S., Nakanishi,Y. and
            Hieda,Y.
  TITLE     Expression patterns of claudin family of tight junction membrane
            proteins in developing mouse submandibular gland
  JOURNAL   Dev Dyn 231 (2), 425-431 (2004)
   PUBMED   15366020
REFERENCE   7  (bases 1 to 3710)
  AUTHORS   Takasato,M., Osafune,K., Matsumoto,Y., Kataoka,Y., Yoshida,N.,
            Meguro,H., Aburatani,H., Asashima,M. and Nishinakamura,R.
  TITLE     Identification of kidney mesenchymal genes by a combination of
            microarray analysis and Sall1-GFP knockin mice
  JOURNAL   Mech Dev 121 (6), 547-557 (2004)
   PUBMED   15172686
REFERENCE   8  (bases 1 to 3710)
  AUTHORS   Kitajiri,S.I., Furuse,M., Morita,K., Saishin-Kiuchi,Y., Kido,H.,
            Ito,J. and Tsukita,S.
  TITLE     Expression patterns of claudins, tight junction adhesion molecules,
            in the inner ear
  JOURNAL   Hear Res 187 (1-2), 25-34 (2004)
   PUBMED   14698084
  REMARK    GeneRIF: Claudin-12 is expressed at the variety of epithelial
            tissues in inner ear including Organ of Corti, stria vascularis,
            Reissner's membrane, spiral limbus, vestibular sensory epithelia,
            and dark cell area.
REFERENCE   9  (bases 1 to 3710)
  AUTHORS   Nitta,T., Hata,M., Gotoh,S., Seo,Y., Sasaki,H., Hashimoto,N.,
            Furuse,M. and Tsukita,S.
  TITLE     Size-selective loosening of the blood-brain barrier in
            claudin-5-deficient mice
  JOURNAL   J Cell Biol 161 (3), 653-660 (2003)
   PUBMED   12743111
REFERENCE   10 (bases 1 to 3710)
  AUTHORS   Niimi,T., Nagashima,K., Ward,J.M., Minoo,P., Zimonjic,D.B.,
            Popescu,N.C. and Kimura,S.
  TITLE     claudin-18, a novel downstream target gene for the T/EBP/NKX2.1
            homeodomain transcription factor, encodes lung- and
            stomach-specific isoforms through alternative splicing
  JOURNAL   Mol Cell Biol 21 (21), 7380-7390 (2001)
   PUBMED   11585919
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC068663.5.
            
            On Nov 22, 2023 this sequence version replaced NM_001193659.1.
            
            Summary: This gene encodes a member of the claudin family. Claudins
            are integral membrane proteins and components of tight junction
            strands. Tight junction strands serve as a physical barrier to
            prevent solutes and water from passing freely through the
            paracellular space between epithelial or endothelial cell sheets,
            and also play critical roles in maintaining cell polarity and
            signal transductions. This gene, along with several other family
            members, is expressed in the inner ear. The protein encoded by this
            gene and another family member, claudin 2, are critical for vitamin
            D-dependent Ca2+ absorption between enterocytes. Multiple
            alternatively spliced transcript variants encoding the same protein
            have been found. [provided by RefSeq, Oct 2011].
            
            Transcript Variant: This variant (2) differs in the 5' UTR, as
            compared to variant 1.
            
            Sequence Note:.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK039672.1, SRR13948564.2363253.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-222               AC068663.5         33556-33777
            223-312             AC068663.5         35154-35243
            313-364             AC068663.5         37283-37334
            365-3710            AC068663.5         39951-43296
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 2.98 cM"
     gene            1..3710
                     /gene="Cldn12"
                     /note="claudin 12"
                     /db_xref="GeneID:64945"
                     /db_xref="MGI:MGI:1929288"
     exon            1..222
                     /gene="Cldn12"
                     /inference="alignment:Splign:2.1.0"
     exon            223..312
                     /gene="Cldn12"
                     /inference="alignment:Splign:2.1.0"
     exon            313..364
                     /gene="Cldn12"
                     /inference="alignment:Splign:2.1.0"
     exon            365..3710
                     /gene="Cldn12"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    367..369
                     /gene="Cldn12"
                     /note="upstream in-frame stop codon"
     CDS             394..1128
                     /gene="Cldn12"
                     /codon_start=1
                     /product="claudin-12"
                     /protein_id="NP_001180588.1"
                     /db_xref="CCDS:CCDS19074.1"
                     /db_xref="GeneID:64945"
                     /db_xref="MGI:MGI:1929288"
                     /translation="

MGCRDVHAATVLSFLCGIASVAGLFAGTLLPNWRKLRLITFNRNEKNLTIYTGLWVKCARYDGSSDCLMYDRTWYLSVDQLDLRVLQFALPLSIVIAMGALLLCLIGMCNTAFNSSVPNIKLAKCLVNSAGCHLVAGLLFFLAGTVSLSPSIWAIFYNSHLNRKFEPVFTFDYAVFVTIASSGGLFMTALLLFVWYCACKSLSSPFWQPLYSHAPGMHTYSQPYSSRSRLSAIEIDIPVVSHST"

     misc_feature    424..486
                     /gene="Cldn12"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9ET43.2);
                     transmembrane region"
     misc_feature    655..717
                     /gene="Cldn12"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9ET43.2);
                     transmembrane region"
     misc_feature    799..861
                     /gene="Cldn12"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9ET43.2);
                     transmembrane region"
     misc_feature    916..978
                     /gene="Cldn12"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9ET43.2);
                     transmembrane region"
     misc_feature    1075..1077
                     /gene="Cldn12"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P56749; propagated from
                     UniProtKB/Swiss-Prot (Q9ET43.2); phosphorylation site"
     misc_feature    1084..1086
                     /gene="Cldn12"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q9ET43.2); phosphorylation site"
     polyA_site      1666
                     /gene="Cldn12"
                     /note="major polyA site"
     regulatory      3689..3694
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cldn12"
                     /note="hexamer: ATTAAA"
     polyA_site      3710
                     /gene="Cldn12"
                     /note="major polyA site"
ORIGIN      

agaacgcacttccgcggcagcggagggagaccaaggagccgcggctcggagtgggtgagcgggatgcccgagccgtgaggagccggtaggtgtggtcagggacgctcccgtttccgagtcttttgcaagtagttgaaggtgttgcgtgaaacggcgaagcggcggcgagtcggcatttgaaaggttgcccacatgggtggacgtggaaaagtggccgaggaggtccagattatctctgctttgtgtgcagatgtgctcctgttgcatctgagttcactaagctgactttggaggatcaggcacagggaaaggggatcagaggcgcagacaggctgcttggagaaacgctgattaccttgaaattactgaccatacagctcaagccctcctgccatgggctgccgagatgtccacgcagccaccgtcctgtccttcctgtgtggtattgcctctgtcgcaggcctctttgcggggactctgcttcctaactggaggaaactgcggctgatcacattcaacagaaacgagaagaacctgacgatttacacgggcctgtgggtgaagtgtgcccggtatgatggaagcagtgactgcctgatgtacgaccgtacgtggtacctgtcggttgaccagctggacctgcgtgtcctccagtttgccctgcctctcagcatcgtgatcgcaatgggtgccttgctactctgcctgattggaatgtgtaacacggccttcaattcttccgtgcctaacatcaaactggccaagtgtctggtcaatagtgcaggctgccacctggtggccggactcctgttttttctggcaggtaccgtgagcctctctccgtccatctgggccatcttttataacagccatctcaacaggaagtttgagccggtctttacctttgactatgcagtatttgtcactattgctagctcagggggtctgtttatgactgctctcctgctgttcgtttggtattgtgcatgcaagtctttgtcctctcctttctggcaaccgctgtactctcacgctcccgggatgcacacttactcacagccctattcatcacggtcccgcctctctgccattgaaatcgacattccagtagtctcacacagcacttaacagagatacaattaactgttcaaaggatgtcttgtagcctcacagccccctgcataatgagcctttttggacctgtgtacatttttcctatgttccttatcagtcagtggagccaaatttgtatatcttggtagaatgaaatgctgctagattttatgaggtacatattacattcttctaaatgtgaaccattccttttatcttgcttctgagactagaagggtctctagcctccttgttgatgtctgatcaatagcttatgtgaaaggacccatgataacactggaggcagtgaagacagcatattgaacaatgctgtttgatcttcacccgtatttcagattgtgcattgactccgatgattgaaaggtggaaggggcagtttcaaacttaagacgtttcagtcggatgaggctaggagtttgtctgtctccagaactcttccagcttgaaggtataatttcattctctctggggactaattgaataggcatcagtatactgaagattgctatcttctacctcattccagggaaaagtaaaacgcattttacatgacagagtaaagctaatatttaagcatggttaacatttggcttcttcattggtttcagatggggtcttgctgtgtagctctggctggcctagagatcactgcagaccagtgtgtactcagactttctacccgcttgcctcctgagcactaactacaaggcacacccaattacactaacaatgcgtcaggagacagtcagtatgttgcttcatttgaagcaagaatccctttccagatccccttatcaggtagtgctcatgcgctggccaagctcttagtgcctaggacaaaattgtttttcagggcgcgggagtctgttctttagtgtcagcgccaaagattcatttggtggctactatttgagtagtggctttagtctgttcagttcctgcttcagaactgatttcttgcttctgacactgcttaacgtttgagtaagtacttcagaggatacacaaataggagaaatagccatgtctatgtaggaatgttgatctaattcacctgataaccagtgggaagtctgacattatggtagctggtaagcttgtcaagcattgacccaggagtgtatttcatctgaaagatgtctttgtgacacagacagcctgtggtccaccaccagcaatagcctggacgctggaaattccctttaatcaggtctcctcccatagccgtaataatggtctcagatcatgagaatggtaagataggagagagattaaattatacctattctacctctgaagctaagatgataaattaaagaggggcaaattttaaaaatatctcttctgatgtgatatcaaatggataaaaccatgttagtactaacatgacattgaggggtgttgggtcttagcttagggatttgagctgctcatttagataatgtccacgtactagcattgtttgtaaggactgtagtacattgtgcttatagatttttacaagcagctgattttatgataacagtttctgatgcctcttttgcaaatctgagtctttctgtagagatatgtagtacttttccctaagtccaacactactgtatgtaggccaacactactgtatgtaggcaagttaatccaattgtataattttaaaacgccgtcaatacaaagcatgcacatctatggttttttaaaattacaacttctagccttttccacctctggttcttctcactcataaatggagctgagtgttggtcattccaagacagtggcagctcgaattctgagactaataatagtgttttccctttgttaattaatcttagttcttctctcactaaactgtttccagatggaattctaccatgccttggtgttagtatacagagataaaagtagggccaggtgtagtggcttttgactttaataccagcactcaggaggcagtgacaggtgcatttctggaaattctggtctccatagcaagttccaggccagtcagagctacacagtgagaacctgtctttaaaaacaattagaaaaagtggactcagcaatgagaatactagacagttttcagctcacaggattgtaactacaaacatttaagctttcttatgagaatctttcggcattatcatctatagcctcaaattactgttatgaattcactcaggaagagaaacagctgtttctgtattatctttttagtgttccagattgggcataaatagctgttttttaaagccatcaaaggaaagcacgtatttacaatgcttttgagtcactggggctgttattgagatggggtggataacttcttttgttgctaaaaagctgttgattttaaaggccttctaaggagccagttaggcaaagaaccttgaatgtgagacggtgcatagaatttctgttacccagctttgggagaagaataaacctgagattctgagttcactctgcttgtacatacgccctatgacggatagtgtgtgctgtaaaccacaaggtagaatcctcagtgggtgtgctgtgctgcctcatgtttattaaaagatgtatttttacaa

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