LOCUS       XM_054360300           14718 bp    mRNA    linear   PRI 26-AUG-2024
DEFINITION  PREDICTED: Homo sapiens argonaute RISC catalytic component 2
            (AGO2), transcript variant X4, mRNA.
ACCESSION   XM_054360300
VERSION     XM_054360300.1
DBLINK      BioProject: PRJNA807723
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_060932) annotated using gene prediction method: Gnomon,
            supported by mRNA and EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_009914755.1-RS_2024_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           RefSeqFE; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/23/2024
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..14718
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /isolate="CHM13"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /sex="female"
                     /cell_line="CHM13htert"
                     /tissue_type="hydatidiform mole"
                     /note="haploid cell line"
     gene            1..14718
                     /gene="AGO2"
                     /gene_synonym="CASC7; EIF2C2; LESKRES; LINC00980; PPD;
                     Q10"
                     /note="argonaute RISC catalytic component 2; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 10 mRNAs, 29 ESTs, 3800 long SRA reads, 13 Proteins,
                     and 99% coverage of the annotated genomic feature by
                     RNAseq alignments, including 5 samples with support for
                     all annotated introns"
                     /db_xref="GeneID:27161"
                     /db_xref="HGNC:HGNC:3263"
                     /db_xref="MIM:606229"
     CDS             384..2825
                     /gene="AGO2"
                     /gene_synonym="CASC7; EIF2C2; LESKRES; LINC00980; PPD;
                     Q10"
                     /codon_start=1
                     /product="protein argonaute-2 isoform X3"
                     /protein_id="XP_054216275.1"
                     /db_xref="GeneID:27161"
                     /db_xref="HGNC:HGNC:3263"
                     /db_xref="MIM:606229"
                     /translation="

MDIPKIDIYHYELDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA"

     misc_feature    519..743
                     /gene="AGO2"
                     /gene_synonym="CASC7; EIF2C2; LESKRES; LINC00980; PPD;
                     Q10"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    771..923
                     /gene="AGO2"
                     /gene_synonym="CASC7; EIF2C2; LESKRES; LINC00980; PPD;
                     Q10"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    924..1286
                     /gene="AGO2"
                     /gene_synonym="CASC7; EIF2C2; LESKRES; LINC00980; PPD;
                     Q10"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1080..1082,1125..1127,1167..1169,1179..1181,
                     1233..1235,1254..1256,1260..1262)
                     /gene="AGO2"
                     /gene_synonym="CASC7; EIF2C2; LESKRES; LINC00980; PPD;
                     Q10"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1419..2696
                     /gene="AGO2"
                     /gene_synonym="CASC7; EIF2C2; LESKRES; LINC00980; PPD;
                     Q10"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1830..1832,1842..1844,1878..1889,1896..1898,
                     1920..1922,1929..1931,1941..1943,1953..1955)
                     /gene="AGO2"
                     /gene_synonym="CASC7; EIF2C2; LESKRES; LINC00980; PPD;
                     Q10"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2034..2036,2040..2042,2250..2252,2664..2666)
                     /gene="AGO2"
                     /gene_synonym="CASC7; EIF2C2; LESKRES; LINC00980; PPD;
                     Q10"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

ctgtttggcgaggcggccgcgccggggcggatcgtgcggccggcggctccctcgcggctcgcggcgtcggggcccgtggcgcgcgcgcggccgcccctcggccccggagcccctcggcggcgccaccatgtactcgggagccggccccggtactaactgacttgtccatttgctaagcttcatcccaccgggcaattttcctgcacagcagctgttacagtcccattctcacctggcccctgggccagttcggaagcctctgagcagcacttgcacctcctgcgccgccgccccccatccaaggatatgccttcaagcctccacctagacccgactttgggacctccgggagaacaatcaaattacaggccaatttcttcgaaatggacatccccaaaattgacatctatcattatgaattggatatcaagccagagaagtgcccgaggagagttaacagggaaatcgtggaacacatggtccagcactttaaaacacagatctttggggatcggaagcccgtgtttgacggcaggaagaatctatacacagccatgccccttccgattgggagggacaaggtggagctggaggtcacgctgccaggagaaggcaaggatcgcatcttcaaggtgtccatcaagtgggtgtcctgcgtgagcttgcaggcgttacacgatgcactttcagggcggctgcccagcgtcccttttgagacgatccaggccctggacgtggtcatgaggcacttgccatccatgaggtacacccccgtgggccgctccttcttcaccgcgtccgaaggctgctctaaccctcttggcgggggccgagaagtgtggtttggcttccatcagtccgtccggccttctctctggaaaatgatgctgaatattgatgtgtcagcaacagcgttttacaaggcacagccagtaatcgagtttgtttgtgaagttttggattttaaaagtattgaagaacaacaaaaacctctgacagattcccaaagggtaaagtttaccaaagaaattaaaggtctaaaggtggagataacgcactgtgggcagatgaagaggaagtaccgtgtctgcaatgtgacccggcggcccgccagtcaccaaacattcccgctgcagcaggagagcgggcagacggtggagtgcacggtggcccagtatttcaaggacaggcacaagttggttctgcgctacccccacctcccatgtttacaagtcggacaggagcagaaacacacctaccttcccctggaggtctgtaacattgtggcaggacaaagatgtattaaaaaattaacggacaatcagacctcaaccatgatcagagcaactgctaggtcggcgcccgatcggcaagaagagattagcaaattgatgcgaagtgcaagtttcaacacagatccatacgtccgtgaatttggaatcatggtcaaagatgagatgacagacgtgactgggcgggtgctgcagccgccctccatcctctacgggggcaggaataaagctattgcgacccctgtccagggcgtctgggacatgcggaacaagcagttccacacgggcatcgagatcaaggtgtgggccattgcgtgcttcgccccccagcgccagtgcacggaagtccatctgaagtccttcacagagcagctcagaaagatctcgagagacgctggcatgcccatccagggccagccgtgcttctgcaaatacgcgcagggggcggacagcgtggagcccatgttccggcacctgaagaacacgtatgcgggcctgcagctggtggtggtcatcctgcccggcaagacgcccgtgtacgccgaggtcaagcgcgtgggagacacggtgctggggatggccacgcagtgcgtgcagatgaagaacgtgcagaggaccacgccacagaccctgtccaacctctgcctgaagatcaacgtcaagctgggaggcgtgaacaacatcctgctgccccagggcaggccgccggtgttccagcagcccgtcatctttctgggagcagacgtcactcacccccccgccggggatgggaagaagccctccattgccgccgtggtgggcagcatggacgcccaccccaatcgctactgcgccaccgtgcgcgtgcagcagcaccggcaggagatcatacaagacctggccgccatggtccgcgagctcctcatccagttctacaagtccacgcgcttcaagcccacccgcatcatcttctaccgcgacggtgtctctgaaggccagttccagcaggttctccaccacgagttgctggccatccgtgaggcctgtatcaagctagaaaaagactaccagcccgggatcaccttcatcgtggtgcagaagaggcaccacacccggctcttctgcactgacaagaacgagcgggttgggaaaagtggaaacattccagcaggcacgactgtggacacgaaaatcacccaccccaccgagttcgacttctacctgtgtagtcacgctggcatccaggggacaagcaggccttcgcactatcacgtcctctgggacgacaatcgtttctcctctgatgagctgcagatcctaacctaccagctgtgtcacacctacgtgcgctgcacacgctccgtgtccatcccagcgccagcatactacgctcacctggtggccttccgggccaggtaccacctggtggataaggaacatgacagtgctgaaggaagccatacctctgggcagagtaacgggcgagaccaccaagcactggccaaggcggtccaggttcaccaagacactctgcgcaccatgtactttgcttgacatgttttagtgtttagcgattgtgtaccgagtgggattcacgagaccagctacactcagaccaacagatggccagcccttccgtgacagccagcatcgaacatgagacgtcattgattttattagattctccgttttccagaatgccttccgtcccagatttcaaacttggattttgaactgcagacctgtatgagaacccaatgtcataggaaatatggtttgctaaaatctataagctgcttattaaaacagagtcccgtgtgtcctaaaaatctcctaaaaccagtctatgaactcagggctttaaaacatttttaatttatttggtcattcaatttacttgtttttaatacatgattctctatgaaattgatgggctcaaactagctgtgaatcttctgagagtgaaagcaacacaaaacacaagtgtggttttaaagccttgaacattctgatgttgtcactaaagttgatttccaggcgatgctcgtgtgcccctggcgtggctcacccaagttcctcgactgagggccggtggccatgcagaggcgtccgcaggtgccgtgttctgccagcaccgcccttcacccggcctgaactaaggagcagtggcagaaggtgggccccgtgtgtttacagcatttccaggtccagagaggttggcagacaagtgccattttaataaaaaatgtatttaaaaaaagaaaaaagacaatatactgacagtctaatcatcagatttgtcctataggactgtgacatttattttccaaagtttttaaagaatacgggtctgtggtgataaatacagtacaatcctttttcactgttatgtctttaatgtaagagaagaatatatatatctggtttgcagtattaatgtgatagatagatgctgttttttcattttattgactatggggtgcttctgtgatctgtttggcatatcaagagtgttttggagcctggagatgggaccgcctctcagttcttagtgcttaaggacaggaatatgcaattgacttaatgtgacagcaacccgcttcccccgtggagactgtgcagcgtgtacccatgggagggacggatgtgccctccgcgccgctcgccaccctccctgccgcctcatccccgtgtgtcactcactcagctgatgaagcacaagtggaaattatttattcttgcattgctgggaaacattgatgtgtggggactcactcctctccgtgtgctcttttgtcagatgacttccatagatctatgcactgagattcgtagaaagctgttggtggtggctctgctgggcgtgaggccgtcggtcacagcgaaggagcttgcctggccctgggaggcccaaggatagcagggccagcagagggcatgggccgttcttctggggtttgcatttggggccccagacttgggtttgccaatctagaaactgttgcactaagagtgacaccacggacgattttgaaggctaacaacagacaactctgtttgtgctgagtgggctttttgtctctgtacacagttttagtaagactcaaatccatgttccgtgtagaggcgtcaggaaggaggcgagcctcccctagtgctgctgtgcgctccgtaggtcccctcttcctgctgctgttccctccccggccctgcgcgacacttacctcggcactgtggtggggtggggtgccaaaggagctgcctcctgctcagcggcaccccccccccacctttgtgctcagctgctttggaggcagcaggaggggcagcaagccgcaggtcgggggtctcccgatacctcgcctggatattggcggcaatgacaaagggatgactattatttgtgaagatgtgtactcagagtggtgtgtgtgtgtgtgcatgcgtgtgtccctgtgtgtgagtgtcttattaatggcattagtctcttctggctgtatttttttttaatctttttaaatgacagaagctcccactctaagaaatggcagttccttttgttttagtggcaagtggcactttctcctgttttcagaaggatacagacatgctcgtagcttaaattaatggatgcattcaagctaaggagggaatcttgtgactaataacttttcaaatctgaaattagattagtttccagccatttttacaatcatctggaaccatgtgtctcaaaggtgtgtttcttccacctgcgtcaggctgtctgggggatggcggttcttgtttaacatgcagatttgtgggcctgctgccacctcctgcgtgcggtacttcagggatggccagggcatctgtgtctaagcgatggcctgcagccctgggctccctggggtctgaggacccagctctggtggcacccagcagggaggaggtgcttcctgagcagatgcctgagacagactgggctggcagaaaagtaggactacaatttttgttttttttaatcattttgaaggaaaactttcatttttagtgggccagagtacttcgaaaagggtacttttttatagccctatgcttgttttataaaacttttaatactaaaaacattaatagaccaaacatctccagctgggtcagctgcacacctttgtacatgtctgatgattcagagtttaaatggctgtagttacaatttctcaggtaaacaattgttttctcaaatttttaccaatccctgcattattaggataaattttgagacagtgtaaaaacctcaaagtacagtagggtaagtcccagccctgttcacgtgaaagccagaggtggcccttccccggcatctgatcgtctgatcatgtgggtgcctggggccttgccacccttccctgtccactgctgcccccctgggcctgtcaagatttgaggggtgcccactcgctggctttgtccccatcccactgttaagtaaggtgtgccttgctttaagcaaactgaatgtgaaaattatgtgtagaagtctgggtttgtttaaaagaaaaactgggattttatctcatctctttaaaagaggtctcccaattcgatttctgtactgggactcttcctggtgtgtttttacatgttcactttttaaaaacattgatgtactcccatccttttaccactaccctatcacatttgacaagttcttgctgtaatgacgacgcaattaacgaacaccaggggagcggtggaaagctgccctcgtgtgtgagcccttcggtgacgcaaagccaccgtcctgttgttttgtgtgtcctaagctcagattttcctggtttggataaattttgtaaaactgggcatagctgtacttttagaaagggatcgttttccttaagttttggttttctctggttcaagcagtctttacatgcagccttttgattgcacatcagggtctcccttttcttttccagatgatgttgcttttcggtggtggtctcacactcctttgaatgaatgtttgcactctagaaccacaaacgatgcgtgaccattgtacagcttgcccaagagggaactgttggaagcaggcagtgacctccacgctgtttgtttgaggctgacctaaactgctatcactaatgtttacccccgtgacggagtgcccattagaacagatcatgtcagttcagttcctgtagtggggttagtaggtggggggttttattgttgttgttatttgttacttttattgctgttaaatgcaaatattgctatttattttttcctttctgctctggtttggtttggccgacacagatattccagtgcatgtggtacctaatggatatgatagaaattatttccccaacttttataaattgcctaatgtttaccttcaagtttgctggatgatttatttttgaaagccacaaatgtgatttttctggatgaattgtttttcttatatatgcagcaatgtaattttacattgatctgcggggtggtttaaattttatttttacaaggcagtttttcagtcaaattatatatttgatacaatacgctaggatagaccggccacacgtcaggcagccgggacaccggagtgtccccaggaggcctttggggctggggtggcccgtggcttgttccctgcgcctctgggaactggatgagtccttcggcgggactgtgacccgctggacagttaaaaggatttttcatgggcattttcctacacataaggcagttccattaggaggagctttagaagtcgacataatccagacagtggacaatcccagtggcccagacaagccatacgacattttcctgtgacctctctctgacccatctctcacgctcatgagaaaaggaatgtacaataaatagaaacagaaacttcctcagtgctcatgcgcctcacttgcagaacgtgagtatttcatttatgtttgaagcccttccccaaagtgaccgaggagttgcactctagctgccgtgccagcagtctcgtctgctgtgacgcttctcggtgcggatgcagtgcacctgtgactttgtcatgggggtgcccctgttacacacacacacgctgactcttttttatgaggaaaatcttcgactcaaaataattgcactgattttttgacaacttctttttacgatgtcccacttcgcatctgctgtacttgtttgagatgattgtaaatagatttgtggtaggagaagtgacatgaatgcaacacgtggctctgttgtggagacactgcctcgtattcagtattatgtgttaaagttgtgtggacttaactctgttccgccctttagcttcgtgtctgttgaccatttatgaaggacaagttcgcctcattttctttttcttctgaagggaagttctagatcggttactaggagaacatggtctcttggtgcctgatgatttagtcctaagcagactcgttgcagtcttgtgaacactgttgtggtctgtttgattcctcgtcgcttaccgtggctctccaaagcatggtctttgagttccacccagcctgggacagtccagacgggaggcatctgctccgcctgctactaggacgcaagggcctccttgcatctctgtggtcagagtcatcttaaagccatgaggttactgtcgtgtcaaagctgtcatttgtgcccgttattttgctggagtagcagcgtgtgctgtagcccccgtgtgtcactctgtggtcaggctaccctgtgtcacacccagcaagaagtttttcagccccagaccagtctgtgtgtgtgtccgagtcacaccagtgccattacggtgccctcgcttatgcttctttcagtatgttttgccatgttattgtctaaagtgaatgtattggcttagccaaatcactacagaaggtggttcctaaataccttttagtttaaaaaaaaatgcagataaaggctacctctgaattctcaatagattcatcatgtttgctcttaagtgtagctgtccacactgaaaacagccaatatgttgcctgaataaactgaattgtgaacatctatctgttcagttctggcttgaaaatgtgtgtgccatactgtgacccacgggcagcccctcctcctctactgtgtcaggtggaccagggtcacctctgttctgcgcagctttgagattctaggattctacggccggcacgaatggcatgggagggttctctgcacgggacggcataacggcatgccatccttcaggctggcaggagcctgcgcaggtgtggcaaaatcttgaaacagcctgtgtcctgcctggcttttcactttcctatttaatataagaaagcacttttttttctgctttacctacaaatgggttgaaaatggcctcctctgtcctctcctctcttttatacactctgtaaaatcacaaaggtgcttcaacaccgactgtcatgcaaataatacatttaaaatagttgcatggtttcaaagacaatgcagacgtaattggcctttgactttgggacctcgggttactctcactccctactgcaaaacaaactaagggttgaaagcaaagcattctgttcaaatccagggccctttgtttgaaaagaacacagggcccacagctggtgttttgaagtagaagttcccactagtggcgcagtggaccgtctgatgctgacttgcaggaagtcggtcctgggaggctctgaacacccctccctgtgattaaccactttgctgtcagaaatgtcattttcccatacactgtgctgtgggcccggggcctccttcgcttctttctttgggttccatatgttgcccgatcttgaggttagcgctcgccccaatttctccatgagtttactctgagttgacaggcgatgatctgacagttggaggtgtctgggtgtttggaggtggccccgttctcatgttggctgctcatcagacattgagtttggccccggcggttctactacgtcactacctggaagcaattgcctttaacactctgctgtttggggtttcgatgctttttatgctgcgttgcgtgctttcattccggaagagctaagtgtcctttgcagtctctctgagaatgttttgtaattctgccgccacgctgttctgtgcggaccgacctcaagaggcttgggcccccgtgggtgtcccactgcctgccggcccagggtacaggctgcttgggtccgctgggctggccttgggctgtcagaatctggtccagggtgtttcgaggggacactgcacagccgctcgcccctcgactcagtcatgggagctgcagcacgcagatccaccgcaagcttcctgcacgcgcatctccggaatgctgcgctgggccctcccaccatcttcgcgtttgtgagaatttactcagaagtgagagaactgaagattcctggtgcttttgttaacacgtttctatcttattggttggtgtggaaatgttagatgtgttgttaggtttttaaatgctgtgggtggtatgattaaagggttaagtcctcacctcagcccccagcccccttttggggggtaggaagaggctgagttatctcctatttaaaacctccttggatttaagtagttcagagttaaagctaaaacaccaaatattaactctttcccccaagtgtctccaggaactggacgttggggccattcttattcagttctttctgtagctccaaggtccttaaaatgag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