LOCUS       NM_001198710            2544 bp    mRNA    linear   VRT 10-DEC-2024
DEFINITION  Gallus gallus prolyl 4-hydroxylase subunit beta (P4HB), mRNA.
ACCESSION   NM_001198710 XM_420095
VERSION     NM_001198710.3
KEYWORDS    RefSeq.
SOURCE      Gallus gallus (chicken)
  ORGANISM  Gallus gallus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
            Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes;
            Phasianidae; Phasianinae; Gallus.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Tang,H., Finn,R.D. and Thomas,P.D.
  TITLE     TreeGrafter: phylogenetic tree-based annotation of proteins with
            Gene Ontology terms and other annotations
  JOURNAL   Bioinformatics 35 (3), 518-520 (2019)
   PUBMED   30032202
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Burge,S., Kelly,E., Lonsdale,D., Mutowo-Muellenet,P., McAnulla,C.,
            Mitchell,A., Sangrador-Vegas,A., Yong,S.Y., Mulder,N. and Hunter,S.
  TITLE     Manual GO annotation of predictive protein signatures: the InterPro
            approach to GO curation
  JOURNAL   Database (Oxford) 2012, bar068 (2012)
   PUBMED   22301074
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2544)
  AUTHORS   Krishna Rao,A.S. and Hausman,R.E.
  TITLE     cDNA for R-cognin: homology with a multifunctional protein
  JOURNAL   Proc Natl Acad Sci U S A 90 (7), 2950-2954 (1993)
   PUBMED   7681992
REFERENCE   4  (bases 1 to 2544)
  AUTHORS   Parkkonen,T., Kivirikko,K.I. and Pihlajaniemi,T.
  TITLE     Molecular cloning of a multifunctional chicken protein acting as
            the prolyl 4-hydroxylase beta-subunit, protein disulphide-isomerase
            and a cellular thyroid-hormone-binding protein. Comparison of
            cDNA-deduced amino acid sequences with those in other species
  JOURNAL   Biochem J 256 (3), 1005-1011 (1988)
   PUBMED   2851999
REFERENCE   5  (bases 1 to 2544)
  AUTHORS   Nakazawa,M., Aida,T., Everson,W.V., Gonda,M.A., Hughes,S.H. and
            Kao,W.W.
  TITLE     Structure of the gene encoding the beta-subunit of chicken prolyl
            4-hydroxylase
  JOURNAL   Gene 71 (2), 451-460 (1988)
   PUBMED   2852147
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAENSK010000317.1.
            
            On Sep 23, 2021 this sequence version replaced NM_001198710.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CR353567.1, HAEL01004007.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA103992692 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-195               JAENSK010000317.1  4668257-4668451     c
            196-402             JAENSK010000317.1  4667923-4668129     c
            403-536             JAENSK010000317.1  4667161-4667294     c
            537-674             JAENSK010000317.1  4666815-4666952     c
            675-779             JAENSK010000317.1  4664283-4664387     c
            780-905             JAENSK010000317.1  4663888-4664013     c
            906-1106            JAENSK010000317.1  4663351-4663551     c
            1107-1227           JAENSK010000317.1  4662697-4662817     c
            1228-1409           JAENSK010000317.1  4662303-4662484     c
            1410-1496           JAENSK010000317.1  4661766-4661852     c
            1497-2544           JAENSK010000317.1  4660121-4661168     c
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Gallus gallus"
                     /mol_type="mRNA"
                     /db_xref="taxon:9031"
                     /chromosome="18"
                     /map="18"
     gene            1..2544
                     /gene="P4HB"
                     /gene_synonym="PDI"
                     /note="prolyl 4-hydroxylase subunit beta"
                     /db_xref="CGNC:5464"
                     /db_xref="GeneID:374091"
     exon            1..195
                     /gene="P4HB"
                     /gene_synonym="PDI"
                     /inference="alignment:Splign:2.1.0"
     CDS             36..1583
                     /gene="P4HB"
                     /gene_synonym="PDI"
                     /EC_number="5.3.4.1"
                     /note="protein disulfide-isomerase; procollagen-proline,
                     2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta
                     polypeptide (protein disulfide isomerase; thyroid hormone
                     binding protein p55); prolyl 4-hydroxylase beta-subunit;
                     procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline
                     4-hydroxylase), beta polypeptide (protein disulfide
                     isomerase-associated 1); R-cognin; retina cognin; cellular
                     thyroid hormone-binding protein; prolyl 4-hydroxylase,
                     beta polypeptide"
                     /codon_start=1
                     /product="protein disulfide-isomerase precursor"
                     /protein_id="NP_001185639.2"
                     /db_xref="CGNC:5464"
                     /db_xref="GeneID:374091"
                     /translation="

MAVVRLRAIVALLCLVPPLGLAEPLEEEDGVLVLRAANFEQALAAHRHLLVEFYAPWCGHCKALAPEYAKAAAQLKAEGSEIRLAKVDATEEAELAQQFGVRGYPTIKFFRNGDKAAPREYTAGREADDIVSWLKKRTGPAATTLTDAAAAETLVDSSEVVVIGFFKDVTSDAAKEFLLAAESVDDIPFGISSSADVFSKYQLSQDGVVLFKKFDEGRNNFEGDLTKDNLLNFIKSNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYEGKLDNFKTAAGNFKGKILFIFIDSDHSDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESDDLTADKIKEFCNKFLEGKIKPHLMSQDLPEDWDKQPVKVLVGKNFEEVAFDENKNVFVEFYAPWCGHCKQLAPIWDKLGETYRDHENIVIAKMDSTANEVEAVKIHSFPTLKFFPAGSGRNVIDYNGERTLEGFKKFLESGGQDGAAADDDLEDLETDEETDLEEGDDDEQKIQKDEL"

     sig_peptide     36..101
                     /gene="P4HB"
                     /gene_synonym="PDI"
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
     mat_peptide     102..1580
                     /gene="P4HB"
                     /gene_synonym="PDI"
                     /product="Protein disulfide-isomerase.
                     /evidence=ECO:0000269|PubMed:2851999. /id=PRO_0000034200"
                     /note="propagated from UniProtKB/Swiss-Prot (P09102.3)"
     misc_feature    120..1472
                     /gene="P4HB"
                     /gene_synonym="PDI"
                     /note="protein disulfide isomerase, eukaryotic; Region:
                     ER_PDI_fam; TIGR01130"
                     /db_xref="CDD:273457"
     misc_feature    210..212
                     /gene="P4HB"
                     /gene_synonym="PDI"
                     /note="Contributes to redox potential value.
                     /evidence=ECO:0000250; propagated from
                     UniProtKB/Swiss-Prot (P09102.3); other site"
     misc_feature    213..215
                     /gene="P4HB"
                     /gene_synonym="PDI"
                     /note="Contributes to redox potential value.
                     /evidence=ECO:0000250; propagated from
                     UniProtKB/Swiss-Prot (P09102.3); other site"
     misc_feature    408..410
                     /gene="P4HB"
                     /gene_synonym="PDI"
                     /note="Lowers pKa of C-terminal Cys of first active site.
                     /evidence=ECO:0000250; propagated from
                     UniProtKB/Swiss-Prot (P09102.3); other site"
     misc_feature    1242..1244
                     /gene="P4HB"
                     /gene_synonym="PDI"
                     /note="Contributes to redox potential value.
                     /evidence=ECO:0000250; propagated from
                     UniProtKB/Swiss-Prot (P09102.3); other site"
     misc_feature    1245..1247
                     /gene="P4HB"
                     /gene_synonym="PDI"
                     /note="Contributes to redox potential value.
                     /evidence=ECO:0000250; propagated from
                     UniProtKB/Swiss-Prot (P09102.3); other site"
     misc_feature    1431..1433
                     /gene="P4HB"
                     /gene_synonym="PDI"
                     /note="Lowers pKa of C-terminal Cys of second active site.
                     /evidence=ECO:0000250; propagated from
                     UniProtKB/Swiss-Prot (P09102.3); other site"
     misc_feature    1464..1580
                     /gene="P4HB"
                     /gene_synonym="PDI"
                     /note="propagated from UniProtKB/Swiss-Prot (P09102.3);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    1569..1580
                     /gene="P4HB"
                     /gene_synonym="PDI"
                     /note="propagated from UniProtKB/Swiss-Prot (P09102.3);
                     Region: Prevents secretion from ER"
     exon            196..402
                     /gene="P4HB"
                     /gene_synonym="PDI"
                     /inference="alignment:Splign:2.1.0"
     exon            403..536
                     /gene="P4HB"
                     /gene_synonym="PDI"
                     /inference="alignment:Splign:2.1.0"
     exon            537..674
                     /gene="P4HB"
                     /gene_synonym="PDI"
                     /inference="alignment:Splign:2.1.0"
     exon            675..779
                     /gene="P4HB"
                     /gene_synonym="PDI"
                     /inference="alignment:Splign:2.1.0"
     exon            780..905
                     /gene="P4HB"
                     /gene_synonym="PDI"
                     /inference="alignment:Splign:2.1.0"
     exon            906..1106
                     /gene="P4HB"
                     /gene_synonym="PDI"
                     /inference="alignment:Splign:2.1.0"
     exon            1107..1227
                     /gene="P4HB"
                     /gene_synonym="PDI"
                     /inference="alignment:Splign:2.1.0"
     exon            1228..1409
                     /gene="P4HB"
                     /gene_synonym="PDI"
                     /inference="alignment:Splign:2.1.0"
     exon            1410..1496
                     /gene="P4HB"
                     /gene_synonym="PDI"
                     /inference="alignment:Splign:2.1.0"
     exon            1497..2544
                     /gene="P4HB"
                     /gene_synonym="PDI"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

acgcccgtcccggcccgtcccgtctcagcgccgccatggccgtggtgcggctgcgcgcgatcgtagcgctgctctgcctcgtgccgccgctcggcctcgcggagcccctggaggaggaggacggcgtcctggtgctgcgcgccgccaacttcgagcaggccctggcggcgcaccgccacctgctcgtggagttctacgcgccgtggtgcgggcactgcaaggcgttggccccggagtacgcgaaggcggcggcgcagctgaaggcggagggttccgagatccgcctggccaaggtggacgccacggaggaggcggagctggcgcagcagttcggcgtgcgcgggtaccccaccatcaagttcttccgcaacggggacaaggccgcgccccgcgagtacacagctggcagggaagcagatgatattgtcagctggctgaagaagcgcacgggaccggctgccactaccctgacagatgctgctgcagcagagacgttggtggattccagtgaagtggttgtgattggtttctttaaggatgtgacatcagatgctgcgaaggagtttttgttggcagcagaatccgtggatgacattccttttgggatttcttccagcgctgacgtcttctccaagtaccagcttagccaagatggagtggtccttttcaagaagtttgatgaaggacgtaacaactttgaaggggatcttaccaaagataacttgctgaacttcatcaagtctaatcagctgcctcttgtcatagagtttactgagcagactgctcctaaaatctttggaggagagatcaagactcacattctgctgttcttaccaaaaagtgtgtctgattatgaagggaaattggacaacttcaagactgcagctggaaacttcaaagggaagatcctgttcatcttcatagacagtgaccacagtgataatcaaaggattctggagttttttggtctaaagaaagaagaatgtccagctgtacgtttgataacattagaagaagagatgaccaaatacaaacctgagtcagatgacctcacagcagacaagatcaaagaattctgtaataaattcctggagggcaagatcaagcctcacctgatgagccaggatctccctgaagactgggacaagcagcctgtcaaagttcttgttgggaagaattttgaggaagttgcttttgatgagaataagaatgtctttgtggagttctatgccccctggtgtggtcactgtaagcagctggctccaatctgggacaagctgggagagacctacagagaccatgagaacattgtcattgccaagatggattcaacagccaatgaagtagaggcagtgaaaattcacagcttccccacgctcaagttcttccctgcaggctctggcagaaacgtaattgactataatggagagaggacactagaaggcttcaaaaaattcttggagagtggaggccaggatggagcagcagctgatgatgatctggaagatctggagacagatgaagaaacggaccttgaggaaggtgatgatgatgagcaaaaaatccaaaaggatgaattgtaaagagatgactgatctacatcacccaaaaatcagacactaaattggttgctgttacgcaacccaatgagtcagaaacccattaagatttaaagcagaaggtgaatgactggaaatccagggattggctcttaaagtaacgcttcgccctcacttgtctttacacttcactatcttttttctttcttttcacttttgagaagggatctatcactaggtagccagcccagccagctgggttcattattttttttattgtgatgtacttttttgtacacagttttgtttcaagtattttgttctcttgggttagaagcaagttgtgaagtggtagcatgaatttaattgcccaacattctgaagcattgccactaatatcttccaagaaagctttttctttctttttctttgaaaagcctgtagatttctccacctgtcagggttctcatttgtctcagctgttcttccctggaatcaaagtatgtaggggaagaggcaataggctgactgcatcagtcctggtgaaaggtggatgtgaaggtgcttctcttcatgctcgctagacatttcatttcctttcccttctgctttaagacacttaaagatgacttcaaaatgtggaagggtggaatactgtgcactggctatggcaggctctaagggtggaagtgattgctggctcaccagcttagatgtgctgccatccttttgatcaaatgcgagaccattttgcagtcacttcccatgcattagtttgaatgggttgaccaggaaggtgggtggtatgggagggcctgataaaaatacatgaaacgaaatttgcaattactgggtttgggggaggaacttgaacaagggagtgcctcaaaggcctgacctgcatcaggcctagttcttagcattccgcttacaataacattggaactgaatttgttggccaaataaagttgagattttaatacta

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