LOCUS       XM_009294368            9676 bp    mRNA    linear   VRT 12-MAY-2025
DEFINITION  PREDICTED: Danio rerio argonaute RISC catalytic component 2 (ago2),
            transcript variant X1, mRNA.
ACCESSION   XM_009294368
VERSION     XM_009294368.5
DBLINK      BioProject: PRJNA1248012
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_133194) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On May 12, 2025 this sequence version replaced XM_009294368.4.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_049306965.1-RS_2025_04
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 04/11/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9676
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /strain="Tuebingen"
                     /isolation_source="Zebrafish international resource
                     center"
                     /db_xref="taxon:7955"
                     /chromosome="19"
                     /sex="male"
                     /tissue_type="Fibroblasts and whole tissue"
                     /dev_stage="adult"
                     /ecotype="United States"
                     /geo_loc_name="USA"
                     /collection_date="2022-07-19"
                     /collected_by="National Human Genome Research Institute"
                     /genotype="Double heterozygous"
     gene            1..9676
                     /gene="ago2"
                     /note="argonaute RISC catalytic component 2; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 14686 long SRA reads, 12 Proteins"
                     /db_xref="GeneID:570630"
                     /db_xref="ZFIN:ZDB-GENE-110606-6"
     misc_feature    1
                     /gene="ago2"
                     /experiment="COORDINATES: cap analysis [ECO:0007248]"
                     /note="transcription start site"
     CDS             179..2875
                     /gene="ago2"
                     /codon_start=1
                     /product="protein argonaute-2 isoform X1"
                     /protein_id="XP_009292643.1"
                     /db_xref="GeneID:570630"
                     /db_xref="ZFIN:ZDB-GENE-110606-6"
                     /translation="

MYPIGAAGATELFQGRPSSGSDVSAPASPPAPQEYVFKPPQRPDFGTMGRTIKLQANFFEMEIPKLEVYHYEIDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVYDGRKNLYTAMPLPIGRDKVELEVTIPGEGKDRSFKVAIKWMSCVSLQALHEALSGRLPNIPFETIQALDVVMRHLPSMRYTPVGRSFFTPSEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFMCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQENGQTIECTVAQYFKDKYKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQDEISKLMRSANFNTDPYVREFGVMVRDDMTEVNGRVLQAPSILYGGRVRISSAQLSIWLNPCLIWSCLWLFQNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTELLLKAFTDQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFKHLKYTYQGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQVKNVQKTTPQTLSNLCLKINVKLGGVNNILLPQGRPLVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQQHRQDIIQDLATMVRELLIQFYKSTRFKPTRIIYYRDGISEGQFNQVLQHELLAIREACIKLEKDYQPGITFVVVQKRHHTRLFCMDRNERVGKSGNIPAGTTVDTKITHPFEFDFYLCSHAGIQGTSRPSHYHVLWDDNHFTSDELQVLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDQQALAKAVQIHQDTLRTMYFA"

     misc_feature    494..718
                     /gene="ago2"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    746..898
                     /gene="ago2"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    899..1261
                     /gene="ago2"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1055..1057,1100..1102,1142..1144,1154..1156,
                     1208..1210,1229..1231,1235..1237)
                     /gene="ago2"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1394..2746
                     /gene="ago2"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1880..1882,1892..1894,1928..1939,1946..1948,
                     1970..1972,1979..1981,1991..1993,2003..2005)
                     /gene="ago2"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2084..2086,2090..2092,2300..2302,2714..2716)
                     /gene="ago2"
                     /note="active site"
                     /db_xref="CDD:240015"
     polyA_site      9676
                     /gene="ago2"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

actcggagctctcgtgctccagcaactctgcgcgctcactttaatcttacttttcacctctgtatcgatgctgttttgaaagaaaacccagctcttccgttaccgcatgtgttacgtggcatcagaagacccaaaagtgtcggcccgggattgaattttgctgcagaaagcgattaaaatgtatcccattggagcagctggtgctactgagctgtttcagggacgtccgtcttcagggtcagatgtttctgctcccgcgtctcctccagcaccacaagaatatgtcttcaaaccacctcagcggccagactttggcacaatgggtaggacaatcaagcttcaggccaacttctttgaaatggaaattcccaagttggaggtttatcattatgaaatagacatcaagcctgagaaatgcccacggagagttaaccgtgaaatagttgagcacatggtccagcactttaaaacgcagatctttggagatcgaaagcctgtgtatgatgggaggaagaatctctacactgccatgcctttacccattggaagagacaaagtggagctggaggtcacgattcctggggagggaaaagacaggagctttaaagtcgctataaagtggatgtcctgtgtcagtctgcaagctttgcatgaagcactctctggacggctaccaaacatccctttcgagaccattcaggctctggatgttgtcatgagacatttgccctctatgaggtacaccccagtcggacgttcattcttcactccatcggagggctgctctaaccctcttggtggaggaagagaagtttggtttggttttcatcagtctgttcggccatcactctggaagatgatgctcaacattgatgtgtctgccactgcgttctacaaagctcaacccgtcattgaattcatgtgtgaggttttggattttaaaagcatcgaagagcagcaaaagcccttaacagattcccaaagagtgaagtttaccaaggagatcaagggtttgaaggttgaaattactcactgtggacaaatgaagaggaagtacagagtgtgtaatgtgacgagaagaccagccagccatcaaacgtttcctttgcagcaagagaatggtcagaccattgaatgcactgtcgcccagtacttcaaggataagtacaaactggtgcttcgatacccacatctcccatgtttacaagttggtcaggagcagaaacacacctaccttccgttggaggtctgcaacattgtagccgggcagcgatgcatcaagaaactgacagacaatcagacctccactatgatacgtgccacagccaggtctgcgcccgaccgtcaggacgagatcagcaaactgatgagaagtgccaacttcaatacagatccttatgtgcgtgagtttggagtcatggtaagagacgatatgactgaagtcaatggtcgggttcttcaagcaccgtcgattctctatggtggaagggtaagaatctcctctgctcaactcagcatttggttgaatccctgtctaatttggtcatgtctttggctgtttcagaacaaagcaatagcaaccccagtccagggcgtgtgggacatgaggaacaagcagttccacacagggattgaaatcaaagtttgggccattgcctgctttgccccacagaggcagtgcacagaactcctgctgaaggccttcactgaccagcttcgtaagatctcacgtgatgctgggatgcccattcagggccagccgtgcttttgcaaatatgcccagggagcagacagtgtggagcccatgttcaaacacctcaagtacacttatcaagggttacagttggtggtggtcatcttacccggcaagactcctgtttatgctgaggtgaagcgtgttggagacactgttctcggcatggccacgcagtgtgtccaggtgaagaatgtgcagaagaccacacctcaaaccctttctaacctctgcctcaagattaacgtcaaactgggtggagtcaacaacattcttcttccacagggcaggcccttggtgttccagcaaccagttatctttttgggtgcagatgtgactcatccaccagctggagatggcaagaaaccctcaattgctgctgtcgttggcagtatggatgcccacccaagcagatactgtgccacagtgcgggtgcagcagcaccggcaggacatcattcaggatctggcaaccatggtcagagagttactcatccagttctacaaatccacacgcttcaaaccaacacgcatcatctactacagagacggcatctctgaaggccagtttaaccaggttctacagcatgagctgctggctattcgtgaggcctgcatcaagctggagaaagattatcagccgggtatcaccttcgttgtagtacagaagagacaccacaccagactcttctgcatggacaggaacgaacgggttggaaagagtggaaacatccctgctggcaccactgtcgacaccaaaattactcatccattcgagtttgacttttatctttgcagtcatgctggaattcagggaaccagtaggccgtcccactatcatgtgctgtgggacgacaaccatttcacctctgacgagctgcaggtcctcacctaccagctgtgccacacctatgttcgctgcacccgctcggtctccattcctgcaccagcctattacgcccacttggtggcgtttcgcgcccgctaccacttggttgacaaagaacatgacagtgctgaaggcagtcacacatcaggtcagagtaacggcagagatcaacaagccctggccaaagctgtgcagatccaccaggacacactgcgcaccatgtacttcgcctgaacaaagccagaaccaagccccacaatcctgaaaacagcacggtgtgtgtgtatatgtgtgtgtgtgtgtgcgtgtgtgtaattttaagtgttatacaaacgagacccttcaaatggagacaaaatgaggaccgccatgctgttcgacttttaactgccccttaattcggtttccaaacatatttaatatccaagctgatatgtgaatttttattcatttatcttttaattttgttcctcgtttggttcagtgggcatggttgtgtatctcttgtgcatgtcagattagaggttaacgcgtttacatagtcaatcccaaaaaattctgactttttttacactgaaagttacatttgcatccaagcgggcttgatttagtactattgccaaggcaggtgatggagactgttgaacattagtttttacaaactcatcaaaccaatggattcgggtcagtttaggacttcacggatcaggtttagggcttgtggcgatgcagtttccacagctaaattatttatcagcgaacgcaagtgcactgaccagaaggggacttcaggacaaaagtacttatcctgttttatttcatctatttgtgctaatatgatttattttcttcttctagtggtgtgatttcttgttcagtccagggctcagtcgacctactgtgtacctgtccgccaatgatcatgtgtcagtacagaatcatgaacgcatttggatgtaggaattgttttgacaatcagttttctgccgctcatatgagactggttctcattcgtttttcctgtgccgggttctcagtgcggaaacgactctgtactgtggatagatgtcctgaaaagtactattatcttgcattaagatgagattatctttcaattgtactgccattttcgctggtttatccgccgtgattgtaacaacctcaagtttcggtcgtttgtgacatcaaaatttaatcacatttcactttaagcacctattcgttgttcataatgttccccaaattgaaacttgaggtgacttttgtcagtaatattagggaaagtggagccctgtgatttttgcttggttctttgtgctgttttcagtggtgaagtttgactgtgttttgctgacagggtcgtcgtcgcactccagcaggcaatcaaaacttttaaagaacgataccaatatcagaatttttcacacaacgaagtcagaatgaaaatttcaaatgcaccttagattgtttgtttttttccctaactcgcgtgttcccatgatgagaatttttcgtatctctttccaacactcaagatagtttctaaatatttaatctttctttcatagacctttcatagagtcatgaggcgagcgctgaggcgtttataatgaatcgaaaacatttatttagtgtaaatcagtccaaatggagcttgaattgaagcgcaatacgttgtttgtttttgtgtgtgtgtgtgtgtgtcggtgaactgatttcttgtaaacagccttctcaagcatatcatgatgtgtagtggtcgatctatcgagtctcgttagcgaattaaccgtaagatcacttccccaaataatgcttcaattctgaacttgaaattcttgggaatatttttaaccatctttttttctaactatgcattcttaaccaagagattctagtggtatgtttgtgtatatgaattatgaatttagaacgtttgtaatgtagtccttttcatgccgggtacttttagtagtgtaaatagttccagccctgtacccgctgtagcagtattagcttacaaaatactctatttttattaacaattggatgttctttatatggctctcaattattcctgttagccaaacaagattactatgtacttgtagtcggtgtcctaccataactgaagcacaataggacattataattgtaggtgctagagtttattcttgagactaccatttgttttttttatcgtcaccttttaaacgagaagcatggagtgcttgtttgttttgtttattttattatttttatctatgacatttaacccaacccaacagactgtgtataaggggcattttaatttatcttccaatacttgactagagttctgttattatttggcgctgatcttgtaactcgtgaaatttagatgcactttttttttttttttttttttttttttttgagagcaatggatacatccctagtttaccttcggtggctaaatggattttgagtatgctaactgtgtgcagttgtgtggaaatgactagatgtcctacctcttgttacctgttacttgcattatggtaacattaccgcactggtcatttcctcataattgcattaagtggaagttgatgatcttgctctaaagtggcgtctttgttaattcatgttttgtttcagaggccatacctgctgccatttagcctagacgcttattgttttattggtagttttaagctatgtattcaaagctacgattgagtgctgcgagtttcaaatgtcgtacgtttgatgttttgaagtcagacgtgaatccaaaaagaatattaaatgcttgggtgtgcaccatcagatgttcctaaaatgtgtagtgtggattcaagttgattgctttctgccctttataattcgcagcgcgttgttgctttatccatgtactcgtttaaatgtgtcacgtagttttcctgtatctttttttttttaaggtttgaataatattatttatcttttagcatcagaaacatgagatgaaatcatcgctcaaactactttttacatcgatttttctcttgaacaatcagatttttatttcttcgaaaccattatatttcattctcatgaactgctctgtttagttgttttcagaagttactgagtttttagtatttgaacctgtttgaaatcagcaccttagtcaaaaggtctacaacatatgcaatttctcaagtatgaaatatgagatgtttttgcactatatcagaagaggtgttaataatttgtggaaggttagagaggagaagaaccatcaagtttagactagtcttggcctcgtttctttgttcatgtacaatcatattggcactaacctattcaaagttgacacattagaaggtcttgagttcttacgctatgcaaaaactcatttccatttagagccgtcgctctttttatacatggtgttcttgcagaacaagttgcgtttaccaaattgagtttgggaatttgagtaaactcaaatcccattttatatgttttccaaacctgttttctgtttaatgaggacatgagtctgatgcaaattttttatcgaaataattccgttaaatgcaaatggccaaggacagagggagaagaatgaagtaaggtgctggttttgtagagaccgtcacgataatctatgctgcagcatctctgtttcagatcttaacaggcacatatagtgttaactgaaaattgaattggtgcctctgaaagctttgaattgtttatccgtcaatgttacgccattaacattttatggatcgctctttcatcatcatttctagattgctgcttgagtgaagcttgtatattgctttggcgaggttctgtgtggggttacagtgtccacatgcttctttgttttacactttattgtttgaataaaactagttgattgccccaataaatattatttaaaaaccataaacgccagtctcatcaaaggttagagcccctaatcttaaaagatatatttgctaatgtattggcattagaactttgcactagtgttctgaagcagaaatgtacgcctctcttcacataccgtgatcgttttgtaattcctaaggtggcaagtttcaaagatgccagccttatgacctgctacaaccatacggatacaagcgaacagtttgaatgtacttcatacgcctgcatactggttaagaacctccattattttaatccgtttgcttgttttgttttatgcatgcacattgaaagcagctttgagtcgttgaactttttatccatgcatactttgaactgtatctcaaaactcatcgttgctttaaattaatagtggcaacatttggacggatggttcagaatacacactggcacaaagatctgatgtttgtggtgctgtactgtaaaaagtgtacataaggcagcgttttctgtgtgtttttttgcctattactgctcttgtttgacagattagaagtttcatctaaagggtttttgagctatacaacaatacccatgtacccaacaagccaaattcacactcgtatgaagcccttttgctttaccagctctgtggtggtcctgtaatcatggcagtggtacttgtgcacaaactaaaccacagatcagttgccatttagctagttgtctgattggggaatgatggtttccgaacatgtcccctttactatttttaaaatgaatctaaatgaccaaaatttttgcaagggattaatttttttttccaaaacgagcacagttccattaattcattataaatcatgctaaaatggtatttattcgaatataagcatcacagttgggtcttgtgactgattcatctgaataactgagagcttcatcatgacctggctttttaaaccaacttggagaagaaaaaagaaaaaaaaaactacctcaatagtgtcgggtgctaatgttaaaatatcccttatatgtatatgtgctcatgacaaagaacaataatcgtgaccaaacaacacaagcctatactgtgtattacgtgtttagtttttattatttgtgtatgatggatcaacatgagggatgtaacaaaactgcggtggaccaaaataaatcgtttccctttttaaaatgtagttttcaatatgcatcagttttattcgaattaaatggagctgttctcagatcctctggagaacaaatggtcaagaatgagcgttcaggccaagattgttgccaaaacacggtttcgttttgtcagacgtcgtgttgagttggttgatctagaattgtgccatattatggagaacatacaggacagtaaacgtccaatgaccactgctctgtgtctaaagcgtgttcctattaagcggcttgtcaacctgtgtcagcttttggagtgctagcgttatacttgattcttctacttgatttgtatccagtattgcaataaaaacttcgcactcatccgatccggaactgttcgatgaaagctccacggcgttttttttgtttttgtttttaagctatctgtatttaaactgtcatgtgacgtcgctacattttatgaagggaatttttaatttgacttctctcaaactctgattaaggacgcgttggggcgtcctgatacactggcatttgagtttctcgagggagagcataataaggtacaaaaggttgtgtggaaatcagttacgattctcgtttctcattggtgagctcaataacgtccagcgtgtttttatgtcgtgtttgtgtgcctttggaaatctctcccgaccctgatcaagggactgtcctttattcatttgatgctcggcgccacatcatttatcatgcaaccttgagtgaatgctgcatagaatgatcagtatttgtattttgcactttctaagcagttttaaaggacgccctgttgtaattggggcgcacagactatttaagacgaggtattttctgctacaataggaattttaaggaaccatatgactcgttggttgaaaaaaaataaagtgtatagatgtgaagtacctcataagcctgataaatgctgcataaactcttcatttattgttcttttgtgaatctattttcccctattggaaggtttgatgtgcacaacagcgcaaagcaccttcatttatgacattgaggcgctctaaaaccagttatgcaagtcaaatgcgaggttttcggtcatattcactgacattataagacattagcaagcctctagcttcagctttgcatcacagctattggatacgtagtgacgtttgcagtcaaaagttacagttgactcaggtatcgccattaagggctgtatctgcttttgcctcccattttccctcacctgtgtcttaacgtagccttagaactgcattcatggatggatagatcatagtgaagaatatctggccttaaaaatatgcactatcaaccacaacatgcaagtcactacagatcagatctttcgttttctgccataaaactacctccgagaatgtaggagtgtcctggaactgacacatcaaggtttattttcatttttaaggttataatctgtaatcgacaatcatcttttagtttattaaattcgcagtatgagttacagttcattctgggacgctctagttttgtttctttatgaaaagcgtgtccatgttagtcatggaagagccaagcgaccaacgtttctagctgaaggttttcttgcactgctttgtgacgtcaaaccttcctctttttttttttttacatcagcaaattatctttcatttaacctcgtgttttgctcctttataacatgccaaattaaatttgctgtcattcctgtcatggtaaagtctttaatttctgattcatgtgtttgcattcactgctaagctctatgttgttgtttcttggtaatgtaaagtgagtttatttctttttttttttttttttttaacaagtaagattacaaccaagatatgctgtaaaccctgctaagacataatgtgaaaacgtatcatttaatatagctcacttttgcttgactgtgtattgcatcatttaataaagttaatttcctcttaa

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