LOCUS       XM_009292707            5450 bp    mRNA    linear   VRT 12-MAY-2025
DEFINITION  PREDICTED: Danio rerio argonaute RISC catalytic component 3a
            (ago3a), transcript variant X3, mRNA.
ACCESSION   XM_009292707
VERSION     XM_009292707.5
DBLINK      BioProject: PRJNA1248012
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_133191) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On May 12, 2025 this sequence version replaced XM_009292707.4.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_049306965.1-RS_2025_04
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 04/11/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /strain="Tuebingen"
                     /isolation_source="Zebrafish international resource
                     center"
                     /db_xref="taxon:7955"
                     /chromosome="16"
                     /sex="male"
                     /tissue_type="Fibroblasts and whole tissue"
                     /dev_stage="adult"
                     /ecotype="United States"
                     /geo_loc_name="USA"
                     /collection_date="2022-07-19"
                     /collected_by="National Human Genome Research Institute"
                     /genotype="Double heterozygous"
     gene            1..5450
                     /gene="ago3a"
                     /gene_synonym="eif2c1"
                     /note="argonaute RISC catalytic component 3a; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 2523 long SRA reads, 52 Proteins"
                     /db_xref="GeneID:567622"
                     /db_xref="ZFIN:ZDB-GENE-051107-16"
     misc_feature    1
                     /gene="ago3a"
                     /gene_synonym="eif2c1"
                     /experiment="COORDINATES: cap analysis [ECO:0007248]"
                     /note="transcription start site"
     CDS             655..3237
                     /gene="ago3a"
                     /gene_synonym="eif2c1"
                     /codon_start=1
                     /product="protein argonaute-3 isoform X3"
                     /protein_id="XP_009290982.1"
                     /db_xref="GeneID:567622"
                     /db_xref="ZFIN:ZDB-GENE-051107-16"
                     /translation="

MEIGTTGAVGAQSLFSMPRRPGYGTMGKPIKLLANCFQVEIPKMDVYLYEVDIKPEKCPRRVNREVVDSMVQHFKVTIFGDRRPVYDGKKSLYTANPLPVAPAGVDLDVTLPGEGGKDRPFKVSIKFVSLVSWHLLHEVLTGRSMPEPLELDKPISTNPVHAVDVVLRHLPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYEADPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEVIQDLSSMVRELLIQFYKSTRYKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKEYQPGITYIVVQKRHHTRLFCADRNERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADEFQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTLRTMYFA"

     misc_feature    871..1155
                     /gene="ago3a"
                     /gene_synonym="eif2c1"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    1183..1335
                     /gene="ago3a"
                     /gene_synonym="eif2c1"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    1336..1698
                     /gene="ago3a"
                     /gene_synonym="eif2c1"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1492..1494,1537..1539,1579..1581,1591..1593,
                     1645..1647,1666..1668,1672..1674)
                     /gene="ago3a"
                     /gene_synonym="eif2c1"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1831..3108
                     /gene="ago3a"
                     /gene_synonym="eif2c1"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(2242..2244,2254..2256,2290..2301,2308..2310,
                     2332..2334,2341..2343,2353..2355,2365..2367)
                     /gene="ago3a"
                     /gene_synonym="eif2c1"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2446..2448,2452..2454,2662..2664,3076..3078)
                     /gene="ago3a"
                     /gene_synonym="eif2c1"
                     /note="active site"
                     /db_xref="CDD:240015"
     polyA_site      5450
                     /gene="ago3a"
                     /gene_synonym="eif2c1"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

gagtgtttgagaaagcgggcagagagagagttctgcttccaacttactttatgtgaccactggcggaaaaccacgggaatacgcgcttataaaaagccactagagccgagagcgaaattcatggcagaccggggactttaagcgaagacacgactggatttttgtttcctctcagatgggaatgtagcttagctggttagcctggccactagcagtagcaagttagctctgtcgaagagcggaggcgacataagtggacttttacttgctataacgtcataacacctctacaaaccgccctagtctttccaaagacgtgtttctgaaggcgtcgccgtcacagaaacagagactcgggactgaatttgggctgactgagaggaacttaccgttaccgttttagctagctggctagctggccgtcacgtcaatctgtcaaccgtacccgaaatcccgaccggatagtttgagtcacataccgggcaaccaaacattatctccggtgacacacgagcctcagggaaggtggactaataccgagaggaatcgcttgcggtgggttaacgcaaccggtttgtgcggccataggacagtcagagtttctgaagttcatcaactgcgagccggggacggaaccggacagagacctcatgaatggaaatcggaactacaggagccgttggggcccagtccctgttctccatgccacggaggcccggctatggcaccatggggaaacctatcaaactgctggccaactgcttccaggtggagatcccaaagatggacgtctacctgtacgaggtggacatcaagcccgagaagtgtccacggagagtaaatagggaggtggtggactccatggtgcagcactttaaagtcaccatctttggggatcgaaggccagtttatgatgggaagaaaagtctctacaccgccaacccacttcctgtggcacctgcaggggtggatctggatgtcacgctgccaggtgaagggggcaaagaccggccctttaaagtctcaattaagtttgtgtctctggtgagctggcacctgctgcacgaggtgctaaccgggcgcagtatgccagagccgctggaactggacaagccaatcagcaccaacccagtgcacgctgtggatgtggtgctccgccatctgccctccatgaagtacacacccgtgggccgctcatttttctccgccccggagggttatgatcatcctctaggtggaggcagagaggtgtggttcggtttccatcagtctgttcggcccgccatgtggaaaatgatgctcaacattgatgtgtcagccacggctttctacaaagcacagccagtcattcagttcatgtgtgaagtcctggacatccacaacatcgacgagcagccgcgacccctcacagactcccacagagtcaaattcaccaaagaaatcaaaggtctgaaggtggaggtgacccactgcgggaccatgcggaggaagtaccgcgtctgcaatgtgacccgccggcctgccagccaccaaacgttccctttgcagttggagaacggccaaactgtagaacggacagtagctcagtatttcagagagaagtacaacctgcagctcaagtaccctcacctgccctgtctacaggtgggacaggagcagaaacacacatacctgcctctggaggtttgcaatattgtagcagggcagcggtgcatcaagaaactgacagataatcagacgtccaccatgatcaaagccacggcgcgctccgcaccagaccgacaggaggagatcagcagactggtgcgcagcgctaactatgaagccgacccgttcgtgcaggagtttcagtttaaggtccgggatgagatggcccacgtgacggggcgcgtgttgcccgcgcccatgctgcagtacgggggcaggaaccgcactgtcgccacaccgagtcacggcgtttgggacatgagaggaaagcagtttcacaccggcgtggagatcaagatgtgggccatcgcctgcttcgccacacagaggcagtgcagagaggagatcctcaagggttttaccgaccagctgcgcaaaatctcgaaggatgctgggatgccgattcagggtcagccgtgtttctgtaagtacgctcagggggccgacagcgtggagcccatgttcagacacctgaagaacacttactctggactgcagctcatcatcgtcatcctgcctggaaaaacccccgtctacgcggaagtgaagcgtgttggagacactcttctaggaatggccactcagtgtgtgcaggtgaagaacgtggtaaaaacctccccgcagacgctctccaacctctgcctgaaaatcaacgtcaaactcggcggcatcaacaacatcctcgtgccgcatcaacggccatcagttttccagcagccagtcatctttctgggggcggacgttacacatccccctgcaggagatgggaagaaaccgtccatcgcagcggtggtgggcagtatggacgctcatcccagcaggtattgtgccactgtgcgcgtccagagaccccgtcaggaggtcattcaggatttgtcctcaatggtgcgagagttactcatccagttctacaaatccactcgctacaagcccaccagaatcatcttctacagggacggtgtttctgagggccagttcagacaggtgttgtattatgagctgctggccattcgagaggcctgtatcagtctggagaaggagtaccagcccggcatcacgtatatagtggtgcagaaacgccaccacacacgcctcttctgtgccgaccgcaacgagagggtgggtcgcagtggaaacattcctgctggtacgacggtggatacagatatcacacacccttatgagtttgacttttacctctgcagtcatgctggaatacagggcaccagcaggccctcccactatcatgtcctgtgggacgacaactgcttcactgctgatgagttccagctgctcacctaccagctgtgccacacgtatgtgcgctgcacccgctccgtctccatccccgcgccagcctactacgcccacctggtggccttccgcgcccgataccacttggtggacaaagaacacgacagtgccgagggaagccatgtctccggacagagcaacggccgggacccccaagcgctggccaaagctgtgcagattcaccacgacaccctgaggaccatgtactttgcctaagccagctcacgttcatctagtcatgcacacaaacccgacaaccacacacacacacacatgctgacaaacacacacacctcccagaatcctgtgcgtcctgaagggtcgatctctgtggcgattgaagcacggcccacccgacactgtgcagcaggacgaggaaggcctttaaggaagccgacctgctttaaaaccacacatggacctcggcgctattatgcaatattaaaccagccaactgaggccgttctgtcagatccccctccaaaaccaaaagggaagtccgtctgtatatccctcccgctccttttcctgtctatagacgtcacgttttaatcacttccccccccctgcctgtcacgccttgtacctcaacgtcttttctaacagcactttcccctccccccgtttctctcttaatgtacccccacccccccccccccccmcccccccccccccccccccccacccccccaaaatgagattcgaatggaagaaaagactgagtccggaagttttgggaagttttaaggcatcacatgctcttactactactactactatgtacttttctccccttctcgattatttttgtagccaatggtgacttaactacgtgttgaaagtgtccgttgtgaagaaccaatcgtgcgttagaattttaaagcagaattattgttagaaaaagtgccattgccgagcgtaaagtcctttatgacgtacccccacagtggggaagaaagctgaccgttagcatattttacgctccgtaatatctgtgagctttaattgtacctatgacgcaagtgatcacgttttgatatgctttttcaaacggccgaaattccctccaatatttattggtctcgatcaaacgcagctcctctgtcgtgttcacttcctttcagcacttttgtctcgatttaaacaaacaaatgcatagtccgtgttgtgtgacagatcatagtttagtgcaataatccattaagcgggatgagaatacctccagtgcaaagactcacggtttgctctttggggaagagggagagcaagagaatttaaaaggcagccgaatgcgtgattggtgggtttttaggatggaggtgtgaattgttggcacttttatagtttatttttcgaacgttggacgatcgatggcgtgttaacaagtgcctgcttgaaatgcttgacgttgagcagttgaaagtgtagttcaacccgaaaggcaccacattcgactgtacggccttaataaaatctcgagccagagcgctttcaactctcgcttttagtttcgtttctcactgccctgcctgttcagaggatttcgctgctattgtacttgttggagtcagactggcgggtcatgcttcagcacgagggactgatcggcagcttgtgtgtgtgttttatgtggagatgctctcagcacctgatgtgtgctcgttcactggagggccactaggggtcagtatagtgttttacttgaagagtgcatctcggtgcagtgagcgttgggtgccttaccgtctctctgtgcaccatctgaagcatgtccagctgtttaaaggtgataatctgcactaaataatgcgtaatgcatggtgctattaggtgcggcccatggcggcatcagcaattggttatttgccggagacttaatttgtaagatgggaacatgggatgcataaatggtgctgggtgcatgtatttctcacattctcgtcaggacagacatttggtcgtttttgtatgtttgcatttgattttattatgttgaaaggtacagcactgtggccagtcggagtatgattatggttgaatgaagtgcgggatgttttaatgcataattaggatttttttagcctcttttaaatccaaacttttgcagctcaactctctacacagcaaaactgcaattggcttgtaaattgtgggctccttgtctcaatctagacatatacagttgaagatggaattattaatattaattagccctcctgttcgattgtaattcattttcaattatttcccaagtgctttttaaactgattcccccctccccccccccattattatattaaattattttattaagaaattttataaactctgctgggcgaggcagtggcgcagtaggtagtgctgtcgcctcacagcaagaaggccgctggttcgagcctcggctcagttggcgtttctgtgtggagttt

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