LOCUS       NM_131044               2901 bp    mRNA    linear   VRT 23-MAR-2025
DEFINITION  Danio rerio engrailed homeobox 2a (en2a), mRNA.
ACCESSION   NM_131044 NM_001128823
VERSION     NM_131044.2
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Maekawa,M., Saito,S., Isobe,D., Takemoto,K., Miura,Y., Dobashi,Y.
            and Yamasu,K.
  TITLE     The Oct4-related PouV gene, pou5f3, mediates isthmus development in
            zebrafish by directly and dynamically regulating pax2a
  JOURNAL   Cells Dev 179, 203933 (2024)
   PUBMED   38908828
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Altburger,C., Holzhauser,J. and Driever,W.
  TITLE     CRISPR/Cas9-based QF2 knock-in at the tyrosine hydroxylase (th)
            locus reveals novel th-expressing neuron populations in the
            zebrafish mid- and hindbrain
  JOURNAL   Front Neuroanat 17, 1196868 (2023)
   PUBMED   37603776
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2901)
  AUTHORS   Baldera,D., Baxendale,S., van Hateren,N.J., Marzo,M.,
            Glendenning,E., Geng,F.S., Yokoya,K., Knight,R.D. and
            Whitfield,T.T.
  TITLE     Enhancer trap lines with GFP driven by smad6b and frizzled1
            regulatory sequences for the study of epithelial morphogenesis in
            the developing zebrafish inner ear
  JOURNAL   J Anat 243 (1), 78-89 (2023)
   PUBMED   36748120
REFERENCE   4  (bases 1 to 2901)
  AUTHORS   Ulhaq,Z.S., Ogino,Y. and Tse,W.K.F.
  TITLE     FGF8 rescues motor deficits in zebrafish model of limb-girdle
            muscular dystrophy R18
  JOURNAL   Biochem Biophys Res Commun 652, 76-83 (2023)
   PUBMED   36827861
REFERENCE   5  (bases 1 to 2901)
  AUTHORS   Zhou,J., Yang,Y.J., Gan,R.H., Wang,Y., Li,Z., Zhang,X.J., Gui,J.F.
            and Zhou,L.
  TITLE     Foxl2a and Foxl2b are involved in midbrain-hindbrain boundary
            development in zebrafish
  JOURNAL   Gene Expr Patterns 46, 119286 (2022)
   PUBMED   36341978
REFERENCE   6  (bases 1 to 2901)
  AUTHORS   Fritz,A., Rozowski,M., Walker,C. and Westerfield,M.
  TITLE     Identification of selected gamma-ray induced deficiencies in
            zebrafish using multiplex polymerase chain reaction
  JOURNAL   Genetics 144 (4), 1735-1745 (1996)
   PUBMED   8978059
REFERENCE   7  (bases 1 to 2901)
  AUTHORS   Krauss,S., Maden,M., Holder,N. and Wilson,S.W.
  TITLE     Zebrafish pax[b] is involved in the formation of the
            midbrain-hindbrain boundary
  JOURNAL   Nature 360 (6399), 87-89 (1992)
   PUBMED   1436081
REFERENCE   8  (bases 1 to 2901)
  AUTHORS   Puschel,A.W., Gruss,P. and Westerfield,M.
  TITLE     Sequence and expression pattern of pax-6 are highly conserved
            between zebrafish and mice
  JOURNAL   Development 114 (3), 643-651 (1992)
   PUBMED   1352238
REFERENCE   9  (bases 1 to 2901)
  AUTHORS   Hatta,K., Bremiller,R., Westerfield,M. and Kimmel,C.B.
  TITLE     Diversity of expression of engrailed-like antigens in zebrafish
  JOURNAL   Development 112 (3), 821-832 (1991)
   PUBMED   1682127
REFERENCE   10 (bases 1 to 2901)
  AUTHORS   Holland,P.W. and Williams,N.A.
  TITLE     Conservation of engrailed-like homeobox sequences during vertebrate
            evolution
  JOURNAL   FEBS Lett 277 (1-2), 250-252 (1990)
   PUBMED   1980115
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CU041319.16.
            
            On or before Jun 6, 2012 this sequence version replaced
            NM_001128823.1, NM_131044.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X68151.1, X68446.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA3505372, SAMEA4476745
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-798               CU041319.16        1111-1908
            799-2901            CU041319.16        3555-5657
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /strain="Tuebingen"
                     /db_xref="taxon:7955"
                     /chromosome="7"
                     /map="7"
     gene            1..2901
                     /gene="en2a"
                     /gene_synonym="ENG-2; eng2; eng2a; Zf-en; ZF-EN-2"
                     /note="engrailed homeobox 2a"
                     /db_xref="GeneID:30243"
                     /db_xref="ZFIN:ZDB-GENE-980526-167"
     exon            1..798
                     /gene="en2a"
                     /gene_synonym="ENG-2; eng2; eng2a; Zf-en; ZF-EN-2"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    279..281
                     /gene="en2a"
                     /gene_synonym="ENG-2; eng2; eng2a; Zf-en; ZF-EN-2"
                     /note="upstream in-frame stop codon"
     CDS             318..1115
                     /gene="en2a"
                     /gene_synonym="ENG-2; eng2; eng2a; Zf-en; ZF-EN-2"
                     /note="homeobox protein en-2a; engrailed 2a"
                     /codon_start=1
                     /product="homeobox protein engrailed-2a"
                     /protein_id="NP_571119.2"
                     /db_xref="GeneID:30243"
                     /db_xref="ZFIN:ZDB-GENE-980526-167"
                     /translation="

MDENEQSARDVEQRGASDESNSAIRPLLQAPGNLQLPHRITNFFIDNILRPDFGRKKEANITRYEDNHGARENHNPTGPSTGQVGSTVPAEEASTTHTSSGGKEAEIESEEPLKPRGENVDQCLGSESDSSQSNSNGQTGQGMLWPAWVYCTRYSDRPSSGPRSRKPKKKAASKEDKRPRTAFTAEQLQRLKAEFQTNRYLTEQRRQSLAQELGLNESQIKIWFQNKRAKIKKASGVKNGLAIHLMAQGLYNHSTTSKEDKSDSD"

     misc_feature    318..407
                     /gene="en2a"
                     /gene_synonym="ENG-2; eng2; eng2a; Zf-en; ZF-EN-2"
                     /note="propagated from UniProtKB/Swiss-Prot (P09015.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    510..743
                     /gene="en2a"
                     /gene_synonym="ENG-2; eng2; eng2a; Zf-en; ZF-EN-2"
                     /note="propagated from UniProtKB/Swiss-Prot (P09015.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    780..857
                     /gene="en2a"
                     /gene_synonym="ENG-2; eng2; eng2a; Zf-en; ZF-EN-2"
                     /note="propagated from UniProtKB/Swiss-Prot (P09015.2);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     misc_feature    846..1016
                     /gene="en2a"
                     /gene_synonym="ENG-2; eng2; eng2a; Zf-en; ZF-EN-2"
                     /note="Region: Homeodomain; pfam00046"
                     /db_xref="CDD:459649"
     misc_feature    1017..1109
                     /gene="en2a"
                     /gene_synonym="ENG-2; eng2; eng2a; Zf-en; ZF-EN-2"
                     /note="Engrailed homeobox C-terminal signature domain;
                     Region: Engrail_1_C_sig; pfam10525"
                     /db_xref="CDD:431338"
     exon            799..2901
                     /gene="en2a"
                     /gene_synonym="ENG-2; eng2; eng2a; Zf-en; ZF-EN-2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

cagagtagccactgaagcctcgctagttgcactgcgcagcaaaccagtagcaccaaactggcaagttacggttcatcaaccttaaactgcaaacattccgtgtttggaaggcgtaactatgtttttcctttgtgaccacggtgcgcaaaggctttttactgtcgctaaaactcactgacagttttttggccgttttgaattttttttttcctgaagtgaacaaagtggacggagtcgcccgtggagtggggtgcgactgccagagtgcgggagagcgataaagggttaaggacggaaggtggaaagtgcgtctcagcatggatgagaatgagcagagcgcaagagacgtggaacagcgaggagcgtcggacgagtccaacagtgccatacgaccccttttacaagctccaggaaacctgcagctgccacaccgaatcacaaacttcttcatcgacaacatcctgcgaccagattttggccgtaaaaaagaagcgaatattacgcgctatgaggacaatcacggcgcacgagagaatcacaaccctacgggtccaagcacgggacaggtgggaagtactgtaccagcggaagaagcttccacaacgcatacgagcagcggaggaaaggaggcagagatagagagcgaagaacccctgaagccccgcggggagaatgtggatcagtgcttgggttcagaatcggatagttcacagagcaattcaaacggacagactggtcagggtatgctgtggcccgcttgggtttactgcacacgctactcggacaggccgtcgtcaggtcccaggtctcgtaaaccaaagaagaaagccgcaagtaaagaagacaaacgaccacgcacggccttcacggcggagcagcttcagagactcaaggccgagttccagaccaaccgctacctgaccgagcagcggcggcaaagcctggcgcaggaactgggcctcaacgaatctcagatcaaaatctggttccaaaacaagcgggccaaaatcaaaaaggccagcggcgtcaagaacggtctggcaatacacctgatggcacagggactgtacaaccacagcaccacgtcaaaggaggacaaatcagacagtgactgaggcagagagagcggggagggggaagagatgggtggggtggaccgggtggggggcaaggttatatttacaatgcaataattcaaaagaataaagggccagttaataaatataccagcattactgacgttaaaatagaggctatatcgaattatgtcaaagtacatttattgaacacaattctcttatgaaataatggtcaaattatttcaaaaataggctatacgtgccgttgtttgcacttggatagagtgtctatgttagaaacaagtgtttctatcgctctttgtcatgtcaccagaaccaagcgctattagcgcaacgggcgtttatatatacatttatgtcagtttatgtgagaaaaaaaacattgcgcgaaaccttgcttaagtccaaagagtcttttctgctgcatccaggcaactgtgagtactatgaaatgcgttagacttgttttttccacgtttttcgtattatttggtttgatgtttgatacgaaatggacgcagcaatcgttcggatctcagcaggattttttaacaaacagaaaaaaggaaactgagaagaaacactgtcctttttattgtaaataagttcacagtcttagcctggctaatttaaaagagtgaaatggaggaggaagaaatcacaaaacttgtttaacagtgcattgtaggttatctggttgaactacatgtgaactagcctaatattttcgaaataacattgaactaaccttcatagcctggtcaaaacacaaaacacaaaatactgggtgcgctaaataaacaaatgtaccgcagcgtcttaaatcgcatacatagttttataaaagagcaatataatcatcttttgtagcaaagtatgttttaaccatttaaatgctgcctataatcgtgtagcactgacaatgatttcgcttatgcgcactcttgactgaagccagggtaagcgccacaacacatcacacagaaatataaacaaatatataaatatacagctatatataaaacacaaacacacacaactatcgaataaaaaataagcaaaccagctgtttactgtatgacagatgttaaaatactgttttaatgttgacttcatatttgttatgtatttatatagattatattaaggaagaaaaaaaactttatccaatgaccggtgttgctttttatgtgagagtagatttctaaggtgtttaaattgcttatttatattgcaataacttctttatgtgaataaagcggtattggggcctttgcatgaaagctgcaaattgtcatattaggcatattcacgcgtgtttagaaaagtaaaaaagggtatgatcgagatttaagttgcagactgtatccatataccaaattgtggattttttttacttgtcgaatctttgttttacacctgaaaagagtgtgtgactatatagccaccaaacctaacacactttgccttacaagttcaagtgcgaacttaatatcacagaactgtgccgaatcagagaaacagtattagttcaattgcatatgagcatatctatgaatatggccttctatctgatatacgacctaatgaatttactcatcagcgaagatctatctataaaagctatgttgaaagctgaaatgaaaaaaattgagataatacaatgctatttttattttgtgtttacaggtactgtttgttatatgtgtaaataatttgtatcacgtacctacgtattaattgtgtaattaactgcctcctgaaatcttgataagaattaaataaacaatatgaataataa

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