LOCUS       NM_001039830            3252 bp    mRNA    linear   VRT 14-AUG-2025
DEFINITION  Danio rerio taste receptor, type 2, member 200, tandem duplicate 1
            (tas2r200.1), transcript variant 1, mRNA.
ACCESSION   NM_001039830 XM_073909825
VERSION     NM_001039830.2
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Behrens,M., Di Pizio,A., Redel,U., Meyerhof,W. and Korsching,S.I.
  TITLE     At the Root of T2R Gene Evolution: Recognition Profiles of
            Coelacanth and Zebrafish Bitter Receptors
  JOURNAL   Genome Biol Evol 13 (1) (2021)
   PUBMED   33355666
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Ohmoto,M., Okada,S., Nakamura,S., Abe,K. and Matsumoto,I.
  TITLE     Mutually exclusive expression of Galphaia and Galpha14 reveals
            diversification of taste receptor cells in zebrafish
  JOURNAL   J Comp Neurol 519 (8), 1616-1629 (2011)
   PUBMED   21452212
REFERENCE   3  (bases 1 to 3252)
  AUTHORS   Oike,H., Nagai,T., Furuyama,A., Okada,S., Aihara,Y., Ishimaru,Y.,
            Marui,T., Matsumoto,I., Misaka,T. and Abe,K.
  TITLE     Characterization of ligands for fish taste receptors
  JOURNAL   J Neurosci 27 (21), 5584-5592 (2007)
   PUBMED   17522303
REFERENCE   4  (bases 1 to 3252)
  AUTHORS   Go,Y.
  CONSRTM   SMBE Tri-National Young Investigators
  TITLE     Proceedings of the SMBE Tri-National Young Investigators' Workshop
            2005. Lineage-specific expansions and contractions of the bitter
            taste receptor gene repertoire in vertebrates
  JOURNAL   Mol Biol Evol 23 (5), 964-972 (2006)
   PUBMED   16484289
REFERENCE   5  (bases 1 to 3252)
  AUTHORS   Ishimaru,Y., Okada,S., Naito,H., Nagai,T., Yasuoka,A., Matsumoto,I.
            and Abe,K.
  TITLE     Two families of candidate taste receptors in fishes
  JOURNAL   Mech Dev 122 (12), 1310-1321 (2005)
   PUBMED   16274966
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JBMGRA010000008.1.
            
            On or before Aug 14, 2025 this sequence version replaced
            XM_073909825.1, NM_001039830.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR32588729.12959867.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMEA4476730,
                                           SAMEA4476732 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-121               JBMGRA010000008.1  32853605-32853725
            122-423             JBMGRA010000008.1  32853817-32854118
            424-558             JBMGRA010000008.1  32860190-32860324
            559-667             JBMGRA010000008.1  32860416-32860524
            668-694             JBMGRA010000008.1  32865455-32865481
            695-3252            JBMGRA010000008.1  32865859-32868416
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /strain="Tuebingen"
                     /db_xref="taxon:7955"
                     /chromosome="8"
                     /map="8"
     gene            1..3252
                     /gene="tas2r200.1"
                     /gene_synonym="T2R5; tas2r1a"
                     /note="taste receptor, type 2, member 200, tandem
                     duplicate 1"
                     /db_xref="GeneID:664690"
                     /db_xref="ZFIN:ZDB-GENE-070917-4"
     exon            1..121
                     /gene="tas2r200.1"
                     /gene_synonym="T2R5; tas2r1a"
                     /inference="alignment:Splign:2.1.0"
     exon            122..423
                     /gene="tas2r200.1"
                     /gene_synonym="T2R5; tas2r1a"
                     /inference="alignment:Splign:2.1.0"
     exon            424..558
                     /gene="tas2r200.1"
                     /gene_synonym="T2R5; tas2r1a"
                     /inference="alignment:Splign:2.1.0"
     exon            559..667
                     /gene="tas2r200.1"
                     /gene_synonym="T2R5; tas2r1a"
                     /inference="alignment:Splign:2.1.0"
     exon            668..694
                     /gene="tas2r200.1"
                     /gene_synonym="T2R5; tas2r1a"
                     /inference="alignment:Splign:2.1.0"
     exon            695..3252
                     /gene="tas2r200.1"
                     /gene_synonym="T2R5; tas2r1a"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    718..720
                     /gene="tas2r200.1"
                     /gene_synonym="T2R5; tas2r1a"
                     /note="upstream in-frame stop codon"
     misc_feature    724..726
                     /gene="tas2r200.1"
                     /gene_synonym="T2R5; tas2r1a"
                     /note="upstream_AUG_codon; putative N-terminal extension:
                     MQSNFAYEDHSPFLHKITCILPDVIQYQRRTLKRK"
     CDS             829..1791
                     /gene="tas2r200.1"
                     /gene_synonym="T2R5; tas2r1a"
                     /note="taste receptor, type 2, member 1a; bitter taste
                     receptor; zfT2R1a; taste receptor, type 2, member 200.1"
                     /codon_start=1
                     /product="taste receptor, type 2, member 200, tandem
                     duplicate 1"
                     /protein_id="NP_001034919.1"
                     /db_xref="GeneID:664690"
                     /db_xref="ZFIN:ZDB-GENE-070917-4"
                     /translation="

MSTDVGNVLFFVGVGVVGVSGNIFNLIFSLQQQVKTRSIQTVGLILDVISISNIILVLSTLAMVVSVFLNAHIWCIKPYPLGLRFEMYLMMTCGFISFWAIAWLSLFYCIKVVNFSSEIFRTLKKNISTVINTAVTLSCLFSFLLFLPAFSLDLPDSADKNISETNITTCPQPTFTLQIDINAYAAAVLLLICPIPLMIMLPTSVRMVVHLCAHTRALQKNQTQVQGSDSYLLVCKLTISLVGVYLSTLFMVALYFIIKVLGAFMTYQALVSAFTFYCGMTSVLLTASNRYLKDKLWSLFCCRKAKEPVSKSQTVVTQDV"

     misc_feature    928..1707
                     /gene="tas2r200.1"
                     /gene_synonym="T2R5; tas2r1a"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; Region: 7tm_GPCRs; cl28897"
                     /db_xref="CDD:475119"
     misc_feature    955..1020
                     /gene="tas2r200.1"
                     /gene_synonym="T2R5; tas2r1a"
                     /note="TM helix 2 [structural motif]; Region: TM helix 2"
                     /db_xref="CDD:320088"
     misc_feature    1078..1146
                     /gene="tas2r200.1"
                     /gene_synonym="T2R5; tas2r1a"
                     /note="TM helix 3 [structural motif]; Region: TM helix 3"
                     /db_xref="CDD:320088"
     misc_feature    1225..1275
                     /gene="tas2r200.1"
                     /gene_synonym="T2R5; tas2r1a"
                     /note="TM helix 4 [structural motif]; Region: TM helix 4"
                     /db_xref="CDD:320088"
     misc_feature    1360..1443
                     /gene="tas2r200.1"
                     /gene_synonym="T2R5; tas2r1a"
                     /note="TM helix 5 [structural motif]; Region: TM helix 5"
                     /db_xref="CDD:320088"
     misc_feature    1519..1575
                     /gene="tas2r200.1"
                     /gene_synonym="T2R5; tas2r1a"
                     /note="TM helix 6 [structural motif]; Region: TM helix 6"
                     /db_xref="CDD:320088"
     misc_feature    1609..1695
                     /gene="tas2r200.1"
                     /gene_synonym="T2R5; tas2r1a"
                     /note="TM helix 7 [structural motif]; Region: TM helix 7"
                     /db_xref="CDD:320088"
ORIGIN      

agagcagaaaataaatgagagagcagaaggtaaagcggacgtttcataacagtttaactagagctcgtactgaaaaggactgtttacaagtttaatcagcaaccccccgaaaaggcataagctcttacggtgatttcaagagtaaaggaatgtaaataaagcaacttgaaacatcagtaaagtttcggccatcattcggatggggtctgctacagtaagagcttcaccttcgctgattaagatcctcgcggcaggcgcaggcggacggatataacgcgacgacggaaagggcattgcagtgagttataatcagcatagagcgtgatggtctacggagctgagacgtaaaagggtacgagttcaatatggaaatggatgaagatgaccaagaatctgagaggccgctccaaatggaaaatgaaggaaatccgtttttcagagcagaaaataaatgagagagcagaaggtaaagcggacgtttcataacagtttaactagagctcgtactgaaaaggactgtttacaagtttaatcagcaaccccccgaaaaggcataagctcttacggtgatttcaagagtaaaggaatgtaaataaagcaacttgaaacatcagtaaagtttcggccatcattcggatggggtctgctacaacgtctccaacttctgccatacagatgacaatggagaagctttcctggaccaaagcctgctctcattaactcatgcaaagcaactttgcatatgaggatcattccccatttctacataagataacatgcatcttgccagatgtcatacagtatcagcgcaggactctaaagagaaagatgagcaccgacgttgggaacgttctttttttcgttggagttggggttgtcggtgtttctggaaacatattcaaccttatctttagcttacaacagcaagtaaaaaccagatccattcagactgtgggcttaatcctagatgtcatctccatcagcaacatcatcttggtactttccactctcgccatggtggttagtgtgtttctgaatgcccacatttggtgcatcaagccatatcctctcggtctccgttttgaaatgtatctaatgatgacttgcggcttcatcagcttctgggccattgcttggctgagtcttttctactgcatcaaagttgtgaatttctcctctgagatcttcagaacattgaagaagaacatctcaactgtgatcaacactgcggtgacgttgagctgcttgttctccttcttgctattccttccagcgttcagcctcgatcttccagattcagcggataaaaatatcagcgagacaaatatcacaacctgtccacagccgactttcactctacagatagacataaatgcatacgcagccgctgtcctgctcctcatctgcccgatccctctgatgatcatgttgcccacctctgtcagaatggtggtccacctctgcgcccacacacgggcactccagaagaaccagacgcaggtgcagggatccgactcgtatctcttggtgtgcaaactcaccatctctctggtgggagtttatctgtccactctatttatggtggcattgtatttcattataaaggttttgggggcatttatgacatatcaagccctagtcagtgcctttactttttactgtggaatgacttcagtgctactgacggcatcaaacaggtatctgaaagataaactttggagtctgttctgttgcaggaaagcaaaggagccagttagcaaaagtcagacagttgtgacacaggatgtctgatgtagtgcagtttagttaaagcgaaattcaacccccttagacctttcttcatcttcagaacactaattaatatatttaagagctctctgaccatctgtaaccccgccggtcctacgtcacccaacccgctcagagatggtgtccaaccggcgaccttctgcattggagttggttgctctaacaaggaggctaaagaccatggtctctagcgtctgtctctagagcacatttagaggtcagaggagtcaggtttacctgcacagcacttactagctggcctccgtttcacctacatagatagcaaaggtccagaaacattgtcaaaatctcatgtggtttaagtggttcaactataatcatactataaagctacaagaacaattctgtgtgccaaagaaaaactgtaaaaacatcttggtttgccaatgtgtcccgggaatacggcatacggaatgctgactctaatggcaatacattgtatatcctgcagtaaatgcctaaattgacaaccaagttcgaggagaaaaccttggtgaaaaaagttggttttactgtttttagggagctttagcttttaaatgattacagttgaaccactgaagtcagttttgacaatgtttttggctcattttctaaactttgaacatctcctggccattgctgcttatgagaataagggagctcttggattttcaattaaaatatcttaattgtgtcatttgtcattttaaacatgaacaaaggtctaaggggattggaacgacaagaaggtgagaaattaatagcggatattttattttaggtagaaataagctttaaggggacataaacagtagttcttcatgtttttatttacaatattcagactacaatactaaaaagcagatgctattttaatgtcactgtaaaaaaaagtaccataatgcaattcaaaagtgtaaattcacaggtgtatttactattttgaattgtattataagacctcaatctctgttctgtaaacatttaaagctgtaagtttaacaaagtgacttttattcacattttagtagcttaaagcaagacctgatatacatggcagtaaatctgtaaaataaaataaaagttctcctaatttgtatttcattcatatggttttccccgttttcatcagctacgcctcagtgatgtgtgatgtctatgtttaggatactgctactagctgtatgctactttttctcagaattcatacaaaatcgccacctgatttttacatgtgattggtttggaactggaaacttgtctatgtagaaaaacatcaaatttgttctcataatattgtgtaacgaatatgtctttgatttaatgaatggaatgctttaatttaattggtaatttaaatgtagagataaatgttacattagtatctagtttatgttattttaccgaactgtgataaatattaataaaatattccacatg

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