LOCUS       NM_001007782            1584 bp    mRNA    linear   VRT 05-OCT-2024
DEFINITION  Danio rerio NK2 homeobox 2b (nkx2.2b), mRNA.
ACCESSION   NM_001007782 XM_696524
VERSION     NM_001007782.2
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Chen,F., Kohler,M., Cucun,G., Takamiya,M., Kizil,C., Cosacak,M.I.
            and Rastegar,S.
  TITLE     sox1a:eGFP transgenic line and single-cell transcriptomics reveal
            the origin of zebrafish intraspinal serotonergic neurons
  JOURNAL   iScience 26 (8), 107342 (2023)
   PUBMED   37529101
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Bohm,U.L., Kimura,Y., Kawashima,T., Ahrens,M.B., Higashijima,S.I.,
            Engert,F. and Cohen,A.E.
  TITLE     Voltage imaging identifies spinal circuits that modulate locomotor
            adaptation in zebrafish
  JOURNAL   Neuron 110 (7), 1211-1222 (2022)
   PUBMED   35104451
REFERENCE   3  (bases 1 to 1584)
  AUTHORS   Mukaigasa,K., Sakuma,C. and Yaginuma,H.
  TITLE     The developmental hourglass model is applicable to the spinal cord
            based on single-cell transcriptomes and non-conserved
            cis-regulatory elements
  JOURNAL   Dev Growth Differ 63 (7), 372-391 (2021)
   PUBMED   34473348
REFERENCE   4  (bases 1 to 1584)
  AUTHORS   Truong,M.E., Bilekova,S., Choksi,S.P., Li,W., Bugaj,L.J., Xu,K. and
            Reiter,J.F.
  TITLE     Vertebrate cells differentially interpret ciliary and extraciliary
            cAMP
  JOURNAL   Cell 184 (11), 2911-2926 (2021)
   PUBMED   33932338
REFERENCE   5  (bases 1 to 1584)
  AUTHORS   Tschaikner,P.M., Regele,D., Rock,R., Salvenmoser,W., Meyer,D.,
            Bouvier,M., Geley,S., Stefan,E. and Aanstad,P.
  TITLE     Feedback control of the Gpr161-Galphas-PKA axis contributes to
            basal Hedgehog repression in zebrafish
  JOURNAL   Development 148 (4) (2021)
   PUBMED   33531430
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1584)
  AUTHORS   Schafer,M., Kinzel,D. and Winkler,C.
  TITLE     Discontinuous organization and specification of the lateral floor
            plate in zebrafish
  JOURNAL   Dev Biol 301 (1), 117-129 (2007)
   PUBMED   17045256
REFERENCE   7  (bases 1 to 1584)
  AUTHORS   Xu,J., Srinivas,B.P., Tay,S.Y., Mak,A., Yu,X., Lee,S.G., Yang,H.,
            Govindarajan,K.R., Leong,B., Bourque,G., Mathavan,S. and Roy,S.
  TITLE     Genomewide expression profiling in the zebrafish embryo identifies
            target genes regulated by Hedgehog signaling during vertebrate
            development
  JOURNAL   Genetics 174 (2), 735-752 (2006)
   PUBMED   16888327
REFERENCE   8  (bases 1 to 1584)
  AUTHORS   Schafer,M., Rembold,M., Wittbrodt,J., Schartl,M. and Winkler,C.
  TITLE     Medial floor plate formation in zebrafish consists of two phases
            and requires trunk-derived Midkine-a
  JOURNAL   Genes Dev 19 (8), 897-902 (2005)
   PUBMED   15833916
REFERENCE   9  (bases 1 to 1584)
  AUTHORS   Norton,W.H., Mangoli,M., Lele,Z., Pogoda,H.M., Diamond,B.,
            Mercurio,S., Russell,C., Teraoka,H., Stickney,H.L., Rauch,G.J.,
            Heisenberg,C.P., Houart,C., Schilling,T.F., Frohnhoefer,H.G.,
            Rastegar,S., Neumann,C.J., Gardiner,R.M., Strahle,U., Geisler,R.,
            Rees,M., Talbot,W.S. and Wilson,S.W.
  TITLE     Monorail/Foxa2 regulates floorplate differentiation and
            specification of oligodendrocytes, serotonergic raphe neurones and
            cranial motoneurones
  JOURNAL   Development 132 (4), 645-658 (2005)
   PUBMED   15677724
REFERENCE   10 (bases 1 to 1584)
  AUTHORS   Schafer,M., Kinzel,D., Neuner,C., Schartl,M., Volff,J.N. and
            Winkler,C.
  TITLE     Hedgehog and retinoid signalling confines nkx2.2b expression to the
            lateral floor plate of the zebrafish trunk
  JOURNAL   Mech Dev 122 (1), 43-56 (2005)
   PUBMED   15582776
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC091555.1.
            
            On or before Jul 6, 2005 this sequence version replaced
            XM_696524.1, NM_001007782.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC091555.1, AY729018.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA3505376, SAMEA3505378
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /strain="Singapore"
                     /db_xref="taxon:7955"
                     /chromosome="20"
                     /map="20"
     gene            1..1584
                     /gene="nkx2.2b"
                     /note="NK2 homeobox 2b"
                     /db_xref="GeneID:493627"
                     /db_xref="ZFIN:ZDB-GENE-050217-3"
     exon            1..439
                     /gene="nkx2.2b"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    196..198
                     /gene="nkx2.2b"
                     /note="upstream in-frame stop codon"
     CDS             244..957
                     /gene="nkx2.2b"
                     /note="homeobox transcription factor Nkx2.2b; NK2
                     transcription factor related 2b"
                     /codon_start=1
                     /product="NK2 homeobox 2b"
                     /protein_id="NP_001007783.2"
                     /db_xref="GeneID:493627"
                     /db_xref="ZFIN:ZDB-GENE-050217-3"
                     /translation="

MSLKNSKSGFTVRDILDLPDTNDEGSVSEDTEDEQDEGLNANQKKQHDSSRDALWLGSSCGHKYPVPSASPEELNPELSADESVDRESSEDSGKKRKRRILFSKTQTFELERRFRQQRYLSAPEREHLAKLLHLTPTQVKIWFQNHRYKVKRARAEKGLDPYLLSPRRVSIPVLVRDGRPCHLLGPQDITASIPPATAPFTAFNMKPFPQIHHSHNNIVTHLTSSVNHLGHMQPWSW"

     misc_feature    529..699
                     /gene="nkx2.2b"
                     /note="Region: Homeodomain; pfam00046"
                     /db_xref="CDD:459649"
     exon            440..1554
                     /gene="nkx2.2b"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

gcagaagcgctcgagcacttgactccacagatccacccttaatcgccaaggccgcttttttcctggagctccaacacttatccacgaccttaaatggaccagactaatggattggttcggtcaagcactgtaacatctgaaaagcatctgtgatccgtgtgtgcgctgtccaataggaaggatatttattatttataacttcaccgagagaccttcactgtcgcagtcctactggtttggaaaatgtccctaaagaattcaaagtcgggtttcactgtacgtgatattttagacctcccggacaccaacgacgaaggatctgtgagtgaagacactgaagatgagcaggatgaaggactcaatgcaaatcagaaaaaacagcacgactcctctagagacgcgctctggctcggatcttcgtgcggacacaaatatccagtgccgtcagcatctccagaggagttgaatccagaactgtccgcggatgagtccgttgaccgggaaagctcggaggactccgggaaaaagcgcaaacgaagaatcctgttttcaaagactcaaacctttgagttagagcgacgcttcaggcaacagagatacttgtccgcgcctgagcgagagcatctagccaagcttttacacttgactccaacgcaggtgaagatatggttccagaaccatcggtacaaagtgaaaagggcgcgggctgagaagggattggacccgtatctgctctccccgcgcagggtgtcaattcccgttctggtgagggacggaagaccgtgtcatcttctcggccctcaggacataactgcctcgattcctccggcaactgcgccgtttactgcctttaatatgaagccgtttccgcagattcaccacagtcataacaacatcgtgacacatctgacatcatctgtcaatcatctgggccacatgcagccatggtcctggtgaagagaacacgtacatcgcggagacgtcttgatttgatgtttgcgcttacatgtatttagagcatctgtgaaatgcctattagacgtcaatctgagacgttaaatatagacgtacgtgtttaaggtatttacaacttacaattacaagcagaacttttatgacattacaatgacgtcacaattgggagcagatctttttatgacgtttatcagacctcttaacacagcagatgttttccagaccaaatacttcttgaagacgaacgtgtgctatttgggcacggagcgcttttgttttcgtctttactgcaaggtggattctttatgtctatgttttcaattgtctaatattgtatttcccatctgttagttgcaatttaataaaaaaacaaaaaaaacatacacttgggacatcatgtagatatatttatgctggagagtatctttttgtattgatttcatttaagttcagttctctgatatgttccttttagttgcaattagaaaagactggggaaagtagattatagtatgttgttttttattttatttgaatagctggaattattcagactaacctgtaataaaaattgtgaataaaaaaaaaaaaaaaaaaaaaaaaaaaaaa

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