LOCUS       XM_067473715            2783 bp    mRNA    linear   VRT 20-AUG-2024
DEFINITION  PREDICTED: Anolis sagrei protein argonaute-1 (LOC132780766),
            transcript variant X3, mRNA.
ACCESSION   XM_067473715
VERSION     XM_067473715.1
DBLINK      BioProject: PRJNA1142516
KEYWORDS    RefSeq.
SOURCE      Anolis sagrei (Brown anole)
  ORGANISM  Anolis sagrei
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata;
            Toxicofera; Iguania; Dactyloidae; Anolis.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_090034) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_037176765.1-RS_2024_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/09/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Anolis sagrei"
                     /mol_type="mRNA"
                     /isolate="rAnoSag1"
                     /db_xref="taxon:38937"
                     /chromosome="X"
                     /sex="male"
                     /tissue_type="blood, liver"
                     /dev_stage="subadult"
                     /geo_loc_name="USA: Palm Coast, Florida"
                     /lat_lon="29.582251 N 81.214912 W"
                     /collection_date="2019-09-07"
                     /collected_by="Tonia Schwartz"
     gene            1..2783
                     /gene="LOC132780766"
                     /note="protein argonaute-1; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 12
                     Proteins"
                     /db_xref="GeneID:132780766"
     CDS             106..2454
                     /gene="LOC132780766"
                     /codon_start=1
                     /product="protein argonaute-1 isoform X3"
                     /protein_id="XP_067329816.1"
                     /db_xref="GeneID:132780766"
                     /translation="

MVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWMAIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA"

     misc_feature    148..372
                     /gene="LOC132780766"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    400..552
                     /gene="LOC132780766"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    553..915
                     /gene="LOC132780766"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(709..711,754..756,796..798,808..810,862..864,
                     883..885,889..891)
                     /gene="LOC132780766"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1048..2325
                     /gene="LOC132780766"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1459..1461,1471..1473,1507..1518,1525..1527,
                     1549..1551,1558..1560,1570..1572,1582..1584)
                     /gene="LOC132780766"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1663..1665,1669..1671,1879..1881,2293..2295)
                     /gene="LOC132780766"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

ctttgaggtggacatccccaagatcgatgtctaccactatgaagttgatatcaagcccgacaagtgcccgcgcagagtcaacaggtaagggaggttgtggaatacatggtccaacacttcaaacctcagatctttggagaccggaagccagtatatgatggcaagaagaacatctacaccgtcactgccctgccgattgggaatgagagggttgactttgaggtgacaattccaggtgaaggcaaagacaggatattcaaggtttctatcaagtggatggccatcgtgagttggagaatgcttcatgaggccttggtgagtggacagatccccgttcctctggaatctgtccaggcccttgacgttgccatgaggcacctagcatccatgaggtacaccccagtgggccgttctttcttctcgcccccggaaggatactatcatccgctgggaggaggtcgggaggtctggttcggtttccaccagtctgtcaggcctgctatgtggaagatgatgctcaacattgatgtgtcagcaacagccttttataaggcccagcctgtcatagagttcatgtgtgaagttctcgatatccggaacattgatgaacagccaaagcccctgactgactctcagagagttcgcttcaccaaggagatcaaaggtttgaaagtggaagtgacacactgtgggcagatgaagaggaaataccgtgtgtgtaacgttacccggcggccagctagccaccaaacgttccccctgcagttggagagcggtcagacggtggaatgcacagttgcacaatacttcaagcagaaatacaacctccagctgaagtacccccatttgccgtgtctgcaagttggtcaagaacagaaacacacatatctgcctctggaggtatgtaacattgtggctggccagcggtgcatcaaaaagttgacggacaatcagacttccaccatgatcaaagcaacagcaaggtccgctccagacagacaggaggaaatcagccggctgatgaagaatgccagttataacctggacccatacatacaggagtttggcattaaagtgaaggacgatatgacagaggtgacaggacgtgtcttgccagctccaatcttacaatatggaggccggaaccgtgccatcgccacaccaaaccagggggtctgggacatgagaggaaagcaattttacaacggcattgagatcaaagtctgggccatcgcctgcttcgcccctcagaagcagtgcagggaagaagtactgaaaaacttcacagaccaattgcgcaagatatccaaggatgcggggatgccaatccagggtcagccctgtttctgcaaatatgcacaaggcgcagatagtgtggagcccatgttccggcaccttaagaacacctactcaggactccagctcatcatcgtcatcctgccggggaaaacaccagtgtatgcggaggtgaagcgtgtgggagacacgctccttggtatggccactcagtgcgtgcaggtgaagaatgtcgtgaagacgtctccgcagaccctgtccaacctctgcctcaagatcaacgtcaagctgggagggatcaacaacatcttggtgccccaccaacgctctgctgtctttcaacagccagttatatttctgggagctgatgtgactcatccgccggcaggagatggaaagaagccctctataacagccgtggtgggaagcatggatgcccacccaagtcgttactgcgcgacggtgcgggtccaacgtccccggcaagaaatcatcgaggacctttcatacatggtcagggaactcctcatccagttctacaagtccacccgcttcaagcccacccgcatcatattctaccgcgacggagttccagaaggacagcttcctcagatccttcactatgagcttttggccatccgcgatgcctgcatcaaactggagaaggactaccagccaggcatcacctacattgttgtacagaagcgtcatcacacccgccttttctgtgctgacaagaatgaaaggattggcaaaagtgggaacatccctgcaggaacaactgtggacacaaacatcactcatccctttgagtttgatttctacctgtgcagtcacgctggcattcagggtacgagcagaccgtctcattactacgttctgtgggacgacaaccggtttacagcagacgagctccaaatcctcacctaccagctttgccacacgtacgtccgctgcacccgctctgtctctatcccagcaccagcatattatgcccgcttggtggcctttcgggcacgatatcaccttgtagacaaagagcatgacagcggagaaggcagtcacatatctggccaaagcaacggccgagaccctcaggcccttgccaaggccgtgcaggttcatcaggacactttacgtaccatgtactttgcttgaaagccgaaattttttacctcactccagaaagctttcagatttgtaggagccatacagaatattttaaaaaaagaaaataaaggaagcattgggagcactttggggcatcccatgagaaatcactgcagggcgccaacagcgttggaggcaggaaaccagcatcttccggggcaaatgcaagagagccgctcgctcgaccggatggacacaccttcttcagtatcgcaagagagactgtctttgttttttgggtgttccgattccctgaccaaacacagccgcggaagggatccaacttccgattccaaacggaacaggttttcgggggg

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