LOCUS       XM_067447816            8942 bp    mRNA    linear   VRT 19-AUG-2024
DEFINITION  PREDICTED: Pseudorasbora parva argonaute RISC catalytic component
            3a (ago3a), transcript variant X2, mRNA.
ACCESSION   XM_067447816
VERSION     XM_067447816.1
DBLINK      BioProject: PRJNA1148495
KEYWORDS    RefSeq.
SOURCE      Pseudorasbora parva (stone moroko)
  ORGANISM  Pseudorasbora parva
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Gobionidae; Sarcocheilichthyinae; Pseudorasbora.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_090178) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_024679245.1-RS_2024_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/16/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Pseudorasbora parva"
                     /mol_type="mRNA"
                     /isolate="DD20220531a"
                     /db_xref="taxon:51549"
                     /chromosome="7"
                     /tissue_type="liver"
     gene            1..8942
                     /gene="ago3a"
                     /note="argonaute RISC catalytic component 3a; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 7 Proteins"
                     /db_xref="GeneID:137082562"
     CDS             830..3439
                     /gene="ago3a"
                     /codon_start=1
                     /product="protein argonaute-3 isoform X2"
                     /protein_id="XP_067303917.1"
                     /db_xref="GeneID:137082562"
                     /translation="

MEIGTTGAVGAQSLFSVPRRPGYGTMGKPIKLLANCFQVEIPKMDVYLYEVDIKPEKCPRRVNREVVDSMVQHFKVTIFGDRRPVYDGKKSLYTANPLPVAPAGVDLDVTLPGEGGKDRPFKVSIKFVSLVSWHLLHEVLTGRSMSEPLELDKPISTNPVHAVDVVLRHLPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYEADPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRQINRELSLQNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEVIQDLASMVRELLIQFYKSTRYKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKEYQPGITYIVVQKRHHTRLFCADRNERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADEFQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTLRTMYFA"

     misc_feature    1046..1330
                     /gene="ago3a"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    1358..1510
                     /gene="ago3a"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    1511..1873
                     /gene="ago3a"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1667..1669,1712..1714,1754..1756,1766..1768,
                     1820..1822,1841..1843,1847..1849)
                     /gene="ago3a"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    2006..3310
                     /gene="ago3a"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(2444..2446,2456..2458,2492..2503,2510..2512,
                     2534..2536,2543..2545,2555..2557,2567..2569)
                     /gene="ago3a"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2648..2650,2654..2656,2864..2866,3278..3280)
                     /gene="ago3a"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

gcacaagctaatgataatgatggttatgatgatgacggtcactcgtggactcgcgcttcccctcgcagccaatcagcgcgtggctctcgcgccaggtccgtgcgtcgcgctcgcggaggatcagacgctccatatttagctgtagcgagtctcgcagaggctccgactgtcagagaaaccctgagagagagttcaactctcaacttatttcctgtcgccactggcttaaaacgaagggactaagcgctattgaaaagccactggcttcgagtgtggaattcagtgatgatcggggactttaagtgcagagaagactgcgtttgacgggatttttgccccctctcagatgggaatgtagcttagctggttacgttagcccggccactagcagtagcaggctagcgctgtcgaagagaggaggcgacataagtggactttcactcgctataacgtcataacacctcaacaaaccgcctcactctttccaaagacgcgtttgtgaaggcgtcgccgtcacagaaacagcgtttcgggactgtattggggcaggctgaggggaacttttccgtttagctgtcttagctagctggccgtcacgtcaatctgtcagccgaacctgaaatcccgaccagatagttcgagccagataccggggcaccgagcctcccaccgttgatatacgagcttctgggacggtggattaggagcccgcaacacgggaggagacgcttgctttgggttaacgggttagcggccgtcgggatccgagttgagtttctgaagttcgtctactgcgagccggggacggaaccggacagagacctcatgaatggaaatcggaactacaggagccgttggggcccagtccctgttctccgtgccacggaggcccggctatggcaccatggggaaacccatcaagctgctggccaactgcttccaggtggagatccccaagatggatgtctacctgtatgaggtcgacatcaagcccgaaaagtgtccacgccgagtgaacagggaggttgtggactcaatggtgcagcactttaaagtcaccatctttggggatcgaaggccagtttatgatgggaagaaaagtctctacaccgccaacccactgcctgtggcgcccgctggggtggatctggatgtcacgctgccaggggaagggggcaaagaccgaccctttaaagtcagtattaagtttgtgtccctggtgagctggcacctgttgcacgaggtactaactggtcgcagtatgtcagagcccttggagctggacaagccaatcagcaccaacccagttcacgctgtggatgtggttctacgccatctaccctccatgaagtacacaccagtgggccgctcattcttttctgctccggagggctacgaccatcctctgggtggaggcagggaagtgtggttcggcttccaccagtctgtccggccagccatgtggaagatgatgctcaacattgacgtgtcagccacggctttctacaaagcacagccagttattcagttcatgtgtgaggtactggacatccacaacatcgatgagcagcctcgccccctcacagactcccacagagtcaaattcaccaaagaaatcaaaggtctgaaggtggaggtgacacactgcggaaccatgcggaggaagtaccgggtctgcaatgtgacccgccgccctgccagccatcaaacgttccctttgcagttggagaatggccaaactgtagaacgaacagtagctcagtatttcagagaaaagtacaacctgcagctgaaatacccccacctgccctgtctacaggtggggcaggagcagaagcacacctaccttcccctggaggtgtgcaacattgtagcagggcagcggtgcatcaagaaactgacagataatcaaacatccaccatgatcaaagccacagcacgatcagcaccagacagacaggaggagatcagcagactggtgcgcagtgctaactatgaagcagaccctttcgtacaggagtttcagtttaaggtgcgagacgagatggcccacgtgacggggcgcgtgttgcctgcgcccatgctgcagtacgggggcaggcagatcaaccgtgagctgtcgttacagaatcgcactgtggccacacccagtcatggagtttgggacatgagaggaaaacagttccacaccggggtggagatcaagatgtgggccatcgcctgcttcgccacacagagacagtgcagagaagagattcttaagggtttcacagaccagctgcgtaagatctcaaaggatgctgggatgcccatccagggtcagccgtgtttctgtaagtacgctcagggagccgacagcgtggagcccatgttcagacacctgaagaacacctactctggactgcagctcatcatcgtcatcctgccgggaaaaacacccgtctacgcggaagtgaagcgtgttggagacactcttctaggaatggccactcagtgtgtgcaggtgaagaacgtggtgaaaacctcgcctcagacgctctccaacctctgcctcaaaatcaacgtcaaactcggcggcatcaacaacatcctggtcccgcatcaacggccttcagtgttccagcagccagtcatctttctgggggcggacgtcacacatccacctgctggagatgggaagaaaccatccattgcagcagttgtgggcagtatggacgctcatcccagcaggtattgtgccacagtgcgggtccagaggcccagacaggaagtgattcaggacttggcctctatggtgcgagagctgctcattcagttctacaagtccactcgctacaaacccaccagaatcatcttctacagggacggtgtatctgagggacagtttagacaggtgttgtactacgagctgctggccattcgagaggcctgtatcagtctggagaaggagtaccagcccggcatcacatacatagtggtgcagaaacggcaccacacgcgcctcttctgtgccgatcgcaatgagagggtgggtcgcagcggaaacattcctgctggtacgacagtagacacagatatcacgcacccctacgagtttgacttttacctctgcagtcatgctggaatacagggcactagccggccctcccactaccatgtcctgtgggacgacaactgcttcactgctgacgagttccagctgctcacctaccagttgtgccacacatacgtgcgctgtacccgctccgtctccatccctgcgccagcctactacgcccacctggtggccttccgtgcccgctaccacttggtggacaaagaacacgacagtgccgagggcagccacgtctctgggcagagcaacggccgggatccccaagcgctggctaaagctgtgcagattcaccacgacaccctgaggaccatgtactttgcctaagccagctcacatcaagcccacgttcgactagtcatgcacacgaaaccaacaaccacatacacatgcagtcacacctcccaaaagcctgtgcgtcccgaagggtcgatctctgtggcgatggaagcacggctcagcccgacactgtgcagcaggatgaggaaggcctgctttaattccacacgtggacctcggcgctattatgcaatattaaaccagccaactgaggccgttctgtcatatcccctccaaacctttgacaacaaccaaaagggaagcccgtctatatccctgctcctcttcctgtctgtagacgtaatgtttaattacttcccctgcccgtcacgccttgtacctcaacgttttctaacagcacttttcccccctattatcctcccccttcccccaaaatgagattcgaaaggaagcagatggaaagactgagttgggaagaaaggaagtttcggggatagaggcatcacacgctcttactagcaattttctcaatgtacttttctcccgttctcaattatttttgtagccaatggtgacttaactacgtgttaaagtgtcagtcgtgaagaaccaattgtgcgttagagttttaaaacagaaaaacaagcttttattgttagaaaagtgccattgccgagcgtaaagtcctttatgacgttccccgccagcggggcagaaagctgaccgttgatttttaaagctaatgttcccattttacgctcagtaaaatctacctggcctgtgagctttcatgtactacaatgcaaagtgatcatgttttgatatgctttttcttttttctttttttaacacccaaattccctccaatatttatgtcttgatcaacgcagctcctagcagatttgtcttgttcacttcccttctgcactttgtctcaatttaaaatgcatagtccgtgttgtgacagatcatagtttagtgcaataatccattcgcgggatgagtacctcctgtgcaaagactcacagttcgctggaagtccgtagtggtgaaaaaggcactcttgctgttggtttttaaggtggaggtgtggcacttttatagttttctgaaagtagaggtgatgattgaccatagtgtgttaacaagtgcctgcttgatgttggggaggtcgaacagaattggatagatgctgtgggaaataacgaacgcaactcatctagtccaaacaagggcacagttgcgaacacattcgactgtacggccttaacaaaatctcttaacgttaacgtcttggttttcactgccctgcctgttcaaaggacttcgctgctattgtacttgttaaagtcagactggcgggttcatgcttcggtttcgcccaagcacgagggaccgattggcagctctgggccgtgtgtttaaggagaagctctcacagcacctgatgtgtgctctttcactggagggccactaggggtcagtatagtattttacttgaagagtgcatctcggtgcagtgagcgttgggtgccttactgtctctgtgcgccatctgaagcatgtccagctgtttaaaggtgataatctgcactaaacaatgcgtaatgcatggtgctattaggtgcggcccatggcggcatcagcgatttggcggagacttaatgtgtaacgatgggaacatggtatgcataaatggtgctggttgcatatatttctctcactccgagggggagattctcatcaggacagacatttggtcgttttgtgtgtttgttgtttgttttgttgatttgcatttgcatttgattttattgcgtagaaaggtacagtactgtggccagtcagaatacgattacggtcaattgcacaaagaaagtggaagattttaatgcatagcctaattatgacttttcaatttcagcccttttcaaaatccagatctctttatgcaatcagatgtttgctttttaaattctaaagtatgaataatcgcctgttaagaatgaaactaacgcagtgggatgttatatagagggttttctgcagggtttttaaaaaaagtgtgcattagaggtcggacctgtagaggaaggtacatgttttctgcccatcaggcctatcaaaagtggtttaagtgaggctgtaagcatcaaaacagcagctactgaaattgagcccttttttatttcaccttccctaataaaaccactattggtgcttcagagccttgaaagtaaaaccgcctgtgtgcggatgccaaacagctatcagtttaagaagcaatcttgatgaggtgtcatgctattttatttttgtgtagttttaacttcatatatatatatattactaacccttgcctcttgtttaggcaagaaatggatagttcggttataagtgattgtcattatttactcgcccacatgacttttctatcgctgaacgtggtgtacgtggtgttttaaagagcgtttgagttgctgtccatgcaacaaaaggcaaggatatcagtaaatgattgtcattttggagcttgctacactgtaaaaaaaaagtgtgctgccttaaattttaaagttgagtcaacttaaaaagtgaagttgcactaagtgacaacttggatatttgagttgacaacttaatattttaagtaagcacaaacttttcagtctatttttgttcagtccaagtcaacagctttagacaacgttctgtgaaattgcttcttatgaaagaagtgaatgtttgaatgacatgagggtgagaaaatgcttatgtgattgtctaatttttatctgaacgattcttcaaagcgacgaacggttttacccgaagatcgtacagagtgttagtgatgtatcggctgtggtagcagatgttgttgcaggggactcggttggttcgtagcagagtttcagtgcttgagtttgttgcctgacgtggcttccgttatccgtaaacctagaagtcaaacagccacatcccagtcagacaacgtattttctcctctcctctctcgtcttttaatttccagctttttgggagggtcttcttttagggtctaaaacgttacacgagttcagagttcatagaaagtgcctttcctcatgatggtgctgaataatctgagactaaagtgagaaatcaaagagcatattagtcttattcttaccttgaagaaggtgttgcacacatgcaaacacgcacgaacacattttatctcgatcgggaggctcggtgcctctttgtatattcagatatattatagtgacgatgatatatgccaagtatacgagcgaacggttctgtatcatactgtattaggagtccggaccaatgcaatggaagcgttcttataggtgaatgtgctatctttagatgggggcagagcggacactcatatgacacggcctgaccctggacaggaaagcctagcacagcactttaaagactgccacctaaacaaggaaacaaagtcacagtgctttgctctccttttccttttccccccataaacccttttttcatgtactccagcaaaagtctctagctcttgaagtctttgctgagggaaacttgagcgagattaattgaggtttacacaaggctccggtgacgtctttttggacttttttttattgccgtgactgatcgatccctctcaatgtgcaatggcccgtatagatctacatacagtatatgttttaaacatgagattgacccccatggcctgtgttttgtcacatgggtgcccgttggtccaggaggatatacctcgatgtagatctcaaaacactgcacctcacttaccttggtatgctgcaaatgttatatagaatgggtggatctcacgaaaatacacatcctgctcacgtttcaaattatgttttgcatgacaaaaaggaaggctattttcgtgcattgcggcaattaaacaaatctccccctccccaaattctgatgcatttgtacaccaccgtaatattgcttttcatttacgcagatttaaatgtaataatgcacatcgttctgttactattgaggtcgatgggaattacaaaactactcaactgggagaaattatgaaaataatgcaacaattgtatttgttgctataatatgcttaatttttgtccgttatgcttaattatattttccattaggaaaattaagccttttttggcattgtgaatatttttgagactattaaatgcatctccaaattctcattatgcaaacaaaaatattaaactgtattcatacatcatggtagtatttgtgcattgccttccatttatgctgatttaatgtaatacagcaaatatcattttatcaccattgatgggaactacaaaaccgctcaaaacgttgagagaaaagtgcttaattctcgtgaaaataatacaacagatgtattcgttgttataatattgccttattttcatgcaaaatgtcatttctagtgcacgacacacaaataagcaattgtattttgcataaagaaaatgaaattactatttctatgatgattgaatgcattcccccctctgcaaattctcatttatgcaaaccaaaatatatatatataatattaaaatgtattcatatatcatgtagtatttgtcgcattggctttcatgtatgctgatttaatgtaaggcagcaaaaatgaattacaaaacgccgtttgggagaaattcgcttaatcgttgtggaaataatgcaagaaatatcggcatcgttttccctgtgcaaaatatacttttcctttgattttggggtggatatgttttcttgagattcatccagaaattacgttgattacagtatattgcttgactaatgcctgacaacaatatccttaacgattttagagcaagaagtctaattgttagaaaacaagttgtttttaaatcataacacgtatctttttcttcttttattgagccaacttttttttattttgttgtcctatttaaatgtgttttgtttttttcttttaatatattggaaatatatatgggaaatatttaacggtgtatttattgagggagtgctttcgcggctggagtctccgcatcgttggccagagtcacgggggggcgagttgggggtcggggggtcagacgccctcgacgtcatcctctcacaagcacaaccacagagtgggcggggccggcacaagcctgaagtgttggatgacatcacttcctccttgcccttttgactagatcgtccacctcattcttcagctctccagcgaagaggctcgtcgcatctcatctctttacgggacggagaaccacactgactttattccaaaaagataaaaagctgattgttttcatccagagctgctaaactcttagccatacacgtttccctggaaggatgtttgatgcgtccggtgctttaagtggagtcgagaagcagatactgctatttgtccttcatcatttgcttgagttcaattgtcttcgtgccagagagggggtcccattccactcagaaatatataaatacattttta

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