LOCUS       XM_056122743            6788 bp    mRNA    linear   MAM 09-OCT-2023
DEFINITION  PREDICTED: Sorex fumeus argonaute RISC component 4 (AGO4),
            transcript variant X2, mRNA.
ACCESSION   XM_056122743
VERSION     XM_056122743.1
DBLINK      BioProject: PRJNA972655
KEYWORDS    RefSeq.
SOURCE      Sorex fumeus (smoky shrew)
  ORGANISM  Sorex fumeus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Laurasiatheria; Eulipotyphla; Soricidae;
            Soricinae; Sorex.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_026606104) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_029834395.1-RS_2023_10
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.2
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 10/04/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Sorex fumeus"
                     /mol_type="mRNA"
                     /db_xref="taxon:62283"
                     /chromosome="Unknown"
     gene            1..6788
                     /gene="AGO4"
                     /note="argonaute RISC component 4; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 5
                     Proteins"
                     /db_xref="GeneID:130031409"
     CDS             589..2973
                     /gene="AGO4"
                     /codon_start=1
                     /product="protein argonaute-4 isoform X2"
                     /protein_id="XP_055978718.1"
                     /db_xref="GeneID:130031409"
                     /translation="

MVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDMEVTLPGEGKDQTFKVSVQWVSVVSLQLLLEALAGHLNEVPDDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEISQELLYSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTQHTMYFA"

     misc_feature    598..852
                     /gene="AGO4"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:435368"
     misc_feature    883..1035
                     /gene="AGO4"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:430160"
     misc_feature    1036..1398
                     /gene="AGO4"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1192..1194,1237..1239,1279..1281,1291..1293,
                     1345..1347,1366..1368,1372..1374)
                     /gene="AGO4"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1537..2844
                     /gene="AGO4"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1948..1950,1960..1962,1996..2007,2014..2016,
                     2038..2040,2047..2049,2059..2061,2071..2073)
                     /gene="AGO4"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2152..2154,2158..2160,2398..2400,2812..2814)
                     /gene="AGO4"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

ggctcccgctcccgctcccgctggctgcggcggcggcggcggcggcggggattgtttttgttgtcgctgaggccggaagagccgcaggagccgggtccctgtccccgggccgggcgccgccgccgccccctacccagcggccgcgtctccgcggcgcccccccagcgccaatattccggagatcaagcgttacgcggcggcggcggcggcggcggcggcggcggcggcggggcccggagcgggaggcgccggggaccggggcgaggcggccccggccgccgccatggaggcgctgggacccgatttaatttgctgatgttgttaccaaatgtttagatgcctccattataaaatcctggaacctttgagcaatgactataacaattatgcaaatacagtcataaaggacctccgaccagcctgtttcagccacctcgtcgtcctggccttggaactgttggaaaaccaatcagactgttagccaatcattttcaggttcagattcctaaaatagatgtgtatcactatgatgtggatattaaaccagaaaagcggcctcgtagagtcaacagggaggtagtagatacaatggtgcgacacttcaagatgcaaatatttggtgaccggcagcctggctatgatggcaaaagaaacatgtacacagcacatccactgccaattggacgagatagggttgatatggaagtgacccttccaggtgagggtaaggaccagacctttaaagtgtctgttcagtgggtgtcagttgtaagccttcagttgctgttagaagccttggccggacacttgaatgaggtgccagatgattcggtacaagcccttgatgtcatcacaagacaccttccctccatgaggtatactccagtgggccgttcctttttctcacctcctgaaggttactaccaccctctgggagggggcagggaagtttggtttggttttcatcagtctgtgagacctgccatgtggaatatgatgcttaacattgatgtatctgcaactgctttctaccgggctcaacctatcattgaattcatgtgtgaagttttagatattcagaatatcaatgagcagaccaaacctctaaccgactcccagcgtgtcaagtttaccaaagaaatcagaggtcttaaagttgaggtgactcactgtggacagatgaaacgcaaatatcgagtttgtaatgtcactagacggccagccagtcatcaaacttttcccttgcagctagaaaatggtcaagctatggaatgtacagtagctcaatactttaagcaaaagtatagtttgcagctgaaatatccccatcttccctgtctccaagtgggacaagaacaaaagcatacatacttgccacttgaggtctgtaatatagtggcaggacagcgatgtattaagaagcttacagacaatcagacttccacgatgatcaaagccacagcaaggtctgctcctgacagacaggaagaaatcagtagactggtgaagagtaacagcatggtgggtggacctgatccataccttaaagaatttggtatcgttgtgcacaatgaaatgacagagctcacaggcagggtacttcctgctccaatgctgcaatatggaggccggaataaaacagtagccactcccaaccagggtgtctgggacatgcgaggaaagcagttttatgctggcattgaaattaaagtctgggcagttgcttgttttgcacctcagaaacaatgtagagaagatttacttaagagttttactgaccagcttcgtaaaatctctaaggatgcaggcatgcctatccaaggtcaaccatgtttctgcaagtatgcacaaggtgcagacagcgtggagcccatgtttaaacatctgaaaatgacatacgtgggcctacagttaatcgtggttatcttgcctggaaagacaccggtatatgcggaggtgaaacgagttggagataccctcctgggaatggccacacagtgtgtccaggtgaaaaatgttgtgaagacctcaccccaaaccctttccaacctttgcctgaagataaatgcaaagcttggaggaatcaacaatgtgcttgtacctcatcaaaggccctcagtgttccagcagcctgtcatctttctgggcgcggatgtcacacaccctccagcaggcgatgggaagaaaccctccattgctgctgtggttggcagtatggatggccaccccagccggtactgtgccactgttcgagtgcagacctcccgccaagagatttcccaggagcttctctatagccaggaggtaatccaggaccttactaacatggttcgagagttgctgatccagttctataaatctacacgtttcaaacccacgcggatcatctattaccgtggaggagtatcggagggacaaatgaaacaggtagcttggccagaactaatagcaatccgaaaggcatgcattagcttggaagaagattaccggccaggaataacctatattgtggtacagaaaagacatcacacacgactcttctgtgcagacaaaacagaaagggtggggaaaagtggcaacgtcccagcaggcactactgtggatagcaccatcacacatccatctgagtttgacttttacctctgtagtcatgcaggaattcagggaaccagccgtccttcgcattaccaggtcctgtgggatgataactgcttcactgcagatgagctccagctattaacttaccaactgtgccacacctatgtgcggtgcacacgctcagtctctattccagcccctgcatattatgcccgacttgtagcattcagagcaagatatcatctagtggataaagatcatgacagtgctgaaggcagtcatgtgtcaggacagagcaatggccgagatcctcaggccttggcgaaggctgtgcagatccaccatgatacccagcacacaatgtattttgcctgagagtgtcagaaaaagaactcaaccagcttggcaccccattcagcctcataatgtttcaaatgcctaccgcctctagagagccaagttgacttcaggtggtcttctaccagcagcttggaatagttgcactgattctattcttcatagcactgtctaatgcatcaacaaaatcgagccatttaaaaaaaaagaaaaagtaatagatactcatagattgtcttttctgatgcactggactgaatttttacctcatcatacaaaggctgatcagcctgaactgtctaaaggactttacaagaaaggtttctatggaatattttgctagctgtggtgttcaccgcatccctctagtcttataaaagaagattattccttttttctgtattcattttgtggggtatatgcgtaagtgggagagaaaaaccaaacaatttacttcagttcagcgtaactatttaattgtgtgggtacacagactttttaatggagatttctgactctgccactgtaaatgcctttaacgagcattaatttttttaaatttttacagagttttattagttttactactttatcttataactgactacagacttgtgctagtgcaagtcaggctgccaggcaatcacactgtatttggctgtagattcatatgggcagttctgttatttggttgacttctgtgaatgtgtaacacaagcagatgtaatgcatatcatggtggccggtaacactgccatggcaaaaagcactcatacttcccccactttgagaaaaaaatattagcttttagtatttttcaaagttttcaaaagtgcccattttatgctgctgtgtggtttgttagatctaaatgacttttaaacttttctcatagaaaattaacgttggcaataaacattttttaaggtcccatctcttcctccccaacaatgtagttttctagatgagattttggtatgattttatcagctctttcttattgatcataatctggcaatttctgatactggtcagataagacatatatttccatgctttttaaaagaattatttttatctgttttatagtcacaattggtgtcctgtcattttgttttaaaataagctagaaccaggatgcaattggatgggctttgccgatccttaaggtacattgagtgatgctgcaacttgcaaaaatgtaccagcatttaaaacttctttgcctggggatcaaggttacccacagaattacttgcagtgtacattttttcttccccttctctatgaaatgtacttagtggggtttccttccttttataaaattaaaactgcaagaaaagattaatttctctttaaaagattctgaatcctcttcataaggagacaggattcctttcttatccaaacaaaggaggttgatctgaagcaagcatcagctgaaatgtaaggggaaatgtattttgtatttagtgtagataatactttgtgaatggacagcagttacatagaatcttggtagttccagccagtgtttcccagccccagttttactgacttgtggttcactagcctagatcctcaggagtgagagctaagaaaggggcagaaaaatcccagagaagccacactccataggactgaggaaggtgctggggaagacgcagacgcagagcggtaaatgactttgccgcctctgtctggaaaccactggtaggggaacactggatctggtggtgaagggtgcttgcatatactcattagccagtccaaatctcttcaactagccttctcaaaatcccagcaatataaaacattgtttttcttcagtcttttaaataagcttgtaaatacattgaagtttacatttccacgtgctgttgatcagcggcgccaccagtgtttgctgaaactggtctcttgagggttttataatgtatatcgagtcctatagctcagtaagatggctgcagatatcattattctctgcgtatagggggtgaaagcaagctctgtgattgaggcacaaaataggtaggagtgaagaaatggcaatgttaatttttaagattttccttttttttttttttggcaaatacctcacttggataatacaaatcaggacatgttggtgaggggctggctgctgcttttattcacacttgttcagggagagcacagaaaatgcccttcagcatggccaccctgaacccaagaggaggacacagatccttttttcttcttcttcttctttttttttcttttttaggagcaggaggactctcaggggtccaggatatgaagaattcttggtggctggctggctggctggctggctggccagcaagccccacacttgtacttgagtgcctctggtttatgaataaggcacagagcacaaaggagcatcgcttcttaccctctccttgcctgtatggagcagagagtgaggcagagtaccagaaagccctgatcacttgcctgcagttgactatatgctcgaccttgaactatgtacagttcgtatgacttagtcagtcctagtacactggagagaatcagtgtggtggtggaggaaatgccgggaggcctattttttttttttaaatgcaggaaaacagcacctttaagggtgcaatgctgtcgtttagagtgaaaagaaagaaatccagaagaacaaggattattagaaaaaaacttgctgagggttgaattttaagaggtaattgctgcagaatccgagttacagcaatacacttgcaagcaagactgaatgggcagttttcatgtaattgggcctgactcgggactgactgaattgttatgcatgtgtcaatcaatgggaggtgtgcatctctagaagtagtttttggctgtttgatttttttaaatgcagcctgtttaggcactgttatgtggtgtgaaagatttaaatgtagcaatgcgaaagagtaacaatttgggaataagagttgggggcttatgaagagagtggcaaagacccaaactttgaaccaattatgttttactgttgttagctgttgacttctgaatggtttgtaatggcttcatgcagccagcagtctgggcaggcagaacacgttaattcttactgtaaattctatttgctgcttccaaaggtgatgatttacaagcagacatattccatatgatctgtaccttaggatctggtgcccagcctatttaccagagcttatctacctgcttcagctacctgccctcatgggaaaggtaattaattcactgtttcacaaggctcactctctctttccagccctcgtgaccctttgtggatgtgctgtctgactcttgctcaatagcaaagtggtagctctgctttcattttaagaaagtggaggttgagggcattgtatcaatactgcttcagttccaaggatttttcctcataaaattaaagggaagatcttactgatcaaccatttccttgtcccttgctattgacatattcatgctctttctgtgtctagtggctgaaaatgtttgcatttgttcacttgactaatggtgtaatttttgtttctatattgttagacttgtaatgttttaaaaatgtattttattaaatttgaactggatgtatgtctctagcaatacgaggtactttctaaactattaagggaggggtggtattcccatgttgagataaaaggatggttgtttaaattttacaaattttttggcctgcagggatattttgtgttcatgtgtcaaaaaaaaaaagaaaaaaagaataaattggcatttgtgtgccaaaagttgtttttcttgtcaattgtctaataaatatggag

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