LOCUS       XM_050155666            5023 bp    mRNA    linear   ROD 12-SEP-2024
DEFINITION  PREDICTED: Alexandromys fortis protein argonaute-4 (LOC126509628),
            transcript variant X2, mRNA.
ACCESSION   XM_050155666
VERSION     XM_050155666.1
DBLINK      BioProject: PRJNA868355
KEYWORDS    RefSeq.
SOURCE      Alexandromys fortis (reed vole)
  ORGANISM  Alexandromys fortis
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Cricetidae; Arvicolinae; Alexandromys.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_026067894) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_014885135.2-RS_2024_09
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 09/12/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Alexandromys fortis"
                     /mol_type="mRNA"
                     /isolate="MF-2015"
                     /db_xref="taxon:100897"
                     /chromosome="Unknown"
                     /sex="female"
                     /tissue_type="muscle, liver, lung and blood"
                     /note="4th-generation inbreeding"
     gene            1..5023
                     /gene="LOC126509628"
                     /note="protein argonaute-4; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 1
                     Protein"
                     /db_xref="GeneID:126509628"
     CDS             386..2722
                     /gene="LOC126509628"
                     /codon_start=1
                     /product="protein argonaute-4 isoform X2"
                     /protein_id="XP_050011623.1"
                     /db_xref="GeneID:126509628"
                     /translation="

MEALGPGPPASLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDVDIKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDMEVTLPGEGKDQTFKVSLQWVSVVSLQLLLEALAGHLNEVPDDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEIAQELLYSQEVVQDLTSMARELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTVTHPSEFDFYLCSHAGIQCGRQSRVRTEQWPGPSGFG"

     misc_feature    461..850
                     /gene="LOC126509628"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    881..1033
                     /gene="LOC126509628"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    1034..1396
                     /gene="LOC126509628"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1190..1192,1235..1237,1277..1279,1289..1291,
                     1343..1345,1364..1366,1370..1372)
                     /gene="LOC126509628"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1535..2662
                     /gene="LOC126509628"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1946..1948,1958..1960,1994..2005,2012..2014,
                     2036..2038,2045..2047,2057..2059,2069..2071)
                     /gene="LOC126509628"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     polyA_site      5023
                     /gene="LOC126509628"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

gccccctgccccaggccggctcgctggaccccacgcgctgcggggccgcgcacgcccccttcgccggccgggaccccgggtgcgcgcgctcggcgcttccccctccccctccccgcccggcgggcgcgcgccgcctcccgctcccgctccagctggcggcggcggcggggattgtttttgttgtcgctgaggccggaagagccttgagagagccgggtcccggttccccgggccgggcgccgccgccgccccctgcccagcgcccgcgtcttcgcggcgccgcctcagcgccaatattccggagagcaagcgttacacggcggcggcggcggggcccggagcgggaggcgccggggaccggagcgaggcggcccccgtcgccgccatggaggcgctgggacccggacctccagccagccttttccagccaccgcggcgtcctggccttggaactgttgggaagccaattcgactgttagccaatcattttcaggttcagatccctaaaatagatgtgtatcactatgatgtggatattaaaccagaaaaacggccccgtagagtcaacagggaggtggtagacactatggtacgacacttcaagatgcagatatttggggatcggcagcctggttatgatggcaaaagaaacatgtacacagcacatccactgccaattggacgtgatagggttgacatggaggttaccctcccaggtgagggtaaagaccaaactttcaaagtatctttgcaatgggtgtcagttgtgagccttcagttgctcttagaagctttagctgggcacctgaatgaagtcccggatgactcagtacaagcacttgacgttattacaaggcatcttccctctatgaggtacactccggtgggccgttcttttttctcaccccccgaaggttattaccaccctctgggaggtggcagagaggtctggtttggctttcaccagtctgtgagacctgccatgtggaatatgatgctcaacattgatgtatctgcaactgctttttaccgggctcagcccatcattgagttcatgtgtgaggttttagatattcagaacatcaatgaacagacaaaacctctaacagactctcagcgtgtcaagtttaccaaagaaattagaggtctcaaagttgaggtgactcactgtggacagatgaaacgaaaataccgagtttgcaatgtgacaagacggccagccagtcatcaaacctttcctttgcagctagaaaatggtcaagctatggaatgtacagtagctcagtattttaagcagaagtatagtctgcaactgaaatacccccatcttccatgccttcaagtgggacaagagcaaaagcatacatacttgccacttgaggtctgtaatatagtggcaggacagagatgtataaagaagcttacagacaatcagacatccacaatgatcaaagccacagcgagatctgctcctgacagacaggaagaaatcagtagactggtgaagagcaatagcatggtgggtggacctgacccatacctgaaggaatttgggattgttgtccacaatgagatgacggagctcacaggcagggtgcttccagctccaatgctgcagtatggaggccggaataaaacagtagccacacccaaccagggtgtctgggacatgcgaggaaagcagttttatgctggcattgaaattaaagtttgggcagtggcttgttttgcgcctcagaaacaatgtagggaagatttactaaagagtttcaccgaccagcttcgtaaaatctccaaggatgcagggatgcccatccagggtcagccatgtttctgcaagtatgcacaaggtgcagacagtgtggagcccatgtttaaacacctgaaaatgacatatgtgggcctgcagctaatagtggttatcttgcctggaaagacaccagtatatgcggaggtgaaacgagttggagatacccttttaggtatggccacacaatgtgtccaggtcaaaaatgttgtcaagacctcgccccaaaccctttccaacctttgcctgaagataaatgcaaagcttggaggaattaacaatgtacttgtacctcatcaaaggccttcagtgttccagcagcctgtcatcttcctgggagcagatgtcactcatccacctgctggggatgggaagaagccttctattgcagctgtggtgggcagcatggacggccaccctagccgatactgtgccacagtgcgggtgcagacgtctcggcaggagattgcccaggagctcctctacagtcaggaggttgtgcaggacctgacgagcatggctcgggagctgctgattcaattctacaagtccacacgtttcaagcccactcgcatcatctactaccgtggaggggtgtcggagggacagatgaaacaggtagcttggccggagctaatagcaattcgaaaggcatgtataagcttggaggaagactatcgaccaggaataacctacattgtggtgcaaaagagacatcacacacgcctcttctgtgcagataaaacagaaagggtggggaaaagtggcaatgtcccagcaggcactacagtggatagtaccgtcacacatccatcggagtttgacttttacctctgtagtcatgcaggaattcagtgcggaaggcagtcacgtgtcaggacagagcaatggccgggaccctcaggctttggctaaggctgtgcagatccaccacgatacccagcacactatgtattttgcctgagagtcttggaaaagaactcaaccaatttggcatcccacgcatcctcaaatgtttcagatgcctactgcctctagatagtgccacgttgatttcaggtggtcccctaccagcagctcggaacagttagttgcactgaatctgtactttgcagccctgtctgatgcatcaacaaaattgagccattttttaaagaaaaaaaaaaagaaatagaaactcatagattatgctttctgatgcactggacaaaaattttacctcagcgtgcaaaggctgatcagccttgactttctaaaggacttgttttcaaaggagggtctctgtgggatattttgctagctgtggtattcaccgcatctagtcttataagaggagtatttcctttttccttgttcattgagtggggtgtatgcataagtgggagagaaaaaccaaacgacctgtttcatttccatgcaactttttaattgggtgggcccatagactttttaatggagatttctgacttcgccactgtaaatgcctttaaggagcattaatttttgaaagttttacagaattttattactttcactgctttatcttactgatctctttgggcctgtgctgtggagtgcaggctgcctggcaattgcactatatttggctgcagattcaagcaggcagttctcttacgtgtctggctttgtgaatgtgtgacataatcaagtgtgatgcgtgtcacagggacagatagcactgccgtggtcaaaagtaaaaagttctccctatttggaaaaaaattagcttttaatacgtttctaagttttttaaagtgcccattttatgctgctatgtggtttgttagatctaaatgatttttaaacttctctcatagaaagttaactttggcaataagcatttttaaggcccctgccctttctcccccaacaaggtagttttatagatgaaattttggtatgattttatcaactatttcttaatatatcataacctggatattctgaaaccagtcagtcgataaagacatatatttccacgccctttaaaataattgtttggttttttaaacctattttatagtcatagtctgtgacctgtcaccttttttttcttttaaaatgagctggaaccaggatgcttcctgattagccaggctttgccaatgtataaggcacattgagtgatgctggactttaaaaaaaaagtaccagcatgtaaaaagcttgttttccctgggaattaaggctgtgcacacagctgcttacagtacagttctgtttgcgcttctctcagcctgaaatgtgcttggtggctttgccttcctctttttcagctaaaactgtgtggaagaagttgattttccccctttttctgtagagtctggattccctcacaggaagacaggactccttttcttgtccagcaaagggagttggtacaaagcaagcatcaattagaaactaggggaaatggattctgtatttaatgtagataatactttgtaaaggacaagcgttacatagtctaggtagttccacccagggtttcccagcccacattttactctgtccccaaagggcaaggggtgaaaggtaagaggctccctgaagagccacactccttgagctgaggaaggagctgggaagagacaaacacaatatactgaaaacaattttgttgtctctatctagaaaccattggtgggaaacactggatcagatggtgaagggtgtttgtttgaactcattgattcaagatccaaatctgttaaactagccttctcaaaatcccagcaatacaaagcactggtctccttcagtcttttcagcaagcttgtaaatacattaatgtttacatttccgtgtgctgttgctcagcagtgccaccagcgttagctgaactggtctctgagagagtttttaaatgcccatcgagtactttagctcgtgaggatgagtgtagacatccttaatctctgcagacagtggggaaaaacaagtcctgtggctgaagcacaacgaaggcaggagtgaagagacggctgatactattttttcttttttctttttcttttttttttttttttttgcgaatacctcacttggataatacaaatctggacatgttgatcaatggctggctgccccttttattcacactcattcagggagagcacagaaaatactcttcagcacagccactctggatccaagaggaggaaaaaaaatgggagctttttaggagaatgagga

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