LOCUS       XM_030788702            2831 bp    mRNA    linear   VRT 16-JUL-2025
DEFINITION  PREDICTED: Chanos chanos argonaute RISC catalytic component 3b
            (ago3b), mRNA.
ACCESSION   XM_030788702
VERSION     XM_030788702.1
DBLINK      BioProject: PRJNA564626
KEYWORDS    RefSeq.
SOURCE      Chanos chanos (milkfish)
  ORGANISM  Chanos chanos
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Gonorynchiformes; Chanidae; Chanos.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_044506.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_902362185.1-RS_2025_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.4
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/15/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Chanos chanos"
                     /mol_type="mRNA"
                     /db_xref="taxon:29144"
                     /chromosome="12"
     gene            1..2831
                     /gene="ago3b"
                     /note="argonaute RISC catalytic component 3b; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 40 Proteins, and 81% coverage of the annotated genomic
                     feature by RNAseq alignments"
                     /db_xref="GeneID:115824984"
     CDS             74..2656
                     /gene="ago3b"
                     /codon_start=1
                     /product="protein argonaute-3"
                     /protein_id="XP_030644562.1"
                     /db_xref="GeneID:115824984"
                     /translation="

MEISTTGAVGAQPLFTIPRRPGYGTMGKAIKLLANCFQVDIPKIDVYLYEVDIKPDKCPRRVNREVVDSMVQHFKVTIFGDRRPVYDGKRSLYTAHPLPVAAAGVDLDVTLPGEGGKDRPFKVSMKFLSLVSWHMLHEVLTGRSVPDPLQLDKPINTNPVHAVDVVLRHLPSMRYTPVGRSFFSSPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVPGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYDADPFVQEFQFRVRDEMAEVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQFHTGVEIKVWAIACFATQRQCREEMLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYNGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEVIQDLASMVRELLIQFYKSTRYKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKEYQPGITYIVVQKRHHTRLFCADRNERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADEFQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTRRTMYFA"

     misc_feature    290..574
                     /gene="ago3b"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    602..754
                     /gene="ago3b"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    755..1117
                     /gene="ago3b"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(911..913,956..958,998..1000,1010..1012,1064..1066,
                     1085..1087,1091..1093)
                     /gene="ago3b"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1250..2527
                     /gene="ago3b"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1661..1663,1673..1675,1709..1720,1727..1729,
                     1751..1753,1760..1762,1772..1774,1784..1786)
                     /gene="ago3b"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1865..1867,1871..1873,2081..2083,2495..2497)
                     /gene="ago3b"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

gtcgaaaccggagtggcagagccttgagccggtattagatctctcggatagactcctgacggacaccccatgaatggaaatcagtactacaggagcagtcggggctcagcccctgtttaccataccgcggaggcctggctatggcaccatgggaaaggccatcaagctgctggccaactgcttccaggtggacatccccaagatcgatgtctacctgtacgaggtggatatcaagcctgacaagtgcccgcggagagtcaacagggaggtggtggactccatggtgcagcatttcaaagtgaccatctttggggaccgcagacctgtctatgatggcaagaggagtctttacactgctcatcctctaccagtggccgctgccggggtggatttggacgtgacgttaccaggcgaggggggaaaagaccgcccctttaaagtctccatgaagtttctgtccctggtcagctggcacatgctacatgaagtgctgactgggcggagtgtgcctgaccccctgcagctggataagccaatcaacaccaaccctgttcacgccgtggacgtagtcctgcgccacctgccctccatgaggtacacgccagtgggtcggtcctttttctcctcgccggaaggatacgaccatccgctgggcggtggcagggaggtatggtttggattccaccaatcagtgcggcctgccatgtggaagatgatgcttaacattgatgtgtcagccactgcgttctacaaggcccagcccgtgatccagttcatgtgtgaggttctggacatccacaacatcgacgagcagcctcgtccactcaccgactcccaccgagtcaaattcaccaaagagattaaaggtctgaaggtggaggtgacacactgcggcacgatgcggaggaagtacagagtgtgtaacgtcacacgccggcctgccagccaccaaacgttccccttacagctggagaatgggcagacagtggagcggacggtggctcagtactttagagagaagtacagtcttcagctgaagtatccccatttgccatgcctgcaggtgggccaggaacagaaacacacctacctgcctctggaggtgtgtaacatagtgcccggccagcgatgcattaaaaaactgacggataaccagacttccaccatgatcaaagccactgcacgctctgccccagacagacaagaggagatcagcagactggtgcgcagtgctaactacgacgcggacccgttcgtgcaggagttccagttccgtgtgcgtgacgagatggcggaggtgacggggcgggtcttgccggcgcccatgctacagtatggcgggcggaaccgtacggttgccaccccgagtcatggcgtgtgggacatgagagggaaacagttccacaccggagtggagatcaaagtgtgggccattgcctgtttcgccacacagagacagtgcagagaagaaatgcttaagagctttactgatcagttgcgtaagatctcaaaggatgcgggaatgcccattcaaggtcagccgtgtttctgtaaatatgcccagggtgcggacagtgtggagcccatgttcagacacctgaagaacacctacaacggcctacagctcatcatcgtcatccttcccgggaagactcctgtctacgcggaggtgaagcgtgttggcgacaccctactgggcatggccacacagtgtgttcaggtgaagaacgtggtgaagacatcgcctcaaaccctctccaatctctgcctcaaaattaatgtgaaactaggcggcatcaataatattctggtcccacaccagcgtccgtctgttttccagcagcctgtaattttcctgggtgctgatgtcacgcatcctcctgctggagatggaaagaagccatccatcgctgcggtggtgggcagtatggatgcccatcccagtaggtactgtgccacagtgagggtgcagaggcccagacaggaggtcattcaggatctggcctccatggtgcgggaactcctcatccagttctacaaatccacacgatacaaacccaccaggattatcttctacagggacggcgtgtctgagggacagttcagacaggtgctgtattatgagctgttggccatccgtgaggcctgtatcagtctggagaaggagtaccagccaggcatcacctacatcgttgtgcagaaacgccatcacacgcgcctcttctgtgccgaccgcaatgaaagagttggacggagtggaaacattcctgccggcaccacagtggatacagatatcacacacccgtatgagtttgacttttatctctgcagtcacgcgggcatccagggtacgagcagaccctctcactaccatgttctgtgggacgacaactgtttcacggccgacgagttccagctgctgacctaccagctgtgccacacctacgtccgctgcacccgctccgtctccatcccagcccccgcttactacgcccacctggtggccttccgcgctcgttaccacctggtggataaggaacacgacagtgcggaggggagccatgtgtctggacagagtaacggccgtgaccctcaggctttggccaaggctgtgcagatccaccacgacacacggaggactatgtactttgcctagctccgcctcctcagctgattgagacctgtcactcatccatcaccatgcactgtgctggagaaggctctgtgcctgctgaaaactgattaagacatgccaagctgctataaacaaaaaaaaaagagagaaacaatcacaagaggaaaacagcagggggtgctcaaacactgttatg

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