LOCUS       XM_030788640            2938 bp    mRNA    linear   VRT 16-JUL-2025
DEFINITION  PREDICTED: Chanos chanos argonaute RISC catalytic component 2
            (ago2), transcript variant X1, mRNA.
ACCESSION   XM_030788640
VERSION     XM_030788640.1
DBLINK      BioProject: PRJNA564626
KEYWORDS    RefSeq.
SOURCE      Chanos chanos (milkfish)
  ORGANISM  Chanos chanos
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Gonorynchiformes; Chanidae; Chanos.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_044506.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_902362185.1-RS_2025_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.4
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/15/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Chanos chanos"
                     /mol_type="mRNA"
                     /db_xref="taxon:29144"
                     /chromosome="12"
     gene            1..2938
                     /gene="ago2"
                     /note="argonaute RISC catalytic component 2; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 32 Proteins, and 100% coverage of the annotated
                     genomic feature by RNAseq alignments, including 1 sample
                     with support for all annotated introns"
                     /db_xref="GeneID:115824922"
     CDS             162..2780
                     /gene="ago2"
                     /codon_start=1
                     /product="protein argonaute-2 isoform X1"
                     /protein_id="XP_030644500.1"
                     /db_xref="GeneID:115824922"
                     /translation="

MYSSGAAGAAEMYEGPHTSGSVGSAPPSPSAQEYVFKPPQRPDFGTMGRTIKLQANFFEMEIPKLEVYHYDIDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVYDGRKNLYTAMPLPIGRDKVELEVTIPGEGKDRSFKVAIKWVSCVSLQALHEALSGRLPNIPFETIQALDVVMRHLPSMRYTPVGRSFFTPSEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFMCEVLDFKSIEEQQKALTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQENGQTIECTVAQYFKDKYKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQDEISKLMRSANFNTDPYVREFGVMVRDEMTEVNGRVLQAPSILYGGRNKAIATPIQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTELLLKAFTDQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFKHLKYTYQGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQVKNVQKTTPQTLSNLCLKINVKLGGVNNILLPQGRPLVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQQHRQDIIQDLAAMVRELLIQFYKSTRFKPTRIIYYRDGISEGQFNQVLQHELLAIREACIKLEKDYQPGITFVVVQKRHHTRLFCMDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNQFTSDELQVLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDQQALAKAVQIHQDTLRTMYFA"

     misc_feature    474..698
                     /gene="ago2"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    726..878
                     /gene="ago2"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    879..1241
                     /gene="ago2"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1035..1037,1080..1082,1122..1124,1134..1136,
                     1188..1190,1209..1211,1215..1217)
                     /gene="ago2"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1374..2651
                     /gene="ago2"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1785..1787,1797..1799,1833..1844,1851..1853,
                     1875..1877,1884..1886,1896..1898,1908..1910)
                     /gene="ago2"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1989..1991,1995..1997,2205..2207,2619..2621)
                     /gene="ago2"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

ttgtaattttgtgctcgtgtaaaatcttacttttcccttctaatccactgattttgttttgagcaattggacgcttctgttctcgcatgcatttttgctgcgtttgacgacgttagaggaatcactgtgatattaagggctgctccaagaaaacgaccaaaatgtattctagtggagcagctggtgctgctgagatgtatgagggacctcacacttcggggtcagttggttctgctcccccatctccctcagcacaggagtatgtgttcaaacctcctcagcgaccagattttggcaccatgggcaggacaatcaaactgcaggccaacttttttgagatggagatccccaaactggaagtgtaccattatgacatagacatcaaacctgagaagtgcccacgaagagtcaaccgtgaaattgtcgagcacatggtgcagcactttaaaacgcagatctttggtgatcggaaaccagtgtatgatgggcggaagaatctgtacactgctatgcccttacccatagggcgagacaaagtggagttggaggtgactatcccaggagagggcaaggacagaagtttcaaagttgccataaaatgggtgtcctgcgtcagcctgcaagctcttcatgaagcactgtctgggcggctgcccaacatcccctttgagaccattcaggctctggatgtggtcatgagacatctgccctctatgaggtacacgcccgttggccgctcattcttcacaccttcagagggctgctccaaccctctgggtggaggaagagaggtttggtttggttttcaccagtctgtccgaccatccctttggaaaatgatgctgaacatagacgtttctgcaactgcgttctacaaagcccaacccgtgattgagttcatgtgtgaagttttggatttcaaaagcattgaagagcaacagaaggccttaacagactctcagagagtgaagttcaccaaggagatcaagggtttgaaagtagagatcacacactgtgggcagatgaagaggaaatacagggtgtgtaatgtaactcggagaccggccagccaccagacgttcccattacagcaggagaatggacagaccattgaatgcacggtagcgcagtacttcaaagacaaatacaaactggtgctacgatatccccacctgccctgtttacaggttggccaggagcagaaacacacctaccttcctctggaggtctgtaacatcgtggctggacagcgttgcataaagaagctgacggataatcaaacgtcaacaatgatccgtgccacagccaggtctgcgcccgatcggcaggatgagatcagcaaactgatgagaagtgccaactttaacaccgatccttacgtccgtgagttcggggtcatggtgagagatgaaatgacagaggtgaacggccgcgttctccaagccccgtcgattctttatggaggacggaataaagcaatagccacaccaatccaaggagtatgggacatgagaaacaagcagttccacactgggattgagattaaagtgtgggctatcgcctgctttgccccacagaggcaatgcactgaactcttactcaaagctttcactgaccaactgcgtaagatctctcgtgacgctgggatgcccattcagggacagccctgcttttgtaagtatgcccagggtgctgacagcgtggagcccatgttcaagcacctgaagtacacttaccaaggcctgcagttagtggtggtcatcctgcccggcaagacccctgtttacgctgaagtgaagcgtgtcggtgacactgtattaggcatggccactcagtgtgttcaagtgaagaatgttcagaagaccacaccacagaccctctccaacctctgcctgaagatcaatgtcaagcttggtggagtcaacaatattctcctcccacagggcaggcccttggtgttccaacagccagtcatctttctgggtgctgatgtgacccacccaccggctggagatgggaagaaaccttcaatagctgctgtggtagggagcatggatgcccaccccagccgctactgtgccacagtgcgagtgcagcagcatcgccaggacatcattcaggatctggccgctatggtgagagagctgctgatccagttctacaaatccacccgcttcaagcccacgcgcatcatctactacagagacggcatctcagagggccagttcaaccaggttctccagcatgagttacttgcaattcgtgaggcctgcatcaagctcgagaaagactaccaaccaggcatcacctttgttgtggttcagaagagacaccacactagattattctgcatggacaggaatgagagggttggaaagagtggcaacatccctgcaggcacaacagttgacaccaagatcacccatccatcggagttcgacttttacctctgtagccacgctggcattcagggaaccagcaggccatctcactaccacgtgctgtgggacgacaaccagttcacctctgatgagctgcaggttctcacttaccagctgtgccacacttacgtgcgctgcacccgctccgtgtcaatccctgcaccagcctactatgcgcacctggtggcgttccgcgcccgctaccatctagtggataaggagcatgacagtgctgagggtagccacacatccgggcagagcaacggccgagaccagcaggccctggccaaagccgtgcagattcaccaggacacactgcgcaccatgtatttcgcctgaacaccacccaggatgagctgccattcacacaaagcttttccactaccaagaatacggtgtgtgtgtgtttgtgtgtgtgtgtgtgtgtgtacgcatgtgtgttttcagtgtgtgacccctcagttctgttgtcaaatggcaaatttgaagattatcct

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