LOCUS       XM_024371992            4278 bp    mRNA    linear   INV 10-APR-2020
DEFINITION  PREDICTED: Bombus impatiens protein argonaute-2 (LOC100748831),
            mRNA.
ACCESSION   XM_024371992
VERSION     XM_024371992.2
DBLINK      BioProject: PRJNA70395
KEYWORDS    RefSeq.
SOURCE      Bombus impatiens (common eastern bumble bee)
  ORGANISM  Bombus impatiens
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Apidae; Bombus; Pyrobombus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NT_178641.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Apr 10, 2020 this sequence version replaced XM_024371992.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Name             :: Bombus impatiens Annotation Release
                                           103
            Annotation Version          :: 103
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 8.4
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Bombus impatiens"
                     /mol_type="mRNA"
                     /isolation_source="haploid male brothers"
                     /db_xref="taxon:132113"
                     /chromosome="Unknown"
                     /sex="male"
                     /country="USA"
     gene            1..4278
                     /gene="LOC100748831"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 73 Proteins, and 100% coverage of
                     the annotated genomic feature by RNAseq alignments,
                     including 56 samples with support for all annotated
                     introns"
                     /db_xref="GeneID:100748831"
     CDS             868..3654
                     /gene="LOC100748831"
                     /codon_start=1
                     /product="protein argonaute-2"
                     /protein_id="XP_024227760.1"
                     /db_xref="GeneID:100748831"
                     /translation="

MAQMKQQLPLQMPDLSSLRITTAVSMLGKAYGCGQCSAPPPGPTTSSSVGASGATGSSVVAPGSLGLVSTQQTHTPPQPPELPMFSCPRRPNIGREGRQITLRANHFQITMPRGYVHHYDINIQPDKCPRKVNREIIETMVHAYSKIFGTLKPVFDGRNNLYTRDPLPIGTDKIELEVTLPGEGKDRVFRVVIKWLAQVSLFALEEALEGRTRQIPYDAILALDVVMRHLPSMTYTPVGRSFFSTPDGYYHPLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRDIGDQKRPLTDSQRVKFTKEIKGLKIEITHCGTMRRKYRVCNVTRKPAQMQSFPLQLENGQTVECTVAKYFLDKYKMKLRHPYLPCLQVGQEHKHTYLPLEVCNIVAGQRCIKKLTDMQTSTMIKATARSAPDREREINNLVRRADFNNDSYVQEFGLTISNNMMEVRGRVLPPPKLQYGGRVSSLSGQTKQQAIPNGGVWDMRGKQFFTGVEIRVWAIACFAPQRTVRDDSIRNFIAQLQRISNDAGMPIIGQPCFCKYATGPDQVEPMFRYLKATFSSLQLVCVILPGKTPVYAEVKRVGDTLLGMATQCVQAKNVNKTSPQTLSNLCLKINVKLGGINSILVPTIRPKVFDEPVIFFGADVTHPPAGDNKKPSIAAVVASMDAHPSRYAATVRVQQHRQEIIQELSSMVRELLLMFYKSTGGYKPLRIILYRDGVSEGQFLHVLQHELTAIREACIKLEAEYRPGITFVVVQKRHHTRLFCSEKRDQSGKSGNIPAGTTVDVCITHPTEFDFYLCSHQGIQGTSRPSHYHVLWDDNRFESDELQSLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVEKEHDSGEGSHQSGCSEDRTPGAMARAITVHANTKRVMYFA"

     misc_feature    1159..1548
                     /gene="LOC100748831"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:435368"
     misc_feature    1576..1728
                     /gene="LOC100748831"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:430160"
     misc_feature    1729..2091
                     /gene="LOC100748831"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1885..1887,1930..1932,1972..1974,1984..1986,
                     2038..2040,2059..2061,2065..2067)
                     /gene="LOC100748831"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    2227..3525
                     /gene="LOC100748831"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(2656..2658,2668..2670,2704..2715,2722..2724,
                     2746..2748,2755..2757,2767..2769,2779..2781)
                     /gene="LOC100748831"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2860..2862,2866..2868,3079..3081,3493..3495)
                     /gene="LOC100748831"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

cgcaataactggtgaatcgaacaagtagtggggtctttctcgcggatgttgattactgtttcggtgaatgttgccgaaagtttcccggtaaattaatgaaaaacatacggcgttcgttatgcataaaatataaaagtgcattgaaatactttataacaaagattactgtacatcagtatcggtgtcattgtaaaataagtgcgagcattacgataaaatattattgttttatgtctctactaacataacagaaatgaagagagtgtagagtgtatgcgagtgcattcaacatactcacacaatcggtatattcgacatgttcgtgtacaatggcaaccttgacacttggtgatcgtttttggcggcttcgttccgttcttactgaaatcgtgtttggtaggatcgcgtcgtgagtgaaagcggcggtggccattctgtaaaatcttacagagtttggcagtatcgaatcagtaaaatatttcgcgcttcgtattaacgatattccgttattgtgtttgttttcgtgactgcaatttcgttcgctttataattctcgtgtaaacttttgttctataaagaaatacatcatattacaaaccacaccaaacattttatgcatacattggaaattgagaaaaataaacaattaagataattaattattgtcatgcataataaattattattatattatatttcatcacggaagatgtgtcttttatagtgctatatataaaactgttacacagtgcatagacactaccgtgaagtttctatataaaaaaataatgtttatgtgaattcgataacagtgtttatctattccgcatattataaattgaatactacttctataaaacaggaaaaatggcacaaatgaaacaacaactaccacttcaaatgccagatttaagtagtcttcgcataactacagctgtttccatgcttggtaaagcatatggatgtggacagtgtagtgctcctccacctggacccacaacaagttcttctgttggtgcatctggagcaactggcagtagtgttgtagctccaggttctttaggacttgtttccacacaacaaacacacactcctcctcaacctcctgaactgccaatgttttcatgtcctcgaagaccaaacataggacgtgaaggcagacaaattaccttaagagctaatcacttccaaataacaatgccacgtggttatgtgcaccattatgatattaatatccaacctgataaatgtcctcgtaaagttaatagggaaataatagaaacaatggttcatgcatatagtaaaatatttggaactcttaaacccgtattcgatggcagaaataatttatatacaagggaccccttgcctatcggtacggacaaaatagaattagaagtaacactgcctggtgaaggtaaagatagagtttttagagtggtgataaagtggctggctcaagtttcactgtttgctttagaagaagctttagaagggcgtacaagacaaatcccatatgacgctatacttgctttagatgttgtaatgaggcatttaccatctatgacatatactccagtaggtagatcattctttagtacaccagatggatattaccatccattaggtggtggtagagaagtttggtttggttttcatcaatctgttaggccatcccaatggaaaatgatgctaaacattgatgtttctgcaactgcattttataaagcacaacctgttatagaatttatgtgtgaagtattagacattcgagacataggtgatcaaaagagacccttaacagattctcagcgagttaaatttaccaaagaaattaaaggacttaaaattgaaatcacacactgtgggacaatgaggcgaaaatatagagtgtgtaatgttacgcgtaaacctgctcaaatgcaatcattcccgttacaattagaaaatggacaaacagtcgagtgcacggttgcaaaatatttcttagacaaatataaaatgaaattgcgacacccatatcttccatgccttcaagttggacaagaacataaacatacatatttgccacttgaggtctgtaatattgttgctgggcaacgttgtatcaagaaattgactgatatgcagacttctactatgataaaagctacagcccggtctgctccagatcgtgaacgagaaataaataacttagttagaagagctgattttaataatgattcatatgttcaagaatttggtttgacgatatcaaacaatatgatggaagttaggggtcgtgttttgcctccacccaagctacagtatggagggcgcgtaagttccctcagtggacagacgaagcagcaggctatacctaatggtggtgtttgggatatgcgaggcaagcaatttttcacaggtgtggaaattagagtttgggcaattgcttgtttcgctccacaaagaactgtacgcgatgattcaatacgtaattttatagcacaattacaaagaataagcaatgatgctgggatgccaattattgggcaaccatgtttttgtaaatatgctactgggccagatcaagtagagcccatgtttagatatctaaaagcgacattttcatcattacaacttgtttgtgttatattacctggaaaaacacctgtatatgctgaagtcaaaagagtaggagatacattattgggtatggcaacgcagtgtgtacaagcaaaaaatgttaataaaacgtcaccacagacgttgtcgaatttatgtttgaaaattaatgtaaaattaggtggtattaatagtattctagtacccactatcagaccgaaagtgtttgatgaacctgttatattttttggagctgatgtgactcatcctcctgctggagataataaaaagcctagcatagcggcagtagttgctagtatggatgctcatccatctcggtacgcagctactgttagagttcaacaacatagacaagaaattattcaggaacttagttcaatggtgagggaactccttcttatgttctacaaaagcactggtggatataaaccacttaggataattctttatcgcgatggtgtctcagaaggtcaatttcttcatgttttacaacacgagttaactgccattcgagaagcttgtattaaacttgaagctgagtatagacctggaataacatttgttgtagtccagaagagacatcacactcgcctattttgttcggaaaaaagagatcaaagtggtaaaagcgggaatataccagccggtacaacagttgatgtctgtataacgcatccaactgaatttgacttctatttatgtagtcaccaaggcatccagggcacgtctcgaccatcgcattatcacgttttatgggatgataatcgttttgaaagtgacgaattacagtctctaacgtatcaattatgtcacacgtatgtcagatgtacgagatctgttagtatacctgcaccagcatattatgctcatctcgtagcattccgagccagatatcatttggtagaaaaagagcatgatagcggagaaggatctcatcaatctggctgtagcgaagacagaacaccaggtgcaatggcaagagctatcacagttcatgcaaacacaaagcgggttatgtactttgcataattcttctttgaatttttcttagacacataagcaaaatagtaaaaataataaaaataccataaaatgataatgatcatcatatcttgcacgttgatatttttagactgcttgaaatgctggacaatcttcttgagtggtcaacttttttgcttacttttataatcaaataaatctgcctaatacctaaaaatatgtcgaacaattttatattaagcttttttttcttgtttttttttttttttaatacattgtcgactaggttttggacagattttttaacacgttaggaagtaggtattaaacgaggtgcttttagttaatcatgttttagattgttgcagcttgactgtcggatagtttcgcgataaaaaccacatgaacgaataatttaagaatccgtgctgccgtggatattacgcttatttcttttttttttttttttagaggttgaatttcctctagcatttgtatgatgcaaaaagatcgtattacaacataaattcacgattgtataaatcgcttctgcaagactgttatcttgttgtttatatatagttgtcatttgtatcagtcattttttgtaaaaaggaaaaataaaaaagatgtggactttcaagtcacact

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