LOCUS       NM_001114370            2571 bp    mRNA    linear   INV 30-JUN-2020
DEFINITION  Tribolium castaneum Argonaute-2a (Ago-2a), mRNA.
ACCESSION   NM_001114370 XM_965062
VERSION     NM_001114370.1
KEYWORDS    RefSeq.
SOURCE      Tribolium castaneum (red flour beetle)
  ORGANISM  Tribolium castaneum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Coleoptera; Polyphaga;
            Cucujiformia; Tenebrionidae; Tenebrionidae incertae sedis;
            Tribolium.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Tomoyasu Y, Miller SC, Tomita S, Schoppmeier M, Grossmann D and
            Bucher G.
  TITLE     Exploring systemic RNA interference in insects: a genome-wide
            survey for RNAi genes in Tribolium
  JOURNAL   Genome Biol. 9 (1), R10 (2008)
   PUBMED   18201385
  REMARK    Publication Status: Online-Only
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            AAJJ02000562.1.
            
            On Feb 7, 2008 this sequence version replaced XM_965062.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EU273916.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN03247803 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1443              AAJJ02000562.1     10303-11745         c
            1444-2571           AAJJ02000562.1     9121-10248          c
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Tribolium castaneum"
                     /mol_type="mRNA"
                     /strain="Georgia GA2"
                     /db_xref="taxon:7070"
                     /map="LG3"
                     /linkage_group="LG3"
     gene            1..2571
                     /gene="Ago-2a"
                     /gene_synonym="Ago2A"
                     /note="Argonaute-2a"
                     /db_xref="BEETLEBASE:TC011525"
                     /db_xref="GeneID:658698"
     CDS             1..2571
                     /gene="Ago-2a"
                     /gene_synonym="Ago2A"
                     /note="GLEAN_11525; AUGUSTUS-3.0.2_11525"
                     /codon_start=1
                     /product="Argonaute-2a"
                     /protein_id="NP_001107842.1"
                     /db_xref="BEETLEBASE:TC011525"
                     /db_xref="GeneID:658698"
                     /translation="

MAPLPDGPDPSTKQKQPPTFAPQKESSFSTQKLKSSLSDCLSQTVIIKPGVKGRPIKIESNHLSLNVGTLTEAYHYDVSITPDTPKSFLRDVMNLFARKHYPKNHPAFDGRKNLYSPKKLPLPNDTMSDTIELEGENKKRGFKVVVKLARTVDLSPLRDILQTRQSPQDALQCLDIVLRNAPSNSCISSGRCFFTPPREGQILRLGDGMEMYYGFYQSAIRGWKQPLLNVDVVHKAFPEALNVLDLVCELGSDYRNTMTRQDLNQPLTDFVQKALEKFLKQLKVTYEIPGQSGSRRIFRVNGLRAPPSQARFTLGDGKVTTVEKYYQEVKRCRLQYPHLPTLWVGSRQREVLIPLEFCTVVSGQVVNRKMNENQTSVMIKKAATSTDVRKDKIMQVLRKANYNSDPCVREFGFSVNNSFEKLDGRVLQPPTLLYARKAEVTPSKGVWRADMNRFFVGAIVHKWTIVSCTRHPERGEQLADMIFRMASSNGMQITSKATGPFQHLGGRQNLRDIIDYFKRKQDHDLIIVVVPNSGPQYSLVKQAAELNVGCLTQCIKERTIAKLNPQIIANILLKINSKLNGTNHILSSRLPIMSRPCIIMGADVTHPGPDAKDVPSVAAVTASHDPNAFQYNICWRLQPPKVEIIEDLCAITVEQLMFFYRKTRHKPETIVFFRDGVSEGQFAEVRRAEISAIHQACKKLQREGYEPRITFLVVQKRHHTRLFPTNPRDSEDRNNNVPAGTCVDTHITNPMMQDFYLVSHASIQGVAKPTKYCTLWDDNNMSNDDIEELTYYLCHMFTRCNRSVSYPAPTYYAHLAAARAKVYVENVKLDLTQLKTHQQKCQIQESIVKEKPMFFV"

     misc_feature    310..534
                     /gene="Ago-2a"
                     /gene_synonym="Ago2A"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:435368"
     misc_feature    562..717
                     /gene="Ago-2a"
                     /gene_synonym="Ago2A"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:430160"
     misc_feature    718..1083
                     /gene="Ago-2a"
                     /gene_synonym="Ago2A"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(892..894,934..936,964..966,976..978,1030..1032,
                     1051..1053,1057..1059)
                     /gene="Ago-2a"
                     /gene_synonym="Ago2A"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1216..2469
                     /gene="Ago-2a"
                     /gene_synonym="Ago2A"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1609..1611,1621..1623,1657..1668,1675..1677,
                     1699..1701,1708..1710,1720..1722,1732..1734)
                     /gene="Ago-2a"
                     /gene_synonym="Ago2A"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1807..1809,1813..1815,2023..2025,2440..2442)
                     /gene="Ago-2a"
                     /gene_synonym="Ago2A"
                     /note="active site"
                     /db_xref="CDD:240015"
     exon            1..1443
                     /gene="Ago-2a"
                     /gene_synonym="Ago2A"
                     /inference="alignment:Splign:2.0.8"
     exon            1444..2571
                     /gene="Ago-2a"
                     /gene_synonym="Ago2A"
                     /inference="alignment:Splign:2.0.8"
ORIGIN      

atggcacctctcccagacggtcccgacccatccacaaaacaaaaacagcccccaactttcgccccccaaaaggaatcttcattttccactcaaaaacttaaaagttctttgagcgactgcctttcacaaactgtcattatcaaaccaggagtcaaaggcagacccataaaaatcgaaagcaaccatttatccttgaacgtgggcacactcacagaagcttaccattacgacgtctccatcacacctgacactcccaaaagcttcctccgcgatgtcatgaaccttttcgctcggaaacactatccgaaaaaccacccggccttcgacggccggaaaaatctctactcgcctaaaaaattgcctttgcccaacgacaccatgagtgataccattgaacttgaaggagaaaacaagaaacgggggtttaaagttgtggttaaactggcgagaactgtagatttgtcgccacttcgtgatattttacaaacgagacaatcgccacaagacgctttacaatgcctggatattgtcctaagaaacgctccttctaacagctgcataagctcagggaggtgtttttttacacctccaagggagggccaaattttgcgtctgggggacggcatggaaatgtactacgggttttatcaatcggctatcagaggctggaaacagccgctcctgaacgttgatgttgtccataaggcgtttcccgaagccttgaacgtcctagacctcgtgtgcgaactagggagcgactacaggaacactatgacacgacaagacctcaaccaacccctcactgatttcgtgcaaaaagccttggaaaaattcctcaaacagttgaaagtcacgtatgaaataccgggccaaagtggctcaagacgcatttttcgcgtcaatggcctccgtgcgcccccaagtcaggcccggtttacgcttggcgatggcaaagtcaccacggtggagaaatattaccaagaagtcaaacgctgcagactccagtatccgcatttgccgaccctttgggtgggttcgagacaacgtgaggtcctcataccgttggaattttgcacggtggtgagcgggcaagtcgtcaatcgcaaaatgaacgagaaccaaaccagtgttatgatcaaaaaagcggccacttcgacggacgttcgcaaagataagatcatgcaagttctgcgaaaggcaaattacaatagtgatccctgtgtcagggagttcggattttcggtaaataatagtttcgagaagttggatgggcgcgttttgcagccgccgactctgctgtatgcccggaaggcggaagttacgccgagtaagggtgtgtggagggccgacatgaatcggttttttgtgggggctattgtacacaaatggacgattgtgtcctgtacgaggcatccggagaggggggagcagttggccgatatgatcttcagaatggctagttcgaacggcatgcagatcacctccaaggccactggtccgttccagcacctcggcggtcgccaaaacctgcgcgatatcatcgactacttcaagaggaagcaagaccacgacctcatcatcgtcgtggtccccaacagcggcccccaatactcactggtgaaacaagcggctgaactaaacgtcggctgcctgacccagtgcatcaaagaacgcactatcgccaaactcaacccgcaaataatcgccaacatcctcctcaaaatcaacagcaagctgaacggcacaaaccacatactttcctcccgcttgcccatcatgtcccgtccgtgcatcatcatgggggccgacgtcacccatcccggccccgacgccaaggacgtgcccagcgtcgcggcggtgacggcgtcgcacgaccccaacgccttccagtacaacatctgctggcgcttgcagccccctaaagtcgaaattatcgaagatctgtgcgctattacagtcgaacagttgatgtttttctaccgaaaaactcgtcacaagccggaaacgatcgtgtttttccgggacggggtgtctgagggacagtttgcggaagtgaggcgagcggaaatttccgccatacaccaagcgtgtaaaaaactacaaagggagggctatgagcccaggattacgttcctggtggtgcagaaaagacaccatacgaggcttttcccgacaaatccccgggattcggaggataggaataacaatgttccagccgggacttgtgtagatacgcatattactaatccgatgatgcaggacttttatttggtgtcccatgcgagtatacaaggggtggcaaagcctacgaagtattgtacgctttgggatgataataatatgagtaatgatgatattgaggagttgacatattatttgtgtcacatgtttacgaggtgcaatagatcggttagttatccggcaccgacttattatgcacatttggcggcagcgagggctaaggtttatgtcgagaatgttaagctggacttgactcagttgaagacacatcagcagaagtgtcagattcaagagagcattgtgaaggagaaaccgatgttctttgtttag

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