LOCUS       NM_001114356            2775 bp    mRNA    linear   INV 30-JUN-2020
DEFINITION  Tribolium castaneum Argonaute-2b (Ago-2b), mRNA.
ACCESSION   NM_001114356 XM_968168
VERSION     NM_001114356.1
KEYWORDS    RefSeq.
SOURCE      Tribolium castaneum (red flour beetle)
  ORGANISM  Tribolium castaneum
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Coleoptera; Polyphaga;
            Cucujiformia; Tenebrionidae; Tenebrionidae incertae sedis;
            Tribolium.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Tomoyasu Y, Miller SC, Tomita S, Schoppmeier M, Grossmann D and
            Bucher G.
  TITLE     Exploring systemic RNA interference in insects: a genome-wide
            survey for RNAi genes in Tribolium
  JOURNAL   Genome Biol. 9 (1), R10 (2008)
   PUBMED   18201385
  REMARK    Publication Status: Online-Only
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            AAJJ02001937.1.
            
            On Feb 8, 2008 this sequence version replaced XM_968168.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN02442393
                              [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-821               AAJJ02001937.1     51695-52515         c
            822-1509            AAJJ02001937.1     48466-49153         c
            1510-1793           AAJJ02001937.1     45703-45986         c
            1794-2775           AAJJ02001937.1     42000-42981         c
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Tribolium castaneum"
                     /mol_type="mRNA"
                     /strain="Georgia GA2"
                     /db_xref="taxon:7070"
                     /map="LG5"
                     /linkage_group="LG5"
     gene            1..2775
                     /gene="Ago-2b"
                     /gene_synonym="Ago2B"
                     /note="Argonaute-2b"
                     /db_xref="GeneID:662044"
     CDS             1..2640
                     /gene="Ago-2b"
                     /gene_synonym="Ago2B"
                     /note="GLEAN_13762; AUGUSTUS-3.0.2_13762"
                     /codon_start=1
                     /product="Argonaute-2b"
                     /protein_id="NP_001107828.1"
                     /db_xref="GeneID:662044"
                     /translation="

MGAPQQQQPQRGPLPQQQQQQQQRRPQQQQPPVKSPVPHPEPSSPPRQEPAPPLSGGGDCLSGALVVTPGTKGRRIQIESNHLSLNLGKLTEAYHYDVAITPDTPKCLLRDVMNLFGRKHYPQNHPAFDGRKNLYSPKKLPFPNDTKSDTIEVEGENRKKEFKVEVKLARTVDLTPLHDIMRTTQSPQDALQCLDIVLRNAPSNACIIAGRCFFTPPRDGQIIPLGDGMELYYGFYQSAIRGWKALLNVDVAHKAFPKASNVLDIVCEIGSDFRTTMTRANLSQPLREFVQRDFEKFIKQLKVKYEIPNQSSSKRIHRVNGLGEPPSQAKFKLDDGRMTTVERYYQEVKRCKLQYPHLPTLWVGSRERESKILLPLEFCTVVGGQAINRKMNENQTSAMIRKAATSTDVRKDKIMQTLRTANYNNDPCIREFGFSVSNNFEKLDARVLNPPSLLYADNAQIKPSKGVWRADRNRFLVGATINKWTIASGTRYPSRDADKLADMIFRMASSNGMQITSKATPSTHIGGRQGLRDFIDYFKGKQDYDLIIVVVPNSGPQYSFVKQAAELNVGCLTQCIKERTIGRLNPQTVGNILLKINSKMNGTNHRLSPNSRPLIMKRPCMIMGADVTHPSPDARDIPSVAAVTASHDPNAFQYNICWRLQPPKVEIIEDLCNITVEQLKFFYQKTGFKPESIVFFRDGVSEGQFKQVQRAEIAAIQKACKMLQKDDYEPKITFLVVQKRHHTRLFPTNPRDSEDKNNNVPAGTCVDTHITNPRMQDFYLVSHASIQGVAKPTKYCTLWDDNNMNNDDIEELTYHLCHMFTRCNRSVSYPAPTYYAHLAAARAKVYIENDKLDMSQLKRHQEKCQIQEKIVKGKPMFFV"

     misc_feature    370..594
                     /gene="Ago-2b"
                     /gene_synonym="Ago2B"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:435368"
     misc_feature    625..774
                     /gene="Ago-2b"
                     /gene_synonym="Ago2B"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:430160"
     misc_feature    775..1146
                     /gene="Ago-2b"
                     /gene_synonym="Ago2B"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(949..951,991..993,1021..1023,1033..1035,1087..1089,
                     1114..1116,1120..1122)
                     /gene="Ago-2b"
                     /gene_synonym="Ago2B"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1279..2541
                     /gene="Ago-2b"
                     /gene_synonym="Ago2B"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1672..1674,1684..1686,1720..1731,1738..1740,
                     1762..1764,1771..1773,1783..1785,1795..1797)
                     /gene="Ago-2b"
                     /gene_synonym="Ago2B"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1876..1878,1882..1884,2092..2094,2509..2511)
                     /gene="Ago-2b"
                     /gene_synonym="Ago2B"
                     /note="active site"
                     /db_xref="CDD:240015"
     exon            1..821
                     /gene="Ago-2b"
                     /gene_synonym="Ago2B"
                     /inference="alignment:Splign:2.0.8"
     exon            822..1509
                     /gene="Ago-2b"
                     /gene_synonym="Ago2B"
                     /inference="alignment:Splign:2.0.8"
     exon            1510..1793
                     /gene="Ago-2b"
                     /gene_synonym="Ago2B"
                     /inference="alignment:Splign:2.0.8"
     exon            1794..2775
                     /gene="Ago-2b"
                     /gene_synonym="Ago2B"
                     /inference="alignment:Splign:2.0.8"
ORIGIN      

atgggcgccccccaacagcagcaacctcaacgaggtccccttccacagcagcaacaacaacaacaacaacgtcgtccccaacaacagcaaccgcccgttaaatctcccgttcctcatccagaaccttcgtctcctccaagacaagaaccagcacctcctcttagtggcggaggcgactgtctttcgggagctcttgttgtcacaccaggcaccaaaggcagacgtatacaaatcgaaagcaaccatttgtccctaaatctgggcaaactcaccgaggcgtaccattacgacgttgcgataacacctgacactcccaaatgtttacttcgcgatgtaatgaatctcttcggtcgcaaacactatccgcaaaaccatccagctttcgacggcagaaaaaacctctactctccgaagaaattaccgtttcctaatgacaccaaaagcgatacgattgaagtcgaaggagagaatcgcaaaaaggagttcaaagtcgaggttaaattggcgagaacggtcgatttgacgcctcttcatgacatcatgaggacgacgcaatcgccgcaggacgccctccagtgcctggatatcgtcctccggaatgcaccctccaacgcttgtatcatcgccggaaggtgctttttcaccccaccacgtgacggtcaaataatacctcttggtgatggtatggagctgtattatgggttctaccagtcggcaatcaggggctggaaagcgctcctcaacgtcgatgtcgctcataaagcgtttccgaaggcttcaaacgtcctagacatcgtgtgcgagatcggaagcgattttaggactaccatgacacgagcaaaccttagtcaacccctcagagaattcgtccaaagagacttcgaaaaattcatcaaacagttgaaagtcaagtacgaaatacccaaccaaagtagctccaaacgcatccatcgcgtcaacggactcggcgagcctcccagtcaggccaaatttaagctcgacgatggtcgaatgaccacagtcgagagatactaccaagaggtgaaacgttgcaaactccaataccctcacctcccgactttatgggtgggttcgagggaacgtgagtcgaaaatccttctcccattggaattttgcactgtggtgggtgggcaagccatcaatcgcaaaatgaacgaaaatcaaacgagtgcaatgatcagaaaagcggccacttcgaccgatgtccgcaaagataagatcatgcagactttacgcactgccaattataacaatgatccctgtattcgcgagtttgggttttcggtgagtaacaatttcgagaaattggatgctcgtgttttgaatccgccgagtctgttgtacgccgataatgcacaaattaagccgagtaaaggtgtctggagggcggacaggaataggtttctggttggggctactattaacaagtggacgattgcgtccgggacgagatatccgtcgagagatgcggacaaattagctgatatgattttcagaatggctagttccaacggcatgcaaatcacctcgaaggccactccttctacgcacatcggcggacggcaaggccttcgcgacttcattgactattttaagggcaaacaagactacgacctcatcattgtcgtggttcctaactccggccctcagtattctttcgtgaaacaagcagcagaactgaacgtcggctgtctcacccaatgcatcaaggagcgaaccatcggcagactcaaccctcagacagtcggaaatatcctcctcaaaatcaacagcaaaatgaacggcacgaaccaccgactttcgccgaattcgcgtccgctcatcatgaagcgtccctgcatgatcatgggcgccgacgtcactcatcctagtcccgacgctcgggacatccccagcgtcgcggcggtgacggcgtcgcacgacccaaatgccttccaatacaacatctgctggcggctgcagccacccaaagtcgagattatcgaagatctctgcaatatcacggttgaacagttgaaattcttctatcagaagactggttttaagccggaaagcatcgtgtttttccgagacggcgtctccgagggccagttcaagcaagtccaacgagcggaaatagccgccatacaaaaggcgtgtaaaatgctgcagaaggacgattacgagccgaaaattacgttcctggtggtgcagaaaagacaccacacgaggctgttcccgacgaatccccgggactccgaagacaaaaataataatgttccagcgggaacgtgcgtcgacacccatataaccaatccaaggatgcaggacttttacttggtctcacatgcgagtatacaaggagtggcaaagcctacgaagtattgcactctttgggatgacaataatatgaataatgacgatattgaggagctgacataccatctatgtcacatgtttacgaggtgcaacaggtcggtcagttatccagcaccgacttactatgcgcatttggcggcggcaagggctaaggtttatatcgaaaatgataaactggacatgtctcagttgaaaagacaccaagaaaaatgccaaatccaggagaaaatcgtgaaaggaaaaccgatgttttttgtttaaaggaactggagtactgtttatcatgttggtaaaattttctatgtaaggctttatatctattcaatgtttgtttattaattattatcccaaaaaaattataaaaattatggtaaaataaaaaatgcgttgcgcgtc

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