LOCUS       XM_068221696            5997 bp    mRNA    linear   VRT 08-SEP-2024
DEFINITION  PREDICTED: Danio rerio pbx/knotted 1 homeobox 1.2 (pknox1.2),
            transcript variant X4, mRNA.
ACCESSION   XM_068221696
VERSION     XM_068221696.1
DBLINK      BioProject: PRJNA13922
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_007112.7) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_000002035.6-RS_2024_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/15/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /strain="Tuebingen"
                     /db_xref="taxon:7955"
                     /chromosome="1"
     gene            1..5997
                     /gene="pknox1.2"
                     /note="pbx/knotted 1 homeobox 1.2; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 6
                     ESTs, 2 long SRA reads, 4 Proteins, and 100% coverage of
                     the annotated genomic feature by RNAseq alignments,
                     including 10 samples with support for all annotated
                     introns"
                     /db_xref="GeneID:170445"
                     /db_xref="ZFIN:ZDB-GENE-020123-1"
     misc_feature    1
                     /gene="pknox1.2"
                     /experiment="COORDINATES: cap analysis [ECO:0007248]"
                     /note="transcription start site"
     CDS             866..2203
                     /gene="pknox1.2"
                     /codon_start=1
                     /product="homeobox protein PKNOX1"
                     /protein_id="XP_068077797.1"
                     /db_xref="GeneID:170445"
                     /db_xref="ZFIN:ZDB-GENE-020123-1"
                     /translation="

MMTSPSVSMEDYQEAEQMQVGEPTAGQVEERLEEEEEEEEEEERDEEEEDSGSAPSEPQTPMEVYKSAIYRHPLFPLLALLFEKCEQSTQSSECVTSASFDVDIENFVRNQEKEGKAFFSEDPDLDNLMVKAIQVLRIHLLELEKVNDLCKDFCSRYIACLKTKMNSETLLSGDPGSPYSPSPTQPQNPFTGAMSPQGIVVPASALQQGNVTVTAVNPTQMVTGGTVYQPVTVVTPQGQVVTQTLSPGTVRIQNSQLQLQLNQDLSFFSQEDGSSKAKRGILPKHATNVMRSWLFQHIGHPYPTEDEKKQIALQTNLTLLQVNNWFINARRRILQPMLDASSSDTPKSKKKTPQTRPLQRFWPNSLASSVSQQQVTMEDGTTVTVGVGEDGLQTLSSDGATLAMQQVMMGGHSEDDDDEDDEEDEDTDPSHSDMSRLGLETSDSL"

     misc_feature    1136..1393
                     /gene="pknox1.2"
                     /note="N-terminal of Homeobox Meis and PKNOX1; Region:
                     Meis_PKNOX_N; pfam16493"
                     /db_xref="CDD:465140"
     misc_feature    order(1691..1705,1709..1711,1769..1771,1787..1789,
                     1826..1828,1832..1837,1844..1849,1853..1861)
                     /gene="pknox1.2"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238039"
     misc_feature    order(1697..1699,1706..1708,1835..1837,1844..1849,
                     1856..1858)
                     /gene="pknox1.2"
                     /note="specific DNA base contacts [nucleotide binding];
                     other site"
                     /db_xref="CDD:238039"
     misc_feature    1745..1861
                     /gene="pknox1.2"
                     /note="Homeobox KN domain; Region: Homeobox_KN; pfam05920"
                     /db_xref="CDD:428673"
     polyA_site      5997
                     /gene="pknox1.2"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

gtgacgaagattggcactaaggtaagctgtcactcaaaatcttcttttccgactccctatcaatcaggcgatctgggccgcgcgctaaaaatgcactcggcgcataagatcgagcttccgtgggaggcgcgcggtctggaggcttttgggctcaaatctgatgtttggacggcggtttcgggggaaattttctggctcgacgtacacacacagtcaaggcccagctctttttttaatgatcttttgtagaagtgcgaatggcggcggctctagtggggagggatagcccgatgtcccttagattgattgataggcgattatgggcagccttttattatatttaaagagacagacgcgatatttgaagacgttataagtcggaaaaaactcttgaaggctgagttttagttttttttcgtcatgacgcataattgtaaccgtttactaattgcgtcagaggttttcgcgggcgttcggctcttcgaatgaaaacactgactatggatatgaacatgtagcgattgttgagcggacaaacatggcgcctacagtaatagagagaatgagatacagtcatctggagctgctggagaaatagacatgacgaagatgtgacgagaaattgagatgattaatgagtgaacgcttctgtttttaaatggagagcctttttaggtgttcgaagacataatcactcgcttgctgaaggcgcgttgcagtgcagatccctcacggctatatttaatgatgctgctatttatatcgccgttcgtcgacatcagagtttctattgttgtatcagttatagcggtgctgaagatcagctttatagaatgccccagcgttgtccacggcaacagtaactatgatgacatccccatcggtgtccatggaggattaccaagaggctgaacagatgcaagtgggagaaccgacagcgggacaagtggaggagaggctggaggaagaggaagaagaggaggaggaggaggagagagacgaagaagaagaagacagcggctcggctccgtctgagccacaaactcctatggaggtctacaagtcagccatttacagacacccgctgttccccctgttggcgctgctctttgagaaatgcgagcagtccactcagagctcggagtgcgtgacctcggccagtttcgatgtggatatcgagaactttgtgcgaaaccaggagaaggaaggcaaggctttcttcagcgaggaccctgatttggacaatctgatggtgaaggccatccaagtgttacgaattcacctgctggagcttgagaaggtaaatgacctgtgcaaagatttctgcagccgctacatcgcctgcctcaagaccaagatgaacagcgagactctgcttagcggagatcctggaagcccctattctccatcaccgacacagccgcaaaacccattcactggagccatgagtccacaaggtatcgtagtgccagcatctgctttacaacagggcaatgttactgtcacagcggtcaaccccacacagatggtaacaggtggtacagtatatcaacccgttacagtagtcactccacaggggcaggtggtcacacaaactctgtcacctggaacagtacgaatccagaactcacagcttcagctgcagctcaaccaggatctgagttttttcagtcaggaggacggttcgtctaaagccaagagaggcatccttccaaaacacgccaccaacgtcatgcgttcatggcttttccagcacattggtcacccttatccaacagaagatgagaagaagcagatcgccctgcagactaacctcacgctattgcaggtcaacaactggtttattaacgcccggcgacggatccttcagcccatgttggacgccagctcttcagacacacccaagagcaagaagaagacgccccaaacgcggcctttacagcgcttctggccaaactcactcgcctcttctgtgtcccagcagcaggtcaccatggaggacggcaccacagtaaccgtgggtgtgggggaagacggcctgcagacgctttcatcagatggcgccacgctggccatgcagcaggtcatgatgggaggccacagtgaggatgatgacgatgaagatgatgaagaggacgaggacaccgatccgtctcactccgacatgagcagactgggcctggagaccagcgactcgctgtagtaggactgaaccacacacactcgcacactcacgttagcattgagagagacaacagagcactaagaaggagatgtcccacacaaacacacacacacacacacacctcagctgcacacgtcctccagctgtcaaacagcccgcttggagaacagagaaaagggcttaaagccacacacacaccacaaaacgaccccccctccccacacacacacacacacacacaggtcaaaaaaacaagacacaagcacaagtctctctcacgcaaagcatttctgacacagattagccgagtgcactttttgtatgatactgtaaacttaaagaaagtgttcaaagcacatttcgaaagagctaaaaaaggaaggaaattgatttaaaaaaaccagttgttaatgctggatgattgggttgtgcttatcctgacggtctgggatatttccacaacgagatttcgtctcttgcatgcctgtgtttgctttaatcaggcaccaggaaaaagaaggaactatttttatagtacactgtcacagatgatcggtgccaatatgtttgttttctccgtttgtttgttttttgctctggtttgctatgttttgtaaggagtatttatgtatgaaaaagacttttaacctgctcaccctgcttttttttggatgggtggatttggattgtggtaactacttagtataaagtacacaatgatttgtatgctgaaggcagagttgctgacgatgccttatgtttttacatttcgaatgccacaatgtgagacagtctcatatctgtggaaacttatttccaccaccgaatgaacaaaaatgtaattaattattgttctcagaattgtgaggttatatatatatatatatatatatcacaatttggacttttctgtttctatttaagaattacaaacttgtctcataattttgagtttctctttgaattcttagcttgacttggcctattctgagttgatcttttgcataattcaacgtttattgcgattctaagcattgtagccattctgagtttatcttgcacagttttgagcttattacaattctcagtttatctttgcgctatcctgagtttattttaattctgagtttattttaattctgagtttatcttgcgcaatcctgagtttattttaattctgagtttatcttgcgcaatcttgagtttattttaattctgagtttatcttgcgcaatcctgagtttattttaattttgagtttatcttgcgcaatcctgagtctattttaattctgagcttatctttcgtaatcttgtgcaaatttagattttacctcattcacaattctaagtttatatcttgcgcaattctgattttattgtaattcttagtttatcttgcacatttttgagcttattgcaattctgagttgattgtaattctgagtttcttgcgtaaattttgagttcatctcattcgcaagtctaagtttatcgcttaccaatcctgagtttattgtagttcttagtttatcttgctcaattctgagcttattgcaattctgagttgatctaacgcaatctgagtttgtcataattctgagcccatcttgcgcaattccgagtttattgcaattctgagcttatcttacgcagttctgagtttattgtaaatctaattttatctcttacccaatcatgagtttattttaattctgagcttatcttaagcaattctgagtttgtcgtaattctacgcttatcttgcgcaaattttgagtttatctcattcacaagtctaagttcatctcttacctaatcctgagtttattttaactctgagcttatattacgcagttttgagttaattgtaattctgagtttatcttgcacaatcttgagtttattgtaactcttaggtcatcttgcacaatcctgagtttattgtaattctgagtttatcttgcgcaatcctgagtttattgtaattctgaagttatcttgtgcaatcctgagtttattctaattctgagtttatctagctcaattctgagtttattgtaattctaaggttagcttgtgcaatttcgagtttatcttgcgcaattctgagtccactaaaattctgagcttatcttgtgctgttctaagtttatatcttgtgcagtcctgagtttattgtaattctgagctgagtttatctaatgcgcagttctaaatttatcttttacacaatcctaaattctgaactggcacagtcctgagtttattgtaattctgagctaatcttgcacaaattttaagttcatctcatgcgtaatctagagttgattttaattctgatcttttcttgcaaaatcctaagttttttgcaattgcgagcttatcttgcgcaactctgagtttatttcaagcaaacctgagttctttacaactctttgtctctaaattcaaaaggttttttgtaattatttgtctttcttcattcgacacagttctgaccgaatctcgtaattctgtttatttattgcaattttgaatcccacgattatacgtttatttaatgcaattatgatgtaacccagttcttacgattccaggtttgtatctcacagctcttaatttactgtaatttttttctctcaattttaatctgttcgcttctcacaatttagtttttcctcttgcaattctgagttcctttttgtcctgtttatttattcaaattgagtttatatattgctgttttaactgtatagccattttgaatttctctcgcaatttatttattgtctttttgagctgatctctcgcaagtttatttatatcttgcaattctgagttttttcccaattacaccacaatttaataaataccacaattttctttttttcttctcagaattgttcgcttgtatctcacaattcctgtatttctcagaactgaaaaataataattgtgatgggaatgtggtggaaataagcttccatacatatctgatgttacttcctgtgcaatcgacatgaataccgtggccaaaaaaacgatgcatttgcaaggttgtgataatgtttatgatataaatgcctgtacttgttatatgagaaggaactgttatcgctttttaatgatcatattgctgaaaactcccaacagaagcggaagagagaaggacgtcgatcagactttgtatttatgtatatttttttgactcacgagggactcgtctttgatgttaaacctctttagcgcgaacattaatgatggatttctttttaatctgcgttatcatggacaattctcacgtttttcaccatgaaatcgtggataaaacaaaacattttcaacatttaagaattgactgcatgtgctttcatttattattgtatttttagataccgtatgctcacaatctatcagaataaaccccatgagataccaaaataagctgatatatttggattatgcgtctattttgtcccgttttgcgattctacagctgtcacttacttaagctcatgtatccctgttatttagcgtgttcagaatgtgctttaggcagtctgtactgagcggcttttcatttccagctgtagtcttgtgaatgtgatgccatcagtcagatgactagctaacccaaaaggagacagtcggctccgttagcttagcatggggaggttttcaacgctgcactttttctctctgcgcttctataatcgcttctctcacacacactcacaaactcacacacacacagatttgcctttcagactttttgttttataataaagaacactgtacctgaaggccaca

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