LOCUS       XM_021472515            4981 bp    mRNA    linear   VRT 12-MAY-2025
DEFINITION  PREDICTED: Danio rerio shisa family member 8b (shisa8b), transcript
            variant X1, mRNA.
ACCESSION   XM_021472515
VERSION     XM_021472515.3
DBLINK      BioProject: PRJNA1248012
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_133187) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On May 12, 2025 this sequence version replaced XM_021472515.2.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_049306965.1-RS_2025_04
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 04/11/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /strain="Tuebingen"
                     /isolation_source="Zebrafish international resource
                     center"
                     /db_xref="taxon:7955"
                     /chromosome="12"
                     /sex="male"
                     /tissue_type="Fibroblasts and whole tissue"
                     /dev_stage="adult"
                     /ecotype="United States"
                     /geo_loc_name="USA"
                     /collection_date="2022-07-19"
                     /collected_by="National Human Genome Research Institute"
                     /genotype="Double heterozygous"
     gene            1..4981
                     /gene="shisa8b"
                     /note="shisa family member 8b; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 411
                     long SRA reads"
                     /db_xref="GeneID:101882404"
                     /db_xref="ZFIN:ZDB-GENE-130603-87"
     misc_feature    1
                     /gene="shisa8b"
                     /experiment="COORDINATES: cap analysis [ECO:0007248]"
                     /note="transcription start site"
     CDS             230..1525
                     /gene="shisa8b"
                     /codon_start=1
                     /product="protein shisa-9"
                     /protein_id="XP_021328190.1"
                     /db_xref="GeneID:101882404"
                     /db_xref="ZFIN:ZDB-GENE-130603-87"
                     /translation="

MKILALKKQSMAGNYFLLHLLLTAFLGRAAKAQDETSTDMDIQTANHTVDPLMISVTSDGPPTLTTPGEVEEDTVPSTGTRCRGYYDVMGQWDPPFNCNAGIFLYCCGTCFYRFCCQFRQQRLDQTSCSNYDTPIWANTGKPVATNTEVQPDHERDRTHMIVYIICGVVAIMVLVGIFTKLGLEKSRGGQNDLSNSRTLQELMKQPGGEANAVDGPLSNSSSIGANGISARMLRSRSEQYHLNNAGFSFGALAGLSHPQSNLSIPGLALNKYNSLKAVADTANRSYYKSYPLMDFSQHHSPPVAFQPMPMHPKDKSYLHQVLPSHDIHSPLSISIPTNHMERTRIPKTITHPQLSSSAFKAWDSGRRHVHRQTSHPGHLPRRQTYSSRRQYSIETIPDFLSQPTGYGGQPLYQHHPKIKAYQTNSKTEVTV"

     misc_feature    473..>850
                     /gene="shisa8b"
                     /note="Wnt and FGF inhibitory regulator; Region: Shisa;
                     pfam13908"
                     /db_xref="CDD:464036"
ORIGIN      

acaaggctcagaggcagcactctgagtggatcgctgtggattaaaacacctgtctgtgggttcaatcattcttaaaaaaggaatttttatggcaaattactgtgactggttgtttacagaggtgaagaggaaggaagttttactactggagagcacaaagaacaagttttgaaccacatggggaaaacaaaataacgcatttctggacaggaccagagtaagacaacaaatgaagattttagctctcaaaaaacaaagcatggctggaaattattttttactacatcttcttctcactgcctttctgggacgagctgctaaagcacaggacgagacttcaactgacatggacatccaaacagccaatcataccgtagaccctttgatgatctctgtgacatcagatggacctccaactttgaccacacctggagaggtggaggaggacaccgtcccatccactggcacccgttgccgtggttactatgatgtcatgggccaatgggaccccccttttaattgtaacgctggtatcttcttgtactgttgcggcacctgtttctaccggttctgctgccagttccgtcaacagcgactggatcagacctcctgctccaactatgatactccaatttgggcaaatactggaaaacctgtggccaccaacactgaggtgcagccagaccacgagcgggatcgaactcatatgattgtatacattatctgtggtgtggtggccatcatggtcctggtgggcatcttcaccaaactgggtctggagaagagccgtggaggtcagaatgacctaagtaactccaggactctccaggagttaatgaagcagccaggtggagaggcgaatgctgtggatggacctctgagcaacagctcctctattggagcaaatggcatctctgccaggatgcttcgcagccgcagtgagcagtaccatctaaacaacgctggcttttcttttggtgcactggctggactctctcatcctcaaagcaacctcagcataccaggccttgcactcaacaaatacaactctctgaaagcagttgctgacactgcaaaccggagctactacaagagttatccactgatggacttctcccagcatcattcccctcctgtagctttccagccaatgccgatgcacccaaaagacaagtcctaccttcaccaggtcctcccttcccatgacattcactctcctctttccatctcgatcccaaccaaccatatggagaggacccgcatccctaaaaccatcacccaccctcaactgtccagctcggcctttaaagcttgggattctgggcgaagacacgtccaccggcagacctcacatccagggcacctgccgcgacgacaaacctacagcagcagaagacagtacagcatagaaacaataccagatttcctttcccagcctacaggctatgggggacaaccactgtatcagcaccatcccaaaattaaggcctaccagaccaacagcaaaacagaggtcacagtttgaggactcagataactgtatgcaccaacattaccatgtccagaaagtgtggttgttcttactgtcattgctaaaccacttttgtctaagtttcttgttttttttctgtgtcccacttttctgggttcatctaaggcctttgtgacgctgacaacaatgaggtgagctttcagaccggaacggaagaatggcacctgaggtcacattaaattaaataggacaacaccatcaacacatcagcctaaatctaattatcctggccatttttatgtaaacacaacctcaggatcaattatgtgaagtacgaagagacagaatgcaacacctagacatctagtcggtgaggcagtggcggggtaggtagtgctgtcgcctcacagcaagaaggtcgctgagtgctggttcgaacctcggctcatttggcgtttctgtgtggagtttgcatgttctccctgccttcgcgtgagtttcctctgggtgctccggtacaggatgtggtacaggtgaattaggtaagctaaattgtccgtagtgtatgagtgtgtgtgtgtgcgtgtgtggatgtttcgcagagatgggttgcggctggaagtgcatctgctgtgtaaaaacttgctggatgagttggtggttcattccgctgtggcgaccccggattaataaagggactaagccgacaagaatatgaatgaatgagtgagacatctagtcttgcactaggattcaagctttgctaagagtgctgaggttttttggggtgaaaacaaattaaagggtctttcacactggtttgggcagtctcattttaagaaagttcccattaaataactcatttgttttaagaaaatgaggacaaagtacacggcataataatccagtaagggttacaatcacctttgtgcaaccatgagccagtaataatgaggcaaacttgacaataaatctgataaagcaccataagatgctaaacggacattcctaaaatgctataaactgtcttgatacatttctttttacatttgttttgggacaaaaaaagcaaagtcggattggattgggaaaatactgcaaactgggaatcactggaagtaacctaaagcacagctacacaacatgccataaattgttgcacacatggctttcagatccttcctcaatgtacttctgtgaaaatataagcgaaaaaggtcttgttttatgggttttgatggttctcatttaaatctcatatgcagaggacatcaattgtacacctttttatttaaagctcttgtttgcttaagagtctgatctcctctgtttgtcaagactgcgtgtggagtcctagctgtcaagttttacaaattgttgaccaaggatgatttcctcatgacgtgaggctgaagaaatgacgccagcagctttgtgcaataatctggtcattggaagtgggaatgaaacacataactcttataaaacaacctatgcaatggcaaaaatatcagctctagcaaattaatcatccttgatgtcaagatcacacaaggtctctcattgcattatgtaccttttacttgtctgtctttccttttccttggataaatggagagtaaatttccagacatgttatgtatttaaatagacgcacaagatcaaaccatctgctgtaggatcaggagattcagatacacagtgtagcaggattgggaaatctaaaatgctttaaaggggaaatcattgttacaccactttacacacatacagtatactgttacttgtttatcttttgcataaaagcaatatcacacttacattcgtgctgtgacattgaatatcagcagagttgtgaggccttgtgcttacattcagaccaacagcatacttgctatagtgtgatatttcttaaacgtttgtagtattgaaaatgtgcgtaaaaatgtgcttccttattagttcctgtactgcgttgtgctttgcttttattcaaccctgatattcatatgaacttgacaacctttctgttttattatatttatattacgcatatgaattatatttctgtgttcatgtacatgaagttatttaagaattatataagagtctgatttaataattacacagatgctgtgttcagaaatgttttaagatgtcttaaatacgagtgtttgtacgttggtaatgcctgcagtattgtggaaaaacagtgatattcataaactagttgtataagggcttttggcaacaatttgaagaactgaaaattactcaaatttattttgataaaatatattttatattcacattttggctaaataaaagattacaaaaaaaaaactaaacttgttgtaaaacaaatgaaatcgacgtttaacgagttttcacatgtggatttgtacctcaaatgtatgtcacttttcaaaataaaagttctcaaacgtcgaaatgctgtgtattttatacattttacatttttaatatgtaggtaatttccaacaaactacttaccagacagctgggttatgaaagaagcttatatcgtatcactttaatatttacagcgcatgccaatttctggcctgaagaactctagcgccatctgatggtcaccaataacagaaacagcacagagattttaccaacttatatttttgtcctatatcgttatacatgtgttattaaatgtaatatgtttaatagcaatgtttcgttaattaaaaaagttgtaatacatacatagcctatttttaatattaaaattaattatttgacaaaacaatttcaatgtctttttccctaaaaaacgaaatcccttgagaagagagtatttatttactcccacactacatatttacagcagtcaagaaagccataaagttaccctcaatgaggacatctaacaaaacaagtattccaataaatgcctctaaggacttttgcagacatcacacagaaataaagtaaaacaggttagcaataagtgaagctgttgatgctgtttgtggattggtttaaagttcctgttgagctaagattgagatatttcagttttcagatgagccaggagtgtctaaacgtgggactggagggtgtcttgcatagtttagttccagccccaaataaacacacctgaaccaggtaatcaagctctatgtacactataccttccaggcaggtctgttgaaggaagttggagctaaactatgcaggacaccgtccctcaaggattgagtttgggcacccttgatctagggctatctccacacatatttgtaatgtgcatgtatttttgtaaatgaaaaaggatggaaaagactctgaagcttttgaaaatcttgatcctgacaagaattttcaaatgttttgagaacagcagaacttaacgttgtgactcactgaagtcagttgtagttttgtatttttttttcaatgattctacttgttaacagcaggagtacatcattaaaattcatgctgcacttt

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