LOCUS       XM_005158472            5745 bp    mRNA    linear   VRT 08-SEP-2024
DEFINITION  PREDICTED: Danio rerio argonaute RISC component 1 (ago1),
            transcript variant X1, mRNA.
ACCESSION   XM_005158472
VERSION     XM_005158472.5
DBLINK      BioProject: PRJNA13922
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_007127.7) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 8, 2024 this sequence version replaced XM_005158472.4.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_000002035.6-RS_2024_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/15/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /strain="Tuebingen"
                     /db_xref="taxon:7955"
                     /chromosome="16"
     gene            1..5745
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /note="argonaute RISC component 1; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 1 EST,
                     45 long SRA reads, 5 Proteins, and 100% coverage of the
                     annotated genomic feature by RNAseq alignments, including
                     13 samples with support for all annotated introns"
                     /db_xref="GeneID:570775"
                     /db_xref="ZFIN:ZDB-GENE-110606-3"
     misc_feature    1
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /experiment="COORDINATES: cap analysis [ECO:0007248]"
                     /note="transcription start site"
     CDS             335..2950
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /codon_start=1
                     /product="protein argonaute-1 isoform X1"
                     /protein_id="XP_005158529.2"
                     /db_xref="GeneID:570775"
                     /db_xref="ZFIN:ZDB-GENE-110606-3"
                     /translation="

MEAGPSGAVPVGPYPPPLQQVFQAPRRPGMGTVGKPIKLLANYFEVEIPKMDVFHYEVDIKPDKCPRRVNREVVEYMVQHFKPQLFGDRKPVYDGKKNIYTVLALPIGSEKVDFEVTIPGEGKDRIFKVSIRWLAKVSWRLLQETLVSGRLQVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKTLTDSQRVRFTKEIKGILQKHSPGVSFVGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNANFNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKSERIGKSGNIPAGTTVDTSITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHVSGQSNGRDPQALAKAVQIHHDSLRTMYFA"

     misc_feature    335..2944
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /note="protein argonaute; Provisional; Region: PLN03202"
                     /db_xref="CDD:215631"
     misc_feature    1544..2821
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1955..1957,1967..1969,2003..2014,2021..2023,
                     2045..2047,2054..2056,2066..2068,2078..2080)
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2159..2161,2165..2167,2375..2377,2789..2791)
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /note="active site"
                     /db_xref="CDD:240015"
     polyA_site      5745
                     /gene="ago1"
                     /gene_synonym="si:ch211-119n15.3"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

ctgagggggggtgacggacgggtcgagcgttttgttccgctggtgaatcctgcttgaacggaaagcaggagacacaacaacccgacacacagaagccagaccagccactgacgaaagtgcacggaggagcgggtctatataaataggaactacggagcttttgtttctctctcaactcctgcgggtttgggggctccagcggcggcggcggagaagatcccgctcgcctagggaaggaggctggaagtcggggacgcttccagcctgcagggccaggcgccgtggcgattgagtatctggactttggtgaacccttaggggctcagtggatgggatggaggcgggaccatctggcgctgtgcccgtcgggccgtaccctccgcccctgcagcaggtgtttcaggcgccccgcaggcccggcatgggcaccgtcggcaaacccatcaaactgctggcgaactacttcgaggtggagatccccaaaatggacgtgtttcactatgaggtggacatcaagcctgataagtgccctcgacgggtcaacagggaggttgttgaatatatggtccagcatttcaagccgcagctcttcggtgaccgaaagccagtgtatgatgggaagaagaatatctacactgttttagcgttacctataggcagtgaaaaggttgattttgaggtgaccattcctggcgaggggaaggatcgaatctttaaggtgtcgatccgctggttggcgaaggtgtcctggaggctcctgcaggagacgctggtcagtgggcgactgcaggtcccgctggagtccgttcaggctctcgacgtggccatgagacacttggcctccatgaggtacactccagtgggccgctccttcttctcgcctcctgaaggttactaccatcctctaggtggcggcagagaggtgtggttcggcttccatcagtccgttcgacccgccatgtggaagatgatgctcaacattgatgtatctgcaacagccttttataaagctcagcctgtcattgagttcatgtgtgaagtcctggacatccgtaacattgatgagcagcccaagacactaactgactcgcagagggtccggttcaccaaggagatcaaaggaattctgcagaagcacagcccaggcgtatcttttgtcggcctgaaggtggaggttacgcactgtggtcaaatgaaaaggaagtaccgggtctgtaacgtcacacggcgtcctgctagtcaccaaacgtttcctctacagctggagagcggacagacggtagaatgcacagtagctcaatatttcaagcagaagtacaacctgcagctcaagtaccctcacctgccctgtctacaggtgggacaggagcagaaacacacatacctgcctctggaggtttgcaatattgtagcagggcagcggtgcatcaagaaactgacagataatcagacgtccaccatgatcaaagccacggcgcgctccgcaccagaccgacaggaggagatcagcagactgatgaagaacgctaactttaatctggatccttacattcaagagtttgggattaaggtgaaggacgacatgacggaggtgaccggcagagtccttccagccccaatcctgcagtatggcggtcggaatcgtgccatcgcgactccaaaccagggtgtgtgggacatgaggggaaaacagttctataatggcatcgagatcaaggtttgggccattgcttgcttcgctccgcagaaacagtgtcgggaagaggtgctcaagaacttcaccgaccagctgcgcaagatctcaaaggatgctgggatgccgattcagggtcagccgtgtttctgtaagtacgctcagggggccgacagcgtggagcccatgttcagacacctgaagaacacttactctggactgcagctcatcattgtcatcctgcctgggaaaacccccgtctacgcggaagtgaagcgtgttggagacactcttctaggaatggccactcagtgtgtgcaggtgaagaacgtggtaaaaacctccccgcagacgctctccaacctctgcctcaaaatcaacgtcaaactcggcggcatcaacaacatcctcgtgccgcatcaacggtcagcagtgtttcagcagcccgtgatcttcctcggggcagatgttacacacccgccggctggtgatgggaagaagccatctattactgctgttgttggcagtatggacgctcatcccagcagatactgcgctacagttcgagtccagcggcccagacaggagattattgaagatctgtcctacatggtgagagaattactgatccagttctacaagtccacccgcttcaagcccacgagaatcatcttctacagggacggcgtaccagaaggacagctgccacaaatcctgcactatgagctgttggccatcagagatgcctgcattaagctggagaaagactatcagcccggcatcacttacatagtcgtgcagaaacgccaccatacacgactcttctgcgccgataaatctgaacgaatcggcaagagcgggaacataccagcgggaactacggtcgacaccagcatcacacatccattcgagtttgacttctacctgtgcagtcatgcaggcattcagggcaccagccgaccctcacactactacgtcctgtgggacgacaatcgctttacggctgacgagctgcagattctcacctaccagctgtgccacacgtatgtgcgctgcacccgctccgtctccatccccgcgccagcatactacgccagactcgtggctttccgggcccgataccatctggtggacaaagaacatgacagcggggagggaagtcacgtctccgggcagagcaatggtcgagatcctcaagcgttggccaaagcggtgcagattcaccacgactccctgaggaccatgtattttgcctgaataacaaaaagcgcacgtctccggacacctcgagccagaacccctgggtgctaaaccagtccaatgaagcccacacaactgaacactttcttgagtctggcagaactatgctctttcggtttttatttaattgtgtattattgtatactaccttcgcactcccacatgtacgtatgtatgcaatacggagggaaggaacgcaggccaacctgtcgtttttgtatgccaccacaaagctgcctctgaagcgtccgttcgtctagccgtccgggtgccgccgtccagtggagcaatcctcgtttcttccatttggcattatttttattgttattgtgaaattcttttacatttgtattattattttttgtaagaaagagagagagagacatggtacattagcacttagttttagttgaaatggcagcgtttgcttgtcgtctgtgaagcagaacgcgatcaaaactgtagcagtgatgcaggattttcctccagcggttgtgtttagttagttaaagcgtggcctaattagtgtaacgatagatggaccactgagttgcaggatgcactcctgcatcagcataacaccgaaaccagcattatgcaccagccaaaatgtgacgccaaccgctctcatctggaagacgtgggtttttctgcacttgattcctctgcgctcttttagtagaggaggatattgttcactcctgatgcacgcaaatcttcctgagcctaaaagaacacaacttttttggaactaggctcattttacatccctccaagtgttaaacggctgagtttacccatttttaaatccctttagctgattcacttttagcttagcttagcataaatcattgagtcggattagaccattagcatcacagtcaaaattggccaaatagtttggataattgctttatttaaagctctgcgagtgttaaactggtgagttttacaattattaatctctttagcttattcacttttcgcttagcttagcatagatcattaaattggaatagaccattagcatctcactcaaaaatggccaaatagtttggatatttgctttgtttaaagctctccaagtgttaaaaggatgagttttatcatttttgaatctctagctaattcactttgagcttagcttagcatagatcattgaatcggattagaccattagcatctcactcaaaaatggccaaaaagtttggataattgctttatttaaagctctccaagtgttacactgcgagtttcaccattttcgaatccttttatctgattcactttagcttagcttagcatagatcattgtattgaattagaccgttagcatctcagtcaaaaatggccaaatagtttggataattgttttattttaagctctcagagtgttaaacgggtgagttttactattattaatctctttagcttattcacgttcagcttagcttagcatagatcattgaatcggattagaccattagcatctcactgaaaaatggccaaatagtttggataattgctttatttaaagctctccaaatgttacacgatgagtttcaccatttttaaatccctttatctgattcactttagcttagcttagcatagatcattgaattgaattagatcattagcatctcactcaaaaatggccaaatagtttggatatttgtcttatttaaagcttcactcttttgaagttacatcatgtactgagcaactgacgtaaaatcaaaggttattttctgtgtattagcattgcgctgcaataactatggtatggcagcaaagttccttgattattacaccagaatgagagtaaactgtagttcctaaccatttcagcacagaaaacgagcaacttttttttttctgttggtcatagtacatgatgtaactacagaaaagtctttaaaactttaaatttggtcattttttgagatgctaatagtctaatccgattcaatgaactatgctaagctaaaagtgctcctgccagaatctgagatcggctgaatggaataaaaaacagtaaacactcaaatgaaactcaattaaaactcaatactttaactctcagggagttgtaaaatgagcctattttattttttaaaaagtggagtgttcctttaaaacaatagttcacccaacattggcaatattgtcattatttactcacatttatgtgtgtgtttttttaatgcaacacaaaatatttaaagcacctttttttttttataaaaatacaccagcataacctattaaacattttctgaagtcatattttagctttctaaacgcttgttatgcactgttttaatacttgcattcttgcatttcacgtggttgcaaatgagaaaacttcagtgaaagagatgataattcatttagtaatgacttaatgttttggtcagttcctcatctaactctgacaattcagaagacttggaatcactcatacatgcgcacacacagtaaaaagctcctagttgacttcacacaaaaaagtgagtaaacccaatataacttggaactgtaaagttaacttaattagggtttactcccttttttgagttaagtcaactaattgatttttaccgtttgtatattatacaccgaaaaaatgatatgatcaattagtcaagacagcaaatgtttttagttcattgtaagtcaataaactaagttaatcatgttttaacgtaattgtataagttacacaagctgttttaagccagtttatcatcatataaattcaatggactcataaggttgatttgattcaa

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