LOCUS       NM_131364               2780 bp    mRNA    linear   VRT 19-JAN-2025
DEFINITION  Danio rerio MDM2 proto-oncogene (mdm2), transcript variant 2, mRNA.
ACCESSION   NM_131364
VERSION     NM_131364.3
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Flentke,G.R., Wilkie,T.E., Baulch,J., Huang,Y. and Smith,S.M.
  TITLE     Alcohol exposure suppresses ribosome biogenesis and causes
            nucleolar stress in cranial neural crest cells
  JOURNAL   PLoS One 19 (6), e0304557 (2024)
   PUBMED   38941348
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Prykhozhij,S.V., Ban,K., Brown,Z.L., Kobar,K., Wajnberg,G.,
            Fuller,C., Chacko,S., Lacroix,J., Crapoulet,N., Midgen,C.,
            Shlien,A., Malkin,D. and Berman,J.N.
  TITLE     miR-34a is a tumor suppressor in zebrafish and its expression
            levels impact metabolism, hematopoiesis and DNA damage
  JOURNAL   PLoS Genet 20 (5), e1011290 (2024)
   PUBMED   38805544
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2780)
  AUTHORS   Madakashira,B.P., Magnani,E., Ranjan,S. and Sadler,K.C.
  TITLE     DNA hypomethylation activates Cdk4/6 and Atr to induce DNA
            replication and cell cycle arrest to constrain liver outgrowth in
            zebrafish
  JOURNAL   Nucleic Acids Res 52 (6), 3069-3087 (2024)
   PUBMED   38321933
REFERENCE   4  (bases 1 to 2780)
  AUTHORS   Lin,S.J., Vona,B., Lau,T., Huang,K., Zaki,M.S., Aldeen,H.S.,
            Karimiani,E.G., Rocca,C., Noureldeen,M.M., Saad,A.K., Petree,C.,
            Bartolomaeus,T., Abou Jamra,R., Zifarelli,G., Gotkhindikar,A.,
            Wentzensen,I.M., Liao,M., Cork,E.E., Varshney,P., Hashemi,N.,
            Mohammadi,M.H., Rad,A., Neira,J., Toosi,M.B., Knopp,C., Kurth,I.,
            Challman,T.D., Smith,R., Abdalla,A., Haaf,T., Suri,M., Joshi,M.,
            Chung,W.K., Moreno-De-Luca,A., Houlden,H., Maroofian,R. and
            Varshney,G.K.
  TITLE     Evaluating the association of biallelic OGDHL variants with
            significant phenotypic heterogeneity
  JOURNAL   Genome Med 15 (1), 102 (2023)
   PUBMED   38031187
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2780)
  AUTHORS   Sun,K., Han,Y., Li,J., Yu,S., Huang,Y., Zhang,Y., Reilly,J., Tu,J.,
            Gao,P., Jia,D., Chen,X., Hu,H., Ren,M., Li,P., Luo,J., Ren,X.,
            Zhang,X., Shu,X., Liu,F., Liu,M. and Tang,Z.
  TITLE     The splicing factor DHX38 enables retinal development through
            safeguarding genome integrity
  JOURNAL   iScience 26 (11), 108103 (2023)
   PUBMED   37867960
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2780)
  AUTHORS   Woods,I.G., Wilson,C., Friedlander,B., Chang,P., Reyes,D.K.,
            Nix,R., Kelly,P.D., Chu,F., Postlethwait,J.H. and Talbot,W.S.
  TITLE     The zebrafish gene map defines ancestral vertebrate chromosomes
  JOURNAL   Genome Res 15 (9), 1307-1314 (2005)
   PUBMED   16109975
REFERENCE   7  (bases 1 to 2780)
  AUTHORS   Langenau,D.M., Feng,H., Berghmans,S., Kanki,J.P., Kutok,J.L. and
            Look,A.T.
  TITLE     Cre/lox-regulated transgenic zebrafish model with conditional
            myc-induced T cell acute lymphoblastic leukemia
  JOURNAL   Proc Natl Acad Sci U S A 102 (17), 6068-6073 (2005)
   PUBMED   15827121
REFERENCE   8  (bases 1 to 2780)
  AUTHORS   Berghmans,S., Murphey,R.D., Wienholds,E., Neuberg,D., Kutok,J.L.,
            Fletcher,C.D., Morris,J.P., Liu,T.X., Schulte-Merker,S.,
            Kanki,J.P., Plasterk,R., Zon,L.I. and Look,A.T.
  TITLE     tp53 mutant zebrafish develop malignant peripheral nerve sheath
            tumors
  JOURNAL   Proc Natl Acad Sci U S A 102 (2), 407-412 (2005)
   PUBMED   15630097
REFERENCE   9  (bases 1 to 2780)
  AUTHORS   Song,H.D., Sun,X.J., Deng,M., Zhang,G.W., Zhou,Y., Wu,X.Y.,
            Sheng,Y., Chen,Y., Ruan,Z., Jiang,C.L., Fan,H.Y., Zon,L.I.,
            Kanki,J.P., Liu,T.X., Look,A.T. and Chen,Z.
  TITLE     Hematopoietic gene expression profile in zebrafish kidney marrow
  JOURNAL   Proc Natl Acad Sci U S A 101 (46), 16240-16245 (2004)
   PUBMED   15520368
REFERENCE   10 (bases 1 to 2780)
  AUTHORS   Piette,J., Neel,H. and Marechal,V.
  TITLE     Mdm2: keeping p53 under control
  JOURNAL   Oncogene 15 (9), 1001-1010 (1997)
   PUBMED   9285554
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL935183.6 and AL929141.7.
            
            On Aug 24, 2018 this sequence version replaced NM_131364.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC092706.1, EE307258.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA3505370, SAMEA3505372
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                AL935183.6         1192-1229           c
            39-110              AL929141.7         179740-179811       c
            111-185             AL929141.7         179581-179655       c
            186-319             AL929141.7         177991-178124       c
            320-369             AL929141.7         177634-177683       c
            370-428             AL929141.7         177464-177522       c
            429-498             AL929141.7         177239-177308       c
            499-656             AL929141.7         174515-174672       c
            657-806             AL929141.7         174253-174402       c
            807-875             AL929141.7         174074-174142       c
            876-2780            AL929141.7         170970-172874       c
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /strain="Tuebingen"
                     /db_xref="taxon:7955"
                     /chromosome="4"
                     /map="4"
     gene            1..2780
                     /gene="mdm2"
                     /gene_synonym="sb:eu923; zgc:109834"
                     /note="MDM2 proto-oncogene"
                     /db_xref="GeneID:30637"
                     /db_xref="ZFIN:ZDB-GENE-990415-153"
     exon            1..38
                     /gene="mdm2"
                     /gene_synonym="sb:eu923; zgc:109834"
                     /inference="alignment:Splign:2.1.0"
     exon            39..110
                     /gene="mdm2"
                     /gene_synonym="sb:eu923; zgc:109834"
                     /inference="alignment:Splign:2.1.0"
     CDS             51..1478
                     /gene="mdm2"
                     /gene_synonym="sb:eu923; zgc:109834"
                     /EC_number="2.3.2.27"
                     /note="isoform 2 is encoded by transcript variant 2; E3
                     ubiquitin-protein ligase Mdm2; double minute 2 protein;
                     p53-binding protein Mdm2; murine double minute 2 homolog;
                     transformed 3T3 cell double minute 2 homolog; RING-type E3
                     ubiquitin transferase Mdm2; etID309863.4; MDM2 oncogene,
                     E3 ubiquitin protein ligase"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase Mdm2 isoform 2"
                     /protein_id="NP_571439.3"
                     /db_xref="GeneID:30637"
                     /db_xref="ZFIN:ZDB-GENE-990415-153"
                     /translation="

MATESCLSSSQISKVDNEKLVRPKVQLKSLLEDAGADKDVFTMKEVMFYLGKYIMSKELYDKQQQHIVHCGEDPLGAVLGVKSFSVKEPRALFALINRNLVTVKNPESQSTFSEPRSQSEPDRGPGDTDSDSRSSTSQQQRRRRRSSDPESSSAEDESRERRKRHKSDSFSLTFDDSLSWCVIGGLHRERGNSESSDANSNSDVGISRSEGSEESEDSDSDSDNFSVEFEVESINSDAYSENDVDSVPGENEIYEVTIFAEDEDSFDEDTEITEADYWKCPKCDQFNPPLPRHCKTCWTVRADWLPETHSNWENLSRNTRTNPEDTSVTTTPNTTFEKKLSKPSSPLPETDDGVDVPDGKCFPSPATTKDELPSSATITDSQTTSSQPSTSSGGGSSQEETPELERFNSLEACLPATCLEPCVICQSRPKNGCIVHGRTGHLMACYTCAKKLKNRNKLCPVCREPIQSVVLTYMS"

     misc_feature    102..350
                     /gene="mdm2"
                     /gene_synonym="sb:eu923; zgc:109834"
                     /note="p53-binding domain found in E3 ubiquitin-protein
                     ligase MDM2 and similar proteins; Region: MDM2; cd17672"
                     /db_xref="CDD:349491"
     misc_feature    order(189..191,198..203,210..215,228..230,243..245,
                     306..311,315..317,324..329)
                     /gene="mdm2"
                     /gene_synonym="sb:eu923; zgc:109834"
                     /note="p53 binding site [polypeptide binding]; other site"
                     /db_xref="CDD:349491"
     misc_feature    873..953
                     /gene="mdm2"
                     /gene_synonym="sb:eu923; zgc:109834"
                     /note="Zn-finger in Ran binding protein and others;
                     Region: zf-RanBP; pfam00641"
                     /db_xref="CDD:395516"
     misc_feature    1305..1475
                     /gene="mdm2"
                     /gene_synonym="sb:eu923; zgc:109834"
                     /note="Modified RING finger, HC subclass (C2H2C4-type),
                     found in E3 ubiquitin-protein ligase MDM2 and similar
                     proteins; Region: mRING-HC-C2H2C4_MDM2; cd16783"
                     /db_xref="CDD:438438"
     misc_feature    order(1347..1349,1353..1355,1359..1379,1431..1433,
                     1452..1454,1458..1463)
                     /gene="mdm2"
                     /gene_synonym="sb:eu923; zgc:109834"
                     /note="dimer interface [polypeptide binding]; other site"
                     /db_xref="CDD:438438"
     exon            111..185
                     /gene="mdm2"
                     /gene_synonym="sb:eu923; zgc:109834"
                     /inference="alignment:Splign:2.1.0"
     exon            186..319
                     /gene="mdm2"
                     /gene_synonym="sb:eu923; zgc:109834"
                     /inference="alignment:Splign:2.1.0"
     exon            320..369
                     /gene="mdm2"
                     /gene_synonym="sb:eu923; zgc:109834"
                     /inference="alignment:Splign:2.1.0"
     exon            370..428
                     /gene="mdm2"
                     /gene_synonym="sb:eu923; zgc:109834"
                     /inference="alignment:Splign:2.1.0"
     exon            429..498
                     /gene="mdm2"
                     /gene_synonym="sb:eu923; zgc:109834"
                     /inference="alignment:Splign:2.1.0"
     exon            499..656
                     /gene="mdm2"
                     /gene_synonym="sb:eu923; zgc:109834"
                     /inference="alignment:Splign:2.1.0"
     exon            657..806
                     /gene="mdm2"
                     /gene_synonym="sb:eu923; zgc:109834"
                     /inference="alignment:Splign:2.1.0"
     exon            807..875
                     /gene="mdm2"
                     /gene_synonym="sb:eu923; zgc:109834"
                     /inference="alignment:Splign:2.1.0"
     exon            876..2780
                     /gene="mdm2"
                     /gene_synonym="sb:eu923; zgc:109834"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

gggagcaaacggtcgattcgcgaaacggtcaccagcagataactaccaaaatggcaacagagagttgtttaagcagttctcagatcagcaaggttgacaacgagaaactggtaagaccaaaggtgcaattgaaaagcctgttagaggatgcaggtgcagataaagatgttttcaccatgaaggaggttatgttttatttagggaaatatatcatgagcaaggagttatatgacaagcagcagcaacacatagttcactgtggagaggatcctctcggtgctgttcttggagtgaaaagtttttcagttaaagagccgcgtgccctctttgcattgatcaacagaaaccttgtcacagtgaaaaatccagaatcacagtctaccttctctgaacccaggagtcaaagtgaaccagatcgaggacccggggatacagattcagactctcgctcatctacctcacaacagcagcgcaggaggaggagaagcagtgatcctgagagttcttcagctgaagacgagtctagagaacgcaggaagaggcataagtcggacagcttttccctgacatttgatgacagcctgtcttggtgcgtgattggtggcctgcaccgggagagggggaacagcgagtcttcggatgcaaacagcaactcggatgtaggtatatctcgcagtgagggcagtgaggagagcgaagactcagactctgattctgataactttagtgtggagtttgaggtggagtccatcaactctgatgcttacagtgagaatgatgtggattctgtcccaggagagaacgagatatatgaagtcacaatcttcgcagaagacgaagactcttttgatgaagatactgagataaccgaggcagactactggaagtgtcccaaatgtgaccagttcaaccctcctcttcctcgacactgcaaaacctgctggaccgtccgagctgattggcttccagaaacacactccaactgggaaaacctttcaagaaatactcgaaccaaccccgaagacaccagcgtcaccacaactcccaacacaaccttcgaaaagaaactctccaaaccctcaagccctctcccagaaacagatgatggagtggacgtacccgacggcaagtgctttccatctcctgccactaccaaagatgagcttccctcctctgctaccattactgactctcagaccacatcatcacagccctccacttcctccggcgggggcagcagtcaggaagagacccccgagctggagcgcttcaacagtctggaggcctgccttcccgctacgtgcctagaaccctgcgtcatctgtcagagtcgccccaagaacggctgcattgttcacggaaggactgggcaccttatggcgtgctacacatgtgccaaaaaattaaaaaaccggaacaagttgtgtccggtgtgccgagagcccattcagtcagtggtgttgacttacatgagctgaggcactgaaatatacctcctcctgtccttaaatgaagtttgggagttgccttgtggttgtattagatataaacgtttgttttcaaattcaatatttgtaatgattcaatatttttttcttattgaatacctaattttatttatgactcgtcgatttcactcacacgttttgtaataaattcacacacttatattttaaggattgtgttagaattgaaacaaaagcttgtgaggtgctgtattgattaaatagtctaatagaaatgtttgtaatttaatttatgtacatgcagatgtagaggtttttgagttgtttgctgcactgaaaaagagttccccgagctcatttaaaaatgttcttatgatatttcggctgcgaagactgtcttattttctttcagaaagacataataaatgattattttgaggttcttctgcttttacagatgagatcaaatctgctttttctgtcctctgctttgaatgattttctgatggcttgtatgtaggttagatgactggtggcagtagggggagttgtttttgttgggggaaatacacagcggtcacccagagagagcttcagttactagggaccctcttttcactctaatatcaagaaacatgttctgacttgtttctctctaatggcaacctagtacaaacactggccttttcaaatatctttttcacaaaaggtgtttttttttttcagatgaaattgtcagcatctatgacagttgtgttatgcaattatcattacaccacctatttaactgctaatttaattttagcaaacaagaaaaaaaaaacacttattggaagatttgatttttatttatttttaaaaataaactttttttttgtaataaactggttttgtagtgtttgcagcagcatggcaaatgaatctgagtctataagttcacctttttatttgtcagatggtgcacaaatgaagtgcgattagaacatgattgctggaatcagatgttttcatacacgaggtatgtatgcactttcctgcacttacatatgtatttgcacagtatgacagactgcaaacgaatgaacactttgtaaacaaattcacagatgttttaaaaggattaaatgcattcaagcttcagaaaatggcatcaatagcacaagtctgatctcttaaatgctatttatacgactatatacctctactttcatattgcaatggcatgtcctatagccatgtatttttatgtatttcttttttgtctcaaatgttatccaagtatttgaagaatatcaaataaatgcatttgttgac

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