LOCUS       NM_001362619            7214 bp    mRNA    linear   VRT 23-JUN-2025
DEFINITION  Danio rerio G protein-coupled receptor kinase 4 (grk4), transcript
            variant 1, mRNA.
ACCESSION   NM_001362619 XM_009306441 XM_073946444
VERSION     NM_001362619.2
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Gerhards,J., Maerz,L.D., Matthees,E.S.F., Donow,C., Moepps,B.,
            Premont,R.T., Burkhalter,M.D., Hoffmann,C. and Philipp,M.
  TITLE     Kinase Activity Is Not Required for G Protein-Coupled Receptor
            Kinase 4 Restraining mTOR Signaling during Cilia and Kidney
            Development
  JOURNAL   J Am Soc Nephrol 34 (4), 590-606 (2023)
   PUBMED   36810260
REFERENCE   2  (bases 1 to 7214)
  AUTHORS   Guo,H., Du,X., Zhang,Y., Wu,J., Wang,C., Li,M., Hua,X., Zhang,X.A.
            and Yan,J.
  TITLE     Specific miRNA-G Protein-Coupled Receptor Networks Regulate
            Sox9a/Sox9b Activities to Promote Gonadal Rejuvenation in Zebrafish
  JOURNAL   Stem Cells 37 (9), 1189-1199 (2019)
   PUBMED   31116894
REFERENCE   3  (bases 1 to 7214)
  AUTHORS   Bayes,A., Collins,M.O., Reig-Viader,R., Gou,G., Goulding,D.,
            Izquierdo,A., Choudhary,J.S., Emes,R.D. and Grant,S.G.
  TITLE     Evolution of complexity in the zebrafish synapse proteome
  JOURNAL   Nat Commun 8, 14613 (2017)
   PUBMED   28252024
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7214)
  AUTHORS   Pasquier,J., Cabau,C., Nguyen,T., Jouanno,E., Severac,D.,
            Braasch,I., Journot,L., Pontarotti,P., Klopp,C., Postlethwait,J.H.,
            Guiguen,Y. and Bobe,J.
  TITLE     Gene evolution and gene expression after whole genome duplication
            in fish: the PhyloFish database
  JOURNAL   BMC Genomics 17, 368 (2016)
   PUBMED   27189481
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7214)
  AUTHORS   Zhao,Z., Lee,R.T., Pusapati,G.V., Iyu,A., Rohatgi,R. and
            Ingham,P.W.
  TITLE     An essential role for Grk2 in Hedgehog signalling downstream of
            Smoothened
  JOURNAL   EMBO Rep 17 (5), 739-752 (2016)
   PUBMED   27113758
REFERENCE   6  (bases 1 to 7214)
  AUTHORS   Elkon,R., Milon,B., Morrison,L., Shah,M., Vijayakumar,S.,
            Racherla,M., Leitch,C.C., Silipino,L., Hadi,S., Weiss-Gayet,M.,
            Barras,E., Schmid,C.D., Ait-Lounis,A., Barnes,A., Song,Y.,
            Eisenman,D.J., Eliyahu,E., Frolenkov,G.I., Strome,S.E., Durand,B.,
            Zaghloul,N.A., Jones,S.M., Reith,W. and Hertzano,R.
  TITLE     RFX transcription factors are essential for hearing in mice
  JOURNAL   Nat Commun 6, 8549 (2015)
   PUBMED   26469318
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 7214)
  AUTHORS   Valanne,S., Myllymaki,H., Kallio,J., Schmid,M.R., Kleino,A.,
            Murumagi,A., Airaksinen,L., Kotipelto,T., Kaustio,M., Ulvila,J.,
            Esfahani,S.S., Engstrom,Y., Silvennoinen,O., Hultmark,D.,
            Parikka,M. and Ramet,M.
  TITLE     Genome-wide RNA interference in Drosophila cells identifies G
            protein-coupled receptor kinase 2 as a conserved regulator of
            NF-kappaB signaling
  JOURNAL   J Immunol 184 (11), 6188-6198 (2010)
   PUBMED   20421637
REFERENCE   8  (bases 1 to 7214)
  AUTHORS   Miranda,C.L., Collodi,P., Zhao,X., Barnes,D.W. and Buhler,D.R.
  TITLE     Regulation of cytochrome P450 expression in a novel liver cell line
            from zebrafish (Brachydanio rerio)
  JOURNAL   Arch Biochem Biophys 305 (2), 320-327 (1993)
   PUBMED   8373170
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JBMGRA010000001.1.
            
            On or before Jun 23, 2025 this sequence version replaced
            XM_073946444.1, NM_001362619.1.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: GFIL01011992.1,
                                           SRR32588727.1912829.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA3505370,
                                           SAMEA3505371 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-355               JBMGRA010000001.1  13372941-13373295   c
            356-451             JBMGRA010000001.1  13356530-13356625   c
            452-564             JBMGRA010000001.1  13349399-13349511   c
            565-642             JBMGRA010000001.1  13348854-13348931   c
            643-743             JBMGRA010000001.1  13348599-13348699   c
            744-836             JBMGRA010000001.1  13343671-13343763   c
            837-900             JBMGRA010000001.1  13338630-13338693   c
            901-1041            JBMGRA010000001.1  13337653-13337793   c
            1042-1232           JBMGRA010000001.1  13327984-13328174   c
            1233-1270           JBMGRA010000001.1  13323840-13323877   c
            1271-1360           JBMGRA010000001.1  13320264-13320353   c
            1361-1569           JBMGRA010000001.1  13319970-13320178   c
            1570-1707           JBMGRA010000001.1  13317464-13317601   c
            1708-1845           JBMGRA010000001.1  13317222-13317359   c
            1846-1977           JBMGRA010000001.1  13316177-13316308   c
            1978-7214           JBMGRA010000001.1  13307018-13312254   c
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /strain="Tuebingen"
                     /db_xref="taxon:7955"
                     /chromosome="1"
                     /map="1"
     gene            1..7214
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /note="G protein-coupled receptor kinase 4"
                     /db_xref="GeneID:560761"
                     /db_xref="ZFIN:ZDB-GENE-060929-1198"
     exon            1..355
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    265..267
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /note="upstream in-frame stop codon"
     CDS             304..2043
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /EC_number="2.7.1.-"
                     /note="isoform 1 is encoded by transcript variant 1; GRK5"
                     /codon_start=1
                     /product="G protein-coupled receptor kinase 4 isoform 1"
                     /protein_id="NP_001349548.1"
                     /db_xref="GeneID:560761"
                     /db_xref="ZFIN:ZDB-GENE-060929-1198"
                     /translation="

MEIENIVANTVLLKAREGGGGKRNGRSKKWKEMLKLPHISQCEELRRTIERDYTSLCEKQPIGRQLFRQFCDTQPKLRRCIEFLDSVAMYQVAPDEKRRDAGLNILDTYFNNGSAAHLPEMAQDVVGECRQKLEQSPCKELFQECTRIVREYLSGAPFSAYQETMYFSRFLQWKWLERQPVTKNTFRHYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRVKKRKGEAMALNEKRILEKVNSSFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNSGFDEQRAIFYAAEICCGLEDLHRERIVYRDLKPENILLDDRGHIRISDLGLAVQIPEGETIRGRVGTVGYMAPEVIQNESYTFSPDWWGLGCLIYEMIQGQSPFRRRKERVKREEVDRRVCEDQEEYTEKFSEDAKDICRQLLCKDPKERLGCRGSGAAEVKLHPIFRNINFKRLEANMLDPPFCPDPRAVYCKDVLDIEQFSTVKGVNLDPTDDDFYHKFVTGSVSIPWQNEMIEMECFKEINVFEMDGALCADLDVNRPNPAPKRGFFYRLFHREVCFGSANSEEEEKEEKEEEPS"

     misc_feature    457..852
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /note="Regulator of G protein signaling domain (RGS) found
                     in G protein-coupled receptor kinase 4 (GRK4); Region:
                     RGS_GRK4; cd08750"
                     /db_xref="CDD:188704"
     misc_feature    order(508..510,517..519,790..792,796..801,805..810)
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /note="putative dimer interface [polypeptide binding];
                     other site"
                     /db_xref="CDD:188704"
     misc_feature    856..1710
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; Region: Protein
                     Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     misc_feature    order(877..888,895..897,901..903,940..942,946..948,
                     1042..1044,1090..1101,1234..1236,1246..1251,1255..1257,
                     1285..1290)
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /note="ATP binding site [chemical binding]; other site"
                     /db_xref="CDD:270870"
     exon            356..451
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            452..564
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            565..642
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            643..743
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            744..836
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            837..900
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            901..1041
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1232
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1270
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            1271..1360
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            1361..1569
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            1570..1707
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            1708..1845
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            1846..1977
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
     exon            1978..7214
                     /gene="grk4"
                     /gene_synonym="zgc:153020"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

gtaagcgagagctcgagacaggaaggactgctgagcactgaatgctaacaagggggagagagagtacatgtccacggcgagcggaaaggtggggggttcaacagcgagatatggagaaatacaattaacctgcaatgtgccaacgccaaactcacgccgatcataaccatcctcaccaccgatcatcatcatcatcatcctcaccctcatcaccaccaccagcagcaacggcgcgaccgacctcgtcttttttctcctaagaagtgaaatgtatcgggactgcatgggatctttcttctgacaatggagattgagaatatcgtggccaacaccgtgctgctgaaggcgagggaaggaggcggagggaagcgtaatggacggagcaagaaatggaaggagatgctgaaattgcctcatatcagtcagtgtgaggaactgagacgcacaattgagagggattatacaagcctttgtgaaaagcagccaattgggcgtcagttgtttcgtcaattctgtgacactcagcccaaattacggcgctgtatagagttcctggattctgtggcaatgtatcaggtggctccggatgagaagaggagagacgccggtttaaatattttagacacatattttaataacgggtcagccgctcatctaccagagatggctcaggatgtggtcggggaatgcaggcagaaactggaacagagtccatgtaaagagctgttccaggagtgtaccaggatagttcgtgaatatttaagtggagcgccattctcggcttatcaggagactatgtacttttcccgttttcttcagtggaagtggttggagaggcaaccagtaaccaaaaacacattcagacattacagggtattgggcaagggcggatttggtgaggtttgtgcctgccaggttcgtgccactggtaagatgtacgcctgtaaaaagctggagaagaaacgtgtgaagaaaagaaaaggtgaagcaatggcattaaatgaaaaacgcattttagaaaaagttaacagtagttttgtagttagtttggcatatgcgtatgagactaaggacgctctatgtttggtgttgaccataatgaacggtggtgatctgaagtttcatatctataacatgggaaactcaggtttcgatgagcagcgggccatcttttacgcggcagagatctgttgcggattagaggatctgcatcgagagcggatcgtctacagagacttgaaaccagaaaatattctgctagatgatcgtgggcacatccgtatttctgatctgggactggccgtgcaaattccagagggcgagacgatacgagggcgtgttggcacagtggggtacatggcaccagaggtcatccagaacgagagctacaccttcagtcctgactggtggggtttgggctgtctaatttacgaaatgattcagggtcagtctcctttccgtcgccgtaaagagcgagtgaagagagaggaagtggaccggcgagtttgtgaggaccaggaggaatatactgagaagttctcagaagatgctaaagacatctgccgtcagttgttatgtaaagatccaaaggagcgcttgggctgtcggggcagtggagcggccgaagtcaaactgcatcccattttccgcaacatcaacttcaagcggctggaggccaacatgctggacccgccgttctgtccagacccgcgagcagtttactgtaaggacgtcctggacatagagcagttttcgacagtcaaaggtgtaaacctggacccaacagatgacgatttctaccacaagtttgtaacagggagtgtctccatcccatggcaaaatgagatgattgagatggagtgttttaaggagatcaacgtgttcgagatggacggagcgctgtgtgctgatctggatgtgaacagacccaatccagcaccgaagcgaggattcttctaccgtttatttcaccgagaggtttgttttgggagtgccaacagtgaggaggaggagaaagaggagaaggaggaggagccatcttgacttaaaaccactcccacttcccatcagctgccaatcagaatgctaaacgtatgaatgttcaactgtatttatgaactgtatcaaactgtatcataattaaagaaaagggtatgagtttaaagaattttgtacatttgtacttgaattatgtgtagatgtatattttcactaaaggttgtattgtacaaaaagccatagaaataccacttgaatgcatatataatgtttttattgttattatttttcatctgtactgaatatgaaatgtgtatttctttttaagcacaatataaacactcaccttttgtttccaatgctaacgctctgttagcactgcctttgtttgtatgttaacttagatcgtttggctggacagtaaaccgtgcttcttttgtacagcagggtaagatcaatcacacataaagagggagcagtacaaatgatctattcatatcatatcatttacagtggtcaaccagaatgctaatatcaaaagcaggggtggctgtggtcaaataaaaacaaaaaaacaaactgtatatagcctagatatataaaaacgaggttcaaaagtctgagaataccaagtaaaatgcttcaaaatatatattcagaaaaatctggactgaaaatgaattgcgtgcaataataacacctttgttaagcaatatgtggctaattttgagattcaaactgttaggaaatatactcataatttcaaaattagcctaatgttgatgtattaaaacatattaatgctttattgccaccttcactcattcccccccaatttttgtcaaatttgacatgcctttttcttcaacccaggctcattctgaaaatgtacccccgtatacatttctggagagcactaattacatccctggagctacgtttttgcttttttttttcgaattccccagaggccgctgtgtatgctttttaaaatctaaattttttctagcaagtaccatttaaacctgctgttcttgcgtaaatccaccagaggctgctgtcaactgactgactgaccgactgacccaccctcctccttccctaaacccaaccaatagtgtttccaatagcaccgattgacccgccttcgctaaccacaaccgacagtttaaaaagcaatccaaaaaaagaaaagcccttgcctgatttttaccatgttttcagatttttccacattctcaccgttatttacttcattttattttttggcttctgtttttgtcttacccgctttctggaaccattcgaaccctgtcgttggggtcaactcctctctgcgtctcaagtcatacatgactcgacatacatggcgagctgctggacaaactgataacagtagaaacgctgtttacatggaagtcagcggtcagctggaaagcgagaaaagaccaccctgtagccttcattttaaagacgaaatgctgccatatgtacctctggctacataattcatgatcttcttaaatgtatatagggctatgtttttagagtgagcctatgttgttgttgttgttttgtaaatgtttatttttagggctaaattgaatatataactccagattttcagtgtgtcctcagacttctgaaccccacctgtgtgttgacctcattgacaagctgtctggattttgtacaaaaggaaagtgttcatacatatgttaatgataatgggtgtgccaatagctagtgccaatagcctcaaaacaaccagatgattaataggtccaatccaatgactaataacaatgtgtaacttgcgggttgtggaagtgtaacgtcattcattttctccatagggaatttttttttttaaactgataactgcagtcttgttgtgagctctgaggttcttgattgtatatgctgttgctgaacttgtctattcacattaaatcactttattatttcaacagtcatttttgattgtagaatttggccataacaacgacattacccatgatgctgtgcagaaaaccaaccaatcagacagttgcagcgtttctgattgtagaatctggcaaaagcaaccaatttacccatgatactgtgcagaaaactcaccaatcagactgttgcagtgtttttgatcatagaattgggccataacaaccacattaaacattatgctgtgcagaaaacccaccaatcagagagttgtagcattttacattttagaatttggccataacaacaacattacccatgatgctatgcagaaaacccaccaatcagacagttgcagctgacaaagcaactttacattatatcactttattatagtcaattttgatcgtagaattgggccataacaacaacattacccgtgatgctgtgcagaaaagccaccaatcagacagttgcagcttttttgattgtagaattggtccataacaactacattacccatgatgctgtgcattaaagccaccaatcagacagttgcagcgattttaattgtagaatttcgccataactacaatacccatgatgctgtgcagaaaacctatcaatcagaccgttgcagcgtttttgattgtagaatttggccaaaacaactacattacccatgatgctgtgcagaaaagttaccaatcggacagttgctgctaacaaagcaacttcagattaaatcaccttttttattttaacgatcatttttgattgtagaatctggccataacaactacattacccaacatgctgtgtgcataaaacccaccaatcagacagttgcagcattttttattatagaatttggctataacaactacattacccatgatgctgtgcacaaaacccaccaatcaggcacttacagcatttttgatagtagaatttggccataacaactacattacccatgatgctgtgcagaaaatccatcaatcagacagttgctgcatttttgattgtagaatttggccataacagccacattacccatgatgctttgcagaaaacccaccaatcagacagttgtagcgttttaatggtagaattgggacacaacaaccacattacccatgatgctgtgcagaaaacccaccaattagacagttgcagctaacatagcacgtcaagagatctttaactccaaaaacttccatgaagcaccccaaaatattttccaaaaaagcttaaaatatggataaaacaaacacctttaattgaacaattttttatttcttaatcgttttaatctgattatgttttgtgcaatgctttatgggattgtagttctttttctcatttaacctgttcagtatgtcttgtacttcttctcagatttgtaaggttgtgattcacctcagatgattagacagttttattactcaaaaaaataaatattttttaatccctatggtaaaatgaacaggcaaatactttcagaaaaagtgaacgtcagcactagtcgtaaaattgttctttcattaaattcatgcagctttaacgaaatgcatgaaaccaataatctctgtgcatcgggtgactaatcccagttttggttacgcattaacaagacacatatgtatgttaattaacgtagtcaccgtagctttcagatgcatcaacgacagggaaccaggcatgttgttcaacgatagactaatatgagttttttttttcagtggcagataatcgatatctagtacctagtaatatggacatgtacgtaaataagtgactgaatgatggcatattacactatatttactcaaaacccacatacgattgaacatataaaatgtttactatccattaacatggctgttgttcggtttttggagttgatacaaggaaatgttaattactctagctatgtgtcagtagttttcaggccgctaatctgaaatgatttattttttccgttttgttgactgtttgctaatatccactctcatggttacatttagtgccatcaggaaactttaaaccaacgaaataagcttcatctttgaatttgctcttgaacgagctgtcgattttgccttaggtgtcatgtgttcggtcgacattccaaacggagtatcgatcgatcccttaacgataatcatatgatgtaattaatgactgtgcagtttgttcgttaaacacttgcacattttacattttgctttgtatgtatttgtgtggcatattttaatcgcaatgaaggcttccatttagtgatgggaattagcatctcgatcttacgagaaactattttacatgtcatcagaaaagtggctaacttgtacaaattcgtacggtttcatttgcatgaaaacataagatttttttaaaagtaggagtggccccaaaccccacccaaaacttattcctcattgggtggaattagtgaatcgtattaaaatgtacatatgagatcgtacaaattaatatgaattagccacttcatcaaaaagttacgaattgagaattgtgttggaattagtctcaaatgtattgttcataaattgggtaatattacatttgatttaaatggaggaagtgaaaattagttttgcttccattcagtggtgggaattagcagctcgatctcacgaggaactattttacatgtcagaaaagaggctaattcgtataaatgcatacaatttaatttgtatgaaaacgtacaatttttaaaaagtgggtgtggccccaaaccccacccctaaccctacccgtcattgggtggaattatcgaatcttattaaaatttacgaattagatcgtacaattaatacaaattagccactacatcaaaaagttacgaatttagaattgcgttggtattcaaatgtcaaatgtatcattcggaaattgcataatattacatttgaattaaatgaaggaagtggaaattagttttgatctaaaaaatgcttttgtctaacgcaccttcgaaatcagagtcccaaccaatggcgtgagtcgggggcggggctataacatgcttcagcactgacagtaaagctcaaataggaaccattattttacctccatcacccatttttgtctttatgaggatctttatggtcccgtttgcaaagctgtgaactgaaaacaactcctcttctgttattcccatatgtgtgcatgttctactgtgtccatggttttacaagaggatgagcactcactttagttgacaataaagttatgagttgaca

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