LOCUS       NM_001080025            1647 bp    mRNA    linear   VRT 05-JAN-2026
DEFINITION  Danio rerio POU class 2 homeobox associating factor 2b (pou2af2b),
            mRNA.
ACCESSION   NM_001080025 XM_689358
VERSION     NM_001080025.2
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Gaudet,P., Livstone,M.S., Lewis,S.E. and Thomas,P.D.
  TITLE     Phylogenetic-based propagation of functional annotations within the
            Gene Ontology consortium
  JOURNAL   Brief Bioinform 12 (5), 449-462 (2011)
   PUBMED   21873635
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Lo,J., Lee,S., Xu,M., Liu,F., Ruan,H., Eun,A., He,Y., Ma,W.,
            Wang,W., Wen,Z. and Peng,J.
  TITLE     15000 unique zebrafish EST clusters and their future use in
            microarray for profiling gene expression patterns during
            embryogenesis
  JOURNAL   Genome Res 13 (3), 455-466 (2003)
   PUBMED   12618376
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JBMGRA010000005.1.
            
            On Jun 27, 2024 this sequence version replaced NM_001080025.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC127593.1, GDQH01021875.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA3505387, SAMEA4476753
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-384               JBMGRA010000005.1  31001650-31002033
            385-485             JBMGRA010000005.1  31004299-31004399
            486-528             JBMGRA010000005.1  31005746-31005788
            529-833             JBMGRA010000005.1  31005901-31006205
            834-1647            JBMGRA010000005.1  31008329-31009142
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /strain="Tuebingen"
                     /db_xref="taxon:7955"
                     /chromosome="5"
                     /map="5"
     gene            1..1647
                     /gene="pou2af2b"
                     /gene_synonym="pou2af2; si:dkey-180i4.1; zgc:158412"
                     /note="POU class 2 homeobox associating factor 2b"
                     /db_xref="GeneID:566090"
                     /db_xref="ZFIN:ZDB-GENE-061201-18"
     exon            1..384
                     /gene="pou2af2b"
                     /gene_synonym="pou2af2; si:dkey-180i4.1; zgc:158412"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    351..353
                     /gene="pou2af2b"
                     /gene_synonym="pou2af2; si:dkey-180i4.1; zgc:158412"
                     /note="upstream in-frame stop codon"
     CDS             372..1250
                     /gene="pou2af2b"
                     /gene_synonym="pou2af2; si:dkey-180i4.1; zgc:158412"
                     /note="Uncharacterized protein C11orf53 homolog; protein
                     POU2AF2"
                     /codon_start=1
                     /product="POU class 2 homeobox associating-factor 2"
                     /protein_id="NP_001073494.2"
                     /db_xref="GeneID:566090"
                     /db_xref="ZFIN:ZDB-GENE-061201-18"
                     /translation="

MMETEYSKRVYQGVRVKHTVKDLLAEKRLRQTNTPRFSTSSSSSQPAFVPMPGSHVLPGYYSMRRSFLQDSELCHTVKQYSSDTYSSALGSKAFSYDHASTYPAFIDSYYTPDSYGDYRGPTSYTTSGGSLFPPSSLPTLLPTLSGESSSHLLLRDPWDQPSEDSVNQTEVLCPEAAAPVADSPSLAGPDSGSSSPYRLTSGRSGSSIPSSSQPYTLQPLEDVPYTAPSYTSASSYSCPPYMSTPGDLAVVKMTAVTSEEASGGVVSLSDTTSWAKDDGTGSWLSYETRRAF"

     misc_feature    528..1247
                     /gene="pou2af2b"
                     /gene_synonym="pou2af2; si:dkey-180i4.1; zgc:158412"
                     /note="Family of unknown function (DUF5566); Region:
                     DUF5566; pfam17721"
                     /db_xref="CDD:465469"
     exon            385..485
                     /gene="pou2af2b"
                     /gene_synonym="pou2af2; si:dkey-180i4.1; zgc:158412"
                     /inference="alignment:Splign:2.1.0"
     exon            486..528
                     /gene="pou2af2b"
                     /gene_synonym="pou2af2; si:dkey-180i4.1; zgc:158412"
                     /inference="alignment:Splign:2.1.0"
     exon            529..833
                     /gene="pou2af2b"
                     /gene_synonym="pou2af2; si:dkey-180i4.1; zgc:158412"
                     /inference="alignment:Splign:2.1.0"
     exon            834..1647
                     /gene="pou2af2b"
                     /gene_synonym="pou2af2; si:dkey-180i4.1; zgc:158412"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

ctttcctcgtctttatctaatgatattcctcttttttcttcctcatttcctcgtaatcaacttttaatacgactgtcacactccccattttcagcaggtttaatagtgtactctgatctacagtagatgcaaattattaagaaacgtctccaagccagttatttgcatttaaccaaatcatgaaggcttcacaagcgaaaccatgtgaaggatatgaatagaggtgcgtgagcttgaccatataggctacatgtctagtaggttttaaaatctccctttcttcctttgatataatgaccgtgttatgagttctcctggaatagaaaacaaaacggaagaactgagagacctgacagcgatcggctaaaattatgatggagacagagtattccaagagagtataccagggagtccgtgtcaagcatacggtcaaagatctactggccgagaagcgattacgacaaacaaacacaccccgattcagtacaagcagcagttcctctcagccagcttttgtgccaatgcccggttcgcatgtgcttcctggttactacagtatgagaagatccttcctccaagactcggagctctgccatactgtgaagcagtactcctcggacacgtactcttcggcactgggaagcaaggccttctcatatgaccacgcttccacttatcctgccttcatcgacagctactacactcctgattcgtatggagattacagagggccaacatcttataccacaagcggagggtcattgtttccaccgtcttcactaccaacactactgccgactctgtctggagagtcttcatcacacttgctcttgagagacccatgggatcagccatcagaggactcggtcaatcagacagaagtcttatgtcctgaggctgcagcccccgtggcagactcaccatccctagctgggcctgactcaggcagttcctctccgtaccgtctgacctctggtcggagtgggagctccatcccatccagctctcagccatatactctacagcctctggaggacgtcccatacacagcaccatcttacacctctgcctccagctactcctgcccaccgtacatgtcgactcctggagatctggctgtggtgaagatgacagccgtcacttcagaggaggccagtggtggagtggtgtctctcagtgacactacatcctgggctaaagatgatggaacaggatcctggttgtcatatgagaccagaagagctttttaagaggcctcaatgacaattacaggactgtggactaatcattttgcaattatgcagcctctctattcttacacaaaaggagattatatagattcatcataacgatgaaatgctggtcataaaaaactgcataccacatatagatacagtaaaacatgtgctgttagtctagttaattcatggtttaattgtgcattttttaatttccaagcgttttagaataaattagaggtaatgctccatttccccctctttcttacaattccaagtgtaatattactcccaaaaacgtacattttgactggaaacataacataaaccaacatttttctgctcaacaaaaatggtagctctttgaaaaaaaacgtgaatttgaaaaataaatatgttttggtttgaa

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