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Previous release (v1)
2025-09-13 16:53:59, GGRNA.v2 : RefSeq release 231 (Jul, 2025)
LOCUS NM_001079986 7263 bp mRNA linear VRT 23-JUN-2025
DEFINITION Danio rerio G protein-coupled receptor kinase 4 (grk4), transcript
variant 2, mRNA.
ACCESSION NM_001079986 XM_009306444 XM_073946452 XM_684159
VERSION NM_001079986.3
KEYWORDS RefSeq.
SOURCE Danio rerio (zebrafish)
ORGANISM Danio rerio
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE 1 (bases 1 to 7263)
AUTHORS Gerhards,J., Maerz,L.D., Matthees,E.S.F., Donow,C., Moepps,B.,
Premont,R.T., Burkhalter,M.D., Hoffmann,C. and Philipp,M.
TITLE Kinase Activity Is Not Required for G Protein-Coupled Receptor
Kinase 4 Restraining mTOR Signaling during Cilia and Kidney
Development
JOURNAL J Am Soc Nephrol 34 (4), 590-606 (2023)
PUBMED 36810260
REFERENCE 2 (bases 1 to 7263)
AUTHORS Guo,H., Du,X., Zhang,Y., Wu,J., Wang,C., Li,M., Hua,X., Zhang,X.A.
and Yan,J.
TITLE Specific miRNA-G Protein-Coupled Receptor Networks Regulate
Sox9a/Sox9b Activities to Promote Gonadal Rejuvenation in Zebrafish
JOURNAL Stem Cells 37 (9), 1189-1199 (2019)
PUBMED 31116894
REFERENCE 3 (bases 1 to 7263)
AUTHORS Bayes,A., Collins,M.O., Reig-Viader,R., Gou,G., Goulding,D.,
Izquierdo,A., Choudhary,J.S., Emes,R.D. and Grant,S.G.
TITLE Evolution of complexity in the zebrafish synapse proteome
JOURNAL Nat Commun 8, 14613 (2017)
PUBMED 28252024
REMARK Publication Status: Online-Only
REFERENCE 4 (bases 1 to 7263)
AUTHORS Pasquier,J., Cabau,C., Nguyen,T., Jouanno,E., Severac,D.,
Braasch,I., Journot,L., Pontarotti,P., Klopp,C., Postlethwait,J.H.,
Guiguen,Y. and Bobe,J.
TITLE Gene evolution and gene expression after whole genome duplication
in fish: the PhyloFish database
JOURNAL BMC Genomics 17, 368 (2016)
PUBMED 27189481
REMARK Publication Status: Online-Only
REFERENCE 5 (bases 1 to 7263)
AUTHORS Zhao,Z., Lee,R.T., Pusapati,G.V., Iyu,A., Rohatgi,R. and
Ingham,P.W.
TITLE An essential role for Grk2 in Hedgehog signalling downstream of
Smoothened
JOURNAL EMBO Rep 17 (5), 739-752 (2016)
PUBMED 27113758
REFERENCE 6 (bases 1 to 7263)
AUTHORS Elkon,R., Milon,B., Morrison,L., Shah,M., Vijayakumar,S.,
Racherla,M., Leitch,C.C., Silipino,L., Hadi,S., Weiss-Gayet,M.,
Barras,E., Schmid,C.D., Ait-Lounis,A., Barnes,A., Song,Y.,
Eisenman,D.J., Eliyahu,E., Frolenkov,G.I., Strome,S.E., Durand,B.,
Zaghloul,N.A., Jones,S.M., Reith,W. and Hertzano,R.
TITLE RFX transcription factors are essential for hearing in mice
JOURNAL Nat Commun 6, 8549 (2015)
PUBMED 26469318
REMARK Publication Status: Online-Only
REFERENCE 7 (bases 1 to 7263)
AUTHORS Valanne,S., Myllymaki,H., Kallio,J., Schmid,M.R., Kleino,A.,
Murumagi,A., Airaksinen,L., Kotipelto,T., Kaustio,M., Ulvila,J.,
Esfahani,S.S., Engstrom,Y., Silvennoinen,O., Hultmark,D.,
Parikka,M. and Ramet,M.
TITLE Genome-wide RNA interference in Drosophila cells identifies G
protein-coupled receptor kinase 2 as a conserved regulator of
NF-kappaB signaling
JOURNAL J Immunol 184 (11), 6188-6198 (2010)
PUBMED 20421637
REFERENCE 8 (bases 1 to 7263)
AUTHORS Miranda,C.L., Collodi,P., Zhao,X., Barnes,D.W. and Buhler,D.R.
TITLE Regulation of cytochrome P450 expression in a novel liver cell line
from zebrafish (Brachydanio rerio)
JOURNAL Arch Biochem Biophys 305 (2), 320-327 (1993)
PUBMED 8373170
COMMENT VALIDATED REFSEQ: This record has undergone validation or
preliminary review. The reference sequence was derived from
JBMGRA010000001.1.
On or before Jun 23, 2025 this sequence version replaced
XM_073946452.1, NM_001079986.2.
Transcript Variant: This variant (2) uses an alternate splice
junction in the 3' end compared to variant 1, that causes a
frameshift. The resulting isoform (2) has a shorter and distinct
C-terminus compared to isoform 1.
Sequence Note: The RefSeq transcript and protein were derived from
genomic sequence to make the sequence consistent with the reference
genome assembly. The genomic coordinates used for the transcript
record were based on alignments.
##Evidence-Data-START##
Transcript exon combination :: SRR32588727.1494754.1,
SRR32588724.2336196.1 [ECO:0000332]
RNAseq introns :: mixed sample support SAMEA3505370,
SAMEA3505372 [ECO:0006172]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP
1-355 JBMGRA010000001.1 13372941-13373295 c
356-451 JBMGRA010000001.1 13356530-13356625 c
452-564 JBMGRA010000001.1 13349399-13349511 c
565-642 JBMGRA010000001.1 13348854-13348931 c
643-743 JBMGRA010000001.1 13348599-13348699 c
744-836 JBMGRA010000001.1 13343671-13343763 c
837-900 JBMGRA010000001.1 13338630-13338693 c
901-1041 JBMGRA010000001.1 13337653-13337793 c
1042-1232 JBMGRA010000001.1 13327984-13328174 c
1233-1270 JBMGRA010000001.1 13323840-13323877 c
1271-1360 JBMGRA010000001.1 13320264-13320353 c
1361-1569 JBMGRA010000001.1 13319970-13320178 c
1570-1707 JBMGRA010000001.1 13317464-13317601 c
1708-1845 JBMGRA010000001.1 13317222-13317359 c
1846-2026 JBMGRA010000001.1 13316128-13316308 c
2027-7263 JBMGRA010000001.1 13307018-13312254 c
FEATURES Location/Qualifiers
source 1..7263
/organism="Danio rerio"
/mol_type="mRNA"
/strain="Tuebingen"
/db_xref="taxon:7955"
/chromosome="1"
/map="1"
gene 1..7263
/gene="grk4"
/gene_synonym="zgc:153020"
/note="G protein-coupled receptor kinase 4"
/db_xref="GeneID:560761"
/db_xref="ZFIN:ZDB-GENE-060929-1198"
exon 1..355
/gene="grk4"
/gene_synonym="zgc:153020"
/inference="alignment:Splign:2.1.0"
misc_feature 265..267
/gene="grk4"
/gene_synonym="zgc:153020"
/note="upstream in-frame stop codon"
CDS 304..2025
/gene="grk4"
/gene_synonym="zgc:153020"
/EC_number="2.7.1.-"
/note="isoform 2 is encoded by transcript variant 2; GRK5"
/codon_start=1
/product="G protein-coupled receptor kinase 4 isoform 2"
/protein_id="NP_001073455.1"
/db_xref="GeneID:560761"
/db_xref="ZFIN:ZDB-GENE-060929-1198"
/translation="
MEIENIVANTVLLKAREGGGGKRNGRSKKWKEMLKLPHISQCEELRRTIERDYTSLCEKQPIGRQLFRQFCDTQPKLRRCIEFLDSVAMYQVAPDEKRRDAGLNILDTYFNNGSAAHLPEMAQDVVGECRQKLEQSPCKELFQECTRIVREYLSGAPFSAYQETMYFSRFLQWKWLERQPVTKNTFRHYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRVKKRKGEAMALNEKRILEKVNSSFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNSGFDEQRAIFYAAEICCGLEDLHRERIVYRDLKPENILLDDRGHIRISDLGLAVQIPEGETIRGRVGTVGYMAPEVIQNESYTFSPDWWGLGCLIYEMIQGQSPFRRRKERVKREEVDRRVCEDQEEYTEKFSEDAKDICRQLLCKDPKERLGCRGSGAAEVKLHPIFRNINFKRLEANMLDPPFCPDPRAVYCKDVLDIEQFSTVKGVNLDPTDDDFYHKFVTGSVSIPWQNEMIEMECFKEINVFEMDGALCADLDVNRPNPAPKRGFFYRLFHREASGSASPAVLRGGGA"
misc_feature 457..852
/gene="grk4"
/gene_synonym="zgc:153020"
/note="Regulator of G protein signaling domain (RGS) found
in G protein-coupled receptor kinase 4 (GRK4); Region:
RGS_GRK4; cd08750"
/db_xref="CDD:188704"
misc_feature order(508..510,517..519,790..792,796..801,805..810)
/gene="grk4"
/gene_synonym="zgc:153020"
/note="putative dimer interface [polypeptide binding];
other site"
/db_xref="CDD:188704"
misc_feature 856..1710
/gene="grk4"
/gene_synonym="zgc:153020"
/note="Catalytic domain of the Serine/Threonine Kinase, G
protein-coupled Receptor Kinase 4; Region: STKc_GRK4;
cd05631"
/db_xref="CDD:173720"
misc_feature order(877..891,901..903,940..942,946..948,1042..1044,
1090..1095,1099..1101,1111..1113,1117..1119,1234..1236,
1240..1242,1246..1251,1255..1257,1285..1290,1297..1299,
1336..1353,1432..1434,1450..1452,1459..1461)
/gene="grk4"
/gene_synonym="zgc:153020"
/note="active site"
/db_xref="CDD:173720"
misc_feature order(877..891,901..903,940..942,946..948,1093..1101,
1255..1257,1288..1290)
/gene="grk4"
/gene_synonym="zgc:153020"
/note="ATP binding site [chemical binding]; other site"
/db_xref="CDD:173720"
misc_feature order(889..891,1111..1113,1117..1119,1234..1236,
1240..1242,1246..1248,1297..1299,1336..1353,1432..1434,
1450..1452,1459..1461)
/gene="grk4"
/gene_synonym="zgc:153020"
/note="polypeptide substrate binding site [polypeptide
binding]; other site"
/db_xref="CDD:173720"
misc_feature 1285..1353
/gene="grk4"
/gene_synonym="zgc:153020"
/note="activation loop (A-loop); other site"
/db_xref="CDD:173720"
exon 356..451
/gene="grk4"
/gene_synonym="zgc:153020"
/inference="alignment:Splign:2.1.0"
exon 452..564
/gene="grk4"
/gene_synonym="zgc:153020"
/inference="alignment:Splign:2.1.0"
exon 565..642
/gene="grk4"
/gene_synonym="zgc:153020"
/inference="alignment:Splign:2.1.0"
exon 643..743
/gene="grk4"
/gene_synonym="zgc:153020"
/inference="alignment:Splign:2.1.0"
exon 744..836
/gene="grk4"
/gene_synonym="zgc:153020"
/inference="alignment:Splign:2.1.0"
exon 837..900
/gene="grk4"
/gene_synonym="zgc:153020"
/inference="alignment:Splign:2.1.0"
exon 901..1041
/gene="grk4"
/gene_synonym="zgc:153020"
/inference="alignment:Splign:2.1.0"
exon 1042..1232
/gene="grk4"
/gene_synonym="zgc:153020"
/inference="alignment:Splign:2.1.0"
exon 1233..1270
/gene="grk4"
/gene_synonym="zgc:153020"
/inference="alignment:Splign:2.1.0"
exon 1271..1360
/gene="grk4"
/gene_synonym="zgc:153020"
/inference="alignment:Splign:2.1.0"
exon 1361..1569
/gene="grk4"
/gene_synonym="zgc:153020"
/inference="alignment:Splign:2.1.0"
exon 1570..1707
/gene="grk4"
/gene_synonym="zgc:153020"
/inference="alignment:Splign:2.1.0"
exon 1708..1845
/gene="grk4"
/gene_synonym="zgc:153020"
/inference="alignment:Splign:2.1.0"
exon 1846..2026
/gene="grk4"
/gene_synonym="zgc:153020"
/inference="alignment:Splign:2.1.0"
exon 2027..7263
/gene="grk4"
/gene_synonym="zgc:153020"
/inference="alignment:Splign:2.1.0"
ORIGIN
gtaagcgagagctcgagacaggaaggactgctgagcactgaatgctaacaagggggagagagagtacatgtccacggcgagcggaaaggtggggggttcaacagcgagatatggagaaatacaattaacctgcaatgtgccaacgccaaactcacgccgatcataaccatcctcaccaccgatcatcatcatcatcatcctcaccctcatcaccaccaccagcagcaacggcgcgaccgacctcgtcttttttctcctaagaagtgaaatgtatcgggactgcatgggatctttcttctgacaatggagattgagaatatcgtggccaacaccgtgctgctgaaggcgagggaaggaggcggagggaagcgtaatggacggagcaagaaatggaaggagatgctgaaattgcctcatatcagtcagtgtgaggaactgagacgcacaattgagagggattatacaagcctttgtgaaaagcagccaattgggcgtcagttgtttcgtcaattctgtgacactcagcccaaattacggcgctgtatagagttcctggattctgtggcaatgtatcaggtggctccggatgagaagaggagagacgccggtttaaatattttagacacatattttaataacgggtcagccgctcatctaccagagatggctcaggatgtggtcggggaatgcaggcagaaactggaacagagtccatgtaaagagctgttccaggagtgtaccaggatagttcgtgaatatttaagtggagcgccattctcggcttatcaggagactatgtacttttcccgttttcttcagtggaagtggttggagaggcaaccagtaaccaaaaacacattcagacattacagggtattgggcaagggcggatttggtgaggtttgtgcctgccaggttcgtgccactggtaagatgtacgcctgtaaaaagctggagaagaaacgtgtgaagaaaagaaaaggtgaagcaatggcattaaatgaaaaacgcattttagaaaaagttaacagtagttttgtagttagtttggcatatgcgtatgagactaaggacgctctatgtttggtgttgaccataatgaacggtggtgatctgaagtttcatatctataacatgggaaactcaggtttcgatgagcagcgggccatcttttacgcggcagagatctgttgcggattagaggatctgcatcgagagcggatcgtctacagagacttgaaaccagaaaatattctgctagatgatcgtgggcacatccgtatttctgatctgggactggccgtgcaaattccagagggcgagacgatacgagggcgtgttggcacagtggggtacatggcaccagaggtcatccagaacgagagctacaccttcagtcctgactggtggggtttgggctgtctaatttacgaaatgattcagggtcagtctcctttccgtcgccgtaaagagcgagtgaagagagaggaagtggaccggcgagtttgtgaggaccaggaggaatatactgagaagttctcagaagatgctaaagacatctgccgtcagttgttatgtaaagatccaaaggagcgcttgggctgtcggggcagtggagcggccgaagtcaaactgcatcccattttccgcaacatcaacttcaagcggctggaggccaacatgctggacccgccgttctgtccagacccgcgagcagtttactgtaaggacgtcctggacatagagcagttttcgacagtcaaaggtgtaaacctggacccaacagatgacgatttctaccacaagtttgtaacagggagtgtctccatcccatggcaaaatgagatgattgagatggagtgttttaaggagatcaacgtgttcgagatggacggagcgctgtgtgctgatctggatgtgaacagacccaatccagcaccgaagcgaggattcttctaccgtttatttcaccgagaggcaagtggtagtgcatcgcccgccgtgctcagagggggaggggcttgaggtttgttttgggagtgccaacagtgaggaggaggagaaagaggagaaggaggaggagccatcttgacttaaaaccactcccacttcccatcagctgccaatcagaatgctaaacgtatgaatgttcaactgtatttatgaactgtatcaaactgtatcataattaaagaaaagggtatgagtttaaagaattttgtacatttgtacttgaattatgtgtagatgtatattttcactaaaggttgtattgtacaaaaagccatagaaataccacttgaatgcatatataatgtttttattgttattatttttcatctgtactgaatatgaaatgtgtatttctttttaagcacaatataaacactcaccttttgtttccaatgctaacgctctgttagcactgcctttgtttgtatgttaacttagatcgtttggctggacagtaaaccgtgcttcttttgtacagcagggtaagatcaatcacacataaagagggagcagtacaaatgatctattcatatcatatcatttacagtggtcaaccagaatgctaatatcaaaagcaggggtggctgtggtcaaataaaaacaaaaaaacaaactgtatatagcctagatatataaaaacgaggttcaaaagtctgagaataccaagtaaaatgcttcaaaatatatattcagaaaaatctggactgaaaatgaattgcgtgcaataataacacctttgttaagcaatatgtggctaattttgagattcaaactgttaggaaatatactcataatttcaaaattagcctaatgttgatgtattaaaacatattaatgctttattgccaccttcactcattcccccccaatttttgtcaaatttgacatgcctttttcttcaacccaggctcattctgaaaatgtacccccgtatacatttctggagagcactaattacatccctggagctacgtttttgcttttttttttcgaattccccagaggccgctgtgtatgctttttaaaatctaaattttttctagcaagtaccatttaaacctgctgttcttgcgtaaatccaccagaggctgctgtcaactgactgactgaccgactgacccaccctcctccttccctaaacccaaccaatagtgtttccaatagcaccgattgacccgccttcgctaaccacaaccgacagtttaaaaagcaatccaaaaaaagaaaagcccttgcctgatttttaccatgttttcagatttttccacattctcaccgttatttacttcattttattttttggcttctgtttttgtcttacccgctttctggaaccattcgaaccctgtcgttggggtcaactcctctctgcgtctcaagtcatacatgactcgacatacatggcgagctgctggacaaactgataacagtagaaacgctgtttacatggaagtcagcggtcagctggaaagcgagaaaagaccaccctgtagccttcattttaaagacgaaatgctgccatatgtacctctggctacataattcatgatcttcttaaatgtatatagggctatgtttttagagtgagcctatgttgttgttgttgttttgtaaatgtttatttttagggctaaattgaatatataactccagattttcagtgtgtcctcagacttctgaaccccacctgtgtgttgacctcattgacaagctgtctggattttgtacaaaaggaaagtgttcatacatatgttaatgataatgggtgtgccaatagctagtgccaatagcctcaaaacaaccagatgattaataggtccaatccaatgactaataacaatgtgtaacttgcgggttgtggaagtgtaacgtcattcattttctccatagggaatttttttttttaaactgataactgcagtcttgttgtgagctctgaggttcttgattgtatatgctgttgctgaacttgtctattcacattaaatcactttattatttcaacagtcatttttgattgtagaatttggccataacaacgacattacccatgatgctgtgcagaaaaccaaccaatcagacagttgcagcgtttctgattgtagaatctggcaaaagcaaccaatttacccatgatactgtgcagaaaactcaccaatcagactgttgcagtgtttttgatcatagaattgggccataacaaccacattaaacattatgctgtgcagaaaacccaccaatcagagagttgtagcattttacattttagaatttggccataacaacaacattacccatgatgctatgcagaaaacccaccaatcagacagttgcagctgacaaagcaactttacattatatcactttattatagtcaattttgatcgtagaattgggccataacaacaacattacccgtgatgctgtgcagaaaagccaccaatcagacagttgcagcttttttgattgtagaattggtccataacaactacattacccatgatgctgtgcattaaagccaccaatcagacagttgcagcgattttaattgtagaatttcgccataactacaatacccatgatgctgtgcagaaaacctatcaatcagaccgttgcagcgtttttgattgtagaatttggccaaaacaactacattacccatgatgctgtgcagaaaagttaccaatcggacagttgctgctaacaaagcaacttcagattaaatcaccttttttattttaacgatcatttttgattgtagaatctggccataacaactacattacccaacatgctgtgtgcataaaacccaccaatcagacagttgcagcattttttattatagaatttggctataacaactacattacccatgatgctgtgcacaaaacccaccaatcaggcacttacagcatttttgatagtagaatttggccataacaactacattacccatgatgctgtgcagaaaatccatcaatcagacagttgctgcatttttgattgtagaatttggccataacagccacattacccatgatgctttgcagaaaacccaccaatcagacagttgtagcgttttaatggtagaattgggacacaacaaccacattacccatgatgctgtgcagaaaacccaccaattagacagttgcagctaacatagcacgtcaagagatctttaactccaaaaacttccatgaagcaccccaaaatattttccaaaaaagcttaaaatatggataaaacaaacacctttaattgaacaattttttatttcttaatcgttttaatctgattatgttttgtgcaatgctttatgggattgtagttctttttctcatttaacctgttcagtatgtcttgtacttcttctcagatttgtaaggttgtgattcacctcagatgattagacagttttattactcaaaaaaataaatattttttaatccctatggtaaaatgaacaggcaaatactttcagaaaaagtgaacgtcagcactagtcgtaaaattgttctttcattaaattcatgcagctttaacgaaatgcatgaaaccaataatctctgtgcatcgggtgactaatcccagttttggttacgcattaacaagacacatatgtatgttaattaacgtagtcaccgtagctttcagatgcatcaacgacagggaaccaggcatgttgttcaacgatagactaatatgagttttttttttcagtggcagataatcgatatctagtacctagtaatatggacatgtacgtaaataagtgactgaatgatggcatattacactatatttactcaaaacccacatacgattgaacatataaaatgtttactatccattaacatggctgttgttcggtttttggagttgatacaaggaaatgttaattactctagctatgtgtcagtagttttcaggccgctaatctgaaatgatttattttttccgttttgttgactgtttgctaatatccactctcatggttacatttagtgccatcaggaaactttaaaccaacgaaataagcttcatctttgaatttgctcttgaacgagctgtcgattttgccttaggtgtcatgtgttcggtcgacattccaaacggagtatcgatcgatcccttaacgataatcatatgatgtaattaatgactgtgcagtttgttcgttaaacacttgcacattttacattttgctttgtatgtatttgtgtggcatattttaatcgcaatgaaggcttccatttagtgatgggaattagcatctcgatcttacgagaaactattttacatgtcatcagaaaagtggctaacttgtacaaattcgtacggtttcatttgcatgaaaacataagatttttttaaaagtaggagtggccccaaaccccacccaaaacttattcctcattgggtggaattagtgaatcgtattaaaatgtacatatgagatcgtacaaattaatatgaattagccacttcatcaaaaagttacgaattgagaattgtgttggaattagtctcaaatgtattgttcataaattgggtaatattacatttgatttaaatggaggaagtgaaaattagttttgcttccattcagtggtgggaattagcagctcgatctcacgaggaactattttacatgtcagaaaagaggctaattcgtataaatgcatacaatttaatttgtatgaaaacgtacaatttttaaaaagtgggtgtggccccaaaccccacccctaaccctacccgtcattgggtggaattatcgaatcttattaaaatttacgaattagatcgtacaattaatacaaattagccactacatcaaaaagttacgaatttagaattgcgttggtattcaaatgtcaaatgtatcattcggaaattgcataatattacatttgaattaaatgaaggaagtggaaattagttttgatctaaaaaatgcttttgtctaacgcaccttcgaaatcagagtcccaaccaatggcgtgagtcgggggcggggctataacatgcttcagcactgacagtaaagctcaaataggaaccattattttacctccatcacccatttttgtctttatgaggatctttatggtcccgtttgcaaagctgtgaactgaaaacaactcctcttctgttattcccatatgtgtgcatgttctactgtgtccatggttttacaagaggatgagcactcactttagttgacaataaagttatgagttgaca
//
by
@meso_cacase at
DBCLS
This page is licensed under a
Creative Commons Attribution 4.0 International License (CC BY 4.0).
If you use GGRNA in your work, please cite:
Naito Y, Bono H. (2012)
GGRNA: an ultrafast, transcript-oriented search engine for genes and transcripts.
Nucleic Acids Res., 40, W592-W596.
[Full Text]