LOCUS       NM_001002159            1944 bp    mRNA    linear   VRT 14-FEB-2025
DEFINITION  Danio rerio tRNA nucleotidyl transferase, CCA-adding, 1 (trnt1),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001002159
VERSION     NM_001002159.2
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Giannelou,A., Wang,H., Zhou,Q., Park,Y.H., Abu-Asab,M.S., Ylaya,K.,
            Stone,D.L., Sediva,A., Sleiman,R., Sramkova,L., Bhatla,D.,
            Serti,E., Tsai,W.L., Yang,D., Bishop,K., Carrington,B., Pei,W.,
            Deuitch,N., Brooks,S., Edwan,J.H., Joshi,S., Prader,S., Kaiser,D.,
            Owen,W.C., Sonbul,A.A., Zhang,Y., Niemela,J.E., Burgess,S.M.,
            Boehm,M., Rehermann,B., Chae,J., Quezado,M.M., Ombrello,A.K.,
            Buckley,R.H., Grom,A.A., Remmers,E.F., Pachlopnik,J.M., Su,H.C.,
            Gutierrez-Cruz,G., Hewitt,S.M., Sood,R., Risma,K., Calvo,K.R.,
            Rosenzweig,S.D., Gadina,M., Hafner,M., Sun,H.W., Kastner,D.L. and
            Aksentijevich,I.
  TITLE     Aberrant tRNA processing causes an autoinflammatory syndrome
            responsive to TNF inhibitors
  JOURNAL   Ann Rheum Dis 77 (4), 612-619 (2018)
   PUBMED   29358286
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   DeLuca,A.P., Whitmore,S.S., Barnes,J., Sharma,T.P., Westfall,T.A.,
            Scott,C.A., Weed,M.C., Wiley,J.S., Wiley,L.A., Johnston,R.M.,
            Schnieders,M.J., Lentz,S.R., Tucker,B.A., Mullins,R.F.,
            Scheetz,T.E., Stone,E.M. and Slusarski,D.C.
  TITLE     Hypomorphic mutations in TRNT1 cause retinitis pigmentosa with
            erythrocytic microcytosis
  JOURNAL   Hum Mol Genet 25 (1), 44-56 (2016)
   PUBMED   26494905
REFERENCE   3  (bases 1 to 1944)
  AUTHORS   Amsterdam,A., Nissen,R.M., Sun,Z., Swindell,E.C., Farrington,S. and
            Hopkins,N.
  TITLE     Identification of 315 genes essential for early zebrafish
            development
  JOURNAL   Proc Natl Acad Sci U S A 101 (35), 12792-12797 (2004)
   PUBMED   15256591
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CU468905.10.
            
            On Jun 24, 2024 this sequence version replaced NM_001002159.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC152183.1, AY648718.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA3505370,
                                           SAMEA3505373 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-121               CU468905.10        181756-181876       c
            122-268             CU468905.10        181380-181526       c
            269-462             CU468905.10        180587-180780       c
            463-601             CU468905.10        180354-180492       c
            602-728             CU468905.10        180120-180246       c
            729-922             CU468905.10        178675-178868       c
            923-1176            CU468905.10        177295-177548       c
            1177-1944           CU468905.10        173948-174715       c
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /strain="Tuebingen"
                     /db_xref="taxon:7955"
                     /chromosome="6"
                     /map="6"
     gene            1..1944
                     /gene="trnt1"
                     /gene_synonym="zgc:86645"
                     /note="tRNA nucleotidyl transferase, CCA-adding, 1"
                     /db_xref="GeneID:415249"
                     /db_xref="ZFIN:ZDB-GENE-040625-176"
     exon            1..121
                     /gene="trnt1"
                     /gene_synonym="zgc:86645"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    118..120
                     /gene="trnt1"
                     /gene_synonym="zgc:86645"
                     /note="upstream in-frame stop codon"
     exon            122..268
                     /gene="trnt1"
                     /gene_synonym="zgc:86645"
                     /inference="alignment:Splign:2.1.0"
     CDS             130..1425
                     /gene="trnt1"
                     /gene_synonym="zgc:86645"
                     /EC_number="2.7.7.72"
                     /codon_start=1
                     /product="CCA tRNA nucleotidyltransferase 1,
                     mitochondrial"
                     /protein_id="NP_001002159.2"
                     /db_xref="GeneID:415249"
                     /db_xref="ZFIN:ZDB-GENE-040625-176"
                     /translation="

MWAKLFLRPSFVNRVHLTWSCRALLTMQLKTKEFESLFTDGLVGLAEIFQKNQFELRIAGGAVRDLLSGKRPEDVDFATTATPEEMKSMFQTAGVRMINNKGEKHGTITARLHEENFEVTTLRVDVQTDGRHAEVEFTTDWQKDAERRDLTINSMFLGLDGTLYDYFQGYEDLKNRKVRFVGSASLRIQEDYLRILRYFRFYGRVAAEPGQHEPETLEAIRENARGLAGISGERIWVELKKMLVGNHAGHLLELVYELGLAQYTGLPADGDVEEMKQVWQRAHVSSPKPMTVLAALFRKQADVENLDQRLKVSREEKNLGLFLVKYRRDLVKGHDEHDTMKPYTDFITDSREPDTQSKVLELLKYQGENKLLDELRRWSIPRFPVSGHDLRKLGYTSGKEIGTILQELRDMWKKSRYQMSKDELLSTLSQS"

     transit_peptide 130..222
                     /gene="trnt1"
                     /gene_synonym="zgc:86645"
                     /note="Mitochondrion. /evidence=ECO:0000255; propagated
                     from UniProtKB/Swiss-Prot (F6NXI9.1)"
     mat_peptide     223..1422
                     /gene="trnt1"
                     /gene_synonym="zgc:86645"
                     /product="CCA tRNA nucleotidyltransferase 1,
                     mitochondrial. /evidence=ECO:0000255. /id=PRO_0000458163"
                     /note="propagated from UniProtKB/Swiss-Prot (F6NXI9.1)"
     misc_feature    262..1404
                     /gene="trnt1"
                     /gene_synonym="zgc:86645"
                     /note="tRNA nucleotidyltransferase/poly(A) polymerase
                     [Translation, ribosomal structure and biogenesis]; Region:
                     PcnB; COG0617"
                     /db_xref="CDD:440382"
     misc_feature    574..576
                     /gene="trnt1"
                     /gene_synonym="zgc:86645"
                     /note="May assist in discriminating ATP from CTP.
                     /evidence=ECO:0000250|UniProtKB:Q7SIB1; propagated from
                     UniProtKB/Swiss-Prot (F6NXI9.1); other site"
     misc_feature    697..699
                     /gene="trnt1"
                     /gene_synonym="zgc:86645"
                     /note="Involved in nucleotide selection.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (F6NXI9.1); other site"
     exon            269..462
                     /gene="trnt1"
                     /gene_synonym="zgc:86645"
                     /inference="alignment:Splign:2.1.0"
     exon            463..601
                     /gene="trnt1"
                     /gene_synonym="zgc:86645"
                     /inference="alignment:Splign:2.1.0"
     exon            602..728
                     /gene="trnt1"
                     /gene_synonym="zgc:86645"
                     /inference="alignment:Splign:2.1.0"
     exon            729..922
                     /gene="trnt1"
                     /gene_synonym="zgc:86645"
                     /inference="alignment:Splign:2.1.0"
     exon            923..1176
                     /gene="trnt1"
                     /gene_synonym="zgc:86645"
                     /inference="alignment:Splign:2.1.0"
     exon            1177..1944
                     /gene="trnt1"
                     /gene_synonym="zgc:86645"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

tgttattacgcaagtgtcccggaagcagttggccacgcccttacgtgaatgtctgtcatggctgctcgcagcgtgagtgaaactaccgatgtgctaaaatagcatctggtgttttcctaaggtccaccgatgtgggctaaattatttctacgcccttcgtttgtcaaccgggttcatctcacgtggagttgcagggctttattgacgatgcagctgaaaactaaagagtttgagtctttatttaccgacggactcgttggtcttgctgaaatatttcaaaagaaccagtttgagctgaggattgccgggggtgcggtgcgggaccttctgtctggaaaacggcctgaagatgtggattttgccacaacagccactccagaggagatgaagagcatgttccagacagcaggagtccgcatgatcaacaataaaggagagaaacatggcaccattactgccaggcttcatgaggagaactttgaagtgaccacattgcgagtggatgtccagactgatggacggcatgcagaagtggagtttaccactgattggcagaaagatgccgagcgcagagacctcaccatcaactccatgtttctcggtctggatgggactctgtatgattattttcaaggatatgaggaccttaaaaacagaaaagtgaggtttgttggaagtgcttcactacggatccaagaggactacctgcgaattctgagatatttcagattctatggacgagtggcagctgaacctggccaacatgaaccagagactttggaggcaataagagaaaacgctagaggattggctggaatctcaggcgagaggatctgggtggagttgaagaagatgctggttggaaatcatgctggtcacctgctggaactcgtctatgagttgggtttagcccagtacacaggtcttccagcagatggcgatgtggaggaaatgaagcaggtctggcagagggcccatgtcagctctcctaaacccatgaccgtcctggcagctctcttcagaaagcaggccgatgtggagaacctggaccaaagactgaaggtgtccagagaagagaaaaacctgggcctcttcctggtcaaatacagacgagatctcgtcaagggacacgacgaacatgacacaatgaaaccatacactgatttcatcactgattctcgagagccggatacccagagtaaagtactagagcttttgaagtatcaaggagaaaataaactcttagatgagctgcgcaggtggtcaatcccacgattcccagtgagcggacatgacctacgcaagctgggttacacctcaggtaaagagatcggcaccatcctgcaggagctcagagacatgtggaagaagagccgctatcagatgagcaaagacgaacttctgtccacactcagtcaatcctgatgacataaacactcacacacacacactcacacacactagagtcaaaagttgtgcatgcatgagctataatgcattacgagtctaaagatgaacctctgtagtgtcttatatcgaacaatcaggggagtttaatgcttattattcacaaaatttcctttcattttcctcttataattggcagtttggtgcgacaagattacgttcattcattaatataattgctttgaagaaaggatgtgtctaaactgaatgcctccgtaatagaaagttcattttgtgctttttattatttgatgcaggattttgccctttcatgagagtgaagtggtgtaattattacagtaatgggagcaatttatctgtcctgcaggatgacattttctcatatgaagctgtacccatggtttcattatgaggaaaaattgtccccttgtcagtggttcatatagttgttattagaaggttatgtaaccaactctcaaggtgtttactttacaataaagtagcaaacatcacgtc

//