LOCUS       NM_118426               6907 bp    mRNA    linear   PLN 20-OCT-2022
DEFINITION  Arabidopsis thaliana separase (ESP), mRNA.
ACCESSION   NM_118426
VERSION     NM_118426.4
DBLINK      BioProject: PRJNA116
            BioSample: SAMN03081427
KEYWORDS    RefSeq.
SOURCE      Arabidopsis thaliana (thale cress)
  ORGANISM  Arabidopsis thaliana
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; malvids; Brassicales; Brassicaceae;
            Camelineae; Arabidopsis.
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Mayer,K., Schuller,C., Wambutt,R., Murphy,G., Volckaert,G.,
            Pohl,T., Dusterhoft,A., Stiekema,W., Entian,K.D., Terryn,N.,
            Harris,B., Ansorge,W., Brandt,P., Grivell,L., Rieger,M.,
            Weichselgartner,M., de Simone,V., Obermaier,B., Mache,R.,
            Muller,M., Kreis,M., Delseny,M., Puigdomenech,P., Watson,M.,
            Schmidtheini,T., Reichert,B., Portatelle,D., Perez-Alonso,M.,
            Boutry,M., Bancroft,I., Vos,P., Hoheisel,J., Zimmermann,W.,
            Wedler,H., Ridley,P., Langham,S.A., McCullagh,B., Bilham,L.,
            Robben,J., Van der Schueren,J., Grymonprez,B., Chuang,Y.J.,
            Vandenbussche,F., Braeken,M., Weltjens,I., Voet,M., Bastiaens,I.,
            Aert,R., Defoor,E., Weitzenegger,T., Bothe,G., Ramsperger,U.,
            Hilbert,H., Braun,M., Holzer,E., Brandt,A., Peters,S., van
            Staveren,M., Dirske,W., Mooijman,P., Klein Lankhorst,R., Rose,M.,
            Hauf,J., Kotter,P., Berneiser,S., Hempel,S., Feldpausch,M.,
            Lamberth,S., Van den Daele,H., De Keyser,A., Buysshaert,C.,
            Gielen,J., Villarroel,R., De Clercq,R., Van Montagu,M., Rogers,J.,
            Cronin,A., Quail,M., Bray-Allen,S., Clark,L., Doggett,J., Hall,S.,
            Kay,M., Lennard,N., McLay,K., Mayes,R., Pettett,A.,
            Rajandream,M.A., Lyne,M., Benes,V., Rechmann,S., Borkova,D.,
            Blocker,H., Scharfe,M., Grimm,M., Lohnert,T.H., Dose,S., de
            Haan,M., Maarse,A., Schafer,M., Muller-Auer,S., Gabel,C., Fuchs,M.,
            Fartmann,B., Granderath,K., Dauner,D., Herzl,A., Neumann,S.,
            Argiriou,A., Vitale,D., Liguori,R., Piravandi,E., Massenet,O.,
            Quigley,F., Clabauld,G., Mundlein,A., Felber,R., Schnabl,S.,
            Hiller,R., Schmidt,W., Lecharny,A., Aubourg,S., Chefdor,F.,
            Cooke,R., Berger,C., Montfort,A., Casacuberta,E., Gibbons,T.,
            Weber,N., Vandenbol,M., Bargues,M., Terol,J., Torres,A.,
            Perez-Perez,A., Purnelle,B., Bent,E., Johnson,S., Tacon,D.,
            Jesse,T., Heijnen,L., Schwarz,S., Scholler,P., Heber,S., Francs,P.,
            Bielke,C., Frishman,D., Haase,D., Lemcke,K., Mewes,H.W.,
            Stocker,S., Zaccaria,P., Bevan,M., Wilson,R.K., de la Bastide,M.,
            Habermann,K., Parnell,L., Dedhia,N., Gnoj,L., Schutz,K., Huang,E.,
            Spiegel,L., Sehkon,M., Murray,J., Sheet,P., Cordes,M.,
            Abu-Threideh,J., Stoneking,T., Kalicki,J., Graves,T., Harmon,G.,
            Edwards,J., Latreille,P., Courtney,L., Cloud,J., Abbott,A.,
            Scott,K., Johnson,D., Minx,P., Bentley,D., Fulton,B., Miller,N.,
            Greco,T., Kemp,K., Kramer,J., Fulton,L., Mardis,E., Dante,M.,
            Pepin,K., Hillier,L., Nelson,J., Spieth,J., Ryan,E., Andrews,S.,
            Geisel,C., Layman,D., Du,H., Ali,J., Berghoff,A., Jones,K.,
            Drone,K., Cotton,M., Joshu,C., Antonoiu,B., Zidanic,M., Strong,C.,
            Sun,H., Lamar,B., Yordan,C., Ma,P., Zhong,J., Preston,R., Vil,D.,
            Shekher,M., Matero,A., Shah,R., Swaby,I.K., O'Shaughnessy,A.,
            Rodriguez,M., Hoffmann,J., Till,S., Granat,S., Shohdy,N.,
            Hasegawa,A., Hameed,A., Lodhi,M., Johnson,A., Chen,E., Marra,M.,
            Martienssen,R. and McCombie,W.R.
  TITLE     Sequence and analysis of chromosome 4 of the plant Arabidopsis
            thaliana
  JOURNAL   Nature 402 (6763), 769-777 (1999)
   PUBMED   10617198
REFERENCE   2  (bases 1 to 6907)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2022) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 6907)
  AUTHORS   Krishnakumar,V., Cheng,C.-Y., Chan,A.P., Schobel,S., Kim,M.,
            Ferlanti,E.S., Belyaeva,I., Rosen,B.D., Micklem,G., Miller,J.R.,
            Vaughn,M. and Town,C.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Plant Genomics, J. Craig Venter Institute,
            9704 Medical Center Dr, Rockville, MD 20850, USA
  REMARK    Protein update by submitter
REFERENCE   4  (bases 1 to 6907)
  AUTHORS   Swarbreck,D., Lamesch,P., Wilks,C. and Huala,E.
  CONSRTM   TAIR
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2011) Department of Plant Biology, Carnegie
            Institution, 260 Panama Street, Stanford, CA, USA
COMMENT     REVIEWED REFSEQ: This record has been curated by TAIR and Araport.
            This record is derived from an annotated genomic sequence
            (NC_003075).
            
            On Sep 12, 2016 this sequence version replaced NM_118426.3.
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="Arabidopsis thaliana"
                     /mol_type="mRNA"
                     /db_xref="taxon:3702"
                     /chromosome="4"
                     /ecotype="Columbia"
     gene            1..6907
                     /gene="ESP"
                     /locus_tag="AT4G22970"
                     /gene_synonym="AESP; EXTRA SPINDLE POLES; F7H19.150;
                     F7H19_150; homolog of separase; RADIALLY SWOLLEN 4; RSW4;
                     SEPARASE"
                     /note="Encodes a separase (ESP), homologous to human and
                     mouse separase protein. Separase is a capase family
                     protease required for the release of sister chromatid
                     cohesion during meiosis and mitosis. Arabidopsis separase
                     contains a predicted 2Fe2S-ferredoxin domain that is not
                     present in the proteins of other organisms. Also contains
                     a putative EF-hand calcium binding domain. Mutant seeds
                     exhibited embryo arrest at the globular stage. The
                     endosperm also exhibited a weak titan-like phenotype.
                     Transgenic plants expressing AESP RNA interference (RNAi)
                     from the meiosis-specific DMC1 promoter exhibited
                     alterations in chromosome segregation during meiosis I and
                     II that resulted in polyads containing from one to eight
                     microspores. Plays an essential role in embryo
                     development. Required for the removal of cohesin from
                     meiotic chromosomes and establishment of meiotic nuclear
                     domains. This gene was also identified through the rsw4
                     mutant. Lines carrying recessive, temperature-sensitive
                     mutations exhibit reduced anisotropic growth at 30 degrees
                     Celsius. Microtubules and cellulose microfibrils are not
                     depleted or disoriented in the mutants at the restrictive
                     temperature."
                     /db_xref="Araport:AT4G22970"
                     /db_xref="GeneID:828396"
                     /db_xref="TAIR:AT4G22970"
     CDS             99..6641
                     /gene="ESP"
                     /locus_tag="AT4G22970"
                     /gene_synonym="AESP; EXTRA SPINDLE POLES; F7H19.150;
                     F7H19_150; homolog of separase; RADIALLY SWOLLEN 4; RSW4;
                     SEPARASE"
                     /inference="Similar to RNA sequence,
                     EST:INSD:ES029721.1,INSD:ES078486.1,INSD:AV555708.1,
                     INSD:ES086031.1"
                     /inference="Similar to RNA sequence, mRNA:INSD:AY823256.1"
                     /note="homolog of separase (ESP); FUNCTIONS IN: peptidase
                     activity; INVOLVED IN: in 6 processes; LOCATED IN:
                     nucleus; EXPRESSED IN: 16 plant structures; EXPRESSED
                     DURING: 7 growth stages; CONTAINS InterPro DOMAIN/s:
                     Peptidase C50, separase (InterPro:IPR005314); BEST
                     Arabidopsis thaliana protein match is: unknown protein
                     (TAIR:AT5G28550.1); Has 401 Blast hits to 372 proteins in
                     177 species: Archae - 0; Bacteria - 2; Metazoa - 98; Fungi
                     - 137; Plants - 87; Viruses - 0; Other Eukaryotes - 77
                     (source: NCBI BLink)."
                     /codon_start=1
                     /product="separase"
                     /protein_id="NP_194028.2"
                     /db_xref="GeneID:828396"
                     /db_xref="TAIR:AT4G22970"
                     /db_xref="Araport:AT4G22970"
                     /translation="

MASSGDDLRLLSLIDVGDNVFSSFSDYLKPFSTLSTSRKKQDRATTIRALAKQFLPFLNKSISLLPKRLSVANSDKEARESALDLFRAYELCLDCLELVSAQLACKPHTVQSQRLRMIHCLDVWGLYENVYTEAFKVLEKLRGSDSKSRKSRLLPEVQDGDAEMALVVVDAVAAIFRAVAMSQQLDDKRYRKVLLLLEEVGGWLRVLDAKVYEKLHRAMVTSMGKCAVSLVREAERFNGDLVISFCDLTVKEHYKSALSKDRVYKFAREVLSVLFGFKDRKMSVTIDISMSVLRSLSCQFEDESNENLMEFFDLVDYCAHKFRAAGDMYCAKVSKKLNEMAAIFVEAIPQLNLVLRLYSTGLSITVCNSKLGEIKLEDSTDDWKIQAMFDDDARWQSLVSLLGMVDSYSGDEGNQTGSSSIGGHRNYNNKTHDSCKDRNKITCWPQYVDALKFLCQPLADFIYSVKRKIVLETEMSCASAHLITIHDAFLQFCDGCLFLQRCTSDKGDREIANNKAFLNAAMGAFIVSLRTQLKLEISAHLVEDVIGSPWIQSQELKYLIATLYNIGIVLYRNKELNKACEALKLCSKVSWRCVELHCHMFVNQSSSSDNDLSEDAIMDFVGEACNRCAFYLDILQKCSRRKIRQNIVHILENWLSAEHLIRRLPGPEAIVKQWVKIERECHTDLDAAGSCTTLYSLLSSSQKKSKRGIGKILEQELLAYDRVLPLRSNLGQQTRIKIADILLKDVYVTEDMHIERARILIWKARMTRTSGTEHITECICFLSEAISILGELHHGPNEEGSPSSHMLPIAYCLRAFCTQEADPNSKKVFQDISTSLNLWLRILSLDDSGDSLPTENIIPLLYNMIDLMSVKGCTELHHHIYQLIFRLFKWKNVKLEVCLAMLWECRRLSHALCPSPISDAFIQTLSENCADKSTCIDFWMDCLKDSKAKLIGFQQNFHDLHNKDEGPFQSDITIDDIKDAASELISSASLSGNSSFAAAYLYYDLCERLISFGKLSEALSYAKEAYRIRTLIFQDKFKYTAEKHIEKHNEDGKISEIRTFSIKNFQVYRLLATDFWPCGNFLWDINRCYLSPWSVLQCYLESTLQVGILNELIGNGLEAETILSWGKAFSCSQSLFPFVVAFSSALGNLYHKKQCLDLAEKELQNAKEILIANQRDFSCVKCKLKLEVTLDKQLGDISRKQIDRVSQTDGFLHAESLFSAALGKFCCSAWKSCIRSHGEEIAEEIVIDRNGGEGLGHNSSKTKLSIKEPPGNRGSRRGGRANKTCLSKDQDLISEPTSRLTRSMRHSLREQCQNRSNVPEVVSKKPNLCDRSVGSRGERVLLDTSNALPGFCICYKEKRQQCLSEEVTESGSLNNLVSLKWELCHRKLASSILVSLGKCLGDSGRIHLAHEALLHSISVLFKSTWSSHNQPSVSQLLEFIGKEVTRDVFAVDRAIILYNLCWLNLRNYHCRKSRSICCDLFHIPFTKLVSWLMLAFVLSGEVPILFQKVSRLLASLYLLSSSNSEFTFESDGNELSASHWVSFFHQASLGTHLSYHFISNLSQKHKSQCLSDKECTEATCSSCMVPEDLDLPRLAPDRTQDLVQFAKEFFINLPSSTIICISLLGGALNQLLQELMHIRSPVCAWVLISRLNPESQPVATLLPVDSIVEDMSDDSANLSSTEATQVKSLKGPWLCPWGTTVVDEVAPAFKSILEESHSSSSTTEEDTIESRGLWWKKRKKLNHRLGIFLRNLEASWLGPWRCLLLGEWSNYKLPDSAQKKLVNDLKSKCKMEVNEMLLKVILGGGTDNFKGEACVAQLSLRNGCYVGRGGYLYEEDSCKTPTAASNISESRHELALKLIHDAASKLGQQDGHENREPIILVLDPEVQMLPWENIPILRKQEVYRMPSVGCISAVLKKRSLQGEPAKSHVASFPLIDPLDSFYLLNPGGDLTDTQVTFESWFRDQNFEGKAGSEPSAIELTEALETHDLFLYFGHGSGAQYIPRREIEKLDNCSATFLMGCSSGSLWLKGCYIPQGVPLSYLLGGSPAIVATLWDVTDRDIDRFGKALLEAWLQERSDSSSEGGCSQCESLANDLAAMTLKGTKRSRKPSSRNKPAQSDVDGSGKIECNHKHRRKIGSFIAAARDACNLQYLIGAAPVCYGVPTGITRKKGIDALLPSSSR"

     misc_feature    5019..6278
                     /gene="ESP"
                     /locus_tag="AT4G22970"
                     /gene_synonym="AESP; EXTRA SPINDLE POLES; F7H19.150;
                     F7H19_150; homolog of separase; RADIALLY SWOLLEN 4; RSW4;
                     SEPARASE"
                     /note="Peptidase family C50; Region: Peptidase_C50;
                     pfam03568"
                     /db_xref="CDD:427370"
ORIGIN      

caacgaatgagattttgaaattagaagtgactgaaactgtgtaagacctagaaaatgtgcatcttcaattagcgggaactctcaaaatttcgaaatcaatggcgtcctctggcgacgacctccgtcttctctctctcatcgacgtcggcgataatgtcttctcttccttctccgattaccttaaacctttctctactctatccacttcccggaaaaaacaagaccgagcaaccacgattcgagccctagctaagcaattccttcctttcctcaacaaatcgatctctctcctaccgaaacgtctctccgtcgcgaattctgataaggaagctcgtgaatcagcattagatctgttccgtgcttatgaactttgtttggactgtttagaattggtctctgctcaattagcttgtaagcctcacactgttcaatctcagaggctaaggatgattcattgtctcgatgtttggggtttgtatgagaatgtgtatactgaagcgtttaaggttctggagaagctaagaggctctgatagcaaatcccggaagtcccggttgttgccggaggttcaggatggagatgcggagatggctttagttgttgttgatgctgtggcggccatctttagggctgtggcaatgagtcagcagttagatgataagcggtatcggaaagttcttcttttgcttgaggaagtcggaggctggttaagggtcttagatgctaaggtgtatgaaaagttgcacagagcgatggtgacaagtatgggtaaatgtgctgtttctctcgttagagaagctgagcgtttcaatggggatttagtgatttctttctgcgacttaactgtgaaggaacattataaatctgcattatcaaaagatcgagtttataagtttgctcgcgaggtgctttctgttctgtttggatttaaggatagaaaaatgtcagtgaccattgacatttcaatgtctgtgttgcgcagcttatcttgccaatttgaggatgaaagtaatgagaatttaatggagttttttgacctagtcgattattgcgctcacaagtttcgagcagcaggagacatgtactgtgcaaaagtttcaaaaaaattgaatgagatggcagctattttcgtagaggcaataccccaacttaatttggttcttagactgtattcgaccgggctgtccatcacggtttgcaattccaagctcggagaaattaagctagaagattcaacagatgattggaagattcaagctatgtttgatgatgatgctagatggcaaagcttagtttctttactcggcatggttgatagttactcaggtgatgagggcaatcagactggttcatcatcgattggtgggcataggaactacaacaataaaacacatgatagctgtaaagacaggaacaagattacttgttggccacaatacgtagatgccttgaaattcttgtgccaaccgcttgcagactttatatattctgtgaaaagaaagattgtgctggagacagaaatgtcatgcgcctctgctcatctaattactattcacgatgcctttctccagttttgtgatggctgtctcttccttcagaggtgcacgtctgacaagggagatagagaaattgccaataacaaagctttcttaaacgcggctatgggtgctttcattgtctcattgagaacccaacttaaattggagataagtgcccatctagttgaggatgtaattggaagcccatggatccaatctcaagaactcaagtatctaattgcgacgctgtacaatattggtatcgttttgtacagaaataaggagctaaacaaggcttgtgaggcgcttaagctgtgctccaaggtatcatggagatgtgttgaactacattgtcatatgtttgtaaatcagtctagttcatctgataatgacttgtcagaagatgccattatggattttgtgggcgaagcatgtaataggtgtgcattctacctggatatactccagaaatgtagcagacgtaagattagacagaatattgttcatattctagaaaattggttgtcagctgaacatctgattagacggttaccaggccctgaggcaattgtaaagcaatgggttaagatagagcgggaatgtcacacggatctggatgcggcaggttcttgtacaactttgtactcattgttatcatcttctcaaaaaaagtcaaagcggggtatcggaaaaatccttgagcaggagcttcttgcctatgacagagtgttacctttaaggtcaaatctaggtcaacaaacgcgaatcaagatagcagatatcctcttgaaggatgtttatgtcactgaggatatgcatatagagagagcaagaattttgatatggaaagcacgaatgacaaggacatctggaactgaacatataaccgagtgcatttgttttctgtctgaagcaatctctatattgggtgagttacatcatgggccaaatgaagagggttctccatcttctcacatgttacctattgcgtattgcttgagagctttttgtacccaggaagctgacccaaactcgaagaaagtctttcaagatattagcacttcactaaatctctggctaaggattcttagcctagatgatagtggagacagtctaccaacagagaatataattccgttactgtataatatgattgatttgatgtcagtgaagggatgtacagagctccatcatcatatataccaactgattttcagattgtttaaatggaagaatgtcaaattggaggtctgccttgcaatgctgtgggaatgtagaaggcttagtcatgccttatgcccttcccccataagtgatgcctttatccagaccttatcagagaattgtgctgacaaatccacatgcattgatttctggatggattgcctgaaagattcaaaagcaaagttaattgggttccagcagaacttccatgatttacataataaggatgaaggtccctttcaatcagacatcacaattgacgatattaaagatgcagcatcagaactcatctccagtgcatcactctctggtaattcatcttttgcagctgcatatctctattatgatctctgtgaaaggctcatatcatttggaaaactttctgaggctctttcatatgcaaaagaagcctataggataaggactcttatatttcaagataaatttaagtatacagctgagaagcatatcgaaaaacacaatgaagatgggaaaatatcagaaatacggacttttagcataaaaaatttccaagtctacagattgttagcaactgatttttggccatgtggaaattttctctgggacatcaatcgctgctacctaagtccttggagtgtactccaatgttatttggaaagcacccttcaggttggaattctgaatgagctcatagggaatggactggaggcagaaaccattctatcatgggggaaagccttttcatgctcgcaaagtctattcccttttgtagttgcattttcttctgccttaggaaatctataccacaaaaagcagtgtctggatctggcagaaaaggaactccaaaatgcaaaagagatattaattgctaatcagcgagatttttcttgcgtaaagtgcaaactgaagttggaagttacattggataaacaacttggagacatatctcggaaacaaattgatagagtttcacagacagatggattcttgcatgctgaaagcttgtttagtgctgccctgggaaaattctgttgctcagcatggaaaagctgcattagatcacatggggaagaaattgctgaggagatagtgattgatagaaatggaggggaaggtttaggacataattcaagtaaaacaaagctcagtataaaggagccaccaggaaacagaggctccaggaggggtggaagagctaacaaaacttgtttgtcaaaggatcaggatttgatatctgagccaacatcaaggttgactcgatctatgcgtcactcacttagagagcaatgccaaaaccgttctaatgtgcctgaagttgtttcaaagaagcctaatttatgtgatagatctgttggttctaggggtgaaagggttttgttggacacgagtaatgcactccctggcttttgtatttgctacaaagaaaaacgtcagcagtgcctctcggaagaagtaacagaatctgggtctctcaacaatttagtaagtttgaaatgggaactctgccataggaagcttgcgtcctcaatacttgttagccttggaaaatgtttgggagactctggtagaattcatctagctcatgaggctttactgcatagtatctctgttttattcaaaagcacctggtccagccacaaccagccttctgtcagtcagttgctggaatttatcgggaaagaagttacaagagatgtatttgccgttgatcgtgcaataatattatacaacttgtgctggttgaatttgcggaattaccactgcaggaagagtaggtctatctgctgtgatctgtttcatatccctttcacaaaactagtgtcatggttaatgttagcatttgtactcagcggagaggttccaatattatttcaaaaggtttcaagattgcttgcctctttatatctgctttcttcctcaaattctgaattcacattcgaatctgatggaaatgagctgtcagcaagtcattgggtctcatttttccatcaagcctcgcttggtacccatctcagttaccatttcatttcaaatttgagccaaaaacacaaatcacagtgcctctcagataaagagtgcacagaggctacttgctcgagttgcatggttcctgaggatttggacttacccaggcttgctcctgaccgtactcaagatcttgtacaattcgctaaagaatttttcatcaatcttccaagctcaacaatcatctgcattagtttgcttggaggtgctttaaaccaattgttacaggagctaatgcatattcgttcacctgtttgtgcctgggtgctcatatcacgcctaaacccagaaagtcaaccggttgccacacttttacctgtagattcgattgtagaagacatgtcagatgacagtgcaaatcttagctctaccgaagcgactcaagtcaagagtttaaaggggccctggctttgtccatggggtaccaccgtggttgatgaggtagctccagcatttaaatcaatattggaggaaagccactcatcttcttctactactgaagaagacacaatagagagtaggggcctgtggtggaaaaagagaaagaaacttaatcatcgtctgggaatattcctaaggaatctagaagcttcctggctgggtccctggaggtgtctgcttctcggagaatggtcgaactacaaattgcctgactcagcgcagaaaaagctggtaaatgatctcaagtccaagtgcaaaatggaagtaaatgagatgcttttgaaggttattcttggaggtgggacggataactttaaaggagaagcatgcgttgctcagctttctttaagaaatggttgctatgtaggtagagggggatatctttatgaagaagatagctgtaaaactcctactgctgcgtctaatatatctgaaagtaggcatgaattggccttaaaactgatacatgacgcagcaagcaaactagggcaacaagatggacatgaaaatagagaacctatcattcttgtgttagatcccgaggttcagatgcttccttgggagaatattccaatactaaggaaacaggaggtgtaccgcatgccttccgtaggttgcatatctgccgtgctaaagaagcgcagtcttcaaggagagccagcaaaatctcatgttgcttccttccctcttattgatccgctagattcattttacttgttgaatcctggtggtgatctcaccgatacacaagtcacgtttgagagttggttcagagatcaaaactttgagggtaaagctgggtcagaaccaagtgctatagagttgacagaagcattagaaactcatgatctctttctatactttggtcatggaagcggagcacagtatatacctaggcgtgaaatagagaagctagataactgttctgcaactttcttaatgggatgcagtagtggttcactatggctaaaaggctgctacatcccacaaggtgtaccactttcttatctcctaggtggatctccagccattgtggcgactctatgggacgtaacagaccgagacattgatcggtttgggaaagcgttgttggaagcatggttgcaagagaggtcagattcttcttcagagggtggctgcagccaatgtgagtcattagctaatgaccttgcagcaatgactctaaaaggcaccaagaggtcaaggaaaccatcatcgcgtaacaagccagctcaatcagatgtggatgggtcagggaagattgagtgcaaccacaagcatagacgtaaaatcggttcattcatagccgcggctcgagatgcgtgcaacttgcaatacttgattggggctgcgcctgtctgttatggtgtgccaactggtatcactaggaaaaaaggaattgatgctcttcttccttcttcatctcgttagtaggttagagcatctcttaaaatcctcattgtttggtcgtataacaattttgagggagcaaataaatttaggagttaggacttcagatcagagattttatgacaacaacgcaagagaagagaaaaaaggagactctagtctagagatatatattcatccctgtttttttatataacaaaattgtaaaaaaaaatatcacatgatgtattgtcctcgaattctatgttataaaagtgttagtaatatatgatccatttttgtgtatt

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