LOCUS       XM_075827953            2680 bp    mRNA    linear   VRT 01-AUG-2025
DEFINITION  PREDICTED: Rhinoderma darwinii argonaute RISC catalytic component 2
            (AGO2), transcript variant X2, mRNA.
ACCESSION   XM_075827953
VERSION     XM_075827953.1
DBLINK      BioProject: PRJNA1298320
KEYWORDS    RefSeq.
SOURCE      Rhinoderma darwinii
  ORGANISM  Rhinoderma darwinii
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Amphibia; Batrachia; Anura; Neobatrachia; Hyloidea;
            Rhinodermatidae; Rhinoderma.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_134691) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_050947455.1-RS_2025_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.4
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/30/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Rhinoderma darwinii"
                     /mol_type="mRNA"
                     /isolate="aRhiDar2"
                     /db_xref="taxon:43563"
                     /chromosome="5"
                     /tissue_type="larvae"
                     /dev_stage="juvenile"
                     /geo_loc_name="Chile: Reserva Nacional Villarrica"
                     /lat_lon="39.4810 S 71.6749 W"
                     /collection_date="2020-12-06"
                     /collected_by="Claudio Azat"
     gene            1..2680
                     /gene="AGO2"
                     /note="argonaute RISC catalytic component 2; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 1 Protein, and 100% coverage of the annotated genomic
                     feature by RNAseq alignments, including 2 samples with
                     support for all annotated introns"
                     /db_xref="GeneID:142652302"
     CDS             64..2268
                     /gene="AGO2"
                     /codon_start=1
                     /product="protein argonaute-2 isoform X2"
                     /protein_id="XP_075684068.1"
                     /db_xref="GeneID:142652302"
                     /translation="

MACVSLQALHDALTGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCANPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFMCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPFVREFGIMVKDDMTDVTGRVLQPPSILYGGRVWEEPNAPLNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTELHLKTFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYTGLQLIVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA"

     misc_feature    <73..159
                     /gene="AGO2"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    187..339
                     /gene="AGO2"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    340..702
                     /gene="AGO2"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(496..498,541..543,583..585,595..597,649..651,
                     670..672,676..678)
                     /gene="AGO2"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    835..2139
                     /gene="AGO2"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1273..1275,1285..1287,1321..1332,1339..1341,
                     1363..1365,1372..1374,1384..1386,1396..1398)
                     /gene="AGO2"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1477..1479,1483..1485,1693..1695,2107..2109)
                     /gene="AGO2"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

gagttggaagttacactgccgggtgaaggaaaggatcgcatcttcaaagtagcgatcaaatggatggcctgtgtgagcttacaagccttacacgatgcgttgactggacgtcttcccagcgttccttttgagactatccaggcattagatgtagtgatgagacatttgccctctatgaggtacacgcctgtgggccgctcgttctttactgcatccgaaggatgtgcaaatccgcttggtggaggtagagaagtttggtttggcttccatcagtctgttagaccttccctatggaaaatgatgctcaacatagatgtatctgctacagcgttttataaagcacagccagtaatagagttcatgtgcgaagttctggactttaagagcatagaggaacaacagaaacctctgactgattcccaaagggttaagtttaccaaagaaataaaaggcttaaaagttgaaattacacactgtggacaaatgaaacgaaaatacagagtctgcaatgtgacgagacgaccagcaagtcatcaaacattccctctccagcaagagagcgggcagacggtggagtgcaccgtcgcacagtacttcaaggatagacacaaactagtattacgctaccctcatcttccatgtttacaggttgggcaggagcagaaacatacatacctccctcttgaggtatgcaacatagtggcgggacaaaggtgcattaaaaaactgaccgacaatcaaacctccactatgattcgagcaacagcccgatcagctccagatcgccaagaagagatcagtaaattgatgcgcagtgcaagcttcaatactgatccctttgtccgtgagtttgggattatggtgaaagacgacatgaccgatgtcactgggagagtccttcaacctccttcaattctttacggaggcagggtatgggaagagccaaatgctcccttgaataaggccattgctactcctgtgcaaggtgtatgggatatgaggaacaagcagtttcacaccggtattgaaataaaagtgtgggccattgcttgctttgctcctcagagacagtgtacagaacttcatctgaagactttcactgaacaattgaggaaaatctcccgggacgccggcatgcccattcaagggcagccatgtttctgcaaatacgctcaaggagcagatagcgtggagccgatgttcagacatctaaagaacacgtacactggtctacagctgattgttgtcattctccctgggaagacgcctgtctatgccgaggtgaagcgtgtgggggacactgtgttgggcatggcaacgcaatgtgtgcagatgaagaatgtgcagaggaccacgccacaaaccctgtctaacctctgcctgaagatcaacgtcaaattagggggagtcaataacatcctattaccccagggcagacccccagtgtttcagcagccagtcatattccttggagcagatgtcacccatcctcccgcaggggatgggaaaaagccatcaatcgcagcggttgtaggtagcatggatgctcatcccaaccgatactgtgccactgtaagagtgcagcagcaccgacaggagattatccaggacctggccgccatggtacgagagctgctcatccagttctacaagtctactcgcttcaagcccaccaggatcattttctatagggacggtgtttctgaggggcagttccagcaggtattgcatcatgagcttcttgccattagagaggcttgcattaaattggagaaggattatcagcctggaattactttcatcgttgtacagaaaaggcatcacacaagacttttctgtacagataggaatgaacgggttggaaaaagtggaaatattcctgctggtacgacagtggacacaaagatcacacacccatcagagtttgatttctatctgtgtagtcacgctggtattcagggaacaagccgaccctcgcactatcacgttctctgggacgataacagattctcctccgacgaacttcaaattctcacttaccagctgtgccacacttatgtccgctgcactcggtccgtgtctattcctgctcctgcatactacgcacacttagtggcttttagagccagatatcacctagtggataaagaacatgatagcgctgaaggcagccacacttctggtcagagtaatggcagagaccatcaagcacttgctaaggcagttcaagttcatcaggacacgctgcgtacaatgtactttgcttaactgctgctggaacccaacgatagaggcactacccgaaggagcattcacgccagaccagctgcacttacttgtaatgtagtctccgctacagtgacatccagcaaaagtgcaacctaaccatagccctgtcacactcataaggccctccgtatccaaccagccttccgtccagaccccgaatatattcttttatcctgaacttcatacctgcattgaatcatatgaggtttgtgaaatacatatataagcgtttacaaaaatttcccttttaaacaaaaaagttcaacttcactgttttctcagggctaacaaaaaaaacaaaacgctatgaaatacttttctctatggaaattctgtgatgaattatcagattaatgttatggcctaggaaaaaaaaaactagaggatgaaa

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