LOCUS       XM_075173163            3238 bp    mRNA    linear   VRT 11-JUL-2025
DEFINITION  PREDICTED: Calonectris borealis argonaute RISC component 4 (AGO4),
            transcript variant X3, mRNA.
ACCESSION   XM_075173163
VERSION     XM_075173163.1
DBLINK      BioProject: PRJNA1289127
KEYWORDS    RefSeq.
SOURCE      Calonectris borealis (Cory's shearwater)
  ORGANISM  Calonectris borealis
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
            Coelurosauria; Aves; Neognathae; Neoaves; Aequornithes;
            Procellariiformes; Procellariidae; Calonectris.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_134336) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_964195595.1-RS_2025_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.4
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/10/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Calonectris borealis"
                     /mol_type="mRNA"
                     /db_xref="taxon:1323832"
                     /chromosome="25"
     gene            1..3238
                     /gene="AGO4"
                     /note="argonaute RISC component 4; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 13
                     long SRA reads, 5 Proteins, and 99% coverage of the
                     annotated genomic feature by RNAseq alignments, including
                     19 samples with support for all annotated introns"
                     /db_xref="GeneID:142092757"
     CDS             96..2480
                     /gene="AGO4"
                     /codon_start=1
                     /product="protein argonaute-4 isoform X3"
                     /protein_id="XP_075029264.1"
                     /db_xref="GeneID:142092757"
                     /translation="

MVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDMEVTLPGEGKDQTFKVSIQWVSVVSLQLLLEALAGHLNEVPEDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKLTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQETSQELLYSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTQHTMYFA"

     misc_feature    105..359
                     /gene="AGO4"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    390..542
                     /gene="AGO4"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    543..905
                     /gene="AGO4"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(699..701,744..746,786..788,798..800,852..854,
                     873..875,879..881)
                     /gene="AGO4"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1044..2351
                     /gene="AGO4"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1455..1457,1467..1469,1503..1514,1521..1523,
                     1545..1547,1554..1556,1566..1568,1578..1580)
                     /gene="AGO4"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1659..1661,1665..1667,1905..1907,2319..2321)
                     /gene="AGO4"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

taatctggaggtggcttaaaactcctggtgttgacttgggtgtactctggtgcattgttgtttctcctaccttgctcatagaggtggtggatactatggtgagacacttcaagatgcagatatttggtgatcggcagcctggatacgatgggaaacggaacatgtatactgcacacccattacctattggccgggatagagtggatatggaggtgacacttccaggagaggggaaggaccagacatttaaagtgtccattcagtgggtgtcagtcgtcagccttcagttgctgctggaagctctggcagggcacttgaatgaagttcctgaagattctgtacaggcgcttgacgtaatcacacggcaccttccctccatgaggtacactcctgtgggtcgctcctttttctccccccctgaaggttattaccaccctctgggtggtggcagggaggtctggttcggtttccaccagtcggtcaggcctgccatgtggaacatgatgctcaacatcgatgtgtcagcaactgctttctatcgtgcgcagcctatcattgagttcatgtgcgaggtgttggacattcagaacatcaacgaacaaaccaagcctcttacggactcccagcgtgtcaaatttaccaaagaaatcagaggtctaaaagtggaggttacccactgtggccagatgaagagaaaataccgagtttgcaatgttactcggcgaccagccagtcatcagacgtttcctctgcagctggaaaatgggcaggctatggagtgtacagtagctcagtattttaagcagaagtacagtctacagctaaaatatcctcaccttccctgtctccaagtaggacaggaacagaaacacacatacttaccgcttgaggtgtgtaacatagtggcaggccagagatgtataaagaagctaacggacaatcagacgtcaactatgataaaagcaactgcaagatctgcaccggaccgacaggaagaaatcagcagactagtgaaaagtaacagtatggtcggtggacctgatccatatctgaaggagtttggtatcgttgtccataatgaaatgacggagttgacaggcagagttttgccagcaccaatgctgcaatatggaggcaggaacaagactgtggccacaccaaaccaaggcgtgtgggacatgagagggaaacagttctatgctggcattgagattaaagtttgggctgttgcctgttttgctcctcaaaaacaatgcagggaagacttactaaagagttttactgaccagctgcgcaagatctccaaagatgcagggatgccgatccaaggccagccctgtttctgcaaatacgcccagggtgcagacagcgtggagcccatgtttaaacacttaaaactgacttacgttggtctgcagctgatcgtggtgattctacccggaaagacacccgtgtacgctgaagtaaagcgggttggtgacactcttctaggaatggccactcagtgtgttcaggtaaagaatgtggtaaaaacctcaccccaaacgctgtccaacctgtgtctgaagataaatgcaaagcttggaggaatcaacaatgtgcttgtacctcatcaaaggccctcggtgttccagcagccagtgatctttttgggagcagatgtcactcaccctcctgctggggatggaaagaagccttccattgctgctgtggtaggcagcatggacggccatcccagccgttactgtgctacggtgcgcgtgcagacctcccgtcaggagacctcccaggagctgctgtacagtcaggaggtgatacaggacctgactaacatggtgcgagaactattgatccagttttacaaatccactcgtttcaagcccacaaggatcatttactacagagggggagtatcagaaggacagatgaagcaggtagcttggccagaactgatagcaatccggaaggcctgtattagtttggaagaagattatagaccaggaataacctacattgttgtgcagaaaaggcatcacaccagactattctgtgctgacaaaactgaaagggtgggtaagagcggcaacgtaccagcaggcactactgtggacagcacgatcacacatccttctgaatttgacttttacctctgtagccatgcgggaattcagggaaccagccggccctcccactatcaggtcttgtgggatgacaactgttttactgcagacgaactacagctgctgacgtatcagctgtgtcacacgtatgtccggtgtacgcgatctgtctctattccagctcctgcatactatgccaggctggtagcgtttagggccaggtaccatcttgtggacaaggatcatgacagtgctgaaggcagccatgtgtcaggacagagcaatggccgcgatcctcaggctctggcaaaggcagtgcagatccaccatgatactcagcatacgatgtattttgcttgagagcatctgggaagatgaggcaaaaccaacaacccatgcagtcctgaaatgttttgaacgcctactgctatggctagggcccagtttatttcagactgcccactaccagcagctctgaacagtcgcactgaatctgtacttcacagcgatgtctgatgcaccaacacaattgagccatttgtttcattattttatgtaatagaaatccatagactgtcttttctgatgcattggactgaatttttacctcaaagcgcaaacggctgatcatcctgaaatttttaaaggacttggcacgaaaggtttctattgaatattttgctagctaccatttttaagttatcttctcatcgcattagtttatcatgacgactgattccctgtcttcatgcactgcttagtgagtatgcatattatggcaaaatgttttactccagttaaatgtaattgttctattgtgtggggccatagattttttaatggagattttagacttcactactgtaaatgccttttaacaaacataactttcacaggtattgcagttttttgtcagttttactaattatctttttaatctctgcagacttcggctgcaaagcaattgcactatactgcgagttcaaataggtggttcttgttctgtgaatgtgtaatataaccagatagtgatgcctgtcagtagattttaattataatggctacaaataatactgccatggtatcgtaacaagtactgaaccatcatttccccctct

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