LOCUS       XM_068038569            4071 bp    mRNA    linear   VRT 30-AUG-2024
DEFINITION  PREDICTED: Heterodontus francisci NRDE-2, necessary for RNA
            interference, domain containing (nrde2), mRNA.
ACCESSION   XM_068038569
VERSION     XM_068038569.1
DBLINK      BioProject: PRJNA1152550
KEYWORDS    RefSeq.
SOURCE      Heterodontus francisci (horn shark)
  ORGANISM  Heterodontus francisci
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Chondrichthyes;
            Elasmobranchii; Galeomorphii; Heterodontoidea; Heterodontiformes;
            Heterodontidae; Heterodontus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_090379) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_036365525.1-RS_2024_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Heterodontus francisci"
                     /mol_type="mRNA"
                     /isolate="sHetFra1"
                     /db_xref="taxon:7792"
                     /chromosome="9"
                     /sex="female"
                     /tissue_type="heart, liver, blood"
                     /dev_stage="juvenile"
                     /geo_loc_name="USA: Port of Long Beach, California"
                     /lat_lon="33.7503 N 118.2303 W"
                     /collection_date="2021-02-21"
                     /collected_by="Ralph Appy"
     gene            1..4071
                     /gene="nrde2"
                     /note="NRDE-2, necessary for RNA interference, domain
                     containing; Derived by automated computational analysis
                     using gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 6 Proteins"
                     /db_xref="GeneID:137373585"
     CDS             33..3518
                     /gene="nrde2"
                     /codon_start=1
                     /product="nuclear exosome regulator NRDE2"
                     /protein_id="XP_067894670.1"
                     /db_xref="GeneID:137373585"
                     /translation="

MEPVSGSLFPAFDEWKVPCGSGSEGLDWLSNQSFRTEDALQLHHRIAGTAADTAQGSSQTSVCSEPRLLDRDKEDVPSKKKKKKKHRKHKKDKRKNRENHTSSDSDPEFIQDKDKEAQAVRSEASADLPQGNFMWLDDLHLQTAQTFCIDRKADPANWEYKSLYRGDLARYKRKVDTCLGLDTKKQHIIWEDSSRQKKKAKKRDDRYYSTSSLQLLSTDTVPVHCGDVLQEGIPLPGPSSFIPVPDWVENDNESSGTIAWFNPLGIYDASTTLWLQGKGQQKQEDQVQLPLDSSERINSTMMARVEKFNRMLREDPGDVQNWMKFVHFQDELMRSPNPYTATAAESEKRKRLHRVALEKKLAILERAIESNPNNADLKVARLGFCKESWEPSNLVTEWKKVVFLHPNNTALWQRYLLFCQSQFSTFSTSKVNGIYGKCLSTLAAALDGTLLSHPAQPDTEEAMLAIFLQQCHFLRQTGHSEKAIALFQALIDFTFFKPDTVAGLMTKAQVEFFEPFWDSGEPRFGEEGARGWKAWMRQQEKGGWVVLNEPGDDEEEDEDGEEVKDKSLPKWQLWMQVESTRDFKHWLPWRADKAKGQTEEDCEDPDRQVLFDDIGSSMFKISSPQLKFQLMSSFFQFLGIPAKFGLPPSCLNMSLDEPGLFSSGSSSERMLAWGESPVSGVSAVGFMTAVLDQKRTACHCKEGEDFIRTAFHQALPLFTGDERSELALSWLQYEKSKVVRNLQLRNKKQLKFQGKRSKKLAKNLLKETENRNNLALWKEYAHLEWLLGNVEDSRKVFDTAIAMAVTQGLKSSMLCDLCFLYASLELELMGSIVGSTTCRAVYILTKFAQGNAYSPYAGEVLPIHVLKARKTYEHSLQDLLTNDLTSPDPLLDKQRPGQLVSLIGCFALFQYLTVGIQAADTLYKQTSERLRGLSSLNKIQPNKSTWGHSVTSVSERLTMMHVTLLRFHMKVSVYPLGPLREILAHALRQYPANHDLWKLYVQVESKSHNASRSRRFFDTITRTAKDIILPWLFAIYAEERRKERVDSVQRADIGLVYSTLPETGLTNRIRALFEHAVQTEAGAHCVLLWRMYLNFMATQGNVERIKGVFYRALQECPWAKVLYLDAVGHLPGQLQEIVDLMTEKEIRIRVPIEELEILMED"

     misc_feature    468..770
                     /gene="nrde2"
                     /note="MTR4-interacting domain (MID) found in nuclear
                     exosome regulator NRDE2 and similar proteins; Region:
                     NRDE2_MID; cd22200"
                     /db_xref="CDD:412062"
     misc_feature    order(468..494,501..506,516..521,525..527,531..575,
                     582..590,594..602,624..632,642..647,651..656,663..683,
                     711..722,741..770)
                     /gene="nrde2"
                     /note="MTR4 binding site [polypeptide binding]; other
                     site"
                     /db_xref="CDD:412062"
     misc_feature    951..1949
                     /gene="nrde2"
                     /note="necessary for RNA interference; Region: NRDE-2;
                     pfam08424"
                     /db_xref="CDD:462472"
ORIGIN      

accgagttcggaatgagcgcggcctccacaggatggagccggtgtcaggctccttatttccggcgtttgatgagtggaaagtaccttgtggaagcggttccgaaggattggattggctcagtaatcaaagcttccgcactgaagatgccctgcagctgcaccatcgcatcgcggggactgcagcagacacggcccaggggtcatcccagacgagtgtttgttcagagcccagattgttggaccgtgacaaagaggatgtcccaagcaaaaagaagaaaaagaagaaacatcggaaacacaaaaaggataagaggaaaaatagagagaatcacaccagcagtgactctgatcctgagttcattcaagataaagacaaagaggctcaagcagtgaggtctgaagccagtgctgatcttcctcagggtaacttcatgtggctggatgacctccatctgcagactgcacagaccttctgtatagatagaaaggctgaccctgccaactgggaatacaaatcactctatcgtggagatctggcaaggtataagaggaaagtggacacctgcctcggcctcgataccaaaaagcaacacattatttgggaagattcatcccgtcagaagaaaaaggcaaagaagagagatgatcgatactacagcacatctagccttcagctcctgagcactgacacggttcctgttcactgtggagacgttttacaagaaggcattcctcttcctggtcccagctccttcataccagttccagactgggtagagaatgacaatgaatcctcaggcaccatcgcctggttcaatccgcttgggatttatgatgcttccaccactttgtggctacaaggaaaaggccaacagaagcaggaagatcaagtgcaattgcccctagacagcagtgagaggatcaattcaaccatgatggccagagtagagaagttcaacaggatgttgagagaagaccctggggatgtccaaaactggatgaagttcgttcattttcaggatgaattgatgcgaagcccaaatccatacacagccactgcagcagaatctgaaaaaaggaaaaggttacatcgagttgccttggagaagaagctggccattttggagcgagccattgaaagtaatcccaacaacgcagacctgaaagtagcccggctagggttctgcaaggaatcttgggaaccgtccaatctggtcactgagtggaaaaaagtggtctttcttcacccaaacaacaccgctctgtggcagagatacctcctgttctgccagagccagttcagcaccttttcgacgtccaaggtcaatggcatctacggcaagtgtctgagcactctagccgctgctctagatggtaccctgctatcccatcctgcacagcctgacacagaggaagctatgctggcaatttttctgcagcagtgtcatttcttacgacagactggccattcagaaaaagccattgccctcttccaggctctcattgacttcaccttcttcaaacctgacactgtcgctggcctgatgaccaaggcacaggtggaattctttgagccattctgggacagcggtgaaccccgatttggagaagaaggagcgaggggttggaaggcatggatgcggcagcaggaaaaaggcggatgggttgtcctaaatgagccaggcgatgatgaagaggaggatgaagatggagaagaagtcaaggacaagtcgctacccaagtggcagctttggatgcaggtggagagcacccgtgacttcaagcattggttgccatggcgagctgataaggcaaaaggtcagactgaagaggactgtgaggatcctgacaggcaggtgctgtttgatgatattggttcctcgatgttcaaaatctccagcccacagctaaagttccagctcatgtcctcctttttccaatttctgggaatcccagcaaagtttggcctccctccctcctgcctgaacatgtcgctggacgagcctggcctgtttagcagcggttccagcagcgagagaatgctagcatggggtgaatctccagtctcaggagtaagtgctgtcgggttcatgactgcagtgctcgatcagaagaggacagcctgtcattgcaaagaaggggaggacttcatacggacagcattccaccaagccttgcccctgtttactggggatgagcggtccgagcttgcactgagctggctgcagtatgagaaatcaaaggttgtgaggaacctgcagctcaggaacaagaaacagctgaaatttcaggggaagaggagcaagaaactagccaaaaacctcctgaaggagacagaaaacaggaataatctagctctctggaaggaatacgcccacttggaatggctactgggcaacgtggaggattcgcgaaaggtctttgacacagccattgctatggctgttacccagggcctgaagagctccatgctgtgtgacctctgcttcctctatgcctcactggaactggagttaatggggagcatcgtagggtcaacgacatgtcgagcagtttacatcctgaccaaatttgcccagggtaatgcctattcgccttatgcaggtgaagtgctgccaattcatgtgcttaaggctcgaaaaacatatgagcattctttgcaggacttgttaaccaatgacttaacttcaccagatccgttgttggacaaacagaggcctggtcagctggtaagcctcataggttgctttgcactcttccagtacctcacagtgggcattcaggctgcggacacactctacaaacagacttctgaaaggcttagaggcctctcctcactgaacaaaatacaacccaacaagagcacttggggtcacagtgtgacttctgtctctgagaggctgaccatgatgcacgtgacactgttgcggttccacatgaaagtcagcgtgtatcccttgggccctcttcgtgagatacttgcacatgctttgaggcagtatcctgccaaccacgatctctggaagctgtatgtgcaggttgaaagcaagtctcacaacgccagcaggagcagaagattctttgacaccatcaccaggactgcaaaagacataatactgccgtggctatttgcaatctatgcagaagaacggaggaaggagcgtgtagattctgtgcagagggctgacattgggcttgtgtacagcactctcccagaaaccggcctcaccaatcggatcagggcactgtttgagcacgctgttcagactgaggctggtgcgcactgtgttttactctggaggatgtacctgaactttatggctactcaaggaaatgttgagagaattaagggtgtgttttacagagcactacaggagtgtccatgggcaaaggtcctgtacctggatgcagttggccacttacctgggcagctccaagaaatcgtggacttaatgactgaaaaggagatccggatccgggttcctatcgaggagctggaaattctcatggaagattgattagggaagaaagaagacttgcatttacacagcacctttcacaacattgggacatcccaaagcacttgacagccaattagtcagtgttataaacctagggaaatgaggcagtcagtttgtgcactgccaagctcccacaatcagcagtagtgagataaatgatgtgataatgtattggctgagggatgaatgttggccaggacagctgttatgcaggcaaatatggcaacactttggaacgcagcaagatcccgcccctccaaaaagtgcagcaattaactcggtgataccggttgagtgggcaataccaccagccgggatatcgagagaacaacctgctcttcaaatggtgccatgagatctttgacatccaccaaaaccgactcgatggggcttggtttaacattccatccgacggatgaatcattgaagcattcagtagtaggatttaaagaaaagcagaatatttattttgtaataaataataaacatgtacagtttgtttatttgtatttttactgtttgctaagctggagattaaaaattactgagt

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