LOCUS       XM_066717147            3235 bp    mRNA    linear   VRT 30-SEP-2025
DEFINITION  PREDICTED: Amia ocellicauda protein argonaute-1 (LOC136763271),
            transcript variant X2, mRNA.
ACCESSION   XM_066717147
VERSION     XM_066717147.1
DBLINK      BioProject: PRJNA1136883
KEYWORDS    RefSeq.
SOURCE      Amia ocellicauda
  ORGANISM  Amia ocellicauda
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Holostei; Amiiformes; Amiidae; Amia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_089860) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_036373705.1-RS_2025_09
            Annotation Pipeline         :: NCBI Eukaryotic Genome Annotation
                                           Pipeline (EGAP)
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 09/26/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Amia ocellicauda"
                     /mol_type="mRNA"
                     /isolate="fAmiCal2"
                     /db_xref="taxon:2972642"
                     /chromosome="11"
                     /sex="female"
                     /tissue_type="blood, heart, muscle, misc"
                     /dev_stage="adult"
                     /geo_loc_name="USA: Vacherie, Louisiana"
                     /lat_lon="29.929889 N 90.747444 W"
                     /collection_date="2023-03-15"
                     /collected_by="Ingo Braasch, Allyse Ferrara"
     gene            1..3235
                     /gene="LOC136763271"
                     /note="protein argonaute-1; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 3
                     Proteins"
                     /db_xref="GeneID:136763271"
     CDS             247..2331
                     /gene="LOC136763271"
                     /codon_start=1
                     /product="protein argonaute-1 isoform X2"
                     /protein_id="XP_066573244.1"
                     /db_xref="GeneID:136763271"
                     /translation="

MRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIAEQPKTLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMAEVTGRVLPAPILQYGGRSRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEVIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKAERIGKSGNIPAGTTVDTSITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA"

     misc_feature    277..429
                     /gene="LOC136763271"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    430..792
                     /gene="LOC136763271"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(586..588,631..633,673..675,685..687,739..741,
                     760..762,766..768)
                     /gene="LOC136763271"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    925..2202
                     /gene="LOC136763271"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1336..1338,1348..1350,1384..1395,1402..1404,
                     1426..1428,1435..1437,1447..1449,1459..1461)
                     /gene="LOC136763271"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1540..1542,1546..1548,1756..1758,2170..2172)
                     /gene="LOC136763271"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

ctactttgaggtggagatccccaagatggatgtctatcactacgaggtggacatcaaacctgacaagtgcccacgccgtgtcaacaggtggattttgaggtaactattccaggtgaggggaaggacaggatcttcaaggtctctatccgctggctggctgtggtgagctggcggcttctgcaggaggcgctggtgagcggccgggtacaggtccctctggactcggtgcaggcgttggatgtcgccatgaggcatctggcctctatgaggtacactccagttggtcgttccttcttctctccccctgaggggtactatcaccctctggggggcgggcgggaggtgtggttcggtttccaccagtccgtgaggccggccatgtggaagatgatgctgaacattgacgtgtcggccactgcattctacaaggcccagccggtcatcgagttcatgtgtgaggtgctggacatccgcaacatcgccgagcagcccaagacactgacggactcacagagagtccgcttcaccaaggagatcaaaggtctgaaggtggaggtgacgcactgtggccagatgaagaggaaataccgcgtctgcaacgttacccgccgccccgccagccaccagacgtttcccctgcagttggagagtgggcagacggtggagtgcacagtggcacagtacttcaaacagaagtacaacctgcagctcaagtaccctcacctgccctgcctccaggtggggcaggagcagaagcacacttacctgcccctggaggtgtgtaacatcgtggcggggcagcgctgcatcaagaagctgacagataatcagacctccaccatgatcaaagccacagcacgctctgcccctgacaggcaagaggagatcagccggctgatgaagaatgccagttataacctggacccctacatccaggagtttggcatcaaggtgaaggacgacatggcggaggtgacaggcagggtgctgccagcgcccatcctgcagtatggcggccggagccgggccatcgccacgccgaaccagggtgtgtgggatatgcgggggaagcagttctacaacggcatcgagatcaaggtgtgggccatcgcctgcttcgccccacagaaacagtgcagagaggaggtgctgaagaacttcacagaccagctgcgcaagatctcgaaggacgcggggatgcccatccagggccagccgtgcttctgtaaatacgcacagggcgctgacagtgtggagcccatgttccgccacttgaagaacacttattcagggctgcagctcatcattgtcatcctgccgggcaagacccccgtctacgcggaggtgaagcgtgtgggagacactctcctgggaatggccactcagtgtgtccaggtgaagaatgtggtgaagacgtccccccagacgctgtccaacctctgcctcaaaatcaatgtcaagctgggcggcatcaacaacatcttggtaccacaccaacgctccgcggtgttccagcagccggtgatcttcctgggcgcagatgtgacacacccccctgcaggcgatgggaagaagccgtccatcacggcggtggtgggcagtatggacgcccaccccagccggtactgtgccacagtgcgtgtgcagcgcccaaggcaggaggtcattgaggatctctcttacatggttcgtgagctactgatccagttctacaagtcgacccgcttcaagcccacccgtatcatcttctacagggacggggtgcctgagggccagctgccacagattctgcactatgagctgctggcaatccgggatgcctgcatcaagctggagaaggactaccaaccaggcatcacctacatcgtggtgcagaagcgccaccacactcggctgttctgcgccgacaaagcagagcgaattgggaagagtgggaacatccctgctggcaccacagtcgacaccagcatcactcaccccttcgagtttgacttctacctgtgcagccacgccggcatacagggcaccagtcgtccctctcactactatgtactgtgggatgataatcgtttcactgccgacgagctgcagatcctcacctatcagctgtgtcacacgtatgtgcgctgcacacgctctgtctccatccccgcgcccgcctactatgcccgactggtggcgttccgtgcccggtaccacctggtggacaaggagcatgacagtggggagggcagccacatctctgggcagagtaacggccgggacccccaggccctggcaaaggccgtgcaggtgcaccaggacactctgcgcaccatgtacttcgcctgagagacctccgccgccctcctacctcaacaaactcactgagccagccccccacttccgtctcctggagcagacggacctatggatgtatagatagacagacagacacactccgctccacacagcagggcactgtttttatgactccaggattatatattttttatatatataatgacatatatctatgcgtctatatatttacactcacaagtatgtatgcaataccgaagggaaggaactgaggtctctgaccagccaattgctgcctctgtttgcagacatgcccccgcagcctgccaatcacatcacagatgcagcaccttggacattttatctgttgtgtctatttctactgcgaaaagaataacagacaacaattgaaacacttatgattttattcttgttcttcatatttgttgttttatatttttatatgtttgtttttttacccttgaggcaaggtacaatagcacttatagagttaggcactacctgtgttagctgtgagcagtagactagagtgcagtaggtatggagagtgccttactgtagtgacacaggacagctgaagccagagacactggtcgagggtaagagtgaggagccaccctctacactgaccttgagagagagagaaagagagcagattgggctgtgagagagagagaggggagccaccctctacattgaccctgaaaagaccgctgctacagttcttccctgtgccccaatccagtccagcccgctacagtgcggtcctgttggtgcccacggactgactgactggcagtgtggaggcatcactcaatcacagcaaatccacacagaaccgactctgggtctcaaagctgtatccaatagtgtgaaatctctggttattttttaaataaaatttgtgtttttc

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