LOCUS       XM_066083736            5968 bp    mRNA    linear   INV 28-JUN-2024
DEFINITION  PREDICTED: Magallana gigas protein argonaute-2 (LOC105321320),
            transcript variant X2, mRNA.
ACCESSION   XM_066083736
VERSION     XM_066083736.1
DBLINK      BioProject: PRJNA1118722
KEYWORDS    RefSeq.
SOURCE      Magallana gigas (Pacific oyster)
  ORGANISM  Magallana gigas
            Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Bivalvia;
            Autobranchia; Pteriomorphia; Ostreida; Ostreoidea; Ostreidae;
            Magallana.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_088857) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_963853765.1-RS_2024_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/20/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Magallana gigas"
                     /mol_type="mRNA"
                     /db_xref="taxon:29159"
                     /chromosome="5"
     gene            1..5968
                     /gene="LOC105321320"
                     /note="protein argonaute-2; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 6
                     ESTs, 16 Proteins"
                     /db_xref="GeneID:105321320"
     CDS             125..2860
                     /gene="LOC105321320"
                     /codon_start=1
                     /product="protein argonaute-2 isoform X2"
                     /protein_id="XP_065939808.1"
                     /db_xref="GeneID:105321320"
                     /translation="

MFPIAPGIAGLVTQQLNGASHGGCTDLQPPNGILSADIRSTFGPFTTVPPPEPPPHPADQPPPTPPPPSNGNAGEFICPLRPDHGREGKPIALRANHFHVNIPKGFIHHYNVAITPDKCPRRVNREIVETMVNAYTPRIFSGQKPVFDGREKLYSREPLPFGKDKVELEVTLPGEGRDRVFKVAIKWLSQISLYALEEALEGRARRIPECAVEALDVIMRHLPSMMYTPVGRSFFSPPEDYDYPLGGGREVWFGFHQSVRPSHWKMMLNIDVSATAFYKAQPVIDFMCEILELKDANEQRRPLTDSQRVKFTKEIRNLKVEITHCGTMRRKYRVCNVTRRPAQTQSFPLQLDSGQTVDCTVARYFLERYKMKLQHPHLPCLQVGQEHKHTYLPLEVCNVVGGQRCIKKLTDLQTATMIRATAKNAPDREKEINNLVKKASYNNDPHLRTFGITVNPQMMDLHGRVLSHPKLQYGGTTKAQALPNQGVWDMRGKQFYFGIEIRVWAIACFAPQRSVREDALRNFTQQLQKISTDAGMPILGQPCFCKYATGPDQVEPMFRYLKNTYAGLQLIVVVLPGRTPVYAEVKRVGDILFGLATQCVQSKNVNKTSPQTLSNLCLKINVKLGGINNILLPSSRPLVFREPVIFLGADVTHPPAGDTSKPSIAAVVGSMDAHPSRYSATVRVQEHRKEVIEEFCSMVRELLISFYKSTQFKPTRIIIYRDGVSEGQFQKVLAHELRAVREACMKLEIGYQPGITFIAVQKRHHTRLFCADRKDQIGRSGNIPAGTIVDVGITHPTQFDFYLCSHAGIQGTSRPSHYHVLWDDNCFKADELQQLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVEKEHDSGEGSRHSDEMERTPSAMARAITVHPDTIKVMYFA"

     misc_feature    389..781
                     /gene="LOC105321320"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    809..961
                     /gene="LOC105321320"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    962..1324
                     /gene="LOC105321320"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1118..1120,1163..1165,1205..1207,1217..1219,
                     1271..1273,1292..1294,1298..1300)
                     /gene="LOC105321320"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1457..2734
                     /gene="LOC105321320"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1868..1870,1880..1882,1916..1927,1934..1936,
                     1958..1960,1967..1969,1979..1981,1991..1993)
                     /gene="LOC105321320"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2072..2074,2078..2080,2288..2290,2702..2704)
                     /gene="LOC105321320"
                     /note="active site"
                     /db_xref="CDD:240015"
     polyA_site      5968
                     /gene="LOC105321320"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

atttttttgggtgattagtataacgtcaatcgtgcgccatattttcttttgacaaaagatgaaaatgacagaagtttgtttacctctcctgatttaccattttccctcgtcataagatgtcataatgtttcctattgcaccaggtatagccggcttagtcacccagcaactgaatggggcaagtcatggtgggtgtactgacctgcagccacctaacggaatcctatcggcagacatacggtccacctttggtccttttaccacagtccccccaccagaacctccaccccacccagctgatcagccaccccccacccctccacctccatctaatgggaatgccggggagtttatctgccccctcaggcctgaccatggacgggagggaaaacccattgctctacgggccaaccattttcatgtcaacatccctaaagggttcatccaccactacaacgtcgccattacacctgacaaatgtccccgacgagtcaacagagagatcgtggagaccatggttaatgcctacacaccccggatcttctccggacagaagcctgtgtttgatggcagagagaaactgtacagtcgagaaccacttccattcggcaaagataaagtggagttagaggtcaccctgccaggtgaaggtcgtgaccgggtgttcaaggtcgccattaagtggctgtcccagatcagtctatacgccctggaggaggcgttagaggggcgtgcccggaggattccagagtgtgcggtggaggcgctggacgtcatcatgaggcacctccccagcatgatgtacacccctgtgggtcgttcattcttctctcccccggaggactatgactaccccctgggagggggcagggaggtgtggttcggcttccatcagagcgtccgtccctcccactggaaaatgatgctcaacattgatgtatcggccacagccttctataaagctcaaccggtcatcgacttcatgtgtgaaatcttggagctgaaggacgccaacgaacagcgacgcccactgaccgattcccagcgggtcaagttcaccaaggaaatcagaaacctgaaggtagaaatcacccactgtggaaccatgaggaggaaatacagagtgtgtaacgtcactcgcagacccgcacaaactcagtcatttccgctacagctggattcgggtcagacagtggattgtacggtcgctcggtacttcctggagcgctacaaaatgaagctacaacaccctcacctgccgtgtttacaagttggacaggaacataagcacacatatcttccattagaggtttgtaatgttgtgggaggccagagatgtatcaagaagctgacagacctacagacggccaccatgatcagggcgacagccaagaacgcccccgacagagagaaggagatcaacaatctggtcaagaaggccagttataacaatgacccccacctgaggacgtttggaatcacagtaaacccccagatgatggatctacacggccgggtcctctctcaccccaaactacagtacgggggaacgacgaaagcccaggccctgccgaaccagggcgtgtgggatatgcgagggaagcagttctattttgggatcgagatccgtgtgtgggccatagcatgcttcgccccccagaggagcgtgagggaggatgcattgaggaactttacccagcagttacagaagatttctacagatgcaggcatgcccattcttggtcagccgtgtttctgtaagtatgccactgggcctgaccaagtggagcccatgttccgttacctgaagaatacgtatgcagggctacaactgattgtggtggtcttacctggacggactccagtgtatgcggaggtgaagcgtgtgggagacatcctgtttggtctggccactcagtgtgtacagtccaagaacgtgaacaagacctcgccccagacgctatctaacctgtgtctgaagatcaacgtcaagctgggcggcatcaacaacatcctgctacccagctccagacccctggtgttccgtgagccggtcatctttctgggagctgatgtgacccaccccccagctggagataccagcaaaccctccatagccgccgtggtggggagtatggacgcccaccccagccgttactcagcgacggtgagggtacaggagcaccgtaaggaggtgatagaagagttctgctccatggtcagggaactcctgatctccttctacaagtccacccagttcaaaccgacccgcatcatcatctacagggacggggtcagcgaaggacagttccaaaaggtgcttgcccatgaactgagagcggtgagggaggcctgtatgaagctagagattggttaccagccgggaatcaccttcattgccgtccagaagaggcaccacaccaggctcttctgtgccgaccgcaaggaccagataggacgtagcgggaacatcccggcgggaaccattgtggatgtcggaatcacccaccccacacagtttgacttttacctatgcagccatgccggtatacagggcaccagcagaccctcccattaccacgtgttgtgggacgacaattgtttcaaggcggacgagctccagcagttgacataccagctgtgtcacacgtacgtacgctgtacgcgtagcgtgtccatccctgctcctgcgtactacgctcacctggtggcgtttagagcgaggtatcaccttgtggagaaagagcatgacagcggagaagggagccgtcactcagatgagatggagaggacaccctcggccatggctcgagccatcaccgtccaccccgacaccatcaaagttatgtacttcgcttagaccacgaaacaattacctgatcttaggaaatgatttaactcaccagatattggcaacctgccaaaaaccagctcacccaaactttacaaagtgctcccacggaacagtctagtcatccaccacagcgtcgacgattcatgtttattcatgtgtacgacgatgtcttatcgcacatttctacttttatttttgttaataaactgtccctatcaatgcatgagtgagcaattgttaaatataaatgtatttgttttttttgaaaaaatgatatgaataatattagagtgtaattactagagaacactattaatttggaacagtgtatttttctagaattgtgaagttttgtccattttgagttggttttcttacttgtagcaaatttttgaactggtggtgattatttgaggccatcatactatttttcaaaatgttttccattgacttatcggggtcaagtgctgttttttggagatggtttataggggtttggtgtctgatgcatgcttgttatggtctctgaacacctgtcagtgttgacacttgatatttaaagggtgttgtatatgtagtcttgaacaacatgatcttttttttaaaaatgatcattcgaacaataaggtgtatctttttgtattttgtaaagaattcattaatgcatgatttttcgcaataagtgtgatgttacattgggtgttgggaatgtttcagtccgagttttgcatctggggaaattacaatgcatattcaagaactgtgaaatattgacaacaattagttcatctttcaaactgaatctcataatttcaaggtcaaagtaccaaaatttatcaatattttgatcccattggagtcttcataatagagttagagcagctgttcaatattaagattatactttttatacaccatatttgatcctctatgtaacacattttaacaccggactgagtgtataaagatgttatatatattagaattgttcatatcatgtcattttgtgtgatgtacactgttttaccaagcagaagccgggaatctgttattatgtatttacactgcatcatttcaagataagttcatgttagaatggcagtagaatttttgtctattttaatcttacactcagtatatagtcttgttattcgctgtagaatcattcaatttgtgggctgtaattttcatggattgcttgttccaagaatgtagtcaaacattaattttatgaaattctttaaaattaatttggttgttcctatccacagaatccatgaattctggattaagagttccacaacattcatggatttgtggattttggaacccacactattgataaatttgtaaataaatgtatccagaagtaaatgaactttgaccccacaaaagtaatgagaccacagtactagcattggatagtcacagactagataaattgtaccctgtatcttaattaaatgattccttgtaattcttcctttgattagccccagaatcaatgtaaataatactaaagtatgttcaattgtgggtgttattgtcaaacatttgatgtcagtactccatcatagcaggacgtgacaaggttaggtcaaacagatcatgacccagtgtttacacctgtacaggtaatggaatagtgtgaaagtaccttccacatctggccagtagttgagagaaggtcaaggtcaggctatactggagtcaaggtcacaaaccaaatgatggcgttttctttgtatgtgcatatataaagcaatggaagaaaggctgtggacaacttttactttgtactgtattattctgtggtgtctctgtagtcagttttacttttagttatttacatcatgtgcagtgaactttgaactcttgttgttataaacaatatattttgttatgataaaaaatttcaaagcaaaacaaaaaaaatacatttctgaccttgtgtaatgcattgataaggaaaagtccttgcagcaattctgaatgatagaattatgtagacatggccctatgaagttaataatggggatgatgatagaattatcccattcactgtgtacacatgtatgctcaacatagtgctcccaaacagctaactatgcaggtccattatacgtatacagagaagctatggacatgtctacttcacgtaacacaatgaatatgggcaggttcattacttatctcacaatactgacaaaagctatgggcaggtccattacacataacagaaaaactatgggcaagtccattacacaactatggacaaatccattacacattttagaaaaccttttggaaggtcagatgctaattaccataactatgggcaggtcctcttcacatagcagaaaccattggcaggtccattacccaaaaaaatgtaggggcaggcccattacaccacacaggaaaaattatgggcatgtcatcacagaaaaaatagctatgagcagacacactgcacatcatggagaaactatgagcaggtccattacacatcatggagaaactatgagcaaagccatatgaatatggattttaaacatggctcacagtgactagaaattgatttattacatcatttcacaacaaactttatcttaaggttcataaagtgtatgggcagctaattgttaataaataagtactttactagggaaactctcatgttattcatctgatgaaaattgcacaacagcagtttttgtgaatgctgttaagaattggaagtattgcaactgactgttaacacaacaaatgtaaaattttgatggcacaatattatgaaatttattaaaacttgatctttctgtaaaactatgtacagtgcttctcagttaatgtttctgttgtgtaaggatgtcatattattattcgacaaatgagataatcatgttataattttggatcatcggactttgagatcacagtggtttatagaatgaatatcatttttagaaatttacaaatgcatgaactttattcatatcctatatctatattgaatatgttgtcctttatctgttaacttctttgtttttatctgttataattgtagatttacagcttaaatttaacaaaataaattgtctttgatacaga

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