LOCUS       XM_060756814            3677 bp    mRNA    linear   VRT 20-AUG-2024
DEFINITION  PREDICTED: Anolis sagrei NRDE-2, necessary for RNA interference,
            domain containing (NRDE2), transcript variant X1, mRNA.
ACCESSION   XM_060756814
VERSION     XM_060756814.2
DBLINK      BioProject: PRJNA1142516
KEYWORDS    RefSeq.
SOURCE      Anolis sagrei (Brown anole)
  ORGANISM  Anolis sagrei
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata;
            Toxicofera; Iguania; Dactyloidae; Anolis.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_090021) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Aug 20, 2024 this sequence version replaced XM_060756814.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_037176765.1-RS_2024_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.3
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/09/2024
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Anolis sagrei"
                     /mol_type="mRNA"
                     /isolate="rAnoSag1"
                     /db_xref="taxon:38937"
                     /chromosome="1"
                     /sex="male"
                     /tissue_type="blood, liver"
                     /dev_stage="subadult"
                     /geo_loc_name="USA: Palm Coast, Florida"
                     /lat_lon="29.582251 N 81.214912 W"
                     /collection_date="2019-09-07"
                     /collected_by="Tonia Schwartz"
     gene            1..3677
                     /gene="NRDE2"
                     /note="NRDE-2, necessary for RNA interference, domain
                     containing; Derived by automated computational analysis
                     using gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 8 Proteins"
                     /db_xref="GeneID:132763384"
     CDS             77..3550
                     /gene="NRDE2"
                     /codon_start=1
                     /product="nuclear exosome regulator NRDE2 isoform X1"
                     /protein_id="XP_060612797.2"
                     /db_xref="GeneID:132763384"
                     /translation="

MALFPAFAGAAKPGEAPTGESGGSKREFNWLTNPSFSSEDALRLHQHVLEVTDPDAEKLSVQRTTSSLEPPAESEACESSEKTAKKRKKKKKREHKKVKKRKRSRDQRTSDSESDIEDVKDKNNEKRGAELNLGDKTASVLCSSIWLDDIQGLGTELFRTDKKPDPANWQYKSLYRGNIARYKRRSNLCLGIDLKRQHIIWESSATEKKSSCKRTERYFTKGGLKLLKVDGIPITSQSQNSTPGSATWIPVLQRDTDDIPTTSYVNPLGIYDPLTTLWLQGKGPPEDDSLKQQENQEKSESGTSHIMTKLEEHNRMVRENPRDVKAWMDFVSFQDELMRGPSCFTMSEGERETRKKSLKLVLEKKLAILERAIENNPNSVDLKLARLKLCMEFWEPTAVIKEWQKLVFLHPNDPLLWQKYLLFCQSQFSTFVVSKVHSLYGKCLSTLSAVQDGSMVSHPALPGTEEAMLAIFLQQCHFLRQAGHSEKAVALFQALIDFTFFKPDSVKELLTRGQVEFFEPFWDSGEARIGEKGAKGWRAWMHQQEKGGWIVLNPPDEDEEDAEEEEEIQNKSLPKWQNWLHIERSREARHWLPWRPDKTKKETVDDCEDPERQVLFDDLGPSLIKISNPDLHFQLLCSFLQFLGVPCSNSLLPSFLYLALDENSIFDKGQNSQGPLTSFDLPLLGVSCIGQMDSVAQGRNHVGHPKEGEEFIQNFFHMMLPLFSGKKRSDLSVFWLQYEFCKVIWCLKTRNKKKLKLQGKKSKKLAKNLLKAPDNRNNISLWQLYAHLEWLLGNTEEARKVFDMALSMAGEAGVRTLQVCKLGLLYAELETELLGAPEGSLECRAVHILTSLVEKSPYVPYTGQVLSVNILKARKTYQHLLQEYLSEGSVSEQEQTSFSSHLAGVVGCYSLFQYLTLGIDAAVSIYREVHEKLKGKKPSEGLSSQNFTPALESVTLMHVSLLRYHMKTTVYPLHPLREALLEALKMYPSNQHLWRAYVQIQSKSHNASKARRFFDSITRTTKSLEPWLFAIQVEQMRKKLVDSVQRGANGDVHATIPETGLTNRIKALFEHAIETENGAHCPLLWRLYIDFMVSLGDKEKSKGLFYRALQNCPWTKVLYMDAIEYFPDELQETLDLMAEKELRVRLPIEELELLLED"

     misc_feature    548..829
                     /gene="NRDE2"
                     /note="MTR4-interacting domain (MID) found in nuclear
                     exosome regulator NRDE2 and similar proteins; Region:
                     NRDE2_MID; cd22200"
                     /db_xref="CDD:412062"
     misc_feature    order(548..571,578..583,593..598,602..604,608..652,
                     659..667,671..679,701..709,719..724,728..733,740..760,
                     776..787,806..829)
                     /gene="NRDE2"
                     /note="MTR4 binding site [polypeptide binding]; other
                     site"
                     /db_xref="CDD:412062"
     misc_feature    1010..2008
                     /gene="NRDE2"
                     /note="necessary for RNA interference; Region: NRDE-2;
                     pfam08424"
                     /db_xref="CDD:462472"
ORIGIN      

tccattagacgcgctttctgtatcgtaggggggcgctgaggaagtggcggctcttcggggccagtgattggccgccatggcccttttccctgcgtttgcgggcgctgctaagcccggggaggctccaacgggcgagagcgggggctccaagagagaattcaactggctaaccaacccaagtttctccagtgaagatgcactaagattgcatcaacatgttttagaagtaacagatcctgatgcggagaaattatcagtccaaagaacaacgtccagtttagagccaccagcagaaagtgaagcttgtgaaagttctgaaaaaacagctaagaaaaggaagaaaaagaaaaaacgagaacacaaaaaagtaaaaaagagaaaaagaagcagggatcagagaaccagtgattcagaatcggacattgaggatgtgaaagataaaaataatgaaaaaagaggagcagaattgaacctgggggacaaaactgcaagtgtgctctgcagctctatttggctggatgatattcagggtttgggtacagaattattcagaacagataagaaaccagatcctgccaattggcaatacaagtcactgtaccgagggaacattgcaagatacaagaggagaagtaacctctgccttggcattgatctgaagagacaacatataatttgggagagctcagcaacagagaaaaagtcctcttgtaagaggacagagcgttacttcacgaaaggtggcctcaagctcctgaaggtggatggcattcctatcactagtcaaagtcaaaactctactcctggttcagctacttggataccagttttgcaaagggacacagatgatattcctactacaagctatgtgaatcctcttgggatctatgatccgctgaccactttatggctacaaggaaaaggcccgcctgaggatgactcactaaagcagcaggaaaatcaagaaaagtctgagagtggaacttctcacataatgacaaaactggaagagcacaacaggatggttagagaaaatcccagagatgtcaaagcatggatggatttcgtttcgtttcaggatgaactcatgagaggtcccagctgttttacaatgagtgaaggagaacgggagacaagaaagaagtcactcaaactagtcctggagaagaagcttgcaattctggagagagccattgaaaacaacccaaacagtgttgacctgaaactggccaggttgaagctttgcatggaattttgggagcctacagctgtgataaaagaatggcagaaattggttttcttgcacccgaacgacccactgctgtggcagaaatatctcttgttctgccagagtcagttcagcacctttgtggtttcaaaggttcatagtttgtacggcaaatgcctgagcactttgtcggcggtccaagatggcagtatggtgtcacacccagcattgccaggcacagaggaagccatgctagctatctttctccagcagtgccattttcttaggcaagctggccattcagagaaagcagtagctttgttccaggccctaattgacttcacattttttaagccagatagtgtcaaggagttgctgacaaggggacaggtggagttctttgaaccattttgggacagtggtgaggctcgtattggagaaaagggagcaaaaggttggagggcctggatgcatcagcaagaaaagggtggctggattgttctcaacccacctgatgaagatgaggaagatgcagaggaggaggaagaaatacagaacaaatctttacccaagtggcaaaattggcttcatattgaacgttctcgtgaagcaaggcactggttgccatggcggcctgacaaaaccaagaaagaaacagtagacgattgtgaagatccagagagacaggtcctctttgatgaccttggtccatctttgataaagatttccaacccagatctccactttcaactactttgctcatttttgcaattcttaggagtcccttgtagcaatagcctccttccttctttcctctacttagcgctggatgaaaacagcatatttgacaaaggacagaacagccaaggacctctgacttcatttgacttgccacttttgggggtcagttgcattggtcagatggactcagtggctcaaggcagaaaccacgtgggccaccctaaggaaggggaggagttcatacagaatttctttcatatgatgctgcctctattttcagggaagaagagatctgacctgtctgttttttggcttcagtatgaattttgtaaggtcatctggtgtttgaaaaccagaaacaaaaagaaactaaaattgcaaggcaagaaaagtaagaaattagccaagaatctcctgaaagcacccgacaacagaaacaacatttctctttggcaactgtatgcccacttggagtggctccttggcaatacagaagaggccagaaaggtttttgatatggccctgagcatggcaggagaggctggagtcaggactctccaggtgtgtaagcttggtctgctttacgctgaactggaaacagagttgcttggcgctcctgaaggaagccttgagtgccgtgcagtccatatattaaccagtctggtggaaaagagcccttatgtaccttacactgggcaggtactatccgtgaacattttgaaagctcggaagacataccagcacttgctccaggagtacctaagcgaaggctcagtctctgaacaagagcagaccagcttttccagtcacttggctggcgtggttggctgttactcccttttccagtatttaactcttgggattgatgctgctgtgtcgatatacagagaggtgcatgaaaagctcaagggcaagaagcctagtgaagggctcagtagtcaaaatttcactccggctcttgaatccgtcacattgatgcatgtgagtttgctcagataccacatgaaaacgaccgtctatcctttgcatcccttgcgtgaagctctgttggaggctttgaagatgtatcctagcaaccagcatctctggagggcatacgttcagatccagagcaaatctcacaatgccagtaaagcgcggcggttttttgacagcatcacgcggacgacgaagtcactggagccctggctgtttgccatccaagtcgagcagatgagaaaaaagcttgttgacagcgtccagaggggggccaatggagatgtccatgcaactatccctgaaactggtctaaccaaccgaatcaaagctttgtttgaacatgcaattgagactgaaaatggggcccactgcccattactttggaggctctatatagactttatggtttctttgggtgataaagaaaaaagtaaaggactgttttatcgagcgcttcaaaactgtccctggacaaaggtactatatatggatgcaatagagtattttccagatgagctccaagagacacttgatctgatggctgagaaagagttgagagtacggctcccaatagaagaactggagttgctgcttgaagactgaagaataccctgaagatgcaaaattggacatttggcccagtcctcagtatatctggtttaggatattctacatgtttgatggacaagaaattgtgctttttttggtcttcatcaaagtatatataatc

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