LOCUS       XM_045424736            4987 bp    mRNA    linear   PLN 19-APR-2022
DEFINITION  Blastomyces dermatitidis ER-3 RNA interference and silencing
            protein (BDCG_08965), mRNA.
ACCESSION   XM_045424736
VERSION     XM_045424736.1
DBLINK      BioProject: PRJNA319322
            BioSample: SAMN02953741
KEYWORDS    RefSeq.
SOURCE      Blastomyces dermatitidis ER-3 (Ajellomyces dermatitidis ER-3)
  ORGANISM  Blastomyces dermatitidis ER-3
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Eurotiomycetes; Eurotiomycetidae; Onygenales; Ajellomycetaceae;
            Blastomyces.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Champion,M., Cuomo,C., Ma,L.-J., Henn,M.R., Klein,B., Goldman,B.,
            Young,S., Kodira,C.D., Zeng,Q., Koehrsen,M., Alvarado,L.,
            Berlin,A.M., Heiman,D.I., Hepburn,T.A., Saif,S., Shea,T.D.,
            Shenoy,N., Sykes,S., Galagan,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, Broad Institute
            Microbial Sequencing Center.
  TITLE     The Genome Sequence of Blastomyces dermatitidis strain ER-3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4987)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2022) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 4987)
  AUTHORS   Champion,M., Cuomo,C., Ma,L.-J., Henn,M.R., Klein,B., Goldman,B.,
            Young,S., Kodira,C.D., Zeng,Q., Koehrsen,M., Alvarado,L.,
            Berlin,A.M., Heiman,D.I., Hepburn,T.A., Saif,S., Shea,T.D.,
            Shenoy,N., Sykes,S., Galagan,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, Broad Institute
            Microbial Sequencing Center.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2009) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. This record is derived from an annotated genomic
            sequence (NW_025544786).
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Blastomyces dermatitidis ER-3"
                     /mol_type="mRNA"
                     /strain="ER-3"
                     /db_xref="taxon:559297"
                     /chromosome="Unknown"
     gene            1..4987
                     /locus_tag="BDCG_08965"
                     /db_xref="GeneID:69030443"
     CDS             303..3431
                     /locus_tag="BDCG_08965"
                     /codon_start=1
                     /product="RNA interference and silencing protein"
                     /protein_id="XP_045273388.1"
                     /db_xref="GeneID:69030443"
                     /translation="

MSGQQPRGQRGRGGGRGVGGDPGGQRGGQRGRGSDRGRGPHPIFDAPSGGRGRGDRGGFRGDRGGRGDRGGFRGGRGGGTRGPEVYSQASGGIPAPDPKVKKVENSYKGPAGSGKELSLESLSINRNFPLRPAFGTQGRPVLLWANYFEIVSSFNLTLHRYKVEVQEQDGKTPAGKKLKRIIELLLEEHFGNMKNQIATDYKATLVCKTAINLPQEMFSITYRLEYEDEPAPRARTYKVRVVAIGSLDVAPLLQYLSSPRSGEQLPAKEEIIQALNIVLGHNPKTNRELLSIGANKHFPLGRGGESYDLSGGLTALKGFFVSVRAATSRLLVNVQVKATPCYSEGSLPDIMNSFSASIRGPTHFHKLNRFLKRVRVSVNHIVRKNKSGRVILRIKTIEGLAHQSDGRNLQNPPQVPWLGAGPKDVKFFPDGPTPPAEGQSKKNGKKQKKGPVKPGGDSSTGSYISVYDYFKRNYPNIPELNNALPVVNVGSKEEPIYLPAEVCHVLPGQPANAKISPQQTQNMIRFAVRRPVENAKSIVVNGTQVLGFTPHLNPMLAGMGLSLLPNLITVPGRVLEGPAIIQYKGQSFKSPHGGNWNLQKVAFSHGSQLPAWTFLYYQDGRTDPAVVMESVDRFIDMARVQGLAVPVPSRPIAVNVPPRRDVEDIPLDPIFAEIRQQSRVRLVLVILPFASPQIYSQVKYRGDIKDGIHTICVVAEKFRKNQPQYFANVALKFNLKLGGVNHKLQPSKLGVISEGKTMVVGIDVTHPAPGSLSTAPSIAGMVASVDKVLGQWPATIRLQQQARAEMVSDLDIMLQSRLRLWQKNNSKSLPENILIYRDGVSEGQYGKVLDEELPLLRSACKAIYPADKTKKGLPKFTIIIVGKRHNTRFYPTDLKDADNNSNPNSGTVVDRGVTESRHWDFFLQAHTALQGTARPAHYYVILDEIFTGRKTAGRFDSIADELEDLTHNLCYLFGRATKAVSVCPPAYYADLVCERARRYLNRYFDVTPDTSVASSGASDVGPVPIGDDVRVHSDLVDTMFYI"

     misc_feature    693..3425
                     /locus_tag="BDCG_08965"
                     /note="protein argonaute; Provisional; Region: PLN03202"
                     /db_xref="CDD:215631"
     misc_feature    1962..3302
                     /locus_tag="BDCG_08965"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(2385..2387,2397..2399,2433..2444,2451..2453,
                     2475..2477,2484..2486,2496..2498,2508..2510)
                     /locus_tag="BDCG_08965"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2589..2591,2595..2597,2814..2816,3270..3272)
                     /locus_tag="BDCG_08965"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

agaacatatgggatcacgtgcaggctgagcgatccctacggcaacgccactggagccgctacagttagttggttacaccccaacaaaaaaacaaacaaaacatgccaagaatggcaaagagacagttggcaacgagttacataccctgcaaacttagcgctgggattgaggcctccagcaggggtcccgtcattcactggagcctcatctcaaatccacggctggtctgaaggatatacagtagtcagcttaatcccgattttttttgattgtatcattcttttccctaaccaagcagccaaatgtcaggacagcagcctcgaggtcagagagggcggggcggagggaggggagtggggggagaccccgggggtcagcgaggtggacaacgtggacgcggctctgaccgtggccgaggtccacatcctatcttcgatgcgccatcgggcggacgagggcgtggtgaccgtggcgggtttaggggagatcggggcggccgtggtgatcgtggtgggtttcgcggaggacgaggaggcggaactcgtgggccagaggtgtattcccaagctagcgggggtattcctgctccggatcccaaggtcaaaaaggtcgagaatagctataaaggacctgctggctccggaaaggaattgagtttagaatctctttcaattaacaggaatttccctctaagaccagccttcgggacacaaggaagaccagtcttactgtgggccaattatttcgaaatcgtctcaagcttcaatctcaccctgcaccgctataaggtggaagtccaggaacaggacggtaaaacccctgctggcaaaaagcttaagcgtattatcgagcttctgttggaagagcacttcggaaacatgaaaaaccaaattgcaactgactacaaagcaacactggtctgcaaaaccgcaatcaaccttccccaggagatgttttcgataacgtatcgtctcgaatatgaagatgaacctgctcccagagccaggacttacaaggttcgggtcgtcgcaattggttctctcgatgtggcaccgttgctccaatatctctcctcacccaggagcggggaacaactcccggccaaagaagagatcatccaggcattgaatatcgtcctgggccacaaccccaagacaaatcgcgaattgctttcaattggcgcaaacaaacactttccacttggccggggcggggaaagctatgatctttctggtggactcactgcactcaagggcttcttcgtcagcgtccgtgccgcgacttcccgcctccttgtgaatgtccaagtcaaagctaccccgtgctacagtgaagggagcctgccagatattatgaacagcttttcagccagcatacgaggccctacgcactttcacaaactcaaccgattccttaagcgagtacgcgtgagcgtcaatcatatcgtccgcaaaaataaatcaggcagggtcatattgcgaatcaaaactattgaaggtcttgcacatcagagtgatgggcgtaatttgcagaacccgccacaggtaccttggctaggagcaggtccgaaagatgtcaagttctttcctgatggaccaacacccccggcagagggccaaagcaagaagaacgggaaaaaacagaaaaagggccctgtgaagccgggtggagattcttctacgggttcatatattagcgtctacgactattttaaacgcaattaccccaatatccccgagttgaacaacgcattaccagttgtcaatgttggcagcaaggaggagccgatctacctgcctgctgaggtctgccacgttctgcctggccagccggcaaatgccaaaatctccccccaacagactcagaacatgattcgctttgcagtccggagacccgtggaaaatgccaagtcgattgtcgtaaatggaacccaggtgctcgggtttaccccacatttgaatcctatgctggctggtatgggattatctctgttgccaaacttgattactgttcctggtcgagtgctggagggtcccgctattattcagtacaagggacagagctttaaaagccctcacggcgggaactggaaccttcagaaagtcgcattctcacatgggtcccagctacctgcctggacattcctttactaccaagacggccgaaccgatccagcggttgttatggagagcgttgatagatttatagatatggcaagggtacaagggctcgctgttccagtgcctagccggccaatcgctgtcaacgttccccctcgtcgagatgtggaggatataccactggaccccatctttgccgagattcgccagcagtcgcgagtgagattggttctggtgatcctaccttttgcaagccctcaaatttacagccaggtaaagtataggggagatatcaaggacggcatccatactatttgcgtagtcgcagaaaagttcaggaagaatcagcctcaatacttcgccaacgttgcgctaaaattcaacctgaagctcggaggagtcaaccacaaacttcagccgtccaaactgggggttatatcggaaggtaagacaatggttgtgggcattgatgtcacgcatcctgccccgggttcgctctccactgcaccaagcatagccgggatggttgcaagcgtggataaagtcctcggtcaatggcccgccaccatccgtcttcaacagcaggctcgcgcagagatggtgagtgatcttgatataatgctccaatctcgtcttcgcctttggcagaagaacaactccaagagcctcccggagaatatcctgatctaccgcgacggggtatcagaaggccaatacggcaaagtcctggacgaagaactacccctgctccgcagcgcgtgcaaagctatctacccagccgacaaaaccaagaaaggcctccccaaattcaccatcatcatcgtcggcaaacggcacaatacgcggttctacccaaccgatctcaaggacgcggacaacaactcaaatcccaacagcggaaccgtcgtcgaccgcggcgtcaccgaatcgcgccactgggatttcttcctccaagcccacaccgcactgcaggggaccgctcggccagcccactactacgtgatcctcgatgagatattcaccgggcggaagactgcaggccgcttcgacagcattgccgatgagcttgaagacctaacccataatctctgctacctgttcggacgagcaaccaaggccgtcagcgtctgcccgccggcgtactatgcggatctagtctgtgagcgcgccaggcgctacctaaatcgatatttcgacgtcacgcctgacaccagtgttgcttcgtctggtgctagtgatgtgggtcctgtgcctattggtgacgatgttcgcgtacattctgacctggtggatactatgttttatatctgagtgatatctgcgtacatatcgtgtcgaagggaaggggggtatcttgggatcggggaagttgtggaaaatgctatggaggttgtgaagtcagtatggaatagtacagtcattgctctgtcattagtggtattttgaaatcttgattttgctttttagggttggttattggtctatttaccgtgatcatatctgagtatatgattgggcacatatcaaattgataagttgacacagagctttacgagaatgcaattctaaactaatgtcttggatatttctcagagtttatctagcaaatgggtattaattgagtgatcagagttcaatcccatgcaaacctccaactactcaaatatactccgtgccaaaagctcattatcatcaccaaaatatccaccataccttcatattcatacatagagcgcacctgtcgaaatgcaaatgtacaaccttttaatcccaataaaaaaatcaaaagaaaaaaagccagctagagaaaaagagaaacctcccctccgccaatatcaccgaacaagccctactaccccccttccccccaacgaccgcacaacccccttcttcagccgaaactgcctctcaatcacctcctccgacacccctcccttatccctcatcgccaaaatatgcctaatactcacgacatccaaaaactcgcgcccgggatggctcggtcggccaacggactcgagttcgagttcggcagccttctctaaccgggcgcgggaggagagaacgaggagggctgggttggaggatgtggaggaggaggtggagggggggaagacgatggagagggattgctgaagctgcgggttttggggttggttgaaagggttgggggtgacgggcccgatggagcggagggaggcggcgaactgaggatcgcgggcttcgaggtcgatggctgtggttatttgaggccgggttcgctcgccttctcttctgcatggaatactggtcctggctgttcccgtcggcttgatgccctggccgctgggtccgcggctctggcaccaccgggcccggtggtcggtgcggagggtttcttggggtattgacgccgtgcagctgcattagctgccgcctttatgggtttcgacgatgatgcgcccattgcaattttgtgtgtgcaccgttactggggagtcggagaacgatactgtacagtaactagctcggtgagcggagaatgatggatggttttgcggagacggtccaagcttctacgggggccaaagctgacaggaatgtaggtttggtggaacgctaatttgcgtctagggctagaaggtggggcggggagagcagaataaataagacacacaggtgttctaggaatctcatgcatgggttctactaaataaataacgacctagagatacatctgttcatgaaaaaagcgctaggaagattccagctccaaagtgaagattctttgtcatatgccattttgccccgaatagcatgacgaaaaagcattagcaaaaccagaccccctagcgcagccctgcgaggcgcctctcctggacctcgct

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