LOCUS       XM_044424868            5245 bp    mRNA    linear   VRT 17-OCT-2021
DEFINITION  PREDICTED: Varanus komodoensis argonaute RISC component 4 (AGO4),
            transcript variant X4, mRNA.
ACCESSION   XM_044424868
VERSION     XM_044424868.1
DBLINK      BioProject: PRJNA771140
KEYWORDS    RefSeq.
SOURCE      Varanus komodoensis (Komodo dragon)
  ORGANISM  Varanus komodoensis
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata;
            Toxicofera; Anguimorpha; Paleoanguimorpha; Varanoidea; Varanidae;
            Varanus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_025335440.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Name             :: Varanus komodoensis Annotation
                                           Release 100
            Annotation Version          :: 100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 9.0
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Varanus komodoensis"
                     /mol_type="mRNA"
                     /strain="SLA01"
                     /db_xref="taxon:61221"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="blood"
                     /dev_stage="adult"
     gene            1..5245
                     /gene="AGO4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 7 Proteins, and 100% coverage of
                     the annotated genomic feature by RNAseq alignments,
                     including 6 samples with support for all annotated
                     introns"
                     /db_xref="GeneID:123020736"
     CDS             356..2344
                     /gene="AGO4"
                     /codon_start=1
                     /product="protein argonaute-4 isoform X4"
                     /protein_id="XP_044280803.1"
                     /db_xref="GeneID:123020736"
                     /translation="

MWNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKLTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQETSQELLYSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTQHTMYFA"

     misc_feature    407..769
                     /gene="AGO4"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(563..565,608..610,650..652,662..664,716..718,
                     737..739,743..745)
                     /gene="AGO4"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    908..2215
                     /gene="AGO4"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1319..1321,1331..1333,1367..1378,1385..1387,
                     1409..1411,1418..1420,1430..1432,1442..1444)
                     /gene="AGO4"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1523..1525,1529..1531,1769..1771,2183..2185)
                     /gene="AGO4"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

taaagtgtctgttcagtgggtgtctgtggtcagccttcagctgctcctggaagccctggctggtcatctgaatgaagtcccagaagattctgtacaagcactggatgtcatcacccggcaccttccttcaatgaggtgagatcccacaatctgttctcagactgcttacctgaagctaggtagactgccttgtgtgggtgctcatccgaattttccgctggtcttcctgaaatactaagtgaacaggtacaccccagtgggccgttccttcttctctccccctgaaggatactatcaccccctgggaggaggtcgggaggtctggtttggcttccaccagtctgtcaggcctgcaatgtggaacatgatgctcaacatagatgtatcagcaactgctttctaccgtgcccagcctattattgaattcatgtgtgaggtcctggatattcagaacatcaatgaacaaacaaaaccacttacagattcccagcgtgtaaaatttaccaaagaaatcagaggtctgaaagtagaggttactcactgtggtcagatgaagagaaaatacagagtttgcaatgttactaggcggccagccagccatcagacgtttcctctgcagctggaaaatgggcaggctatggagtgtacagtagctcagtattttaaacagaagtacagtctgcagctgaaataccctcatcttccatgcctacaagtagggcaggagcagaaacatacatatttaccccttgaggtatgtaacatagtagctggccagcgttgtatcaagaagcttacagacaatcagacatcaaccatgatcaaagcaacagcacggtctgcacctgataggcaagaagaaattagtaggctagtgaagagtaacagcatggtaggtggccctgatccatatctgaaggagtttggtattgtcgtccataatgaaatgacagagctgacgggcagggtcttacctgcaccaatgttgcagtatggaggcaggaacaagactgttgccactccaaatcagggtgtctgggatatgagaggaaagcaattctatgctggcattgaaattaaagtttgggctgttgcctgctttgctcctcagaagcaatgccgggaagacttgttgaagagttttactgatcagctgcgcaaaatctccaaggacgcagggatgccaatccaaggccagccgtgcttctgtaaatatgcccagggtgcagacagtgtggagcccatgttcaagcacttgaaactgacctacgttggactgcagctcattgtagtgattcttcctggaaagacaccagtctatgctgaggttaaacgtgttggggacactcttcttggcatggctacccagtgtgttcaggtgaaaaatgtggtaaaaacctctccacagactctgtccaacctctgtctgaagatcaatgcgaaacttggcgggataaacaatgtgcttgtaccacatcagaggccctccgtattccagcagccagtgatctttcttggagcagatgtcactcacccacctgctggggatgggaaaaagccttccatcgctgctgtagtaggcagcatggatggtcacccgagccgctactgtgcgacagtgcgcgtgcagacgtcccgccaggaaacctcccaggagctgctgtacagtcaggaagtgatccaggatctgaccaacatggtgcgagagctgctgatccagttttataagtctacccgcttcaaacccacgcgaatcatctactataggggaggtgtgtcagaagggcagatgaaacaggtagcttggccagaactaattgcaattcggaaggcctgcatcagcttggaagaagactacagaccaggaattacctacattgttgtgcagaaaagacatcatacaagactcttctgtgctgacaaaactgagagggtggggaaaagtggcaatgttccagcaggcacgactgtggacagcactatcacacatccctctgaattcgacttttacctctgtagccatgcaggaattcagggaaccagccgcccttctcactatcaagtcttgtgggatgacaactgtttcactgcagatgaattgcagctgctgacataccagttgtgccacacttatgttcggtgtactcggtctgtctcaattcctgctccagcatactatgccaggctggtagcatttagggcaagataccatcttgtggacaaagatcacgacagtgctgaaggcagccatgtttctggacagagcaacggccgagaccctcaagcactagccaaggcagtgcagattcaccatgacacgcagcacaccatgtatttcgcctgatgagaatgcgagatgatgaggcagaagcaccagcccacttgggcatgccaccaaccactggttgctagggctcagttttatttcaggctgctcaccaccagcagcttggggcagttgcactgaatcaacgtttgcagcaacatctgatacagacataattgagccatttttattttgtgattttccgtcaatagaaatttcatagacagtctttgcattggactgaatttttacctcaaaacgcaaaaggctgatcctcctagaataaatttttaaaggacttggcacgaaaggtttttattgaaatattttgctaaccagttttaagttacctccgcatccccattaagcttatcaagaagctgaattcctcctgggcttcttcatactgcttagtgagtatccagatatggcaaaacatcatattccacgttcttctgtgtggggccatatagactttataaacagaacttttacctgtaaacgcctttcaacaagcattaactttcagaggtatcgctggattattattattattattattattattaatattattattattgctttgtcaggttttcctagttctctttttgtaaatctctctgcagtcatgggctgcaaagcatttgcactataactgcgaattcaacttggtggttcttgttctgtgaatgtgtaacataagcagatagcaatgcccattggcaggtttactataatgaaatcataatggttacacacaactgccactgcatagttcagaatgctgaactgcttccccctctaaagtatgtggcaaagttctaaaatattagttttaggtatttttcagacattacaaaagtgcccattttatgctgctgtgtttgatgaattgaagtcatttttaatactctttttcatagaaggttttaactttggcaataatttttttttaaatacactggcagtgacttttctagttgaatactttacatgagttacagccagcattctgtgtaaattgacttatctttgctgaagagaaaattagttcttaaaaaggatcagaaaaggctcccttcatgctttttacaaatggttatttttaactgttttatattcaccattggtctgtctacccctttttataagcttggaaagtgggtgttctatataggaaaggctttgccaatatgtacagttgtgtatagtgtgacagtagcaattgaagacttttaccagcatttaaaaacatggtttttctgagaatttagattgccgcatgtacagcaacactcagtagcatgtgtgatgtgtacattttaatgtcgcttttgacccatttctataaattaggactcctatttgcttggctcttgtccactaaaatgtgtaatgcaaaaagctggggggggggacctgcagtagtgttgcattgagactagcttgtgagtgttagcacaagatgctttaaattcctagtttttaactgagatgatgccactgtgaaagattgggattttgccaataattgcactctagtctccaaaatgagcaggtgaggttgcagaattgtgtgaccttgagatcttttagatccaaacctcaaggtcagcaatatgctatgcggtttctatttttcttcagtcttttgaaataaagcttgtagtcttggaaagtttgtatttcccaataaggtactgggttaccactgtactggcatatactggtacagtgttaatcttcaaggagcagtttcataagctccttccggagctatatattgaaaagggtgggcttacaaagttttcccttccctgctcagaaataggagtgaagcaaactctgctgtgacacagtgtgaaaaggcttagatttttaaaaacttgttcaagtctttcttttttcctttaggaatacctcacttgggtaataaataatcaggatatgtggggagggagagtgaccgctgcttcagttagtctgctgcttttcttttcttttaatacttgttcagggagaaaatgcaagaatcgaactcggctggcccaaacatatggagaaccgaggcaccttgaagagagtttaagcacctgtttagtgtgtagcattttttattgcactaggtaaggacatggcttgagagcgaggaaggtacataaagaagcaaaatgcatgctttatcatcaagtaagcatgtagctgtcctagcaccaagcactcctagtgcatatggagccagagagacacccttatttgcttgtctgtagttaagaccaaccgttgtgaacattggtcagatgacacaagcacgagttttagagcttatgtttaccattggccattcttcctcagcaacagcagcttatgttctcacatgtggatcaggactacctgttcacgagcagagtggtttagcccctcccatgccattttcccttcacaaggagcacaaagcgccaccaaaattcgacacgaagcaaaactggcttctcatttctgctccagcatagctgcatttcacaaggcgagagagtgaccagttgtaaacacgcttttaaaagtgcatgcttaacataaccatagattggccgaaactggtgcagacaggaatcttagcaagccctagaagggcttttctggctacttcattgcaaaccaaggcctgggctggatgaaaaaggccttttatcatagggaaaaaactgttctgatcgttatatttttcttgtgtattacagactgcagctatcttctaaagtcttctgtatttaaatatatatatatatatctatatatataagcaggattctttttaatgcattattgttgcagtgaattttatcatccaaaaatgtctatggaaagaaactttttgccaacttttgcactttcttggatttttttttttttttt

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