LOCUS       XM_043873501            1897 bp    mRNA    linear   MAM 27-SEP-2021
DEFINITION  PREDICTED: Cervus elaphus engrailed homeobox 2 (EN2), mRNA.
ACCESSION   XM_043873501
VERSION     XM_043873501.1
DBLINK      BioProject: PRJNA764704
KEYWORDS    RefSeq.
SOURCE      Cervus elaphus (red deer)
  ORGANISM  Cervus elaphus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Laurasiatheria; Artiodactyla; Ruminantia;
            Pecora; Cervidae; Cervinae; Cervus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_057832.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Name             :: Cervus elaphus Annotation Release
                                           100
            Annotation Version          :: 100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 9.0
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Cervus elaphus"
                     /mol_type="mRNA"
                     /db_xref="taxon:9860"
                     /chromosome="18"
     gene            1..1897
                     /gene="EN2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 1 EST, 2 Proteins, and 100%
                     coverage of the annotated genomic feature by RNAseq
                     alignments, including 9 samples with support for all
                     annotated introns"
                     /db_xref="GeneID:122674889"
     CDS             488..1462
                     /gene="EN2"
                     /codon_start=1
                     /product="homeobox protein engrailed-2"
                     /protein_id="XP_043729436.1"
                     /db_xref="GeneID:122674889"
                     /translation="

MEENDPKPSEAAAAEGQRPPECSPSGGAGSPGEADTGRRRALMLPAVLQAPGNHQHPHRITNFFIDNILRPEFGRRKDAGTCCPGAGGGRGAGAGGEGGLEGGGGAGGAEQLLVSGREPRQNAPGAPGAGGPLPGGGGSDSPGDGEGGSKALSLHSGAKKGGDPGGPLDGALKARGLGGGDLSVSSDSDSSQASANLGAQPMLWPAWVYCTRYSDRPSSGPRSRKPKKKNPNKEDKRPRTAFTAEQLQRLKAEFQTNRYLTEQRRQSLAQELSLNESQIKIWFQNKRAKIKKATGSKNTLAVHLMAQGLYNHSTTAKEGKSDSE"

     misc_feature    order(1193..1207,1211..1213,1262..1264,1280..1282,
                     1319..1321,1325..1330,1337..1342,1346..1354,1358..1363)
                     /gene="EN2"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238039"
     misc_feature    1199..1360
                     /gene="EN2"
                     /note="Homeobox domain; Region: Homeobox; pfam00046"
                     /db_xref="CDD:425441"
     misc_feature    order(1199..1201,1208..1210,1328..1330,1337..1342,
                     1349..1351)
                     /gene="EN2"
                     /note="specific DNA base contacts [nucleotide binding];
                     other site"
                     /db_xref="CDD:238039"
     misc_feature    1364..1456
                     /gene="EN2"
                     /note="Engrailed homeobox C-terminal signature domain;
                     Region: Engrail_1_C_sig; pfam10525"
                     /db_xref="CDD:431338"
     polyA_site      1897
                     /gene="EN2"
                     /experiment="COORDINATES: polyA evidence [ECO:0006239]"
ORIGIN      

cggcgttggcccgagcgggccccggcgggcgtgagcgcgggcgagcgcgctgcctgcatgcgcggagctccagccccgggcggccgggcggcggcggcggcgccggagccggaggcagccggacgtggagaggcgcgcggagccccgaggcgccccgggtccgcgccggacccttgtgaccgtctagcgactgggtgcgctgcgaagactcggaccggactattttgaatctcccggcgtctgggcgtggggcgactcttggtattttctaactcaacttgtggatctgaacgtggctttggctctgagcgtggctggcgacgtgtaggaccccagccctggctgctgccgccgcgctcgccctcgggagagtcggcggggaagggacaccggccggcttcggaagtctccgcgtacggagcggagaagcggaaggctgactggagggtctccccggagaatcgactcgggatttgttgtgagcagcatggaggagaatgaccccaagcctagcgaagcggcggcggcggaggggcagcggccgccggaatgcagccccagcggcggcgccggcagccctggcgaggcggacactggccgccggcgggctctgatgctgcctgcggtcctgcaggcccccggcaaccaccagcacccacaccgcatcaccaacttcttcatcgacaacatcctgcggcccgagttcggccggagaaaggacgcagggacgtgctgtcccggcgccggaggaggcagaggcgccggggcaggcggcgaaggcggcctggagggaggcggcggcgcgggcggcgcggagcagctcctggtgtctggccgggagccccggcagaacgcgcccggggcgcccggggcgggcgggccgctgcccggcggtggcggtagcgactctccgggtgacggcgaaggcggctctaaagcgctctcactgcacagcggcgccaagaaaggaggagaccccgggggtcccctggacggggcgctcaaggcccgcggcttgggcggcggcgacctgtcggtgagctcagactcggacagttcgcaggccagcgccaacctgggcgcgcagcccatgctctggccggcttgggtctactgcacgcgctactcggaccgaccttcttcaggtcccaggtctcgcaaaccaaagaagaagaaccccaacaaagaggacaagcggccccgcacggccttcacggcagagcagctgcagcggctcaaggccgagttccagaccaacaggtaccttacggagcagcggcgccagagcctggcgcaggagctcagcctcaacgagtctcagatcaagatctggttccagaacaagcgcgccaagatcaagaaggccacgggcagcaagaacacgctggccgtgcacctcatggcgcagggcctgtacaaccactccaccaccgccaaggagggcaagtcggacagcgagtagggcgggcgggtgccacgtcccggcccgccctggctaaacgatgcaataatttaaaatcagaaatcaagggccagtgtataaagattataccagcattcatagtgaagatatcgtgtattagctatggttctgcaatattctatgtatatataatttacaggaggtgtaaaatccaaaatatctgactataaaatattttttgagtttttttatgtttatgagattatgctaattttatgttgggtttttggtttttgcaaagggggctacttagggtttcatcttttttaatcccctaagttccattatgggacatcggacactttttttttttaatttcaaaagatgaaaaaattaaaacaacttgctgaagtccaaagatttttattgctgcatttcacacgactgtgaaccgaataaatagctcctacttgg

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