LOCUS       XM_041565492            3927 bp    mRNA    linear   VRT 14-MAY-2021
DEFINITION  PREDICTED: Xenopus laevis argonaute 2, RISC catalytic component L
            homeolog (ago2.L), transcript variant X1, mRNA.
ACCESSION   XM_041565492
VERSION     XM_041565492.1
DBLINK      BioProject: PRJNA338693
KEYWORDS    RefSeq.
SOURCE      Xenopus laevis (African clawed frog)
  ORGANISM  Xenopus laevis
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae;
            Xenopus; Xenopus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_054381.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Name             :: Xenopus laevis Annotation Release
                                           101
            Annotation Version          :: 101
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 8.6
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Xenopus laevis"
                     /mol_type="mRNA"
                     /strain="J_2021"
                     /db_xref="taxon:8355"
                     /chromosome="6L"
                     /sex="female"
                     /tissue_type="Erythrocytes"
                     /dev_stage="adult"
                     /collection_date="06-Jul-2016"
                     /collected_by="Jessica Lyons"
     gene            1..3927
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="argonaute 2, RISC catalytic component L homeolog;
                     Derived by automated computational analysis using gene
                     prediction method: Gnomon. Supporting evidence includes
                     similarity to: 9 ESTs, 1 Protein, and 100% coverage of the
                     annotated genomic feature by RNAseq alignments, including
                     56 samples with support for all annotated introns"
                     /db_xref="GeneID:446823"
                     /db_xref="Xenbase:XB-GENE-1001922"
     CDS             296..2911
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /codon_start=1
                     /product="protein argonaute-2 isoform X1"
                     /protein_id="XP_041421426.1"
                     /db_xref="GeneID:446823"
                     /db_xref="Xenbase:XB-GENE-1001922"
                     /translation="

MYSGAGPVLVPPTTTPPLPMPAYTFKPPPRPDFGTSGRTIKLQANFFEMDIPKIEIYHYDIDIKPEKCPRRVNREIVEHMVQHFKAQIFGDRKPVFDGRKNLYTAMPLPIARDKQVELEVTLPGEGKDRIFKVAIKWMACVSLQALHDALSGRLPNVPFETVQALDVVMRHLPSMRYTPVGRSFFTASEGCANPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFMCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPFVREFGIMVKDDMTDVTGRVLQPPSILYGGRVWEEPNAPLSKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKTFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYTGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLSAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDQQALAKAVQVHQDTLRTMYFA"

     misc_feature    542..802
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    830..982
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    983..1345
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1139..1141,1184..1186,1226..1228,1238..1240,
                     1292..1294,1313..1315,1319..1321)
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1478..2782
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1916..1918,1928..1930,1964..1975,1982..1984,
                     2006..2008,2015..2017,2027..2029,2039..2041)
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(2120..2122,2126..2128,2336..2338,2750..2752)
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

ccttgacgttgcgtgtcacgcccccagcctctctccgccggccgttttcccctcagttcatgtcgcgcaaaagaaagaggaaaaaaaaaaaaagggtatagcagctagagaagcgagaggcgggaactcggcgtattccctcccacacaccgtgccttacaccatggagccataacggactgactggccgtctctgagggcttctgtgcggagcgaggggggcgggactggctacgttaccccctgtgtctggaactttattctcccctcacacttcgctgttgaagccgcagacatgtactccggggccgggccggtgcttgttccaccaaccacaacaccacctctcccaatgccggcatataccttcaagccacctccacggccagattttggtacctcagggaggaccatcaagctccaggcaaacttttttgaaatggacattcccaaaattgaaatatatcactatgacattgatataaaaccagagaaatgccccagacgagtaaacagggaaatagtggaacacatggtccagcactttaaagctcaaatctttggggatcgaaaaccagtgtttgatggaagaaaaaacctttatactgcaatgcctctgccaattgcaagagataaacaggtggagttagaagtcacactacccggcgaagggaaagatcgtatcttcaaagttgcaatcaagtggatggcatgtgtaagcttgcaagccctgcacgacgctctgtctggtcgtcttcccaatgtcccttttgagacagttcaggctttagacgttgtgatgagacatttaccttctatgaggtatacacctgtcggtcgttcgttttttactgcatctgaaggctgcgcaaatcctcttgggggtggtagagaagtttggtttggcttccatcagtctgtacgaccttccctctggaaaatgatgctcaatatcgatgtgtctgcgacagcattttacaaagcacaaccagtaattgaatttatgtgtgaagttctggattttaaaagcatcgaagagcaacagaaacctcttaccgattcccaaagggttaagtttaccaaagaaattaaaggtttaaaagtcgaaataactcactgtggacaaatgaaacggaaataccgagtttgcaatgtaacaaggcgaccagccagtcatcaaacattccctcttcagcaggagagtggacagacggtcgagtgtaccgtcgcacagtacttcaaggacagacacaaactagttctgcgctaccctcatttaccatgtttacaggttgggcaggagcagaaacacacgtacctcccccttgaggtgtgcaatatagtggcaggccaaaggtgcataaagaaacttacggacaatcagacctccactatgatacgggcaactgctcgatctgctccagatcgccaggaagagatcagtaaattaatgcgaagtgcaagttttaatactgatccctttgtgcgagagtttggtataatggtgaaagatgacatgaccgatgtcacaggcagagtccttcaacctccttcaatcctttatggaggcagggtatgggaagagccaaatgctcccttgagtaaagcaattgccacgcctgttcagggtgtctgggatatgagaaacaaacagttccacaccggcattgaaataaaggtgtgggccatcgcctgctttgccccacaacgacagtgtactgaagttcacctgaagacatttacagaacagttgaggaagatttctcgtgatgccgggatgcccatccaggggcagccatgtttctgcaagtatgcccagggagcagatagtgttgagccaatgttcaggcatctgaagaatacatatacagggctacaacttgttgttgtaatactgcctgggaaaacccctgtctatgcggaagtgaaacgtgtgggggacactgttctgggaatggctactcagtgtgttcagatgaaaaatgtgcagaggaccacaccacaaaccttgtctaacctgtgcttaaaaataaatgtaaaattaggaggcgtcaataacattttgttgccgcagggcagacctcctgtgtttcaacagccagtcatatttcttggagctgatgttacccacccacctgcaggggacggtaaaaagccttcgattgctgcggttgtaggtagtatggatgcacaccccaaccgatactgtgccactgtgcgggtgcagcagcaccgacaagaaatcattcaggatttgtctgccatggttcgggagctgcttatccagttctacaagtctactcgcttcaagcccacaagaatcatcttttacagagatggtgtctcagaagggcagttccaacaggttctccatcatgagcttctggccattagggaagcctgtattaaactggagaaggattaccagccaggaattacatttattgtagtgcagaaaagacatcacacgcggctattctgtacagaccgaaatgagagggtagggaaaagtggaaatataccagctggtaccactgtagacacaaagattacccacccttcagagtttgatttttacctgtgcagtcacgctggtattcagggaacaagcaggccgtcacactatcatgtcctgtgggatgacaatcgcttttcatctgatgagttgcagatcctcacttaccagctctgccacacttatgttcgttgcactcgttctgtgtctatccctgctccagcatattacgctcacctggtggcattcagagcaaggtatcatttggtggataaagagcatgacagcgctgaaggtagccatacatctggtcagagtaacgggagagatcagcaagcactcgctaaggcagtccaggtgcatcaggacacactgcgtacaatgtactttgcataacagccgctgtttatccagtgacttaatagcactcctggaaaaggattcacacagaccagctgcacttagttgtttgtaaactagtccatactacgctgactcccatcaaaaagtgccggtaaccctgttgtctcccttactgtccaaccagccttccgtcccaaccgcttatcacattttgtttccattcacaacgcagacctgtcacaattaatgtagcttggagtgtgcttcgtatgggaatgtttacaaaggtgcagagagaaaaacacacatacccatgtagaaaaactgaacacgtaacctcagggctttagactttcactatggaatattgtttcagtttctgttttatccctgttcagttgctgaatttaatgggatggttgcggtcagttgggttggttacaaaaagagcataaaatgagcttaaaaagaaaaaaaaaaggcattggcagaacatttgaagccttaaatattgagatttttaattagcaaatgaattttaccatatgtagctttttatgtgtcaaatgttctggtacataactttatgtatttcagagttatttttagtcaattagtttttaactgaatcgtagctacagaggattagtgccctattctctccaactcatgcttttacatataatatgatcttgtgccattcagaacactctctccatagaaagtctactttattgtacttgtgtctaaactctaacctttccaaagttgcttaccaatacgggtctgtggcaatcagtacaatcatgttaagctaatgtaaaaaaaaaaaaaatctatttaccagtattaatatgacatgctatttattttatttacaaaaaaatcataatttttttaactttattttttaaatagcaacatatctattctgtgcatgggttaaatcacctgacacgtttaacctttctttgacaatgtgacctgtgctacatggatttgttcttacaggctccaccgacagtgaatggattaaaaaaaacaaaact

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