LOCUS       XM_037827745            5906 bp    mRNA    linear   MAM 19-NOV-2020
DEFINITION  PREDICTED: Choloepus didactylus argonaute RISC component 4 (AGO4),
            transcript variant X6, mRNA.
ACCESSION   XM_037827745
VERSION     XM_037827745.1
DBLINK      BioProject: PRJNA678727
KEYWORDS    RefSeq.
SOURCE      Choloepus didactylus (southern two-toed sloth)
  ORGANISM  Choloepus didactylus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Xenarthra; Pilosa; Folivora; Megalonychidae;
            Choloepus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051308.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI
            Annotation Status           :: Full annotation
            Annotation Name             :: Choloepus didactylus Annotation
                                           Release 100
            Annotation Version          :: 100
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 8.5
            Annotation Method           :: Best-placed RefSeq; Gnomon
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Choloepus didactylus"
                     /mol_type="mRNA"
                     /isolate="mChoDid1"
                     /db_xref="taxon:27675"
                     /chromosome="2"
                     /sex="male"
                     /tissue_type="spleen"
                     /dev_stage="adult"
                     /country="Germany: Tierpark, Berlin"
                     /lat_lon="52.520008 N 13.404954 E"
                     /collection_date="03-Mar-2014"
                     /collected_by="Gudrun Wibbelt"
     gene            1..5906
                     /gene="AGO4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 8 Proteins, and 100% coverage of
                     the annotated genomic feature by RNAseq alignments,
                     including 8 samples with support for all annotated
                     introns"
                     /db_xref="GeneID:119527576"
     CDS             174..2090
                     /gene="AGO4"
                     /codon_start=1
                     /product="protein argonaute-4 isoform X5"
                     /protein_id="XP_037683673.1"
                     /db_xref="GeneID:119527576"
                     /translation="

MCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEISQELLYSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTQHTMYFA"

     misc_feature    174..515
                     /gene="AGO4"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(309..311,354..356,396..398,408..410,462..464,
                     483..485,489..491)
                     /gene="AGO4"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    654..1961
                     /gene="AGO4"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1065..1067,1077..1079,1113..1124,1131..1133,
                     1155..1157,1164..1166,1176..1178,1188..1190)
                     /gene="AGO4"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1269..1271,1275..1277,1515..1517,1929..1931)
                     /gene="AGO4"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

gtctgttcagtgggtatcagttgtgagcttacagttgcttttagaagctttggccgggcatttgaatgaagtcccagatgactcagtacaagcccttgatgttatcacaagacaccttccctctatgagtatctgcaactgctttctaccgggctcagcctatcattgagttcatgtgtgaggttttagacattcagaacatcaatgagcagaccaaacccctaacagactcccagcgtgtcaagtttaccaaagaaatcagaggtcttaaagttgaggtgacccactgtggacagatgaaacgaaaatatcgagtttgtaatgtgactagacggccagccagtcatcaaacttttcctttacagctagaaaatggccaagctatggaatgtacagtagctcaatattttaagcaaaagtatagtctgcagctgaaataccctcatcttccctgtctacaagtgggacaagaacaaaaacatacatacttgccacttgaggtctgtaatatagtggcaggacagcgctgtataaagaagctcacagacaatcagacttccacgatgatcaaagcaacagcaagatcggctcctgacagacaagaagaaatcagcagactggtgaagagtaacagcatggtgggtggacctgacccataccttaaagaatttggtattgtcgtccacaatgaaatgacagaactcacgggcagggtgcttccagcaccaatgctgcaatacggaggccggaataaaactgtagccacacccaaccagggtgtctgggacatgagaggaaagcagttttatgctggcattgaaattaaagtttgggcagttgcttgttttgcgcctcagaaacaatgtagggaagatttactaaagagcttcactgaccagctacgtaaaatctctaaggacgcaggaatgcccatccagggccagccatgtttctgcaagtacgcgcaaggtgcagacagtgtggagcccatgttcaagcatctgaaaatgacttatgtgggcctgcagctaatagtggttatcttgcccgggaagacaccagtatatgcggaagtgaaacgtgttggagatacccttctgggcatggccacacagtgtgtccaggtaaaaaatgtagtgaagacctcaccccaaactctttccaacctttgcctgaagataaatgcaaagcttggaggaattaacaatgtgcttgtacctcatcaaaggccctcggtgttccagcagcctgtcatcttcctgggagcagacgtcacacaccctcccgcgggggatgggaagaagccctccattgctgctgttgtcggcagtatggatggccaccccagccggtactgtgccaccgttcgggtgcagacctcccgtcaggagatctcccaggagctcctctatagccaggaggtcatccaggaccttacaaacatggttcgggagctgctgatccagttctacaagtccacgcgcttcaaacccactcggatcatctattaccgtggaggggtatccgagggacaaatgaaacaggtagcttggccagaactaatagcaattcgaaaggcatgtattagcttggaagaagattaccggccaggaataacctatattgtagtgcagaaaagacaccacacacgactcttctgtgcagataaaacagaaagggtagggaaaagtggcaatgtaccagcaggcaccacagtggatagcaccatcacacatccatctgagtttgacttttacctctgtagtcatgcaggaattcagggaaccagccgtccttcacattaccaagtcttgtgggatgacaactgtttcaccgcagatgagctgcagctgctgacttaccagctgtgtcacacttatgtgcggtgcacacgctcagtctctattccagcccctgcatattatgcccggcttgtagcatttagggcaaggtatcatctggtggataaagatcatgacagtgctgaaggcagtcatgtgtcaggacagagcaacggccgggatcctcaggccttggctaaggctgtgcagatccaccacgatacccagcacacaatgtattttgcctgagattcccagaaaaagaactcaaccaatttggcaccccatgcagcctcaaaatgtttcaaatgcctacagcctctagagagagctaagttgacttcaggtggtcttctaccagcagctcgaaatagttgcactgaatctatacttcacagcactgtctgacgcatcaacaaaattgagccattttttaaaaagaagtaatagatactcatagattgtcttttctgatgcactggactgaatttttacctcaatgtgcaaaggctgatcagcctgaactttctaaaggacttcccaagaagggtttctatggaatattttgctagctgtggtgttgttcatcacatccctccagtcttataaaagaagattattcctttttttttgtattcatcgagtggggcatatacctatatgggagagaaaaaccaatctacttcagttcagcataactctttaatcatgtgggtccatagactttttaatggagatttctgacttcaccactgcaaatgcctttaacaagcattaatttttgcaagttttacagagttttattaattttactactttatcttattgatctctgcagacttgtgctggtgcaagtacaggatgccaggcaattgcactatatttggctgcagattcagacaggcagttctcttatttggttggtttttgtgaatgtgtaacataaacaggtgtaatgcatgtcacagtgatagataacactgccatggtaaaagtactcatgttttgcccctctttgaaaaaaatcagcttttagtatttttccaagttttcagaagtgcccattttatgctgctgtgtggtttattagatctaaatgatttttaaactgttctcatagaaagttaactttggcaataaacattttttaaggtcttcctcttcttccccctgacagtgtagttttctagatgagatttcggtatgattttatcagctatttcttatatttcataatctggcaatttctgaaaccagtcagaaaagacatatatatccatgcctttttaaagaattatttttacctgttttatagccacaattgatgttctgtcactttgttttaaaataagctagaaccaggatgcttccttattggataggctttgccagtccttaaggtacattaaatgatgctgcaacttgcaaaatgtaccagcattaaaattgttttttctccctgggaatcaaggttactcacataattactaacagtgcaaattttttgcttctcttttaacctgaaatgtacttggtggggtttccttccttttggtaaactaaaactgtggagaaaagtttgctttttcttaggagattctggattccactcacagggagacagaattcctctcttgtccaaacaaagggagttgatctaaagcaagcaccaattcaaatataggggaaaatgtattctgtatttaatgtagataatactttgtgaatggacaacagttacatagagtcttggtagttccaaccagtgtttcccaagtccaatttttactgacttgtaattctctatcctggaacctcaggagtgagagaaggagagggggataaaaggcccagagccatactctatatggctgaggaaggtgctggaggaagcagctgacacaatgttgtaaaaaactattttgcctcctccgtccggaaactgctggtggggaaacactggatgtaatggagaagggtgtttgcttgaactcattagccagtccaaatctcctaaactagccttctcaaaatcccagcaatataaagcactgtttttcatcagtctttcaaacaagcttgtaaatatactgaagtttacatttctatgtgctgtcgatcagcggcaccaccagtgtttgctgagactggtccttcgagagaactttataacgtttgttgggtcctgtagctcattaagatgagtgcagacgttcttattctctgcagatagtgggaagaacacaagctctgttaccgaggcacaacataggcaggatggaagagatggcagatgctattttcttaagattttcttttcttttctttctttctttttttttttttttttgcaaatacctcacttggataacataaatcaggacatgttggtgagggactagctgctgcttttatcatacttgttcagggagagcacagaaaaatacccttcagcatggccactctggacccaagaggaggaaaattggaagttttttaggagaaggaggactcactagagttcaggatgtgaagaactctaggtggctggctggctggccagcaggcaccacacctgcacttgagtgcctcggacttgcttgttaattacgcacagaagaagcctcacttctcaccatctcctggcatgcttggagcagggagcaaagcagagtgcccggaagccctgttttcgcttgcctatggttgggctgtatgctcagcctcaaactatacagtttgtgataccttggtcagtcccagtacactggggcaaatcagtgtggtggaggaaaagccgggaggcctatttttatatgaaaacagtaccttgaagggtgctatgctttcattgcagacaaaaggaaagaaatacggaagaactagaattattggaaaattgctgaggggtgatctgtaagaggtaatcactgcacaatcagagttgcagcaatactaattgcaagcaagtctgaatgcatagttttaatttaattcagcttgacagtttgactacataaccccttgggactgaccgaatcgttatgcatgtgtcaatcagtgggaggtgtgcattctctagaagtagttttagggtgtttggttttaaaaaaaaaatgcagcctgttcaggcattgtcatatggtgtgaaagatttaaatgtagcaatgttaaatagtaacaatttggggaggagtggggctattaagagagtgaagactgaaatgttgaaccaattatgttttgttgttatggctgttagctgtttaattctgaatggtttataaagtgaactagctggctgaacgcagccagctgtctgggcaagcagagcctattcattcttactgtaagttctatttgctgcttccaaaggtaatgattttcaagcagacatgttctgtatggtctgtattttagcatctggtgcccagtctatatcagagtgtacttacctggcttagctacctacccttcctgtgggaaaaagtaatccttgtggagatacactgtttaacaaagctcaccctctttgccctgctctagcgaccttctgtggatgtgctgtaagattttcttgctcaatagcaaggtggtagctctgctttcattttaagaaagtggagacagagggcactgtatcaataccatttcaattccaaggatttttccttataaaattaaaggaaagatcttgttgattaacaattttcttatcccttgctattgacatgttcatgctcttcctatgtctggtggctgaaaatgtttgcatttgtttgcatttacctaatggtgtaatttttgtttctataattgttagacttgtaatgttttaaaatgtattttactatatttggactggatgtatgtctctagcaatacgaggtactttctaaactattaagggaggggttatatccccatgttgagataagatgatgatgattgtttaaattttgcaatgtttgttttgttttgttttgttttgttggcctgcagggatattttgtgttcatgtgtccaaaaaggaataaattggcattcttgtgccaaaagctgttt

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