LOCUS       XM_030774354            3550 bp    mRNA    linear   VRT 16-JUL-2025
DEFINITION  PREDICTED: Chanos chanos NRDE-2, necessary for RNA interference,
            domain containing (nrde2), mRNA.
ACCESSION   XM_030774354
VERSION     XM_030774354.1
DBLINK      BioProject: PRJNA564626
KEYWORDS    RefSeq.
SOURCE      Chanos chanos (milkfish)
  ORGANISM  Chanos chanos
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Gonorynchiformes; Chanidae; Chanos.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_044495.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_902362185.1-RS_2025_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.4
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/15/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Chanos chanos"
                     /mol_type="mRNA"
                     /db_xref="taxon:29144"
                     /chromosome="1"
     gene            1..3550
                     /gene="nrde2"
                     /note="NRDE-2, necessary for RNA interference, domain
                     containing; Derived by automated computational analysis
                     using gene prediction method: Gnomon. Supporting evidence
                     includes similarity to: 6 Proteins, and 100% coverage of
                     the annotated genomic feature by RNAseq alignments,
                     including 1 sample with support for all annotated introns"
                     /db_xref="GeneID:115811930"
     CDS             21..3500
                     /gene="nrde2"
                     /codon_start=1
                     /product="protein NRDE2 homolog"
                     /protein_id="XP_030630214.1"
                     /db_xref="GeneID:115811930"
                     /translation="

MALFPAFGGASNTKVESSRKDLEWLSNQSFHTDDALTLHQRVVEKASTEAQGLSETRDQKHQKEEHGCDSDEYIRSKRKKKKEKKKKRKKQKKRTGGSSESSSSESDTVYPSDLLKKAESAERQESQVQPGDSFAWLDDLQGSTEKLFCIDRRADPANWEYKSLYRGDIARYKRKGSSSLGLDSKTQAVSWNDSGPEKKRVDRKPERYFSSSFRQVLRSDGQPALPALTEPLTNTPFIPLPAYPEDQNPAAQQPSSWVNPLGVYDSSTTLWLQGKGQAEVKGQQQRGEEPAGVGGGPLMARVEEFNRKLRENPTETHTWLEFIRFQDELAAHSGTFSGAQDSDAERRRGSVRALLEKKVAIAERAVEANPSCVEVKLERLRLCKELWEPATLLKEWKKLVFLHPNSAPLWRHYLLFTQSHFSTFSVSKVNAVYGKCLSTLSSVLDGSMVSHPPLPGTEEDMLAIFLQQCHFLRQAGHSEKAVCLFQALLDFTFYKPDSVKELPTRQQVEFFEPFWDSGEPRVGEHGARGWKAWMHQQERGGWVVPPEAEDDEDEDEEDSEIKDKSWPKWKIWLDVESSREAKHWLPWRPDKAKGQSEEDCEDPDRQVLFDDIGSSMIRIRSPALQLRFILSFLQFLGLPGPVGTTRSPSHSLILDDLSLLAEGPDPERPLTSYDSLKTGVCSVGHMTFLLDPRRQPGLCKSGEEFVRNVLEQIMPLVSVQDRAALSLCWLQYEKLKVLRCVCSRNKKRLKAQGKRSKRLAKRLLKEPENRGSLALWREYAHLEWLLGNLEEARKVFDTALGLGISRGLADPDLCDLCLLYAQLEVEDAWRVGAQNSGTPPTSSSAVHVLTKLAEGTGYAPFSGQINPVTILKARKTYEQTLMASLPGQEDAVSHGGSKKLRRVAALVGCFGLFQYLTMGIDAADAVYSQARDRLAERSAMEGSSDSETPSECEAVTVQHLALLKHHVSTNVCPLSRLRLALTSSLTRLPSSAPLWQLYIQVESRYHSAGRSRRFFHSVAKDNRSVIPHLFAITAEQRRKRLLDSVQRSGLPGEVLPTLPENGLSNRIRSLFEVATATETGAHCPLLWRMYLNFMVSDGNAERGRGIFYKALQDVPWVKGLYMDAVQLFPEHIQEFLDLLTEKELRLRAPMEEVDILLED"

     misc_feature    462..743
                     /gene="nrde2"
                     /note="MTR4-interacting domain (MID) found in nuclear
                     exosome regulator NRDE2 and similar proteins; Region:
                     NRDE2_MID; cd22200"
                     /db_xref="CDD:412062"
     misc_feature    order(462..485,492..497,507..512,516..518,522..566,
                     573..581,585..593,615..623,633..638,642..647,654..674,
                     702..713,717..743)
                     /gene="nrde2"
                     /note="MTR4 binding site [polypeptide binding]; other
                     site"
                     /db_xref="CDD:412062"
     misc_feature    930..1931
                     /gene="nrde2"
                     /note="necessary for RNA interference; Region: NRDE-2;
                     pfam08424"
                     /db_xref="CDD:462472"
ORIGIN      

ttttcattgaaaagagcaacatggccttattccctgcgtttgggggagcctcaaacacaaaagttgaaagctcccgtaaagacctggaatggctgagcaatcagagtttccacacagatgatgccctgactctccatcagagagttgtggagaaagccagtactgaggctcagggcttgtcggagacaagagaccaaaaacatcaaaaagaagagcatggctgtgatagcgatgagtatatcagatccaaaaggaagaaaaagaaggaaaagaagaagaaacgaaagaagcagaagaagaggaccggggggtcttctgagagcagcagctcagagtcagatacagtctatcccagtgacctgctgaaaaaagcagagagcgctgagcgacaggaatctcaagttcagcccggagattccttcgcgtggctggatgacctccagggctccaccgagaaacttttctgcatcgacaggagagcagatccagccaactgggaatacaagtctctctacagaggagatatagccaggtataagagaaaaggcagctcctcgttgggactggacagcaagacccaggctgtgagctggaatgactctggtccggagaagaaacgcgtggacagaaagccagaacggtacttctcctcaagtttccggcaagttctgcgctcagacggccagccagctctgcccgcactaacagaacccctgaccaacacccctttcattccccttcccgcctacccagaggaccagaaccctgccgcgcagcagcccagctcctgggtcaacccactaggtgtttacgactccagcaccaccctctggctccagggcaaaggtcaggcagaggttaaaggccagcagcagcgaggcgaggagccggcgggggtcggaggcggaccgctcatggcacgcgtggaggagttcaataggaaactgcgggagaatcccacagaaacgcacacatggctggagttcatcaggtttcaggatgagctggcagcccattccggcactttctccggggcgcaggacagtgacgcggagaggaggcgcgggtcggttcgtgctttgctggagaagaaggtagccattgccgagcgggccgtggaggccaatcccagctgcgtggaggtcaagttggagcgtctcagactgtgcaaggagctttgggaaccagccacactgctgaaagagtggaagaaactggtattcctccacccaaacagtgctcccctttggagacactacctcctcttcacacaaagtcactttagtactttctcggtgtccaaagtcaacgcagtctatggaaagtgcctgagcactctgagttcagtgctggatgggagtatggtttctcacccaccattgccaggcactgaggaggacatgctagcaatcttcctacagcagtgccatttcctgcggcaggctggccactcagagaaggctgtctgtctcttccaagctctgctagacttcaccttctacaagccagacagtgtcaaagagctacccaccagacagcaggtggagttttttgagccattttgggacagtggggaacccagggttggagagcatggggccaggggctggaaagcttggatgcatcagcaagagagaggaggctgggtggtccctccagaggctgaggatgatgaagatgaggacgaggaagattctgagatcaaggacaaatcctggccaaagtggaagatctggttagatgtcgaatcgtctcgggaggcaaaacactggttaccttggagaccagacaaagcaaagggccaatcagaagaggactgtgaagaccctgacagacaggttctctttgatgacattgggtcttctatgatcaggataagaagcccagccctgcagctacgctttatcctctcctttcttcagttccttggcctgccaggacctgtgggtaccaccagaagtccctcccacagtctcatcctggatgacctctcactcttggctgaaggtcctgaccctgaaagacccctgacctcttatgactctctaaagacaggagtctgctctgtgggtcacatgacattcttgctcgaccccaggaggcagccaggcctgtgtaaatcaggggaggagtttgtgcggaatgttctagaacagattatgcctctggtgtctgtgcaggacagagctgctctctccctctgctggctgcagtatgagaagctcaaggtgctgaggtgtgtttgcagcaggaataagaagcgtttgaaagctcaaggtaaacgcagtaagaggctggcaaagcgtttgctgaaggaaccagagaacagaggcagtttggccctctggagggagtatgcccatctggagtggcttctgggtaatctggaggaggcacgcaaggtgttcgacacggctctgggactcggcatatctcgaggcttggctgatcctgacctttgtgacctctgcctactttatgcccaattagaagttgaggacgcctggagggttggtgcacaaaactctgggactccccccacctcctcttcagcggtccatgtgctcaccaaattagctgagggtacagggtacgctccattctcaggccaaattaaccctgtaactatactaaaagctaggaaaacctatgagcagaccctaatggccagcctgccaggacaggaagatgccgtttcccacgggggcagtaagaaacttaggagagtggctgcactggttggttgttttggactctttcagtatttgacaatgggaatagatgctgctgatgcggtctatagccaagcaagggacagactagctgagagatcagcaatggagggcagcagtgactcagaaacaccctcagaatgtgaggctgtgactgtccagcatctagcgctgttaaaacaccatgtcagcaccaacgtgtgcccactgtcacgactcagactggcgctgacctcttcactcacgcgtttgccctccagcgcccccttgtggcagctgtacatacaggtcgaaagccgttaccatagcgcaggccgctcccgccggtttttccacagcgtggccaaggacaaccggagcgtcatcccacacctgtttgccatcactgcggaacagcgtcgaaaaaggctgctggattcagtgcagaggtctggcctccctggggaagttctccccaccctccccgaaaatggcctcagtaaccgtatccgctcactttttgaggttgccacggcaaccgagactggggcacactgtcctttgctatggaggatgtacttgaacttcatggtgtctgatgggaatgcagagagaggcagaggaatcttctataaagcacttcaagatgtaccatgggtaaagggtttgtacatggacgctgttcagctcttccctgagcacatccaggagttcttagacctcctgacggaaaaggagctgcgactgagggcgcccatggaggaggtggacatcctgctggaggactaacaaaaacacatctttgccaaagaaatctcagctttggacattctgttcat

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