LOCUS       XM_026300192            5739 bp    mRNA    linear   VRT 08-JUL-2025
DEFINITION  PREDICTED: Mastacembelus armatus argonaute RISC component 4 (ago4),
            transcript variant X2, mRNA.
ACCESSION   XM_026300192
VERSION     XM_026300192.1
DBLINK      BioProject: PRJNA489256
KEYWORDS    RefSeq.
SOURCE      Mastacembelus armatus (zig-zag eel)
  ORGANISM  Mastacembelus armatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Neoteleostei;
            Acanthomorphata; Anabantaria; Synbranchiformes; Mastacembelidae;
            Mastacembelus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_046643.1) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_900324485.2-RS_2025_07
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.4
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 07/03/2025
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Mastacembelus armatus"
                     /mol_type="mRNA"
                     /db_xref="taxon:205130"
                     /chromosome="11"
     gene            1..5739
                     /gene="ago4"
                     /note="argonaute RISC component 4; Derived by automated
                     computational analysis using gene prediction method:
                     Gnomon. Supporting evidence includes similarity to: 4
                     Proteins, and 100% coverage of the annotated genomic
                     feature by RNAseq alignments, including 5 samples with
                     support for all annotated introns"
                     /db_xref="GeneID:113126231"
     CDS             171..2795
                     /gene="ago4"
                     /codon_start=1
                     /product="protein argonaute-4 isoform X2"
                     /protein_id="XP_026155977.1"
                     /db_xref="GeneID:113126231"
                     /translation="

MEALGPGPPAPTSLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDIDIKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDRVDLEVTLPGEGKDQTFKVSLQWVSVVSLQMLLEALSGHLNEVPEDSVQALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPVIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNDMTEVMGRVLPAPMLQYGGRVSTDTGRDCGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAPQKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMSYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQDMSQEQLFSQEVIQDLTNMVRELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCSDKAERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHYDTQHTMYFA"

     misc_feature    252..641
                     /gene="ago4"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    672..824
                     /gene="ago4"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    825..1187
                     /gene="ago4"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(981..983,1026..1028,1068..1070,1080..1082,
                     1134..1136,1155..1157,1161..1163)
                     /gene="ago4"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1326..2666
                     /gene="ago4"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(1770..1772,1782..1784,1818..1829,1836..1838,
                     1860..1862,1869..1871,1881..1883,1893..1895)
                     /gene="ago4"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    order(1974..1976,1980..1982,2220..2222,2634..2636)
                     /gene="ago4"
                     /note="active site"
                     /db_xref="CDD:240015"
ORIGIN      

cagcagcagcggtagtccgggtcagtcagtccggtcaacaggcgtccacgcagctgtttaaattcccttttctttagttagtcactttaagcttcgcgataccgcccccaatttcagtgtcggtacggagccgccaggccgggacagagacggagagagaggcctcggccatggaagcgctcggacccggcccgcctgcccctacctctctgtttcagcctccacggcgtcccggccttggcacggtggggaaacccatccggctccttgccaaccacttccaggttcagattcccaagattgatgtctatcattatgatatcgacatcaagcctgagaaacggcctcggagggttaacagggaggtggtggacaccatggtgcggcacttcaagatgcagatctttggagaccgacagcctggctatgatgggaagaggaacatgtacacagcacatccactgccaatagggagagacagggtggatctggaggtgaccctgcctggcgagggaaaggatcagacgttcaaagtgtctttgcagtgggtgtctgttgtcagtcttcagatgctgttggaagccctgtcaggtcaccttaacgaggttcccgaagactccgttcaggccctggatgttatcacacggcatctgccttccatgaggtacactccagtggggcgttcatttttctcgcctccggagggctattaccacccccttggtggaggcagggaggtgtggtttggtttccatcagtccgtccgtcctgccatgtggaacatgatgctcaacattgatgtgtcagccactgcgttctaccgtgctcagcctgtgatcgagttcatgtgcgaggtgctggatatacagaacatcaacgaacagacgaagccacttactgactcgcaacgtgtcaaattcaccaaggaaataagaggcctaaaagttgaggtcacacactgtggtcagatgaagagaaagtatcgtgtgtgcaatgtcacacgcagacctgccagccatcaaacgttccccttacagcttgagaatggccaagccatggagtgcacagtagctcagtatttcaaacagaagtacaatctgcagctcaagtatccacatttaccctgtctgcaagtaggtcaggaacagaagcacacctaccttcccctggaggtctgtaacatagtagcaggccagcgctgtatcaaaaaactaaccgacaaccagacatccaccatgatcaaagctacagctcgatcggcccctgacagacaagaagagatcagcagactggtcaaaagcaacagcatggtcgggggcccagacccttatctaaaggaatttggcattgtagtgcacaatgacatgacagaggttatggggcgtgttctccctgcgcccatgctgcagtatgggggccgggtgagtacagacacagggagggactgtggcaggaacaaaaccgttgccacacccaatcagggcgtgtgggacatgagggggaagcagttctacgccggcatcgagatcaaggtctgggctgtggcctgcttcgctccacagaaacagtgtcgggaggacctgctcaagagcttcactgaccagctgcgaaagatctccaaggatgctgggatgcccattcagggccagccgtgtttctgtaaatacgcccagggagctgacagtgtagagcccatgttcaaacacctcaaaatgtcttatgttggtctgcagctgatagtggtcatcctgcctggcaagacgcctgtctatgctgaggtgaagcgggtaggtgacaccctcctcggcatggccacccaatgtgtgcaggtgaagaatgtagtgaagacgtccccacaaaccctttccaacctctgcctcaagatcaatgccaagctgggaggcatcaacaatgtcctggtgcctcatcagaggccctctgtgttccagcagccagtcatcttcctgggtgctgatgtgacccatcctccagctggtgatgggaagaagccatcaatagcagcagtagtgggcagcatggatggccaccctagccgttactgcgcaacagtgcgagtccaaacatcccgacaagatatgtcccaggaacaactattcagccaggaggtaatccaagacttgaccaacatggtgcgtgagctgctcatacagttctacaagtccacccgcttcaagcccacacgcatcatttattatcgcggtggtgtgtcagagggacaaatgaaacaggtggcgtggccggagctgattgccatccggaaggcatgtatcagtctggaggaggattacagaccaggcatcacctacatcgtggtccagaaacgtcaccacactcgactcttctgctccgataaagctgagagggtggggaagagtggtaatgtccccgctggcaccacagtggacagcaccatcacacatccatctgagtttgacttctacctgtgcagccatgctggtattcagggaaccagtcgtccctcccactaccacgtcctgtgggatgacaactgtttcactgcagatgaactgcagctgctcacctaccagctgtgccacacctacgtccgctgcacacgctccgtttccatcccagcaccagcttattatgccaggctagtggctttccgagcccgctaccacctggtggacaaagaccatgacagtgctgaaggcagccacgtgtcgggccagagtaacggccgggacccacaggccttggcaaaagcggttcagatccactatgatacccagcacaccatgtactttgcctaagcaaatgagcacatcagccagtcaacccacagattccctttctttctgtcttttctgctctgatttgttaatttctcttcttctccagtgtctctcttcttgtagtctgtctttgtcttcacactcactgtacatttctgaatctgcaccaaagcagctcttggacagaaaaatctgctgcatctcgatatccagcaggttctgtttcatggaacagtctccagccagcgttcccagtcgagctgccttcctaaccagcaatccagcactgtgaaccgtaagggttcccagtgcacaaaagaaaaaacatgaaaaaacaaaaacaaaccataaacacatactacaaagttgagccattattttgagtttgtttttgttttttctatttgaatgtctgcactcgtgttttaagatacactgagcaagggagtgaattggcacaatatgtgtcagcgcagctctttagctctcccagctaagcaggactcttatctacttttacctcaacgcccctttgggcaaaatctgtcacaaggtcttgggtttattgggtggcggggagggaactctacatgaaaagagatggaggggagtcgaccttttgagaatgatatatatatatattttttcctttgtatgagcgtatgttttaactttctcctttacagtcttttacaagcgaggtgacctgtgtgtccatgcccattactactttcttcccatcttaccgaagtatagacaagtttgtgtgtgtgtgtgtgtctgtgtggaaaaagtctctgtatatacgtgtactgctgtatgagtgtttgtgtgcatgtgtttcatggcaggccactgaattgtaaagggaaatcaaggagataactgctgtaaatgcctcagatttgttgtttttatattcacacatacagtacatataagcatatatttacatctatagagcaactttgagacatatgacttaaacaggcctcagaggctggtgtgggtatttgtagcagtcctggctcacttattaattgaaatgcattgagttacgttttgaacatgtgcattagtctgctgctagtgcacgattggtgggatttgcaccactggaaaccagctgttaataactaagttaatccagttcaactaataagtgaagtcaggtctgatgtgtagcagtagtgaccgtggtctggcctgtgactggctcagcattgggacctgttaaatggctgaaggcattaaacacaagagtatggtcttatcacccttgctttctgtgttaaaaatgggatcgtacttttctttggacctgactatctgaaaaattatattggatttttttttttttttttttttttaaactaatttaaacggcccaaaaccagcatagctttttttttttcttttctttttttttttttaaaaaaggtctcctttgctaatagtggccaattcattgcactgacccgggatgctaggcaagcaacagagggcactctcaaactctcttacctttatgcatttatacatacgaatcaacaaagcagatttgtaaaagtttaatataagatgttttgtggctatcttaaaaaatgttgtttagaattagtagtttaccattgccgttgttatcgttgttgtcaatgttgttactattgtgatgaaattttaaggtttgcagcaaagccagttatatagtttcttgtgtgacttcaggaatagacatcagttgttgagctgcaggttaaggggtagagctttgaattggaagttttttctgtagcattcgggcaaatggtgagaagtagttttccttgggatggctgaatctatgcttcctctctaggttcttatgtcagtaaaggtaagcagcagactggaggtgttttgtagcaccctgaatggttagacagatgtgttcactcggctggtattggtttggatcaaatggatggagacaatcggggattgtagtcagagtgaacctagattaagggatttgttaaaagtggtttgccataggcagtgagtcgaaattgtagactttttaaaaatggtttctattactgtgtatttgaagtatacccatcttaaagtctactatttaatggtgtttgtttcatatttttatgcatttttaacactggattaaatgaattgacaataagcataagcactgcccttggacccccaaacaaacactgagcaaactgttgccatagaaatgatggtgacagctgtgccagtggttctcccttgtgttgtgattggataacactgtaggacagagggtttggagaaatggtcgacgtgcacgcctcatcggggggacagctcactgaaaacccttttgtaataaaccacccataaagcagcaggtttactatgaatgtgtttgagcacctgcctgttgaaggcgtgcgcgttggcgtcggttaagagcgaagagcaggtagtgaggagggggaacggggcgggtgttgtccaggcattgcatgcaataagttttactctgttttaaaggggagagatcggtttttaaatgtgcactagttttaccaagcgacgtgggctgtgattaaatattcatctggtttctctaatggagaataataatttttttttttttttttttttttaaattgcagtgcttcatcattaaactccactcagataaattatcactccctgtttgtaccatggcattgcaaagcaatattaacccagttctagttttcaaatgtggaaagacaaatctataattctactcattgtggttgttggtctactctcgctattgtctctgtacttttattttgtattgttttactttttttggttattttctttgcttgtatcaatcagtgggaatgtgcatttggtggaatagttatataggctttagacaattttctttacagtaataaaatgtagactttgctcgcctgca

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